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Study · Not a test of the peptide alone · 1999

3 results

Expression, folding, and thermodynamic properties of the bovine oxytocin-neurophysin precursor: relationships to the intermolecular oxytocin-neurophysin complex.

Eubanks S, Lu M, Peyton D, Breslow E

Biochemistry1 Oct 1999Cited by 4 (Europe PMC)

PMID10521260 (opens in a new tab)DOI10.1021/bi9912950 (opens in a new tab)PepBankPUB-10521260
Publisher pagePubMedAsk about this study
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Summary

The text of this summary is not shown here, because the article license does not allow reuse. Read it at the source (opens in a new tab)

Text rights: Publisher copyright. Results still quote short attributed sentences.

Results from this study3

No result from this study comes from a test of the peptide.

Studies that did not test the peptide alone3

In this study, Oxytocin was a lab tool or a carrier, part of a mix, or the subject of a lab method. These results are not evidence about taking Oxytocin.

  1. 01LabNot a test of Oxytocin aloneIn vitro (cell-free)Mechanism

    In vitro, refolding of the bovine oxytocin-neurophysin precursor was as inefficient as refolding of reduced mature neurophysin with oxytocin present.

    In plain wordsIn lab tests, the oxytocin-neurophysin precursor refolded no better than mature neurophysin mixed with oxytocin.

    Peptide
    Oxytocin
    Outcome
    Not applicableRefolding efficiency of the precursor
    Study

    n: not reported in source

    Bovine oxytocin-neurophysin precursor expressed in E. coli and folded in vitro
    Outcome group
    Receptor activity and mechanism · Pharmacology

    Source sentences

    Summary
    The inefficiency associated with the refolding of reduced mature neurophysin in the presence of oxytocin was found not to be alleviated in the precursor.
    1 context sentence
    Summary · context
    To address this issue, the disulfide-rich precursor of oxytocin-associated bovine neurophysin was expressed in Escherichia coli and folded in vitro to yield milligram quantities of purified protein; evidence of significant impediments to yield resulting from damage to Cys residues is presented.

    In this study, Oxytocin was a lab tool or a carrier, part of a mix, or the subject of a lab method. This result is not evidence about taking Oxytocin.

  2. 02LabNot a test of Oxytocin aloneIn vitro (cell-free)Mechanism

    In vitro, noncovalent intramolecular bonding between oxytocin and neurophysin in the precursor had only a small thermodynamic advantage over the intermolecular complex.

    In plain wordsIn lab tests, oxytocin bound to neurophysin inside the precursor was only slightly more stable than in the separate complex.

    Peptide
    Oxytocin
    Outcome
    Not applicableThermodynamic advantage of intramolecular oxytocin-neurophysin bonding
    Study

    n: not reported in source

    Bovine oxytocin-neurophysin precursor expressed in E. coli and folded in vitro
    Outcome group
    Receptor activity and mechanism · Pharmacology

    Source sentences

    Summary
    Consistent with this, the effects of pH on the spectroscopic properties of the precursor and on the relative stabilities of the precursor and mature neurophysin to guanidine denaturation indicated that noncovalent intramolecular bonding between oxytocin and neurophysin in the precursor had only a sm…
    1 context sentence
    Summary · context
    To address this issue, the disulfide-rich precursor of oxytocin-associated bovine neurophysin was expressed in Escherichia coli and folded in vitro to yield milligram quantities of purified protein; evidence of significant impediments to yield resulting from damage to Cys residues is presented.

    In this study, Oxytocin was a lab tool or a carrier, part of a mix, or the subject of a lab method. This result is not evidence about taking Oxytocin.

  3. 03LabNot a test of Oxytocin aloneIn vitro (cell-free)Mechanism

    In vitro, loss of hormone-protein interactions and of the precursor's enhanced stability occurred reversibly with increasing pH, with a midpoint at pH 10.

    In plain wordsIn lab tests, the precursor's extra stability was lost reversibly as pH rose, with a midpoint at pH 10.

    Peptide
    Oxytocin
    Outcome
    Not applicablepH dependence of precursor stability
    Study

    n: not reported in source

    Bovine oxytocin-neurophysin precursor expressed in E. coli and folded in vitro
    Outcome group
    Receptor activity and mechanism · Pharmacology

    Measurements

    Transition midpoint
    10 pH
    oxytocin-neurophysin precursor

    Source sentences

    Summary
    Loss of the principal interactions between hormone and protein, and of the enhanced stability of the precursor relative to that of the mature unliganded protein, occurred reversibly upon increasing the pH, with a midpoint at pH 10.
    1 context sentence
    Summary · context
    To address this issue, the disulfide-rich precursor of oxytocin-associated bovine neurophysin was expressed in Escherichia coli and folded in vitro to yield milligram quantities of purified protein; evidence of significant impediments to yield resulting from damage to Cys residues is presented.

    In this study, Oxytocin was a lab tool or a carrier, part of a mix, or the subject of a lab method. This result is not evidence about taking Oxytocin.

AI wrote and checked these results. AI can make mistakes, so check important results against the source.

Version history

PepBank keeps every version of the data on this study. Status changes from Retraction Watch, Europe PMC and Crossref are listed with their source.

  1. Version 2Current

    11 Oct 2026, 20:03 UTC

    Study results updated

  2. Version 1

    9 Oct 2026, 02:51 UTC

    First added · source update from Europe PMC

Subjects

Terms as the source lists them.

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