Effect of intravenous or intracerebroventricular injections of His-D-Trp-Ala-Trp-D-Phe-Lys-NH2 on GH release in conscious, freely moving male rats.

Yagi H, Kaji H, Sato M, Okimura Y, Chihara K

Neuroendocrinology1 Feb 1996Cited by 11 (Europe PMC)

PMID9053785DOI10.1159/000126958PepBankPUB-09053785

Publisher page PubMedAsk about this study

Summary

The text of this summary is not shown here, because the article license does not allow reuse. Read it at the source (opens in a new tab)

Text rights: Publisher copyright. Results still quote short attributed sentences.

Results from this study14

5 results from studies that gave the peptide. No result from this study comes from people.

01AnimalAnimal study (in vivo)Mechanism
In rats, intracerebroventricular GHRP suppressed natural plasma GH fluctuations in a dose-related manner.
In plain wordsIn rats, GHRP injected into a brain ventricle suppressed natural growth hormone fluctuations, with a dose-related effect.
Peptide
GHRP-6
Outcome
DecreaseNatural GH fluctuations
Study
ratn: not reported in source
conscious freely moving male rats
Outcome group
Growth hormone and IGF-1 · Endocrine and hormones
Studied dose
1, 3 and 10 mu g/rat · intracerebroventricular · more than 1 hAs given in this study (rat).
Source sentences
Summary
A significant and dose-related suppression of natural fluctuations in plasma GH occurred immediately after intracerebroventricular injection of GHRP at the doses of 1, 3 and 10 mu g/rat and was sustained for more than 1 h.
1 context sentence(design, population, supporting numbers)
Summary · context
The present study was designed to examine the effects of intracerebroventricular injection of His-D-Trp-Ala-Trp-D-Phe-Lys-NH2 (GHRP) on GH secretion in freely moving conscious male rats and to determine whether the central action of GHRP is mediated by increased GHRH and/or somatostatin (SRIF) release.
Report a problemFND-0905378501
02AnimalAnimal study (in vivo)Mechanism
In rats, intracerebroventricular GHRP suppressed plasma GH rises after intravenous GHRH analog.
In plain wordsIn rats, GHRP injected into a brain ventricle suppressed growth hormone rises after a GHRH analog.
Peptide
GHRP-6
Outcome
DecreaseGHRH-analog-induced GH rise
Study
ratn: not reported in source
conscious freely moving male rats
Outcome group
Growth hormone and IGF-1 · Endocrine and hormones
Studied dose
Dose not stated · intracerebroventricularAs given in this study (rat).
Source sentences
Summary
An intracerebroventricular injection of GHRP also suppressed plasma GH rises after an intravenous injection of [D-Ala2, Nle27] human GHRH (1-28)agmatine (hGHRH analog), but the GH suppression by intracerebroventricular GHRP was significantly blunted in rats treated with antirat SRIF gamma-globulin (SRIF-ab).
1 context sentence(design, population, supporting numbers)
Summary · context
The present study was designed to examine the effects of intracerebroventricular injection of His-D-Trp-Ala-Trp-D-Phe-Lys-NH2 (GHRP) on GH secretion in freely moving conscious male rats and to determine whether the central action of GHRP is mediated by increased GHRH and/or somatostatin (SRIF) release.
Report a problemFND-0905378502
03AnimalAnimal study (in vivo)Mechanism
In GHRH-antibody-treated rats, intracerebroventricular GHRP still suppressed GH release induced by GHRH analog.
In plain wordsIn rats treated with GHRH antibody, GHRP injected into a brain ventricle still suppressed growth hormone release after GHRH analog.
Peptide
GHRP-6
Outcome
DecreaseGHRH-analog-induced GH release with GHRH antibody
Study
ratn: not reported in source
conscious freely moving male rats
Outcome group
Growth hormone and IGF-1 · Endocrine and hormones
Studied dose
Dose not stated · intracerebroventricularAs given in this study (rat).
Source sentences
Summary
In addition, the suppression by intracerebroventricular GHRP of hGHRH-analog-induced GH release was observed even in rats treated with anti-rat GHRH goat gamma-globulin (GHRH-ab) which does not cross-react with hGHRH analog,
1 context sentence(design, population, supporting numbers)
Summary · context
The present study was designed to examine the effects of intracerebroventricular injection of His-D-Trp-Ala-Trp-D-Phe-Lys-NH2 (GHRP) on GH secretion in freely moving conscious male rats and to determine whether the central action of GHRP is mediated by increased GHRH and/or somatostatin (SRIF) release.
Report a problemFND-0905378504
04AnimalAnimal study (in vivo)Mechanism
In SRIF-antibody-treated rats, intracerebroventricular GHRP inhibited natural GH secretory surges.
In plain wordsIn rats treated with somatostatin antibody, GHRP injected into a brain ventricle inhibited natural growth hormone surges.
Peptide
GHRP-6
Outcome
DecreaseNatural GH surges with SRIF antibody
Study
ratn: not reported in source
conscious freely moving male rats
Outcome group
Growth hormone and IGF-1 · Endocrine and hormones
Studied dose
Dose not stated · intracerebroventricularAs given in this study (rat).
Source sentences
Summary
Furthermore, intracerebroventricularly injected GHRP was effective enough to inhibit the central-nervous-system (endogenous GHRH)-driven natural GH-secretory surges in SRIF-ab-treated rats.
1 context sentence(design, population, supporting numbers)
Summary · context
The present study was designed to examine the effects of intracerebroventricular injection of His-D-Trp-Ala-Trp-D-Phe-Lys-NH2 (GHRP) on GH secretion in freely moving conscious male rats and to determine whether the central action of GHRP is mediated by increased GHRH and/or somatostatin (SRIF) release.
Report a problemFND-0905378506
05AnimalAnimal study (in vivo)Mechanism
In control rats, intravenous GHRP increased GH during secretory surges, but responses were frequently absent during troughs.
In plain wordsIn rats, intravenous GHRP increased growth hormone during natural surges, but often produced no response during troughs.
Peptide
GHRP-6
Outcome
MixedGH after repeated intravenous GHRP
Study
ratn: not reported in source
conscious freely moving male rats
Outcome group
Growth hormone and IGF-1 · Endocrine and hormones
Studied dose
10 mu g/kg every 1 h · intravenous · 7-hour observationAs given in this study (rat).
Source sentences
Summary
When 10 mu g/kg GHRP was injected intravenously every 1 h during a 7-hour observation period in control rats without antibody treatment, plasma GH levels were increased with the peak 10 min after each injection during the surge period of the GH-secretory rhythm, but the plasma GH response to GHRP wa…
1 context sentence(design, population, supporting numbers)
Summary · context
The present study was designed to examine the effects of intracerebroventricular injection of His-D-Trp-Ala-Trp-D-Phe-Lys-NH2 (GHRP) on GH secretion in freely moving conscious male rats and to determine whether the central action of GHRP is mediated by increased GHRH and/or somatostatin (SRIF) release.
Report a problemFND-0905378507

Studies that did not test the peptide alone9

In this study, GHRP-6 was a lab tool or a carrier, part of a mix, or the subject of a lab method. These results are not evidence about taking GHRP-6.

06AnimalNot a test of GHRP-6 aloneAnimal study (in vivo)Mechanism
In rats, SRIF antibody blunted the GH suppression caused by intracerebroventricular GHRP.
In plain wordsIn rats, somatostatin antibody reduced the growth hormone suppression caused by GHRP injected into a brain ventricle.
Peptide
GHRP-6
Outcome
DecreaseGH suppression with SRIF antibody
Study
ratn: not reported in source
conscious freely moving male rats
Outcome group
Growth hormone and IGF-1 · Endocrine and hormones
Source sentences
Summary
An intracerebroventricular injection of GHRP also suppressed plasma GH rises after an intravenous injection of [D-Ala2, Nle27] human GHRH (1-28)agmatine (hGHRH analog), but the GH suppression by intracerebroventricular GHRP was significantly blunted in rats treated with antirat SRIF gamma-globulin (SRIF-ab).
1 context sentence(design, population, supporting numbers)
Summary · context
The present study was designed to examine the effects of intracerebroventricular injection of His-D-Trp-Ala-Trp-D-Phe-Lys-NH2 (GHRP) on GH secretion in freely moving conscious male rats and to determine whether the central action of GHRP is mediated by increased GHRH and/or somatostatin (SRIF) release.
In this study, GHRP-6 was a lab tool or a carrier, part of a mix, or the subject of a lab method. This result is not evidence about taking GHRP-6.
Report a problemFND-0905378503
07AnimalNot a test of GHRP-6 aloneAnimal study (in vivo)Mechanism
In rats, simultaneous SRIF and GHRH antibody treatment attenuated intracerebroventricular GHRP suppression of GHRH-analog-induced GH release.
In plain wordsIn rats, both antibodies reduced the growth hormone suppression caused by GHRP injected into a brain ventricle.
Peptide
GHRP-6
Outcome
DecreaseGH suppression with both antibodies
Study
ratn: not reported in source
conscious freely moving male rats
Outcome group
Growth hormone and IGF-1 · Endocrine and hormones
Source sentences
Summary
and again its suppression was significantly attenuated by simultaneous treatment with SRIF-ab and GHRH-ab.
1 context sentence(design, population, supporting numbers)
Summary · context
The present study was designed to examine the effects of intracerebroventricular injection of His-D-Trp-Ala-Trp-D-Phe-Lys-NH2 (GHRP) on GH secretion in freely moving conscious male rats and to determine whether the central action of GHRP is mediated by increased GHRH and/or somatostatin (SRIF) release.
In this study, GHRP-6 was a lab tool or a carrier, part of a mix, or the subject of a lab method. This result is not evidence about taking GHRP-6.
Report a problemFND-0905378505
08AnimalNot a test of GHRP-6 aloneAnimal study (in vivo)Mechanism
In rats receiving GHRP, SRIF antibody markedly elevated basal GH levels.
In plain wordsIn rats receiving GHRP, somatostatin antibody markedly increased basal growth hormone levels.
Peptide
GHRP-6
Outcome
IncreaseBasal GH with SRIF antibody
Study
ratn: not reported in source
conscious freely moving male rats
Outcome group
Growth hormone and IGF-1 · Endocrine and hormones
Source sentences
Summary
In addition, in SRIF-ab-treated rats, basal GH levels as well as plasma GH peaks after GHRP injections were markedly elevated and the obvious plasma GH rises after GHRP could be observed even during the trough period.
1 context sentence(design, population, supporting numbers)
Summary · context
The present study was designed to examine the effects of intracerebroventricular injection of His-D-Trp-Ala-Trp-D-Phe-Lys-NH2 (GHRP) on GH secretion in freely moving conscious male rats and to determine whether the central action of GHRP is mediated by increased GHRH and/or somatostatin (SRIF) release.
In this study, GHRP-6 was a lab tool or a carrier, part of a mix, or the subject of a lab method. This result is not evidence about taking GHRP-6.
Report a problemFND-0905378508
09AnimalNot a test of GHRP-6 aloneAnimal study (in vivo)Mechanism
In rats, SRIF antibody elevated GH peaks after GHRP and permitted obvious GH rises even during troughs.
In plain wordsIn rats, somatostatin antibody increased growth hormone peaks after GHRP and permitted rises even during natural troughs.
Peptide
GHRP-6
Outcome
IncreaseGH peaks with SRIF antibody
Study
ratn: not reported in source
conscious freely moving male rats
Outcome group
Growth hormone and IGF-1 · Endocrine and hormones
Source sentences
Summary
In addition, in SRIF-ab-treated rats, basal GH levels as well as plasma GH peaks after GHRP injections were markedly elevated and the obvious plasma GH rises after GHRP could be observed even during the trough period.
1 context sentence(design, population, supporting numbers)
Summary · context
The present study was designed to examine the effects of intracerebroventricular injection of His-D-Trp-Ala-Trp-D-Phe-Lys-NH2 (GHRP) on GH secretion in freely moving conscious male rats and to determine whether the central action of GHRP is mediated by increased GHRH and/or somatostatin (SRIF) release.
In this study, GHRP-6 was a lab tool or a carrier, part of a mix, or the subject of a lab method. This result is not evidence about taking GHRP-6.
Report a problemFND-0905378509
10AnimalNot a test of GHRP-6 aloneAnimal study (in vivo)Mechanism
In rats receiving GHRP, GHRH antibody lowered basal GH compared with controls.
In plain wordsIn rats receiving GHRP, GHRH antibody lowered basal growth hormone compared with controls.
Peptide
GHRP-6
Outcome
DecreaseBasal GH with GHRH antibody
Study
ratn: not reported in source
conscious freely moving male rats
Comparator
control rats
Outcome group
Growth hormone and IGF-1 · Endocrine and hormones
Source sentences
Summary
In GHRH-ab-treated rats, basal GH levels were lower, and the plasma GH response to GHRP was significantly attenuated as compared with those in control rats.
1 context sentence(design, population, supporting numbers)
Summary · context
The present study was designed to examine the effects of intracerebroventricular injection of His-D-Trp-Ala-Trp-D-Phe-Lys-NH2 (GHRP) on GH secretion in freely moving conscious male rats and to determine whether the central action of GHRP is mediated by increased GHRH and/or somatostatin (SRIF) release.
In this study, GHRP-6 was a lab tool or a carrier, part of a mix, or the subject of a lab method. This result is not evidence about taking GHRP-6.
Report a problemFND-0905378510
11AnimalNot a test of GHRP-6 aloneAnimal study (in vivo)Mechanism
In rats, GHRH antibody attenuated the GH response to GHRP compared with controls.
In plain wordsIn rats, GHRH antibody reduced the growth hormone response to GHRP compared with controls.
Peptide
GHRP-6
Outcome
DecreaseGH response with GHRH antibody
Study
ratn: not reported in source
conscious freely moving male rats
Comparator
control rats
Outcome group
Growth hormone and IGF-1 · Endocrine and hormones
Source sentences
Summary
In GHRH-ab-treated rats, basal GH levels were lower, and the plasma GH response to GHRP was significantly attenuated as compared with those in control rats.
1 context sentence(design, population, supporting numbers)
Summary · context
The present study was designed to examine the effects of intracerebroventricular injection of His-D-Trp-Ala-Trp-D-Phe-Lys-NH2 (GHRP) on GH secretion in freely moving conscious male rats and to determine whether the central action of GHRP is mediated by increased GHRH and/or somatostatin (SRIF) release.
In this study, GHRP-6 was a lab tool or a carrier, part of a mix, or the subject of a lab method. This result is not evidence about taking GHRP-6.
Report a problemFND-0905378511
12AnimalNot a test of GHRP-6 aloneAnimal study (in vivo)Mechanism
In rats, combined SRIF and GHRH antibodies produced higher GH peaks after GHRP than GHRH antibody alone.
In plain wordsIn rats, both antibodies produced higher growth hormone peaks after GHRP than GHRH antibody alone.
Peptide
GHRP-6
Outcome
IncreaseGH peaks with both antibodies
Study
ratn: not reported in source
conscious freely moving male rats
Comparator
GHRH antibody alone
Outcome group
Growth hormone and IGF-1 · Endocrine and hormones
Source sentences
Summary
Plasma GH peaks after GHRP injections in rats simultaneously treated with SRIF-ab and GHRH-ab were higher than those in rats given GHRH-ab alone,
1 context sentence(design, population, supporting numbers)
Summary · context
The present study was designed to examine the effects of intracerebroventricular injection of His-D-Trp-Ala-Trp-D-Phe-Lys-NH2 (GHRP) on GH secretion in freely moving conscious male rats and to determine whether the central action of GHRP is mediated by increased GHRH and/or somatostatin (SRIF) release.
In this study, GHRP-6 was a lab tool or a carrier, part of a mix, or the subject of a lab method. This result is not evidence about taking GHRP-6.
Report a problemFND-0905378512
13AnimalNot a test of GHRP-6 aloneAnimal study (in vivo)Mechanism
In rats, the GH response difference between combined antibodies and GHRH antibody alone was smaller than between SRIF antibody and controls.
In plain wordsIn rats, the response difference with both antibodies versus GHRH antibody alone was smaller than with somatostatin antibody versus controls.
Peptide
GHRP-6
Outcome
DecreaseRelative antibody effect on GH response
Study
ratn: not reported in source
conscious freely moving male rats
Outcome group
Growth hormone and IGF-1 · Endocrine and hormones
Source sentences
Summary
but its difference in GH response was obviously smaller than that observed between SRIF-ab-treated and control rats,
1 context sentence(design, population, supporting numbers)
Summary · context
The present study was designed to examine the effects of intracerebroventricular injection of His-D-Trp-Ala-Trp-D-Phe-Lys-NH2 (GHRP) on GH secretion in freely moving conscious male rats and to determine whether the central action of GHRP is mediated by increased GHRH and/or somatostatin (SRIF) release.
In this study, GHRP-6 was a lab tool or a carrier, part of a mix, or the subject of a lab method. This result is not evidence about taking GHRP-6.
Report a problemFND-0905378513
14AnimalNot a test of GHRP-6 aloneAnimal study (in vivo)Mechanism
In antibody-treated rats, combined GHRH analog and GHRP produced GH peaks greater than the sum of peaks after either alone.
In plain wordsIn rats treated with antibodies, combined GHRH analog and GHRP produced growth hormone peaks greater than the sum after separate injections.
Peptide
GHRP-6
Outcome
IncreaseSynergistic GH peaks with GHRH analog
Study
ratn: not reported in source
conscious freely moving male rats
Comparator
sum of separate peptide GH peaks
Outcome group
Growth hormone and IGF-1 · Endocrine and hormones
Source sentences
Summary
The peak GH values after simultaneous injection of hGHRH analog and GHRP were significantly higher than the sum of GH peaks after each injection of hGHRH analog and GHRP.
2 context sentences(design, population, supporting numbers)
Summary · context
To further clarify the interaction of GHRH and GHRP in GH release, hGHRH analog was injected intravenously alone or simultaneously with GHRP in rats treated with SRIF-ab and GHRH-ab.
Summary · context
The present study was designed to examine the effects of intracerebroventricular injection of His-D-Trp-Ala-Trp-D-Phe-Lys-NH2 (GHRP) on GH secretion in freely moving conscious male rats and to determine whether the central action of GHRP is mediated by increased GHRH and/or somatostatin (SRIF) release.
In this study, GHRP-6 was a lab tool or a carrier, part of a mix, or the subject of a lab method. This result is not evidence about taking GHRP-6.
Report a problemFND-0905378514

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Version history

PepBank keeps every version of the data on this study. Status changes from Retraction Watch, Europe PMC and Crossref are listed with their source.

Version 2Current
21 Oct 2026, 15:58 UTC
Study results updated
Version 1
9 Oct 2026, 03:13 UTC
First added · source update from Europe PMC

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