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Study · Ex vivo tissue · 1999

5 results

Thymosin-alpha1 stimulates maturation of CD34+ stem cells into CD3+4+ cells in an in vitro thymic epithelia organ coculture model.

Knutsen AP, Freeman JJ, Mueller KR, Roodman ST, Bouhasin JD

International journal of immunopharmacology1 Jan 1999Cited by 59 (Europe PMC)

PMID10411279 (opens in a new tab)DOI10.1016/s0192-0561(98)00060-5 (opens in a new tab)PepBankPUB-10411279
Publisher pagePubMedAsk about this study
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Summary

The text of this summary is not shown here, because the article license does not allow reuse. Read it at the source (opens in a new tab)

Text rights: Publisher copyright. Results still quote short attributed sentences.

Results from this study5

5 results from studies that gave the peptide. No result from this study comes from people.

  1. 01LabEx vivo tissueActivity measurement

    In a human stem cell and thymic epithelial fragment coculture, thymosin-alpha1 increased the mononuclear cell number at 2 and 4 weeks.

    In plain wordsIn lab tests with human stem cells, thymosin alpha‑1 increased the number of cells grown in coculture.

    Peptide
    Thymosin alpha-1
    Outcome
    IncreaseMononuclear cell number in stem cell-thymic epithelia coculture
    Study

    humann: not reported in source

    CD34+ stem cells cocultured with allogeneic cultured thymic epithelial fragments
    Outcome group
    Immune response · Immune and inflammation
    Studied dose
    Dose not stated · 1-4 weeksAs given in this study (human).

    Source sentences

    Summary
    Thymosin-alpha1 significantly enhanced the cell number (e.g., proliferation) of mononuclear cells obtained at 2 and 4 weeks of the SC-CTEF cocultures (P < 0.01 and < 0.05, respectively).
    1 context sentence
    Summary · context
    In this study, the effect of thymosin-alpha1 on thymopoiesis was determined by coculturing in vitro CD34+ stem cells (SC) with allogeneic cultured thymic epithelia fragments (CTEF) for 1-4 weeks and analyzing T-cell maturation by flow cytometry.
  2. 02LabEx vivo tissueActivity measurement

    In the coculture, thymosin-alpha1 increased expression of CD3+ cells at 3 and 4 weeks.

    In plain wordsIn lab tests with human stem cells, thymosin alpha‑1 increased the number of CD3-positive T-cell marker cells.

    Peptide
    Thymosin alpha-1
    Outcome
    IncreaseCD3+ cell expression at weeks 3 and 4
    Study

    humann: not reported in source

    CD34+ stem cells cocultured with allogeneic cultured thymic epithelial fragments
    Outcome group
    Immune response · Immune and inflammation
    Studied dose
    Dose not stated · 1-4 weeksAs given in this study (human).

    Source sentences

    Summary
    In particular, thymosin-alpha1 stimulated expression of CD3+ cells at 3 and 4 weeks (P < 0.05).
    1 context sentence
    Summary · context
    In this study, the effect of thymosin-alpha1 on thymopoiesis was determined by coculturing in vitro CD34+ stem cells (SC) with allogeneic cultured thymic epithelia fragments (CTEF) for 1-4 weeks and analyzing T-cell maturation by flow cytometry.
  3. 03LabEx vivo tissueActivity measurement

    In the coculture, the predominant subpopulation increased by thymosin stimulation was single positive mature CD4+ cells.

    In plain wordsIn lab tests with human stem cells, thymosin alpha‑1 mainly increased mature CD4-positive T cells.

    Peptide
    Thymosin alpha-1
    Outcome
    IncreaseSingle positive mature CD4+ cells
    Study

    humann: not reported in source

    CD34+ stem cells cocultured with allogeneic cultured thymic epithelial fragments
    Outcome group
    Immune response · Immune and inflammation
    Studied dose
    Dose not stated · 1-4 weeksAs given in this study (human).

    Source sentences

    Summary
    The predominant subpopulation increased by thymosin stimulation was single positive mature CD4+ cells, which was confirmed to occur within the SC-CTEF thymic organ tissue by laser confocal immunofluorescence microscopy.
    1 context sentence
    Summary · context
    In this study, the effect of thymosin-alpha1 on thymopoiesis was determined by coculturing in vitro CD34+ stem cells (SC) with allogeneic cultured thymic epithelia fragments (CTEF) for 1-4 weeks and analyzing T-cell maturation by flow cytometry.
  4. 04LabEx vivo tissueMechanism

    In the coculture, thymosin stimulation tended to enhance IL-7 synthesis; the abstract describes a tendency, not a significant effect.

    In plain wordsIn lab tests with human stem cells, thymosin alpha‑1 showed only a trend toward more IL-7 production, not a clear effect.

    Peptide
    Thymosin alpha-1
    Outcome
    No significant changeIL-7 synthesis
    Study

    humann: not reported in source

    CD34+ stem cells cocultured with allogeneic cultured thymic epithelial fragments
    Outcome group
    Immune response · Immune and inflammation
    Studied dose
    Dose not stated · 1-4 weeksAs given in this study (human).

    Source sentences

    Summary
    Thymosin stimulation tended to enhance IL-7 synthesis, critical cytokine in the maturation of thymocytes.
    1 context sentence
    Summary · context
    In this study, the effect of thymosin-alpha1 on thymopoiesis was determined by coculturing in vitro CD34+ stem cells (SC) with allogeneic cultured thymic epithelia fragments (CTEF) for 1-4 weeks and analyzing T-cell maturation by flow cytometry.
  5. 05LabEx vivo tissueActivity measurement

    In the coculture, thymosin significantly increased expression of CD3+4+ T cells.

    In plain wordsIn lab tests with human stem cells, thymosin alpha‑1 significantly increased CD3+4+ T cells.

    Peptide
    Thymosin alpha-1
    Outcome
    IncreaseCD3+4+ T cell expression
    Study

    humann: not reported in source

    CD34+ stem cells cocultured with allogeneic cultured thymic epithelial fragments
    Outcome group
    Immune response · Immune and inflammation
    Studied dose
    Dose not stated · 1-4 weeksAs given in this study (human).

    Source sentences

    Summary
    Furthermore, the thymosin significantly increased expression of CD3+4+ T cells.
    1 context sentence
    Summary · context
    In this study, the effect of thymosin-alpha1 on thymopoiesis was determined by coculturing in vitro CD34+ stem cells (SC) with allogeneic cultured thymic epithelia fragments (CTEF) for 1-4 weeks and analyzing T-cell maturation by flow cytometry.

AI wrote and checked these results. AI can make mistakes, so check important results against the source.

Version history

PepBank keeps every version of the data on this study. Status changes from Retraction Watch, Europe PMC and Crossref are listed with their source.

  1. Version 3Current

    10 Oct 2026, 08:55 UTC

    Study results updated

  2. Version 2

    9 Oct 2026, 03:03 UTC

    Study data updated · source update from Europe PMC

  3. Version 1

    9 Oct 2026, 03:02 UTC

    First added · source update from Europe PMC

Subjects

Terms as the source lists them.

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