Mechanisms of action of growth hormone-releasing peptide-2 in bovine pituitary cells.

Roh SG, He ML, Matsunaga N, Hidaka S, Hidari H

Journal of animal science1 Oct 1997Cited by 9 (Europe PMC)

PMID9331879DOI10.2527/1997.75102744xPepBankPUB-09331879

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Summary

The text of this summary is not shown here, because the article license does not allow reuse. Read it at the source (opens in a new tab)

Text rights: Publisher copyright. Results still quote short attributed sentences.

Results from this study7

1 result from studies that gave the peptide. No result from this study comes from people.

01LabIn vitro (cells)Activity measurement
In bovine anterior pituitary cells, GHRP‑2 at doses from 10(-13) to 10(-7) M increased GH secretion.
In plain wordsIn cow pituitary cells in culture, GHRP‑2 increased growth hormone release across a wide range of doses.
Peptide
GHRP-2
Outcome
IncreaseGH secretion
Study
cattlen: not reported in source
Bovine anterior pituitary primary cell culture
Outcome group
Growth hormone and IGF-1 · Endocrine and hormones
Studied dose
10(-13) to 10(-7) MAs given in this study (cattle).
Source sentences
Summary
Doses of GHRP-2 from 10(-13) to 10(-7) M) increased (P < .05) GH secretion.
1 context sentence(design, population, supporting numbers)
Summary · context
We conducted this study to investigate the mechanisms of action of growth hormone-releasing peptide-2 (D-Ala-D-beta Nal-Ala-Trp-D-Phe-Lys-NH2; GHRP-2) in bovine anterior pituitary primary cell culture.
Report a problemFND-0933187901

Studies that did not test the peptide alone6

In this study, GHRP-2 was a lab tool or a carrier, part of a mix, or the subject of a lab method. These results are not evidence about taking GHRP-2.

02LabNot a test of GHRP-2 aloneIn vitro (cells)Activity measurement
In bovine anterior pituitary cells, GHRP‑2 and GRF given together had an additive effect on GH release.
In plain wordsIn cow pituitary cells in culture, GHRP‑2 and GRF together had an additive effect on growth hormone release.
Peptide
GHRP-2
Outcome
IncreaseGH release with GHRP-2 plus GRF
Study
cattlen: not reported in source
Bovine anterior pituitary primary cell culture
Outcome group
Growth hormone and IGF-1 · Endocrine and hormones
Source sentences
Summary
The GHRP-2 (10(-7) M) and GH-releasing factor (GRF; 10(-7) M) administered together had an additive effect on the release of GH (P < .05).
1 context sentence(design, population, supporting numbers)
Summary · context
We conducted this study to investigate the mechanisms of action of growth hormone-releasing peptide-2 (D-Ala-D-beta Nal-Ala-Trp-D-Phe-Lys-NH2; GHRP-2) in bovine anterior pituitary primary cell culture.
In this study, GHRP-2 was a lab tool or a carrier, part of a mix, or the subject of a lab method. This result is not evidence about taking GHRP-2.
Report a problemFND-0933187902
03LabNot a test of GHRP-2 aloneIn vitro (cells)Mechanism
In bovine anterior pituitary cells, somatostatin decreased the GH secretion in response to GHRP‑2.
In plain wordsIn cow pituitary cells in culture, somatostatin lowered the growth hormone release caused by GHRP‑2.
Peptide
GHRP-2
Outcome
DecreaseGH secretion after somatostatin
Study
cattlen: not reported in source
Bovine anterior pituitary primary cell culture
Outcome group
Growth hormone and IGF-1 · Endocrine and hormones
Source sentences
Summary
Somatostatin (1 microM) decreased GH secretion in response to GHRP-2 and(or) GRF (P < .05).
1 context sentence(design, population, supporting numbers)
Summary · context
We conducted this study to investigate the mechanisms of action of growth hormone-releasing peptide-2 (D-Ala-D-beta Nal-Ala-Trp-D-Phe-Lys-NH2; GHRP-2) in bovine anterior pituitary primary cell culture.
In this study, GHRP-2 was a lab tool or a carrier, part of a mix, or the subject of a lab method. This result is not evidence about taking GHRP-2.
Report a problemFND-0933187903
04LabNot a test of GHRP-2 aloneIn vitro (cells)Mechanism
In bovine anterior pituitary cells, a GRF receptor antagonist blocked the GH secretion in response to GHRP‑2.
In plain wordsIn cow pituitary cells in culture, blocking the GRF receptor stopped growth hormone release caused by GHRP‑2.
Peptide
GHRP-2
Outcome
DecreaseGH secretion after GRF receptor antagonist
Study
cattlen: not reported in source
Bovine anterior pituitary primary cell culture
Outcome group
Growth hormone and IGF-1 · Endocrine and hormones
Source sentences
Summary
Secretion of GH in response to GHRP-2 was blocked (P < .01) by a GRF receptor antagonist (.1 microM).
1 context sentence(design, population, supporting numbers)
Summary · context
We conducted this study to investigate the mechanisms of action of growth hormone-releasing peptide-2 (D-Ala-D-beta Nal-Ala-Trp-D-Phe-Lys-NH2; GHRP-2) in bovine anterior pituitary primary cell culture.
In this study, GHRP-2 was a lab tool or a carrier, part of a mix, or the subject of a lab method. This result is not evidence about taking GHRP-2.
Report a problemFND-0933187904
05LabNot a test of GHRP-2 aloneIn vitro (cells)Mechanism
In bovine anterior pituitary cells, the Ca2+ channel blocker nifedipine inhibited GHRP‑2-stimulated GH release.
In plain wordsIn cow pituitary cells in culture, a calcium channel blocker reduced the growth hormone release caused by GHRP‑2.
Peptide
GHRP-2
Outcome
DecreaseGH release after nifedipine
Study
cattlen: not reported in source
Bovine anterior pituitary primary cell culture
Outcome group
Growth hormone and IGF-1 · Endocrine and hormones
Source sentences
Summary
Nifedipine (10 microM), a voltage-dependent Ca2+ channel blocker, inhibited (P < .01) GHRP-2-stimulated GH release.
1 context sentence(design, population, supporting numbers)
Summary · context
We conducted this study to investigate the mechanisms of action of growth hormone-releasing peptide-2 (D-Ala-D-beta Nal-Ala-Trp-D-Phe-Lys-NH2; GHRP-2) in bovine anterior pituitary primary cell culture.
In this study, GHRP-2 was a lab tool or a carrier, part of a mix, or the subject of a lab method. This result is not evidence about taking GHRP-2.
Report a problemFND-0933187905
06LabNot a test of GHRP-2 aloneIn vitro (cells)Mechanism
In bovine anterior pituitary cells, the GH release in response to GHRP‑2 and the protein kinase C activator PMA was additive.
In plain wordsIn cow pituitary cells in culture, GHRP‑2 and a protein kinase C activator had an additive effect on growth hormone release.
Peptide
GHRP-2
Outcome
IncreaseGH release with GHRP-2 and PMA
Study
cattlen: not reported in source
Bovine anterior pituitary primary cell culture
Outcome group
Growth hormone and IGF-1 · Endocrine and hormones
Source sentences
Summary
The GH release in response to GHRP-2 and 4 beta-phorbol-12-myristate-13-acetate (10(-7) M), a protein kinase C activator, was additive (P < .01).
1 context sentence(design, population, supporting numbers)
Summary · context
We conducted this study to investigate the mechanisms of action of growth hormone-releasing peptide-2 (D-Ala-D-beta Nal-Ala-Trp-D-Phe-Lys-NH2; GHRP-2) in bovine anterior pituitary primary cell culture.
In this study, GHRP-2 was a lab tool or a carrier, part of a mix, or the subject of a lab method. This result is not evidence about taking GHRP-2.
Report a problemFND-0933187906
07LabNot a test of GHRP-2 aloneIn vitro (cells)Mechanism
In bovine anterior pituitary cells, forskolin further stimulated the GH release in response to GHRP‑2.
In plain wordsIn cow pituitary cells in culture, forskolin, which raises cAMP, increased the growth hormone release caused by GHRP‑2 even more.
Peptide
GHRP-2
Outcome
IncreaseGH release with GHRP-2 and forskolin
Study
cattlen: not reported in source
Bovine anterior pituitary primary cell culture
Outcome group
Growth hormone and IGF-1 · Endocrine and hormones
Source sentences
Summary
Forskolin (30 microM), a cAMP elevating agent, further stimulated (P < .01) the GH release in response to GHRP-2.
1 context sentence(design, population, supporting numbers)
Summary · context
We conducted this study to investigate the mechanisms of action of growth hormone-releasing peptide-2 (D-Ala-D-beta Nal-Ala-Trp-D-Phe-Lys-NH2; GHRP-2) in bovine anterior pituitary primary cell culture.
In this study, GHRP-2 was a lab tool or a carrier, part of a mix, or the subject of a lab method. This result is not evidence about taking GHRP-2.
Report a problemFND-0933187907

AI wrote and checked these results. AI can make mistakes, so check important results against the source.

Version history

PepBank keeps every version of the data on this study. Status changes from Retraction Watch, Europe PMC and Crossref are listed with their source.

Version 2Current
13 Oct 2026, 21:44 UTC
Study results updated
Version 1
13 Oct 2026, 16:31 UTC
First added · source update from Europe PMC

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