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Study · In vitro (cells) · sheep · 1997

10 results

The role of protein kinase C in GH secretion induced by GH-releasing factor and GH-releasing peptides in cultured ovine somatotrophs.

Wu D, Clarke IJ, Chen C

The Journal of endocrinology1 Aug 1997Cited by 27 (Europe PMC)

PMID9291832 (opens in a new tab)DOI10.1677/joe.0.1540219 (opens in a new tab)PepBankPUB-09291832
Publisher pagePubMedAsk about this study
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Summary

The text of this summary is not shown here, because the article license does not allow reuse. Read it at the source (opens in a new tab)

Text rights: Publisher copyright. Results still quote short attributed sentences.

Results from this study10

3 results from studies that gave the peptide. No result from this study comes from people.

  1. 01LabIn vitro (cells)Mechanism

    In cultured ovine somatotrophs, GHRP‑2 caused translocation of protein kinase C activity from the cytosol to the cell membranes.

    In plain wordsIn sheep pituitary cells in culture, GHRP‑2 moved the enzyme protein kinase C from the cell fluid to the cell membrane.

    Peptide
    GHRP-2
    Outcome
    IncreasePKC translocation from cytosol to membrane
    Study

    sheepn: not reported in source

    Primary culture of ovine somatotrophs
    Outcome group
    Receptor activity and mechanism · Endocrine and hormones
    Studied dose
    Dose not stated

    Source sentences

    Summary
    In partially purified sheep somatotrophs, GRF and GHRP-2 caused translocation of PKC activity from the cytosol to the cell membranes and caused GH release in a dose- and time-dependent manner.
    1 context sentence
    Summary · context
    The involvement of protein kinase C (PKC) in the action of GH-releasing factor (GRF) and synthetic GH-releasing peptides (GHRP-2 and GHRP-6) was investigated in ovine somatotrophs in primary culture.
  2. 02LabIn vitro (cells)Activity measurement

    In cultured ovine somatotrophs, GHRP‑2 caused GH release in a dose- and time-dependent manner.

    In plain wordsIn sheep pituitary cells in culture, GHRP‑2 made the cells release growth hormone, and more so with higher dose and longer time.

    Peptide
    GHRP-2
    Outcome
    IncreaseGH release
    Study

    sheepn: not reported in source

    Primary culture of ovine somatotrophs
    Outcome group
    Growth hormone and IGF-1 · Endocrine and hormones
    Studied dose
    Dose not stated

    Source sentences

    Summary
    In partially purified sheep somatotrophs, GRF and GHRP-2 caused translocation of PKC activity from the cytosol to the cell membranes and caused GH release in a dose- and time-dependent manner.
    1 context sentence
    Summary · context
    The involvement of protein kinase C (PKC) in the action of GH-releasing factor (GRF) and synthetic GH-releasing peptides (GHRP-2 and GHRP-6) was investigated in ovine somatotrophs in primary culture.
  3. 03LabIn vitro (cells)Mechanism

    In cultured ovine somatotrophs, GHRP‑6 did not cause PKC translocation.

    In plain wordsIn sheep pituitary cells in culture, GHRP‑6 did not move protein kinase C to the cell membrane.

    Peptide
    GHRP-6
    Outcome
    No significant changePKC translocation from cytosol to membrane
    Study

    sheepn: not reported in source

    Primary culture of ovine somatotrophs
    Outcome group
    Receptor activity and mechanism · Endocrine and hormones
    Studied dose
    Dose not stated

    Source sentences

    Summary
    GHRP-6 did not cause PKC translocation.
    1 context sentence
    Summary · context
    The involvement of protein kinase C (PKC) in the action of GH-releasing factor (GRF) and synthetic GH-releasing peptides (GHRP-2 and GHRP-6) was investigated in ovine somatotrophs in primary culture.

Studies that did not test the peptide alone7

In this study, the peptide was a lab tool or a carrier, part of a mix, or the subject of a lab method. These results are not evidence about taking the peptide.

  1. 04LabNot a test of GHRP-2 aloneIn vitro (cells)Activity measurement

    In cultured ovine somatotrophs, PKC inhibitors partially reduced the GH release caused by GHRP‑2.

    In plain wordsIn sheep pituitary cells in culture, blocking protein kinase C partly reduced the growth hormone release caused by GHRP‑2.

    Peptide
    GHRP-2
    Outcome
    DecreaseGH release after PKC inhibitors
    Study

    sheepn: not reported in source

    Primary culture of ovine somatotrophs
    Outcome group
    Growth hormone and IGF-1 · Endocrine and hormones

    Source sentences

    Summary
    The PKC inhibitors, calphostin C, staurosporine and chelerythrine, partially reduced GH release in response to GRF and GHRP-2 at doses which selectively inhibit PKC activity.
    1 context sentence
    Summary · context
    The involvement of protein kinase C (PKC) in the action of GH-releasing factor (GRF) and synthetic GH-releasing peptides (GHRP-2 and GHRP-6) was investigated in ovine somatotrophs in primary culture.

    In this study, GHRP-2 was a lab tool or a carrier, part of a mix, or the subject of a lab method. This result is not evidence about taking GHRP-2.

  2. 05LabNot a test of GHRP-2 aloneIn vitro (cells)Mechanism

    In cultured ovine somatotrophs, down-regulation of PKC totally abolished PKC translocation in response to a GHRP‑2 challenge.

    In plain wordsIn sheep pituitary cells in culture, removing protein kinase C activity stopped GHRP‑2 from moving the enzyme to the membrane.

    Peptide
    GHRP-2
    Outcome
    DecreasePKC translocation after PKC down-regulation
    Study

    sheepn: not reported in source

    Primary culture of ovine somatotrophs
    Outcome group
    Receptor activity and mechanism · Endocrine and hormones

    Source sentences

    Summary
    Down-regulation of PKC by the treatment of cells with phorbol 12,13-dibutyrate for 16 h caused a significant (P < 0.001) reduction in total PKC activity and totally abolished PKC translocation in response to a challenge with GRF, GHRP-2 or PMA.
    1 context sentence
    Summary · context
    The involvement of protein kinase C (PKC) in the action of GH-releasing factor (GRF) and synthetic GH-releasing peptides (GHRP-2 and GHRP-6) was investigated in ovine somatotrophs in primary culture.

    In this study, GHRP-2 was a lab tool or a carrier, part of a mix, or the subject of a lab method. This result is not evidence about taking GHRP-2.

  3. 06LabNot a test of GHRP-2 aloneIn vitro (cells)Activity measurement

    In cultured ovine somatotrophs, down-regulation of PKC abolished GH release in response to GHRP‑2.

    In plain wordsIn sheep pituitary cells in culture, removing protein kinase C activity stopped GHRP‑2 from causing growth hormone release.

    Peptide
    GHRP-2
    Outcome
    DecreaseGH release after PKC down-regulation
    Study

    sheepn: not reported in source

    Primary culture of ovine somatotrophs
    Outcome group
    Growth hormone and IGF-1 · Endocrine and hormones

    Source sentences

    Summary
    In addition, down-regulation abolished GH release in response to GRF, GHRP-2 or GHRP-6.
    1 context sentence
    Summary · context
    The involvement of protein kinase C (PKC) in the action of GH-releasing factor (GRF) and synthetic GH-releasing peptides (GHRP-2 and GHRP-6) was investigated in ovine somatotrophs in primary culture.

    In this study, GHRP-2 was a lab tool or a carrier, part of a mix, or the subject of a lab method. This result is not evidence about taking GHRP-2.

  4. 07LabNot a test of GHRP-6 aloneIn vitro (cells)Activity measurement

    In cultured ovine somatotrophs, down-regulation of PKC abolished GH release in response to GHRP‑6.

    In plain wordsIn sheep pituitary cells in culture, removing protein kinase C activity stopped GHRP‑6 from causing growth hormone release.

    Peptide
    GHRP-6
    Outcome
    DecreaseGH release after PKC down-regulation
    Study

    sheepn: not reported in source

    Primary culture of ovine somatotrophs
    Outcome group
    Growth hormone and IGF-1 · Endocrine and hormones

    Source sentences

    Summary
    In addition, down-regulation abolished GH release in response to GRF, GHRP-2 or GHRP-6.
    1 context sentence
    Summary · context
    The involvement of protein kinase C (PKC) in the action of GH-releasing factor (GRF) and synthetic GH-releasing peptides (GHRP-2 and GHRP-6) was investigated in ovine somatotrophs in primary culture.

    In this study, GHRP-6 was a lab tool or a carrier, part of a mix, or the subject of a lab method. This result is not evidence about taking GHRP-6.

  5. 08LabNot a test of GHRP-2 aloneIn vitro (cells)Activity measurement

    In cultured ovine somatotrophs, the PKA inhibitor H89 totally blocked the GH release caused by GHRP‑2.

    In plain wordsIn sheep pituitary cells in culture, blocking protein kinase A with H89 completely stopped growth hormone release caused by GHRP‑2.

    Peptide
    GHRP-2
    Outcome
    DecreaseGH release after PKA inhibitor H89
    Study

    sheepn: not reported in source

    Primary culture of ovine somatotrophs
    Outcome group
    Growth hormone and IGF-1 · Endocrine and hormones

    Source sentences

    Summary
    Treatment of cells with H89, a selective PKA inhibitor, totally blocked GH release caused by either GRF or GHRP-2 and partially reduced PMA-induced GH release.
    1 context sentence
    Summary · context
    The involvement of protein kinase C (PKC) in the action of GH-releasing factor (GRF) and synthetic GH-releasing peptides (GHRP-2 and GHRP-6) was investigated in ovine somatotrophs in primary culture.

    In this study, GHRP-2 was a lab tool or a carrier, part of a mix, or the subject of a lab method. This result is not evidence about taking GHRP-2.

  6. 09LabNot a test of GHRP-2 aloneIn vitro (cells)Mechanism

    In cultured ovine somatotrophs, H89 had no effect on PKC translocation caused by GHRP‑2.

    In plain wordsIn sheep pituitary cells in culture, H89 did not change how GHRP‑2 moved protein kinase C to the cell membrane.

    Peptide
    GHRP-2
    Outcome
    No significant changePKC translocation after PKA inhibitor H89
    Study

    sheepn: not reported in source

    Primary culture of ovine somatotrophs
    Outcome group
    Receptor activity and mechanism · Endocrine and hormones

    Source sentences

    Summary
    H89 had no effect on PKC translocation caused by GRF, GHRP-2 or PMA and did not affect GH release caused by GHRP-6.
    1 context sentence
    Summary · context
    The involvement of protein kinase C (PKC) in the action of GH-releasing factor (GRF) and synthetic GH-releasing peptides (GHRP-2 and GHRP-6) was investigated in ovine somatotrophs in primary culture.

    In this study, GHRP-2 was a lab tool or a carrier, part of a mix, or the subject of a lab method. This result is not evidence about taking GHRP-2.

  7. 10LabNot a test of GHRP-6 aloneIn vitro (cells)Activity measurement

    In cultured ovine somatotrophs, H89 did not affect the GH release caused by GHRP‑6.

    In plain wordsIn sheep pituitary cells in culture, H89 did not change growth hormone release caused by GHRP‑6.

    Peptide
    GHRP-6
    Outcome
    No significant changeGH release after PKA inhibitor H89
    Study

    sheepn: not reported in source

    Primary culture of ovine somatotrophs
    Outcome group
    Growth hormone and IGF-1 · Endocrine and hormones

    Source sentences

    Summary
    and did not affect GH release caused by GHRP-6.
    1 context sentence
    Summary · context
    The involvement of protein kinase C (PKC) in the action of GH-releasing factor (GRF) and synthetic GH-releasing peptides (GHRP-2 and GHRP-6) was investigated in ovine somatotrophs in primary culture.

    In this study, GHRP-6 was a lab tool or a carrier, part of a mix, or the subject of a lab method. This result is not evidence about taking GHRP-6.

AI wrote and checked these results. AI can make mistakes, so check important results against the source.

Version history

PepBank keeps every version of the data on this study. Status changes from Retraction Watch, Europe PMC and Crossref are listed with their source.

  1. Version 3Current

    13 Oct 2026, 21:44 UTC

    Study results updated

  2. Version 2

    13 Oct 2026, 16:31 UTC

    Study data updated · source update from Europe PMC

  3. Version 1

    9 Oct 2026, 03:13 UTC

    First added · source update from Europe PMC

Subjects

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