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Study · Not a test of the peptide alone · 1999

9 results

Type I beta-turn conformation is important for biological activity of the melanocyte-stimulating hormone analogues.

Li SZ, Lee JH, Lee W, Yoon CJ, Baik JH, Lim SK

European journal of biochemistry1 Oct 1999Cited by 29 (Europe PMC)

PMID10491201 (opens in a new tab)DOI10.1046/j.1432-1327.1999.00763.x (opens in a new tab)PepBankPUB-10491201
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Summary

The text of this summary is not shown here, because the article license does not allow reuse. Read it at the source (opens in a new tab)

Text rights: Publisher copyright. Results still quote short attributed sentences.

Results from this study9

No result from this study comes from a test of the peptide.

Studies that did not test the peptide alone9

In this study, Melanotan II was a lab tool or a carrier, part of a mix, or the subject of a lab method. These results are not evidence about taking Melanotan II.

  1. 01LabNot a test of Melanotan II aloneIn vitro (cells)Activity measurement

    Linear MTII (alpha-MSH-ND) preferred the MC4R, with IC50 and EC50 values comparable to those reported earlier for MTII.

    In plain wordsIn cells, a linear form of MTII preferred one melanocortin receptor, with potency like earlier MTII reports.

    Peptide
    Melanotan II
    Outcome
    Not applicableReceptor preference of linear MTII (alpha-MSH-ND)
    Study

    n: not reported in source

    Outcome group
    Receptor activity and mechanism · Pharmacology

    Source sentences

    Summary
    Compared with [Ahx4]alpha-MSH, the linear MTII designated as alpha-MSH-ND revealed a preference for the MC4R, whereas its IC50 and EC50 values were comparable to those of MTII reported previously.
    1 context sentence
    Summary · context
    In order to define which structure of alpha-melanocyte-stimulating hormone (MSH) analogues plays a critical role for ligand-receptor interaction and selectivity, we analysed receptor-binding and cAMP-generating activity in Chinese hamster ovary cell lines stably transfected with rMC3R and hMC4R, as…

    In this study, Melanotan II was a lab tool or a carrier, part of a mix, or the subject of a lab method. This result is not evidence about taking Melanotan II.

  2. 02LabNot a test of Melanotan II aloneIn vitro (cells)Activity measurement

    Truncating Ahx4 and Asp5 of alpha-MSH-ND remarkably decreased receptor binding and cAMP-generating activity.

    In plain wordsIn cells, shortening the MTII-like peptide at one end strongly reduced its receptor binding and activity.

    Peptide
    Melanotan II
    Outcome
    DecreaseEffect of Ahx4 and Asp5 truncation on binding and cAMP activity
    Study

    n: not reported in source

    Outcome group
    Receptor activity and mechanism · Pharmacology

    Source sentences

    Summary
    Truncation of Ahx4 and Asp5 of alpha-MSH-ND remarkably decreased the receptor-binding and cAMP-generating activity.
    1 context sentence
    Summary · context
    In order to define which structure of alpha-melanocyte-stimulating hormone (MSH) analogues plays a critical role for ligand-receptor interaction and selectivity, we analysed receptor-binding and cAMP-generating activity in Chinese hamster ovary cell lines stably transfected with rMC3R and hMC4R, as…

    In this study, Melanotan II was a lab tool or a carrier, part of a mix, or the subject of a lab method. This result is not evidence about taking Melanotan II.

  3. 03LabNot a test of Melanotan II aloneIn vitro (cells)Activity measurement

    Alpha-MSH-ND(6-10) needed a higher concentration for maximum cAMP-generating activity, and its preference changed from MC4R to MC3R.

    In plain wordsIn cells, a shortened MTII-like peptide needed a higher dose for full activity and switched receptor preference.

    Peptide
    Melanotan II
    Outcome
    MixedcAMP activity and receptor preference of alpha-MSH-ND(6-10)
    Study

    n: not reported in source

    Outcome group
    Receptor activity and mechanism · Pharmacology

    Source sentences

    Summary
    Meanwhile, maximum cAMP-generating activity was observed at a higher concentration (10(-5) M) of alpha-MSH-ND(6-10), and MC4R preference was changed into MC3R preference.
    1 context sentence
    Summary · context
    In order to define which structure of alpha-melanocyte-stimulating hormone (MSH) analogues plays a critical role for ligand-receptor interaction and selectivity, we analysed receptor-binding and cAMP-generating activity in Chinese hamster ovary cell lines stably transfected with rMC3R and hMC4R, as…

    In this study, Melanotan II was a lab tool or a carrier, part of a mix, or the subject of a lab method. This result is not evidence about taking Melanotan II.

  4. 04LabNot a test of Melanotan II aloneIn vitro (cells)Activity measurement

    [Gln6]alpha-MSH-ND(6-10) bound both receptors but almost completely lost cAMP-generating activity.

    In plain wordsIn cells, this altered peptide still bound the receptors but almost lost its activity.

    Peptide
    Melanotan II
    Outcome
    MixedcAMP activity of [Gln6]alpha-MSH-ND(6-10)
    Study

    n: not reported in source

    Outcome group
    Receptor activity and mechanism · Pharmacology

    Source sentences

    Summary
    In contrast, [Gln6]alpha-MSH-ND(6-10) lost its cAMP-generating activity almost completely, even though it bound to both receptors.
    1 context sentence
    Summary · context
    In order to define which structure of alpha-melanocyte-stimulating hormone (MSH) analogues plays a critical role for ligand-receptor interaction and selectivity, we analysed receptor-binding and cAMP-generating activity in Chinese hamster ovary cell lines stably transfected with rMC3R and hMC4R, as…

    In this study, Melanotan II was a lab tool or a carrier, part of a mix, or the subject of a lab method. This result is not evidence about taking Melanotan II.

  5. 05LabNot a test of Melanotan II aloneIn vitro (cells)Activity measurement

    Replacing His6 of alpha-MSH-ND by Gln, Asn, Arg or Lys decreased receptor binding and cAMP-generating activity at both MC3R and MC4R.

    In plain wordsIn cells, swapping one amino acid in the MTII-like peptide reduced its binding and activity at two receptors.

    Peptide
    Melanotan II
    Outcome
    DecreaseEffect of His6 replacement on binding and cAMP activity
    Study

    n: not reported in source

    Outcome group
    Receptor activity and mechanism · Pharmacology

    Source sentences

    Summary
    Replacement of the His6 residue of alpha-MSH-ND by Gln, Asn, Arg or Lys decreased not only the receptor binding, but also the cAMP-generating activity in both the MC3R and the MC4R.
    1 context sentence
    Summary · context
    In order to define which structure of alpha-melanocyte-stimulating hormone (MSH) analogues plays a critical role for ligand-receptor interaction and selectivity, we analysed receptor-binding and cAMP-generating activity in Chinese hamster ovary cell lines stably transfected with rMC3R and hMC4R, as…

    In this study, Melanotan II was a lab tool or a carrier, part of a mix, or the subject of a lab method. This result is not evidence about taking Melanotan II.

  6. 06LabNot a test of Melanotan II aloneIn vitro (cells)Activity measurement

    [Lys6]alpha-MSH-ND showed greatly reduced binding affinity and cAMP-generating activity and lost MC4R selectivity.

    In plain wordsIn cells, the Lys-substituted peptide bound and activated receptors much less and lost its receptor preference.

    Peptide
    Melanotan II
    Outcome
    Decrease[Lys6]alpha-MSH-ND binding, activity and selectivity
    Study

    n: not reported in source

    Outcome group
    Receptor activity and mechanism · Pharmacology

    Source sentences

    Summary
    [Lys6]alpha-MSH-ND showed a greatly reduced binding affinity and cAMP-generating activity with the loss of MC4R selectivity.
    1 context sentence
    Summary · context
    In order to define which structure of alpha-melanocyte-stimulating hormone (MSH) analogues plays a critical role for ligand-receptor interaction and selectivity, we analysed receptor-binding and cAMP-generating activity in Chinese hamster ovary cell lines stably transfected with rMC3R and hMC4R, as…

    In this study, Melanotan II was a lab tool or a carrier, part of a mix, or the subject of a lab method. This result is not evidence about taking Melanotan II.

  7. 07LabNot a test of Melanotan II aloneIn vitro (cell-free)Mechanism

    Alpha-MSH-ND had a stable type I beta-turn structure, whereas [Gln6]alpha-MSH-ND(6-10) had a tight gamma-turn.

    In plain wordsIn lab tests, the MTII-like peptide had a stable turn shape that its inactive analog lacked.

    Peptide
    Melanotan II
    Outcome
    Not applicableSolution conformation of alpha-MSH-ND and [Gln6]alpha-MSH-ND(6-10)
    Study

    n: not reported in source

    Outcome group
    Receptor activity and mechanism · Pharmacology

    Source sentences

    Summary
    Whereas the solution conformation of alpha-MSH-ND revealed a stable type I beta-turn structure, [Gln6]alpha-MSH-ND(6-10) revealed a tight gamma-turn composed of Gln6-D-Phe7-Arg8.

    In this study, Melanotan II was a lab tool or a carrier, part of a mix, or the subject of a lab method. This result is not evidence about taking Melanotan II.

  8. 08LabNot a test of Melanotan II aloneIn vitro (cell-free)Mechanism

    In NMR studies, [Lys6]alpha-MSH-ND showed a gamma-turn conformation around Lys6-DPhe7-Arg8.

    In plain wordsIn lab tests, the Lys-substituted peptide had a different turn shape from the active peptide.

    Peptide
    Melanotan II
    Outcome
    Not applicableGamma-turn conformation of [Lys6]alpha-MSH-ND
    Study

    n: not reported in source

    Outcome group
    Receptor activity and mechanism · Pharmacology

    Source sentences

    Summary
    In NMR studies, [Lys6]alpha-MSH-ND also demonstrated a gamma-turn conformation around Lys6-DPhe7-Arg8.

    In this study, Melanotan II was a lab tool or a carrier, part of a mix, or the subject of a lab method. This result is not evidence about taking Melanotan II.

  9. 09LabNot a test of Melanotan II aloneIn vitro (cell-free)Mechanism

    The structure of [Gln6]alpha-MSH-ND showed a stable type I' beta-turn comprising Asp5, Gln6, D-Phe7 and Arg8.

    In plain wordsIn lab tests, the Gln-substituted MTII-like peptide had a stable turn shape in its structure.

    Peptide
    Melanotan II
    Outcome
    Not applicableStructure of [Gln6]alpha-MSH-ND
    Study

    n: not reported in source

    Outcome group
    Receptor activity and mechanism · Pharmacology

    Source sentences

    Summary
    The structure of [Gln6]alpha-MSH-ND exhibited a stable type I' beta-turn comprising Asp5, Gln6, D-Phe7 and Arg8.

    In this study, Melanotan II was a lab tool or a carrier, part of a mix, or the subject of a lab method. This result is not evidence about taking Melanotan II.

AI wrote and checked these results. AI can make mistakes, so check important results against the source.

Version history

PepBank keeps every version of the data on this study. Status changes from Retraction Watch, Europe PMC and Crossref are listed with their source.

  1. Version 2Current

    10 Oct 2026, 09:43 UTC

    Study results updated

  2. Version 1

    9 Oct 2026, 03:04 UTC

    First added · source update from Europe PMC

Subjects

Terms as the source lists them.

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