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Study · In vitro (cells) · rat · 1999

2 results

Effect of estrogen agonists and antagonists on induction of progesterone receptor in a rat hypothalamic cell line.

Fitzpatrick SL, Berrodin TJ, Jenkins SF, Sindoni DM, Deecher DC, Frail DE

Endocrinology1 Sept 1999Cited by 27 (Europe PMC)

PMID10465261 (opens in a new tab)DOI10.1210/endo.140.9.7006 (opens in a new tab)PepBankPUB-10465261
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Summary

The text of this summary is not shown here, because the article license does not allow reuse. Read it at the source (opens in a new tab)

Text rights: Publisher copyright. Results still quote short attributed sentences.

Results from this study2

2 results from studies that gave the peptide. No result from this study comes from people.

  1. 01LabIn vitro (cells)Activity measurement

    In rat D12 hypothalamic cells, enclomiphene inhibited estradiol-induced progesterone receptor induction, with an IC50 of 77 nM.

    In plain wordsIn cells from rat hypothalamus, enclomiphene reduced progesterone receptor induction caused by estradiol.

    Peptide
    Enclomiphene
    Outcome
    DecreaseEstradiol-induced progesterone receptor induction
    Study

    ratn: not reported in source

    D12 embryonic hypothalamic cells
    Comparator
    estradiol stimulation
    Outcome group
    Receptor activity and mechanism · Endocrine and hormones
    Studied dose
    Dose not stated

    Measurements

    IC50
    77 nM
    enclomiphene inhibition of PR induction

    Source sentences

    Summary
    ICI 182,780 (IC50 = 0.63 nM), raloxifene (IC50 = 1 nM), enclomiphene (IC50 = 77 nM), and tamoxifen (IC50 = 174 nM) inhibited the induction of PR by estradiol
    2 context sentences
    Summary · context
    To understand the molecular mechanism(s) of estrogen action in the hypothalamus, immortalized rat embryonic hypothalamic cell lines were characterized for steroid receptors and subcloned.
    Summary · context
    Because the agonist or antagonist activity of estrogen mimetics can be cell type dependent, the activities of various estrogen mimetics were determined in D12 cells.
  2. 02LabIn vitro (cells)Activity measurement

    In rat D12 hypothalamic cells, enclomiphene alone did not significantly stimulate progesterone receptors.

    In plain wordsIn cells from rat hypothalamus, enclomiphene alone did not significantly stimulate progesterone receptors.

    Peptide
    Enclomiphene
    Outcome
    No significant changeProgesterone receptor stimulation alone
    Study

    ratn: not reported in source

    D12 embryonic hypothalamic cells
    Outcome group
    Receptor activity and mechanism · Endocrine and hormones
    Studied dose
    Dose not stated

    Source sentences

    Summary
    ICI 182,780 (IC50 = 0.63 nM), raloxifene (IC50 = 1 nM), enclomiphene (IC50 = 77 nM), and tamoxifen (IC50 = 174 nM) inhibited the induction of PR by estradiol, and none of these compounds significantly stimulated PR when given alone.
    2 context sentences
    Summary · context
    To understand the molecular mechanism(s) of estrogen action in the hypothalamus, immortalized rat embryonic hypothalamic cell lines were characterized for steroid receptors and subcloned.
    Summary · context
    Because the agonist or antagonist activity of estrogen mimetics can be cell type dependent, the activities of various estrogen mimetics were determined in D12 cells.

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Version history

PepBank keeps every version of the data on this study. Status changes from Retraction Watch, Europe PMC and Crossref are listed with their source.

  1. Version 2Current

    21 Oct 2026, 15:58 UTC

    Study results updated

  2. Version 1

    21 Oct 2026, 12:50 UTC

    First added · source update from Europe PMC

Subjects

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