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Study · Not a test of the peptide alone · 1998

4 results

Development of a sensitive enzymeimmunoassay for oxytocin determination in bovine plasma.

Prakash BS, Metten M, Schams D, Wuttke W

Animal reproduction science1 May 1998Cited by 12 (Europe PMC)

PMID9675400 (opens in a new tab)DOI10.1016/s0378-4320(98)00069-4 (opens in a new tab)PepBankPUB-09675400
Publisher pagePubMedAsk about this study
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Summary

The text of this summary is not shown here, because the article license does not allow reuse. Read it at the source (opens in a new tab)

Text rights: Publisher copyright. Results still quote short attributed sentences.

Results from this study4

No result from this study comes from a test of the peptide.

Studies that did not test the peptide alone4

In this study, Oxytocin was a lab tool or a carrier, part of a mix, or the subject of a lab method. These results are not evidence about taking Oxytocin.

  1. 01LabNot a test of Oxytocin aloneOther designOther

    In plasma samples from four cows, oxytocin concentrations measured by the new enzymeimmunoassay and by radioimmunoassay were highly correlated (r = 0.97).

    In plain wordsIn cow plasma, the new oxytocin test gave results very close to those of an older radioactive test.

    Peptide
    Oxytocin
    Outcome
    Not applicableAgreement between enzymeimmunoassay and radioimmunoassay
    Study

    cattlen = 4

    bovine plasma
    Outcome group
    Analytical methods and detection · Pharmacology

    Measurements

    Correlation EIA vs RIA
    0.97 r
    four cows

    Source sentences

    Summary
    The oxytocin concentrations assayed by EIA and RIA in plasma samples obtained from four cows before, during and after milking were highly correlated and very similar (r = 0.97).
    2 context sentences
    Summary · context
    A highly sensitive and specific second antibody enzymeimmunoassay (EIA) on microtiterplates for oxytocin determination in bovine plasma using the biotin-streptavidin amplification system was developed.
    Summary · context
    The assay was compared with a radioimmunoassay (RIA) procedure employing prior solvent extraction of plasma samples.

    In this study, Oxytocin was a lab tool or a carrier, part of a mix, or the subject of a lab method. This result is not evidence about taking Oxytocin.

  2. 02LabNot a test of Oxytocin aloneOther designOther

    In bovine plasma, the new oxytocin enzymeimmunoassay had a sensitivity of 0.25 pg/well, equal to 1.25 pg/ml plasma.

    In plain wordsIn cow plasma, the new test could detect oxytocin down to 1.25 pg/ml.

    Peptide
    Oxytocin
    Outcome
    Not applicableEnzymeimmunoassay sensitivity for oxytocin
    Study

    cattlen: not reported in source

    bovine plasma
    Outcome group
    Analytical methods and detection · Pharmacology

    Measurements

    Assay sensitivity
    0.25 pg/well
    oxytocin EIA
    Assay sensitivity
    1.25 pg/ml
    plasma

    Source sentences

    Summary
    The sensitivity of the assay was 0.25 pg/well which corresponded to 1.25 pg/ml plasma
    2 context sentences
    Summary · context
    A highly sensitive and specific second antibody enzymeimmunoassay (EIA) on microtiterplates for oxytocin determination in bovine plasma using the biotin-streptavidin amplification system was developed.
    Summary · context
    The assay was compared with a radioimmunoassay (RIA) procedure employing prior solvent extraction of plasma samples.

    In this study, Oxytocin was a lab tool or a carrier, part of a mix, or the subject of a lab method. This result is not evidence about taking Oxytocin.

  3. 03LabNot a test of Oxytocin aloneOther designOther

    The oxytocin antiserum in the enzymeimmunoassay showed no significant cross-reaction with other octapeptides tested.

    In plain wordsIn lab tests, the oxytocin test did not react with other similar small peptides that were tested.

    Peptide
    Oxytocin
    Outcome
    No significant changeCross-reactivity of oxytocin antiserum
    Study

    cattlen: not reported in source

    bovine plasma
    Outcome group
    Analytical methods and detection · Pharmacology

    Source sentences

    Summary
    The oxytocin antiserum used in the assay showed no significant cross-reaction with other octapeptides tested.
    2 context sentences
    Summary · context
    A highly sensitive and specific second antibody enzymeimmunoassay (EIA) on microtiterplates for oxytocin determination in bovine plasma using the biotin-streptavidin amplification system was developed.
    Summary · context
    The assay was compared with a radioimmunoassay (RIA) procedure employing prior solvent extraction of plasma samples.

    In this study, Oxytocin was a lab tool or a carrier, part of a mix, or the subject of a lab method. This result is not evidence about taking Oxytocin.

  4. 04LabNot a test of Oxytocin aloneOther designOther

    Plasma volumes of 50 to 200 microliters did not influence the shape of the oxytocin standard curve, although higher volumes lowered the OD450.

    In plain wordsIn lab tests, plasma volumes from 50 to 200 microliters did not change the shape of the oxytocin test curve.

    Peptide
    Oxytocin
    Outcome
    No significant changeEffect of plasma volume on the standard curve
    Study

    cattlen: not reported in source

    bovine plasma
    Outcome group
    Analytical methods and detection · Pharmacology

    Measurements

    Plasma volume range
    50 to 200 microliters
    EIA

    Source sentences

    Summary
    Plasma volumes for the assay ranging from 50 to 200 microliters did not influence the shape of the oxytocin standard curve; however a distinct drop in the OD450 was observed with higher plasma volumes.
    2 context sentences
    Summary · context
    A highly sensitive and specific second antibody enzymeimmunoassay (EIA) on microtiterplates for oxytocin determination in bovine plasma using the biotin-streptavidin amplification system was developed.
    Summary · context
    The assay was compared with a radioimmunoassay (RIA) procedure employing prior solvent extraction of plasma samples.

    In this study, Oxytocin was a lab tool or a carrier, part of a mix, or the subject of a lab method. This result is not evidence about taking Oxytocin.

AI wrote and checked these results. AI can make mistakes, so check important results against the source.

Version history

PepBank keeps every version of the data on this study. Status changes from Retraction Watch, Europe PMC and Crossref are listed with their source.

  1. Version 2Current

    11 Oct 2026, 20:03 UTC

    Study results updated

  2. Version 1

    9 Oct 2026, 02:51 UTC

    First added · source update from Europe PMC

Subjects

Terms as the source lists them.

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