The effect of GHRP-6 on the intracellular Na+ concentration of rat pituitary cells in primary culture.

Kato M, Sakuma Y

Journal of neuroendocrinology1 Oct 1999Cited by 11 (Europe PMC)

PMID10520128DOI10.1046/j.1365-2826.1999.00394.xPepBankPUB-10520128

Publisher page PubMedAsk about this study

Summary

The text of this summary is not shown here, because the article license does not allow reuse. Read it at the source (opens in a new tab)

Text rights: Publisher copyright. Results still quote short attributed sentences.

Results from this study8

2 results from studies that gave the peptide. No result from this study comes from people.

01LabIn vitro (cells)Mechanism
In cultured rat pituitary cells, GHRP‑6 increased intracellular sodium.
In plain wordsIn cells from rat pituitary glands, GHRP‑6 increased intracellular sodium.
Peptide
GHRP-6
Outcome
IncreaseIntracellular sodium
Study
ratn: not reported in source
male rat pituitary cells in primary culture
Outcome group
Receptor activity and mechanism · Endocrine and hormones
Studied dose
Dose not stated
Source sentences
Summary
These data clearly showed that GHRP-6 increased [Na+]i in the rat pituitary cells including somatotrophs.
1 context sentence(design, population, supporting numbers)
Summary · context
The objective of the present study was to further investigate the ionic mechanism of the action of GHRP-6 on male rat pituitary cells in culture.
Report a problemFND-1052012801
02LabIn vitro (cells)Mechanism
In cultured rat pituitary cells, the GHRP‑6 sodium response depended on extracellular sodium.
In plain wordsIn cells from rat pituitary glands, the sodium response to GHRP‑6 required sodium outside the cells.
Peptide
GHRP-6
Outcome
Not applicableExtracellular sodium dependence
Study
ratn: not reported in source
male rat pituitary cells in primary culture
Outcome group
Receptor activity and mechanism · Endocrine and hormones
Studied dose
Dose not stated
Source sentences
Summary
the Na+ response depended on the presence of extracellular Na+
1 context sentence(design, population, supporting numbers)
Summary · context
The objective of the present study was to further investigate the ionic mechanism of the action of GHRP-6 on male rat pituitary cells in culture.
Report a problemFND-1052012802

Studies that did not test the peptide alone6

In this study, GHRP-6 was a lab tool or a carrier, part of a mix, or the subject of a lab method. These results are not evidence about taking GHRP-6.

03LabNot a test of GHRP-6 aloneIn vitro (cells)Mechanism
In cultured rat pituitary cells, Gd3+ blocked the GHRP‑6 sodium response.
In plain wordsIn cells from rat pituitary glands, Gd3+ blocked the sodium response to GHRP‑6.
Peptide
GHRP-6
Outcome
DecreaseGadolinium blockade of sodium response
Study
ratn: not reported in source
male rat pituitary cells in primary culture
Outcome group
Receptor activity and mechanism · Endocrine and hormones
Source sentences
Summary
the Na+ response depended on the presence of extracellular Na+ and was blocked by Gd3+, known as a blocker of nonselective cation channels but not by tetrodotoxin, a blocker of the voltage-gated Na+ channel
1 context sentence(design, population, supporting numbers)
Summary · context
The objective of the present study was to further investigate the ionic mechanism of the action of GHRP-6 on male rat pituitary cells in culture.
In this study, GHRP-6 was a lab tool or a carrier, part of a mix, or the subject of a lab method. This result is not evidence about taking GHRP-6.
Report a problemFND-1052012803
04LabNot a test of GHRP-6 aloneIn vitro (cells)Mechanism
In cultured rat pituitary cells, tetrodotoxin did not block the GHRP‑6 sodium response.
In plain wordsIn cells from rat pituitary glands, tetrodotoxin did not block the sodium response to GHRP‑6.
Peptide
GHRP-6
Outcome
No significant changeTetrodotoxin effect on sodium response
Study
ratn: not reported in source
male rat pituitary cells in primary culture
Outcome group
Receptor activity and mechanism · Endocrine and hormones
Source sentences
Summary
the Na+ response depended on the presence of extracellular Na+ and was blocked by Gd3+, known as a blocker of nonselective cation channels but not by tetrodotoxin, a blocker of the voltage-gated Na+ channel
1 context sentence(design, population, supporting numbers)
Summary · context
The objective of the present study was to further investigate the ionic mechanism of the action of GHRP-6 on male rat pituitary cells in culture.
In this study, GHRP-6 was a lab tool or a carrier, part of a mix, or the subject of a lab method. This result is not evidence about taking GHRP-6.
Report a problemFND-1052012804
05LabNot a test of GHRP-6 aloneIn vitro (cells)Mechanism
In cultured rat pituitary cells, thapsigargin did not affect the GHRP‑6 sodium response.
In plain wordsIn cells from rat pituitary glands, thapsigargin did not change the sodium response to GHRP‑6.
Peptide
GHRP-6
Outcome
No significant changeThapsigargin effect on sodium response
Study
ratn: not reported in source
male rat pituitary cells in primary culture
Outcome group
Receptor activity and mechanism · Endocrine and hormones
Source sentences
Summary
thapsigargin, an inhibitor of endoplasmic reticulum Ca2+ ATPase, had no effect on the response
1 context sentence(design, population, supporting numbers)
Summary · context
The objective of the present study was to further investigate the ionic mechanism of the action of GHRP-6 on male rat pituitary cells in culture.
In this study, GHRP-6 was a lab tool or a carrier, part of a mix, or the subject of a lab method. This result is not evidence about taking GHRP-6.
Report a problemFND-1052012805
06LabNot a test of GHRP-6 aloneIn vitro (cells)Mechanism
In cultured rat pituitary cells, BAPTA did not inhibit the GHRP‑6 sodium response.
In plain wordsIn cells from rat pituitary glands, BAPTA did not inhibit the sodium response to GHRP‑6.
Peptide
GHRP-6
Outcome
No significant changeBAPTA effect on sodium response
Study
ratn: not reported in source
male rat pituitary cells in primary culture
Outcome group
Receptor activity and mechanism · Endocrine and hormones
Source sentences
Summary
Ca2+ chelating agent, BAPTA had no inhibitory effect on the response
1 context sentence(design, population, supporting numbers)
Summary · context
The objective of the present study was to further investigate the ionic mechanism of the action of GHRP-6 on male rat pituitary cells in culture.
In this study, GHRP-6 was a lab tool or a carrier, part of a mix, or the subject of a lab method. This result is not evidence about taking GHRP-6.
Report a problemFND-1052012806
07LabNot a test of GHRP-6 aloneIn vitro (cells)Mechanism
In cultured rat pituitary cells, ouabain did not block the GHRP‑6-induced sodium rise.
In plain wordsIn cells from rat pituitary glands, ouabain did not block the sodium increase from GHRP‑6.
Peptide
GHRP-6
Outcome
No significant changeOuabain effect on sodium response
Study
ratn: not reported in source
male rat pituitary cells in primary culture
Outcome group
Receptor activity and mechanism · Endocrine and hormones
Source sentences
Summary
ouabain, an inhibitor of Na+-K+ ATPase, did not block the rise in [Na+]i induced by GHRP-6
1 context sentence(design, population, supporting numbers)
Summary · context
The objective of the present study was to further investigate the ionic mechanism of the action of GHRP-6 on male rat pituitary cells in culture.
In this study, GHRP-6 was a lab tool or a carrier, part of a mix, or the subject of a lab method. This result is not evidence about taking GHRP-6.
Report a problemFND-1052012807
08LabNot a test of GHRP-6 aloneIn vitro (cells)Mechanism
In cultured rat pituitary cells, somatostatin suppressed the GHRP‑6 sodium response.
In plain wordsIn cells from rat pituitary glands, somatostatin suppressed the sodium response to GHRP‑6.
Peptide
GHRP-6
Outcome
DecreaseSomatostatin effect on sodium response
Study
ratn: not reported in source
male rat pituitary cells in primary culture
Outcome group
Receptor activity and mechanism · Endocrine and hormones
Source sentences
Summary
somatostatin, which hyperpolarizes the cells by activating K+ channels, suppressed the response.
1 context sentence(design, population, supporting numbers)
Summary · context
The objective of the present study was to further investigate the ionic mechanism of the action of GHRP-6 on male rat pituitary cells in culture.
In this study, GHRP-6 was a lab tool or a carrier, part of a mix, or the subject of a lab method. This result is not evidence about taking GHRP-6.
Report a problemFND-1052012808

AI wrote and checked these results. AI can make mistakes, so check important results against the source.

Version history

PepBank keeps every version of the data on this study. Status changes from Retraction Watch, Europe PMC and Crossref are listed with their source.

Version 2Current
21 Oct 2026, 15:58 UTC
Study results updated
Version 1
9 Oct 2026, 03:13 UTC
First added · source update from Europe PMC

Subjects

Terms as the source lists them.

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