In bovine cartilage discs, des-(1-3)-IGF-I did not compete with radiolabeled IGF-I for cartilage binding even at 100-400 nM.
In plain wordsIn lab tests, des-(1-3)-IGF-I did not reduce radiolabeled IGF-I binding to cartilage, even at the tested high concentrations.
- Peptide
- IGF-1 DES (des(1-3)IGF-I)
- Outcome
- No significant changeCompetition for cartilage binding
- Study
bovinen: not reported in source
Articular cartilage discs- Comparator
- Radiolabeled IGF-I binding without competitor
- Outcome group
- Receptor activity and mechanism · Musculoskeletal
- Studied dose
- 100-400 nMAs given in this study (bovine).
Measurements
- Competitor concentration
- 100 to 400 nM
- des-(1-3)-IGF-I; no competition
Source sentences
By contrast, an analog of IGF-I with very low affinity for the insulin-like growth factor-binding proteins (IGF-BPs), des-(1-3)-IGF-I, was not competitive with (125)I-IGF-I for cartilage binding even at 100-400 nM.
1 context sentence(design, population, supporting numbers)
Bovine articular cartilage discs (3 mm diameter x 400 micrometer thick) were equilibrated in buffer containing (125)I-insulin-like growth factor (IGF)-I (4 degrees C) +/- unlabeled IGF-I or IGF-II.