Direct effects of growth hormone (GH)-releasing hexapeptide (GHRP-6) and GH-releasing factor (GRF) on GH secretion from cultured porcine somatotropes.

Sánchez-Hormigo A, Castaño JP, Torronteras R, Malagón MM, Ramírez JL, Gracia-Navarro F

Life sciences1 Jan 1998Cited by 5 (Europe PMC)

PMID9839531DOI10.1016/s0024-3205(99)80004-6PepBankPUB-09839531

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Summary

The text of this summary is not shown here, because the article license does not allow reuse. Read it at the source (opens in a new tab)

Text rights: Publisher copyright. Results still quote short attributed sentences.

Results from this study6

2 results from studies that gave the peptide. No result from this study comes from people.

01LabIn vitro (cells)Activity measurement
In porcine pituitary monolayers, GHRP‑6 stimulated GH release at concentrations at or above the reported threshold.
In plain wordsIn pig pituitary cells, GHRP‑6 increased growth hormone release.
Peptide
GHRP-6
Outcome
IncreaseGH release from monolayers
Study
pign: not reported in source
Cultured porcine pituitary cells
Outcome group
Growth hormone and IGF-1 · Endocrine and hormones
Studied dose
>=10(-9) MAs given in this study (pig).
Source sentences
Summary
Our results demonstrate that both GHRP-6 and GRF stimulated GH release from monolayer cultures at doses equal to or above 10(-9) M.
Report a problemFND-0983953101
02LabIn vitro (cells)Mechanism
Cell immunoblotting showed that GHRP‑6 acted directly on porcine somatotropes.
In plain wordsIn pig pituitary cells, GHRP‑6 acted directly on growth hormone-producing cells.
Peptide
GHRP-6
Outcome
Not applicableDirect somatotroph action
Study
pign: not reported in source
Cultured porcine pituitary cells
Outcome group
Growth hormone and IGF-1 · Endocrine and hormones
Studied dose
Dose not stated
Source sentences
Summary
Use of cell immunoblot assay demonstrated that, like GRF, the hexapeptide acts directly upon porcine somatotropes to exert its action.
Report a problemFND-0983953102

Studies that did not test the peptide alone4

In this study, GHRP-6 was a lab tool or a carrier, part of a mix, or the subject of a lab method. These results are not evidence about taking GHRP-6.

03LabNot a test of GHRP-6 aloneIn vitro (cells)Activity measurement
In porcine pituitary cultures, GHRP‑6 at 10(-6) M plus GRF produced additive, rather than synergistic, GH release.
In plain wordsIn pig pituitary cells, GHRP‑6 plus GRF produced additive growth hormone release without synergy.
Peptide
GHRP-6
Outcome
IncreaseGH response with GRF at higher GHRP-6 concentration
Study
pign: not reported in source
Cultured porcine pituitary cells
Outcome group
Growth hormone and IGF-1 · Endocrine and hormones
Source sentences
Summary
Moreover, regardless of the technique applied, combined administration of GHRP-6 (10(-6) or 10(-9) M) and GRF (10(-8) M) resulted in an additive, but not synergistic, stimulatory GH response.
In this study, GHRP-6 was a lab tool or a carrier, part of a mix, or the subject of a lab method. This result is not evidence about taking GHRP-6.
Report a problemFND-0983953103
04LabNot a test of GHRP-6 aloneIn vitro (cells)Activity measurement
In porcine pituitary cultures, GHRP‑6 at 10(-9) M plus GRF produced additive, rather than synergistic, GH release.
In plain wordsIn pig pituitary cells, the lower GHRP‑6 concentration plus GRF produced additive growth hormone release without synergy.
Peptide
GHRP-6
Outcome
IncreaseGH response with GRF at lower GHRP-6 concentration
Study
pign: not reported in source
Cultured porcine pituitary cells
Outcome group
Growth hormone and IGF-1 · Endocrine and hormones
Source sentences
Summary
Moreover, regardless of the technique applied, combined administration of GHRP-6 (10(-6) or 10(-9) M) and GRF (10(-8) M) resulted in an additive, but not synergistic, stimulatory GH response.
In this study, GHRP-6 was a lab tool or a carrier, part of a mix, or the subject of a lab method. This result is not evidence about taking GHRP-6.
Report a problemFND-0983953104
05LabNot a test of GHRP-6 aloneIn vitro (cells)Mechanism
In porcine pituitary cells, somatostatin inhibited GH release stimulated by GHRP‑6 alone.
In plain wordsIn pig pituitary cells, somatostatin reduced growth hormone release stimulated by GHRP‑6 alone.
Peptide
GHRP-6
Outcome
DecreaseSomatostatin inhibition of GHRP-6 response
Study
pign: not reported in source
Cultured porcine pituitary cells
Outcome group
Growth hormone and IGF-1 · Endocrine and hormones
Source sentences
Summary
Finally, SRIF (10(-7) M) inhibited the stimulatory effect of GHRP-6 alone or in combination with GRF.
In this study, GHRP-6 was a lab tool or a carrier, part of a mix, or the subject of a lab method. This result is not evidence about taking GHRP-6.
Report a problemFND-0983953105
06LabNot a test of GHRP-6 aloneIn vitro (cells)Mechanism
In porcine pituitary cells, somatostatin inhibited GH release stimulated by GHRP‑6 plus GRF.
In plain wordsIn pig pituitary cells, somatostatin reduced growth hormone release stimulated by GHRP‑6 plus GRF.
Peptide
GHRP-6
Outcome
DecreaseSomatostatin inhibition of combination response
Study
pign: not reported in source
Cultured porcine pituitary cells
Outcome group
Growth hormone and IGF-1 · Endocrine and hormones
Source sentences
Summary
Finally, SRIF (10(-7) M) inhibited the stimulatory effect of GHRP-6 alone or in combination with GRF.
In this study, GHRP-6 was a lab tool or a carrier, part of a mix, or the subject of a lab method. This result is not evidence about taking GHRP-6.
Report a problemFND-0983953106

AI wrote and checked these results. AI can make mistakes, so check important results against the source.

Version history

PepBank keeps every version of the data on this study. Status changes from Retraction Watch, Europe PMC and Crossref are listed with their source.

Version 2Current
21 Oct 2026, 15:58 UTC
Study results updated
Version 1
9 Oct 2026, 03:13 UTC
First added · source update from Europe PMC

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