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Study · Not a test of the peptide alone · 1996

4 results

Interaction of oxytocin with Ca2+: I. CD and fluorescence spectral characterization and comparison with vasopressin.

Ananthanarayanan VS, Brimble KS

Biopolymers1 Jan 1996Cited by 7 (Europe PMC)

PMID9062067 (opens in a new tab)DOI10.1002/(sici)1097-0282(1996)40:5%3c433::aid-bip2%3e3.0.co;2-x (opens in a new tab)PepBankPUB-09062067
Publisher pagePubMedAsk about this study
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Summary

The text of this summary is not shown here, because the article license does not allow reuse. Read it at the source (opens in a new tab)

Text rights: Publisher copyright. Results still quote short attributed sentences.

Results from this study4

No result from this study comes from a test of the peptide.

Studies that did not test the peptide alone4

In this study, Oxytocin was a lab tool or a carrier, part of a mix, or the subject of a lab method. These results are not evidence about taking Oxytocin.

  1. 01LabNot a test of Oxytocin aloneIn vitro (cell-free)Activity measurement

    In trifluoroethanol, oxytocin bound Ca2+ (Kd approximately 50 microM), causing CD and fluorescence changes that led to a helical conformation.

    In plain wordsIn lab tests, calcium bound to oxytocin and shifted it toward a helical shape.

    Peptide
    Oxytocin
    Outcome
    Not applicableCa2+ binding and conformation of oxytocin
    Study

    n: not reported in source

    trifluoroethanol, CD and fluorescence spectroscopy
    Outcome group
    Receptor activity and mechanism · Pharmacology

    Measurements

    Kd
    ≈ 50 µM
    Ca2+ binding to oxytocin in trifluoroethanol

    Source sentences

    Summary
    Binding Ca2+ (Kd approximately 50 microM) caused drastic CD and fluorescence changes leading to a helical conformation.
    1 context sentence
    Summary · context
    To characterize the cation-bound form of oxytocin, we monitored the binding of Ca2+ and Mg2+ to oxytocin as well as peptides representing its ring and tail regions in trifluoroethanol, a lipid-mimetic solvent, using CD and fluorescence spectroscopy.

    In this study, Oxytocin was a lab tool or a carrier, part of a mix, or the subject of a lab method. This result is not evidence about taking Oxytocin.

  2. 02LabNot a test of Oxytocin aloneIn vitro (cell-free)Activity measurement

    In trifluoroethanol, Mg2+ caused CD changes in oxytocin that were smaller than, and opposite to, those caused by Ca2+.

    In plain wordsIn lab tests, magnesium changed the oxytocin spectrum less than calcium did, and in the opposite direction.

    Peptide
    Oxytocin
    Outcome
    Not applicableMg2+ binding effect on oxytocin CD
    Study

    n: not reported in source

    trifluoroethanol, CD and fluorescence spectroscopy
    Outcome group
    Receptor activity and mechanism · Pharmacology

    Source sentences

    Summary
    Mg2+ caused CD changes smaller than and opposite to Ca2+.
    1 context sentence
    Summary · context
    To characterize the cation-bound form of oxytocin, we monitored the binding of Ca2+ and Mg2+ to oxytocin as well as peptides representing its ring and tail regions in trifluoroethanol, a lipid-mimetic solvent, using CD and fluorescence spectroscopy.

    In this study, Oxytocin was a lab tool or a carrier, part of a mix, or the subject of a lab method. This result is not evidence about taking Oxytocin.

  3. 03LabNot a test of Oxytocin aloneIn vitro (cell-free)Activity measurement

    In trifluoroethanol, the helical structure of oxytocin was enhanced when Ca2+ and Mg2+ were present together.

    In plain wordsIn lab tests, oxytocin took a more helical shape when calcium and magnesium were both present.

    Peptide
    Oxytocin
    Outcome
    IncreaseCombined Ca2+ and Mg2+ effect on oxytocin helicity
    Study

    n: not reported in source

    trifluoroethanol, CD and fluorescence spectroscopy
    Outcome group
    Receptor activity and mechanism · Pharmacology

    Source sentences

    Summary
    However, the helical structure was enhanced when both Ca2+ and Mg2+ were present together.
    1 context sentence
    Summary · context
    To characterize the cation-bound form of oxytocin, we monitored the binding of Ca2+ and Mg2+ to oxytocin as well as peptides representing its ring and tail regions in trifluoroethanol, a lipid-mimetic solvent, using CD and fluorescence spectroscopy.

    In this study, Oxytocin was a lab tool or a carrier, part of a mix, or the subject of a lab method. This result is not evidence about taking Oxytocin.

  4. 04LabNot a test of Oxytocin aloneIn vitro (cell-free)Activity measurement

    In trifluoroethanol, the cation-bound CD spectra of vasopressin differed markedly from those of oxytocin, although the cation-free spectra were similar.

    In plain wordsIn lab tests, vasopressin responded to calcium and magnesium differently from oxytocin, compared with oxytocin.

    Peptide
    Oxytocin
    Outcome
    Not applicableCation-bound CD spectra of oxytocin versus vasopressin
    Study

    n: not reported in source

    trifluoroethanol, CD and fluorescence spectroscopy
    Comparator
    Vasopressin
    Outcome group
    Receptor activity and mechanism · Pharmacology

    Source sentences

    Summary
    While vasopressin displayed a CD spectrum similar to that of oxytocin, CD spectra of its cation-bound forms were markedly different from those of oxytocin; the Ca(2+)-induced CD changes in vasopressin were very much smaller and of opposite sign, and Mg(2+)-induced ones significantly larger than in oxytocin.
    1 context sentence
    Summary · context
    To characterize the cation-bound form of oxytocin, we monitored the binding of Ca2+ and Mg2+ to oxytocin as well as peptides representing its ring and tail regions in trifluoroethanol, a lipid-mimetic solvent, using CD and fluorescence spectroscopy.

    In this study, Oxytocin was a lab tool or a carrier, part of a mix, or the subject of a lab method. This result is not evidence about taking Oxytocin.

AI wrote and checked these results. AI can make mistakes, so check important results against the source.

Version history

PepBank keeps every version of the data on this study. Status changes from Retraction Watch, Europe PMC and Crossref are listed with their source.

  1. Version 2Current

    11 Oct 2026, 20:03 UTC

    Study results updated

  2. Version 1

    9 Oct 2026, 02:51 UTC

    First added · source update from Europe PMC

Subjects

Terms as the source lists them.

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