Cultured monkey chondrocytes showed a significantly reduced response to des(1‑3)IGF-1 with increasing donor age.
In plain wordsIn cells from monkeys, the response to des(1‑3)IGF-1 decreased with donor age.
- Peptide
- IGF-1 DES (des(1-3)IGF-I)
- Outcome
- DecreaseChondrocyte response by age
- Study
cynomolgus monkeyn = 34
primary chondrocytes cultured in alginate- Comparator
- younger donor age
- Outcome group
- Bone and joint · Musculoskeletal
Source sentences
There was a significantly reduced response to des(1-3) IGF-1 with increasing age, but no effect of OA score on response to des(1-3) IGF-1.
4 context sentences(design, population, supporting numbers)
Chondrocytes were isolated from cartilage removed separately from the medial and lateral femoral condyles and tibial plateaus of cynomolgus monkeys at the time of necropsy.
Isolated chondrocytes were cultured in alginate in serum-free medium and stimulated with IGF-1 or des(1-3) IGF-1, which has a much lower affinity for IGF binding proteins (IGFBP) than IGF-1.
Response was measured as the ability to stimulate sulfate and proline incorporation.
Cartilage samples from 34 monkeys ranging in age from 6.7 years to 27 years and with histologic scores ranging from 0 to 7 were analyzed.
In this study, IGF-1 DES (des(1-3)IGF-I) was a lab tool or a carrier, part of a mix, or the subject of a lab method. This result is not evidence about taking IGF-1 DES (des(1-3)IGF-I).