In SKHEP-1 cells, Des(1‑3)-IGF-I increased conditioned-medium IGFBP-3 concentrations dose dependently by more than threefold.
In plain wordsIn cells, Des(1‑3)-IGF-I increased IGFBP-3 protein in the culture medium.
- Peptide
- IGF-1 DES (des(1-3)IGF-I)
- Outcome
- IncreaseIGFBP-3 protein concentration
- Study
humann: not reported in source
SKHEP-1 human hepatocarcinoma cells- Outcome group
- Growth hormone and IGF-1 · Endocrine and hormones
- Studied dose
- 1-100 ng/mlAs given in this study (human).
Measurements
- IGFBP-3 increase
- > 3 fold
- Des(1-3)-IGF-I treatment
Source sentences
SKHEP-1 cells significantly increased the IGFBP-3 concentrations in conditioned medium (CM) when treated with GH (0.1-10 ng/ml), IGF-I (1-100 ng/ml), or Des(1-3)-IGF-I (1-100 ng/ml) in a dose-dependent manner (>3-fold).
1 context sentence(design, population, supporting numbers)
This study investigates the regulation of IGFBP-3 protein, measured by RIA and Western ligand blot, and messenger RNA (mRNA) expression, measured by Northern analysis and reverse transcriptase-PCR, in SKHEP-1 human hepatocarcinoma cells.