In LHRH-receptor-positive cancer cell lines, cetrorelix reduced EGF-induced c-fos mRNA expression dose-dependently versus EGF stimulation alone.
In plain wordsIn cells expressing LHRH receptors, cetrorelix reduced EGF-induced c-fos gene expression dose-dependently compared with EGF stimulation alone.
- Peptide
- Cetrorelix
- Outcome
- DecreaseEGF-induced c-fos mRNA expression
- Study
humann: not reported in source
Human endometrial, ovarian, and breast cancer cell lines- Comparator
- EGF stimulation without LHRH analog
- Outcome group
- Receptor activity and mechanism · Cancer
- Studied dose
- Dose not stated
Source sentences
In all of the lines expressing LHRH receptor, EGF-induced c-fos mRNA expression as well as c-Fos protein synthesis was dose-dependently reduced by treatment with LHRH agonists and antagonists.
2 context sentences(design, population, supporting numbers)
Human endometrial (Ishikawa, Hec-1A), ovarian (EFO-21, EFO-27, SK-OV-3), and breast cancer cell lines (MCF-7) were rendered quiescent by incubation (72 h) in the absence of fetal calf serum and phenol red.
This was followed by a 15-min incubation in the absence or presence of the LHRH agonist triptorelin (100 nM) or the antagonist Cetrorelix (100 nM) before the cells were stimulated for 10 min with EGF (100 nM).