Oxytocin receptor binding in rat and human heart.

Cicutti NJ, Smyth CE, Rosaeg OP, Wilkinson M

The Canadian journal of cardiology1 Nov 1999Cited by 20 (Europe PMC)

PMID10579742PepBankPUB-10579742

Summary

The text of this summary is not shown here, because the article license does not allow reuse. Read it at the source (opens in a new tab)

Text rights: Publisher copyright. Results still quote short attributed sentences.

Results from this study7

7 results from studies that gave the peptide. No result from this study comes from people.

01LabEx vivo tissueActivity measurement
In rat left ventricle tissue, [3H] oxytocin bound saturably to a high-affinity site.
In plain wordsIn lab tests on rat heart tissue, oxytocin bound tightly to a specific site.
Peptide
Oxytocin
Outcome
Not applicableHigh-affinity [3H] oxytocin binding in rat left ventricle
Study
ratn: not reported in source
Rat left ventricle micropunches
Outcome group
Receptor activity and mechanism · Cardiovascular
Studied dose
Dose not stated
Measurements
KD
≈ 1 nM
high affinity site, rat left ventricle
Bmax
≈ 1480 fmol/mg protein
high affinity site, rat left ventricle
Source sentences
Summary
Saturable, high affinity binding of [3H] oxytocin to rat left ventricle was observed, consisting of a high affinity site (affinity [KD] approximately 1 nM; receptor density [Bmax] approximately 1480 fmol/mg protein) and a higher capacity, lower affinity site (KD approximately 75 nM; Bmax 3730 fmol/mg protein).
1 context sentence(design, population, supporting numbers)
Summary · context
A radioligand binding assay was used to characterize and quantify specific binding of [3H] oxytocin to standardized micropunches (thickness 400 microm; diameter 2 mm) obtained from rat left ventricle and human right atrium.
Report a problemFND-1057974201
02LabEx vivo tissueActivity measurement
In rat left ventricle tissue, [3H] oxytocin also bound to a higher-capacity, lower-affinity site.
In plain wordsIn lab tests on rat heart tissue, oxytocin also bound to a second site with weaker grip but more capacity.
Peptide
Oxytocin
Outcome
Not applicableLower-affinity [3H] oxytocin binding in rat left ventricle
Study
ratn: not reported in source
Rat left ventricle micropunches
Outcome group
Receptor activity and mechanism · Cardiovascular
Studied dose
Dose not stated
Measurements
KD
≈ 75 nM
lower affinity site, rat left ventricle
Bmax
3730 fmol/mg protein
lower affinity site, rat left ventricle
Source sentences
Summary
Saturable, high affinity binding of [3H] oxytocin to rat left ventricle was observed, consisting of a high affinity site (affinity [KD] approximately 1 nM; receptor density [Bmax] approximately 1480 fmol/mg protein) and a higher capacity, lower affinity site (KD approximately 75 nM; Bmax 3730 fmol/mg protein).
1 context sentence(design, population, supporting numbers)
Summary · context
A radioligand binding assay was used to characterize and quantify specific binding of [3H] oxytocin to standardized micropunches (thickness 400 microm; diameter 2 mm) obtained from rat left ventricle and human right atrium.
Report a problemFND-1057974202
03LabEx vivo tissueActivity measurement
In rat left ventricle tissue, [3H] oxytocin binding was displaced by oxytocin, vasopressin and an oxytocin agonist but not by the antagonist atosiban.
In plain wordsIn lab tests on rat heart tissue, oxytocin binding was blocked by oxytocin, vasopressin and an agonist, but not by atosiban.
Peptide
Oxytocin
Outcome
Not applicableDisplacement of [3H] oxytocin binding
Study
ratn: not reported in source
Rat left ventricle micropunches
Outcome group
Receptor activity and mechanism · Cardiovascular
Studied dose
Dose not stated
Source sentences
Summary
Binding was displaceable by oxytocin, vasopressin and the agonist [Thr4, Gly7] oxytocin but not by the antagonist atosiban.
1 context sentence(design, population, supporting numbers)
Summary · context
A radioligand binding assay was used to characterize and quantify specific binding of [3H] oxytocin to standardized micropunches (thickness 400 microm; diameter 2 mm) obtained from rat left ventricle and human right atrium.
Report a problemFND-1057974203
04LabEx vivo tissueActivity measurement
In rat heart tissue, oxytocin binding was reduced in ovariectomized rats.
In plain wordsIn rat heart tissue, oxytocin binding was lower in rats without ovaries.
Peptide
Oxytocin
Outcome
DecreaseCardiac oxytocin binding in ovariectomized rats
Study
ratn: not reported in source
Rat left ventricle from ovariectomized rats
Outcome group
Receptor activity and mechanism · Cardiovascular
Studied dose
Dose not stated
Source sentences
Summary
Cardiac binding was reduced in ovariectomized (ie, estrogen-free) rats and increased in late gestation rats, when blood levels of ovarian steroids are maximal.
1 context sentence(design, population, supporting numbers)
Summary · context
A radioligand binding assay was used to characterize and quantify specific binding of [3H] oxytocin to standardized micropunches (thickness 400 microm; diameter 2 mm) obtained from rat left ventricle and human right atrium.
Report a problemFND-1057974204
05LabEx vivo tissueActivity measurement
In rat heart tissue, oxytocin binding was increased in late gestation rats.
In plain wordsIn rat heart tissue, oxytocin binding was higher in rats late in pregnancy.
Peptide
Oxytocin
Outcome
IncreaseCardiac oxytocin binding in late gestation rats
Study
ratn: not reported in source
Rat left ventricle from late gestation rats
Outcome group
Receptor activity and mechanism · Cardiovascular
Studied dose
Dose not stated
Source sentences
Summary
and increased in late gestation rats, when blood levels of ovarian steroids are maximal.
1 context sentence(design, population, supporting numbers)
Summary · context
A radioligand binding assay was used to characterize and quantify specific binding of [3H] oxytocin to standardized micropunches (thickness 400 microm; diameter 2 mm) obtained from rat left ventricle and human right atrium.
Report a problemFND-1057974205
06LabEx vivo tissueActivity measurement
In fetal and newborn rat heart, cardiac oxytocin receptors were undetectable.
In plain wordsIn lab tests on fetal and newborn rat hearts, no oxytocin binding sites could be found.
Peptide
Oxytocin
Outcome
Direction not reportedOxytocin binding in fetal and newborn rat heart
Study
ratn: not reported in source
Fetal and newborn rat heart
Outcome group
Receptor activity and mechanism · Cardiovascular
Studied dose
Dose not stated
Source sentences
Summary
Cardiac oxytocin receptors were undetectable in fetal and newborn rat heart.
1 context sentence(design, population, supporting numbers)
Summary · context
A radioligand binding assay was used to characterize and quantify specific binding of [3H] oxytocin to standardized micropunches (thickness 400 microm; diameter 2 mm) obtained from rat left ventricle and human right atrium.
Report a problemFND-1057974206
07LabEx vivo tissueActivity measurement
In human right atrial appendage samples, high concentrations of specific [3H] oxytocin binding were found.
In plain wordsIn lab tests on human heart tissue, oxytocin bound at high levels to specific sites.
Peptide
Oxytocin
Outcome
Not applicableOxytocin binding in human right atrium
Study
humann: not reported in source
Human right atrial appendage
Outcome group
Receptor activity and mechanism · Cardiovascular
Studied dose
Dose not stated
Source sentences
Summary
High concentrations of specific [3H] oxytocin binding were also found in samples of human right atrial appendage.
1 context sentence(design, population, supporting numbers)
Summary · context
A radioligand binding assay was used to characterize and quantify specific binding of [3H] oxytocin to standardized micropunches (thickness 400 microm; diameter 2 mm) obtained from rat left ventricle and human right atrium.
Report a problemFND-1057974207

AI wrote and checked these results. AI can make mistakes, so check important results against the source.

Version history

PepBank keeps every version of the data on this study. Status changes from Retraction Watch, Europe PMC and Crossref are listed with their source.

Version 2Current
11 Oct 2026, 20:03 UTC
Study results updated
Version 1
9 Oct 2026, 02:51 UTC
First added · source update from Europe PMC

Subjects

Terms as the source lists them.

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MeSH terms

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