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Study · Not a test of the peptide alone · 1999

5 results

Immobilization of tripeptide growth factor glycyl-L-histidyl-L-lysine on poly(vinylalcohol)-quarternized stilbazole (PVA-SbQ) and its use as a ligand for hepatocyte attachment.

Kawase M, Miura N, Kurikawa N, Masuda K, Higashiyama S, Yagi K, Mizoguchi T

Biological & pharmaceutical bulletin1 Sept 1999Cited by 6 (Europe PMC)

PMID10513632 (opens in a new tab)DOI10.1248/bpb.22.999 (opens in a new tab)PepBankPUB-10513632
Publisher pagePubMedAsk about this study
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Summary

The text of this summary is not shown here, because the article license does not allow reuse. Read it at the source (opens in a new tab)

Text rights: Publisher copyright. Results still quote short attributed sentences.

Results from this study5

No result from this study comes from a test of the peptide.

Studies that did not test the peptide alone5

In this study, GHK (glycyl-L-histidyl-L-lysine) was a lab tool or a carrier, part of a mix, or the subject of a lab method. These results are not evidence about taking GHK (glycyl-L-histidyl-L-lysine).

  1. 01LabNot a test of GHK (glycyl-L-histidyl-L-lysine) aloneIn vitro (cells)Activity measurement

    In isolated rat hepatocytes on GHK-immobilized gel, aggregation and multicellular spheroid formation began about 24 h after inoculation.

    In plain wordsIn cells from rat liver, GHK-immobilized gel supported aggregation and spheroid formation about 24 h after inoculation.

    Peptide
    GHK (glycyl-L-histidyl-L-lysine)
    Outcome
    IncreaseHepatocyte aggregation
    Study

    ratn: not reported in source

    isolated rat hepatocytes on GHK-immobilized PVA-SbQ gel
    Outcome group
    Receptor activity and mechanism · Liver

    Source sentences

    Summary
    About 24 h after inoculation, hepatocytes started to aggregate and formed multicellular spheroids while almost no cells attached to GHK-non-immobilized PVA-SbQ gel.
    1 context sentence
    Summary · context
    Isolated rat hepatocytes were inoculated on the GHK-immobilized PVA-SbQ gel and cultured for 5 d.

    In this study, GHK (glycyl-L-histidyl-L-lysine) was a lab tool or a carrier, part of a mix, or the subject of a lab method. This result is not evidence about taking GHK (glycyl-L-histidyl-L-lysine).

  2. 02LabNot a test of GHK (glycyl-L-histidyl-L-lysine) aloneIn vitro (cells)Activity measurement

    In isolated rat hepatocytes, GHK-immobilized gel supported attachment, while almost no cells attached to gel without immobilized GHK.

    In plain wordsIn cells from rat liver, attachment occurred on GHK-immobilized gel, while almost none occurred on gel without GHK.

    Peptide
    GHK (glycyl-L-histidyl-L-lysine)
    Outcome
    IncreaseHepatocyte attachment
    Study

    ratn: not reported in source

    isolated rat hepatocytes on GHK-immobilized PVA-SbQ gel
    Comparator
    GHK-non-immobilized PVA-SbQ gel
    Outcome group
    Receptor activity and mechanism · Liver

    Source sentences

    Summary
    About 24 h after inoculation, hepatocytes started to aggregate and formed multicellular spheroids while almost no cells attached to GHK-non-immobilized PVA-SbQ gel.
    2 context sentences
    Summary · context
    The formed spheroids attached firmly to the surface of PVA-SbQ gel for 5 d.
    Summary · context
    Isolated rat hepatocytes were inoculated on the GHK-immobilized PVA-SbQ gel and cultured for 5 d.

    In this study, GHK (glycyl-L-histidyl-L-lysine) was a lab tool or a carrier, part of a mix, or the subject of a lab method. This result is not evidence about taking GHK (glycyl-L-histidyl-L-lysine).

  3. 03LabNot a test of GHK (glycyl-L-histidyl-L-lysine) aloneIn vitro (cells)Activity measurement

    In isolated rat hepatocytes, spheroids remained firmly attached to GHK-immobilized PVA-SbQ gel for 5 d.

    In plain wordsIn cells from rat liver, spheroids remained attached to GHK-immobilized gel for 5 d.

    Peptide
    GHK (glycyl-L-histidyl-L-lysine)
    Outcome
    Not applicableSpheroid attachment persistence
    Study

    ratn: not reported in source

    isolated rat hepatocytes on GHK-immobilized PVA-SbQ gel
    Outcome group
    Receptor activity and mechanism · Liver

    Source sentences

    Summary
    The formed spheroids attached firmly to the surface of PVA-SbQ gel for 5 d.
    1 context sentence
    Summary · context
    Isolated rat hepatocytes were inoculated on the GHK-immobilized PVA-SbQ gel and cultured for 5 d.

    In this study, GHK (glycyl-L-histidyl-L-lysine) was a lab tool or a carrier, part of a mix, or the subject of a lab method. This result is not evidence about taking GHK (glycyl-L-histidyl-L-lysine).

  4. 04LabNot a test of GHK (glycyl-L-histidyl-L-lysine) aloneIn vitro (cells)Activity measurement

    In isolated rat hepatocytes, extending the gel-GHK linkage with dodecamethylenediamine significantly increased attached cell numbers.

    In plain wordsIn cells from rat liver, extending the gel-GHK linkage increased attachment compared with the unextended linkage.

    Peptide
    GHK (glycyl-L-histidyl-L-lysine)
    Outcome
    IncreaseAttachment after spacer extension
    Study

    ratn: not reported in source

    isolated rat hepatocytes on GHK-immobilized PVA-SbQ gel
    Comparator
    Unextended gel-GHK linkage
    Outcome group
    Receptor activity and mechanism · Liver

    Source sentences

    Summary
    Extension of the length significantly increased the number of attached hepatocytes.
    2 context sentences
    Summary · context
    Dodecamethylenediamine was used to extend the length between the gel surface and GHK.
    Summary · context
    Isolated rat hepatocytes were inoculated on the GHK-immobilized PVA-SbQ gel and cultured for 5 d.

    In this study, GHK (glycyl-L-histidyl-L-lysine) was a lab tool or a carrier, part of a mix, or the subject of a lab method. This result is not evidence about taking GHK (glycyl-L-histidyl-L-lysine).

  5. 05LabNot a test of GHK (glycyl-L-histidyl-L-lysine) aloneIn vitro (cell-free)Activity measurement

    On PVA-SbQ gel, immobilized GHK density was 70 nmol/cm2.

    In plain wordsIn lab tests, the gel carried GHK at 70 nmol/cm2.

    Peptide
    GHK (glycyl-L-histidyl-L-lysine)
    Outcome
    Not applicableImmobilized GHK density
    Study

    n: not reported in source

    GHK-immobilized PVA-SbQ gel
    Outcome group
    Receptor activity and mechanism · Liver

    Measurements

    GHK density
    70 nmol/cm2
    PVA-SbQ gel

    Source sentences

    Summary
    The density of immobilized GHK was 70 nmol/cm2.

    In this study, GHK (glycyl-L-histidyl-L-lysine) was a lab tool or a carrier, part of a mix, or the subject of a lab method. This result is not evidence about taking GHK (glycyl-L-histidyl-L-lysine).

AI wrote and checked these results. AI can make mistakes, so check important results against the source.

Version history

PepBank keeps every version of the data on this study. Status changes from Retraction Watch, Europe PMC and Crossref are listed with their source.

  1. Version 2Current

    14 Oct 2026, 05:02 UTC

    Study results updated

  2. Version 1

    14 Oct 2026, 01:46 UTC

    First added · source update from Europe PMC

Subjects

Terms as the source lists them.

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