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Study · Not a test of the peptide alone · 1998

6 results

Chemical treatment of Escherichia coli. II. Direct extraction of recombinant protein from cytoplasmic inclusion bodies in intact cells.

Falconer RJ, O'Neill BK, Middelberg AP

Biotechnology and bioengineering1 Feb 1998Cited by 14 (Europe PMC)

PMID10099214 (opens in a new tab)DOI10.1002/(sici)1097-0290(19980220)57:4<381::aid-bit1>3.0.co;2-i (opens in a new tab)PepBankPUB-10099214
Publisher pagePubMedAsk about this study
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Summary

The text of this summary is not shown here, because the article license does not allow reuse. Read it at the source (opens in a new tab)

Text rights: Publisher copyright. Results still quote short attributed sentences.

Results from this study6

No result from this study comes from a test of the peptide.

Studies that did not test the peptide alone6

In this study, IGF-1 LR3 was a lab tool or a carrier, part of a mix, or the subject of a lab method. These results are not evidence about taking IGF-1 LR3.

  1. 01LabNot a test of IGF-1 LR3 aloneOther designOther

    Treatment of intact E. coli cells with 6M urea, 3 mM EDTA and 20 mM dithiothreitol at pH 9.0 extracted recombinant Long-R3-IGF-I effectively.

    In plain wordsIn bacteria, a chemical mix pulled out recombinant Long-R3-IGF-I effectively.

    Peptide
    IGF-1 LR3
    Outcome
    Not applicableDirect extraction of Long-R3-IGF-I from intact cells
    Study

    Escherichia colin: not reported in source

    intact E. coli cells with cytoplasmic inclusion bodies
    Outcome group
    Synthesis and manufacturing · Pharmacology

    Source sentences

    Summary
    Chemical treatment with 6M urea, 3 mM EDTA, and 20 mM dithiothreitol (DTT) at pH 9.0 proved an effective combination for extracting recombinant protein from intact cells.
    1 context sentence
    Summary · context
    A method is presented for the direct extraction of the recombinant protein Long-R3-IGF-I from inclusion bodies located in the cytoplasm of intact Escherichia coli cells.

    In this study, IGF-1 LR3 was a lab tool or a carrier, part of a mix, or the subject of a lab method. This result is not evidence about taking IGF-1 LR3.

  2. 02LabNot a test of IGF-1 LR3 aloneOther designOther

    Direct extraction recovered comparable levels of Long-R3-IGF-I as in vitro dissolution after mechanical disruption.

    In plain wordsDirect chemical extraction recovered about as much Long-R3-IGF-I as breaking the bacteria open first.

    Peptide
    IGF-1 LR3
    Outcome
    No significant changeYield of direct extraction versus mechanical disruption
    Study

    Escherichia colin: not reported in source

    intact E. coli cells with cytoplasmic inclusion bodies
    Comparator
    In vitro dissolution after mechanical disruption
    Outcome group
    Synthesis and manufacturing · Pharmacology

    Source sentences

    Summary
    Comparable levels of Long-R3-IGF-I were recovered by direct extraction as achieved by in vitro dissolution following mechanical disruption.

    In this study, IGF-1 LR3 was a lab tool or a carrier, part of a mix, or the subject of a lab method. This result is not evidence about taking IGF-1 LR3.

  3. 03LabNot a test of IGF-1 LR3 aloneOther designOther

    Directly extracted recombinant protein had lower purity than protein from mechanical disruption, because of contamination by bacterial cell components.

    In plain wordsDirectly extracted Long-R3-IGF-I was less pure because of bacterial contamination.

    Peptide
    IGF-1 LR3
    Outcome
    DecreasePurity of directly extracted protein
    Study

    Escherichia colin: not reported in source

    intact E. coli cells with cytoplasmic inclusion bodies
    Comparator
    In vitro dissolution after mechanical disruption
    Outcome group
    Synthesis and manufacturing · Pharmacology

    Source sentences

    Summary
    Comparable levels of Long-R3-IGF-I were recovered by direct extraction as achieved by in vitro dissolution following mechanical disruption. However, the purity of directly extracted recombinant protein was lower due to contamination by bacterial cell components.

    In this study, IGF-1 LR3 was a lab tool or a carrier, part of a mix, or the subject of a lab method. This result is not evidence about taking IGF-1 LR3.

  4. 04LabNot a test of IGF-1 LR3 aloneOther designOther

    Direct extraction of Long-R3-IGF-I followed a first-order equation with a time constant of 3 min.

    In plain wordsDirect extraction of Long-R3-IGF-I followed first-order kinetics with a time constant of 3 minutes.

    Peptide
    IGF-1 LR3
    Outcome
    Not applicableKinetics of direct extraction
    Study

    Escherichia colin: not reported in source

    intact E. coli cells with cytoplasmic inclusion bodies
    Outcome group
    Synthesis and manufacturing · Pharmacology

    Measurements

    time constant
    3 min
    direct extraction

    Source sentences

    Summary
    The kinetics of direct extraction are described using a first-order equation with the time constant of 3 min.

    In this study, IGF-1 LR3 was a lab tool or a carrier, part of a mix, or the subject of a lab method. This result is not evidence about taking IGF-1 LR3.

  5. 05LabNot a test of IGF-1 LR3 aloneOther designOther

    Lower protein release was achieved at lower pH values (below 9) during direct extraction.

    In plain wordsLess Long-R3-IGF-I was released at pH values below 9.

    Peptide
    IGF-1 LR3
    Outcome
    Not applicableEffect of pH on protein release
    Study

    Escherichia colin: not reported in source

    intact E. coli cells with cytoplasmic inclusion bodies
    Outcome group
    Synthesis and manufacturing · Pharmacology

    Source sentences

    Summary
    pH proved to be important with lower levels of protein release achieved at low pH values (<9).

    In this study, IGF-1 LR3 was a lab tool or a carrier, part of a mix, or the subject of a lab method. This result is not evidence about taking IGF-1 LR3.

  6. 06LabNot a test of IGF-1 LR3 aloneOther designOther

    Cell concentration had a minor effect on Long-R3-IGF-I release and caused an observable increase in viscosity.

    In plain wordsCell concentration had a small effect on Long-R3-IGF-I release and made the mixture thicker.

    Peptide
    IGF-1 LR3
    Outcome
    Not applicableEffect of cell concentration on release and viscosity
    Study

    Escherichia colin: not reported in source

    intact E. coli cells with cytoplasmic inclusion bodies
    Outcome group
    Synthesis and manufacturing · Pharmacology

    Source sentences

    Summary
    Cell concentration also had a minor effect on Long-R3-IGF-I release and caused an observable increase in viscosity.

    In this study, IGF-1 LR3 was a lab tool or a carrier, part of a mix, or the subject of a lab method. This result is not evidence about taking IGF-1 LR3.

AI wrote and checked these results. AI can make mistakes, so check important results against the source.

Version history

PepBank keeps every version of the data on this study. Status changes from Retraction Watch, Europe PMC and Crossref are listed with their source.

  1. Version 2Current

    13 Oct 2026, 21:44 UTC

    Study results updated

  2. Version 1

    13 Oct 2026, 16:31 UTC

    First added · source update from Europe PMC

Subjects

Terms as the source lists them.

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