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Study · Not a test of the peptide alone · 1999

3 results

Chemical treatment of Escherichia coli: 3. Selective extraction of a recombinant protein from cytoplasmic inclusion bodies in intact cells.

Falconer RJ, O'Neill BK, Middelberg AP

Biotechnology and bioengineering1 Feb 1999Cited by 18 (Europe PMC)

PMID9921154 (opens in a new tab)DOI10.1002/(sici)1097-0290(19990220)62:4<455::aid-bit8>3.0.co;2-2 (opens in a new tab)PepBankPUB-09921154
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Summary

The text of this summary is not shown here, because the article license does not allow reuse. Read it at the source (opens in a new tab)

Text rights: Publisher copyright. Results still quote short attributed sentences.

Results from this study3

No result from this study comes from a test of the peptide.

Studies that did not test the peptide alone3

In this study, IGF-1 LR3 was a lab tool or a carrier, part of a mix, or the subject of a lab method. These results are not evidence about taking IGF-1 LR3.

  1. 01LabNot a test of IGF-1 LR3 aloneOther designOther

    A two-stage chemical extraction recovered 81% (w/w) of recombinant Long-R3-IGF-I from inclusion bodies in intact E. coli.

    In plain wordsIn lab tests, a two-step chemical process recovered 81% of the made IGF-I analogue from bacteria.

    Peptide
    IGF-1 LR3
    Outcome
    Not applicableRecovery of recombinant Long-R3-IGF-I
    Study

    Escherichia colin: not reported in source

    Intact E. coli cells with cytoplasmic inclusion bodies
    Outcome group
    Synthesis and manufacturing · Pharmacology

    Measurements

    Recovery
    81 % (w/w)
    two-stage extraction

    Source sentences

    Summary
    Extraction using this selective two-stage process yielded an 81% (w/w) recovery of the recombinant protein Long-R3-IGF-I from inclusion bodies located in the cytoplasm of intact E. coli, at a purity of 46% (w/w).
    1 context sentence
    Summary · context
    In the first stage, inclusion-body stability is promoted by the addition of 15 mM 2-hydroxyethyldisulfide (2-HEDS), also known as oxidized beta-mercaptoethanol, to the permeabilization buffer (6 M urea + 3 mM ethylenediaminetetraacetate [EDTA]).

    In this study, IGF-1 LR3 was a lab tool or a carrier, part of a mix, or the subject of a lab method. This result is not evidence about taking IGF-1 LR3.

  2. 02LabNot a test of IGF-1 LR3 aloneOther designOther

    A two-stage chemical extraction gave Long-R3-IGF-I at 46% (w/w) purity.

    In plain wordsIn lab tests, the extracted IGF-I analogue was 46% pure.

    Peptide
    IGF-1 LR3
    Outcome
    Not applicablePurity of extracted Long-R3-IGF-I
    Study

    Escherichia colin: not reported in source

    Intact E. coli cells with cytoplasmic inclusion bodies
    Outcome group
    Synthesis and manufacturing · Pharmacology

    Measurements

    Purity
    46 % (w/w)
    two-stage extraction

    Source sentences

    Summary
    Extraction using this selective two-stage process yielded an 81% (w/w) recovery of the recombinant protein Long-R3-IGF-I from inclusion bodies located in the cytoplasm of intact E. coli, at a purity of 46% (w/w).
    1 context sentence
    Summary · context
    In the first stage, inclusion-body stability is promoted by the addition of 15 mM 2-hydroxyethyldisulfide (2-HEDS), also known as oxidized beta-mercaptoethanol, to the permeabilization buffer (6 M urea + 3 mM ethylenediaminetetraacetate [EDTA]).

    In this study, IGF-1 LR3 was a lab tool or a carrier, part of a mix, or the subject of a lab method. This result is not evidence about taking IGF-1 LR3.

  3. 03LabNot a test of IGF-1 LR3 aloneOther designOther

    Recovery and purity of the two-stage extraction were comparable to conventional extraction.

    In plain wordsIn lab tests, the new extraction process gave results similar to the conventional process.

    Peptide
    IGF-1 LR3
    Outcome
    No significant changeExtraction versus conventional extraction
    Study

    Escherichia colin: not reported in source

    Intact E. coli cells with cytoplasmic inclusion bodies
    Comparator
    Conventional extraction (mechanical disruption, centrifugation, solubilization)
    Outcome group
    Synthesis and manufacturing · Pharmacology

    Source sentences

    Summary
    This was comparable to that achieved by conventional extraction (mechanical disruption followed by centrifugation and solubilization).
    1 context sentence
    Summary · context
    In the first stage, inclusion-body stability is promoted by the addition of 15 mM 2-hydroxyethyldisulfide (2-HEDS), also known as oxidized beta-mercaptoethanol, to the permeabilization buffer (6 M urea + 3 mM ethylenediaminetetraacetate [EDTA]).

    In this study, IGF-1 LR3 was a lab tool or a carrier, part of a mix, or the subject of a lab method. This result is not evidence about taking IGF-1 LR3.

AI wrote and checked these results. AI can make mistakes, so check important results against the source.

Version history

PepBank keeps every version of the data on this study. Status changes from Retraction Watch, Europe PMC and Crossref are listed with their source.

  1. Version 2Current

    13 Oct 2026, 21:44 UTC

    Study results updated

  2. Version 1

    13 Oct 2026, 16:31 UTC

    First added · source update from Europe PMC

Subjects

Terms as the source lists them.

Publication types

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