Molecular cloning and functional characterization of the oxytocin receptor from a rat pancreatic cell line (RINm5F).

Jeng YJ, Lolait SJ, Strakova Z, Chen C, Copland JA, Mellman D, Hellmich MR, Soloff MS

Neuropeptides1 Dec 1996Cited by 22 (Europe PMC)

PMID9004255DOI10.1016/s0143-4179(96)90039-6PepBankPUB-09004255

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Summary

The text of this summary is not shown here, because the article license does not allow reuse. Read it at the source (opens in a new tab)

Text rights: Publisher copyright. Results still quote short attributed sentences.

Results from this study4

4 results from studies that gave the peptide. No result from this study comes from people.

01LabIn vitro (cells)Mechanism
In CHO cells expressing the cloned rat pancreatic cell line oxytocin receptor, oxytocin increased cytosolic calcium from intracellular and extracellular sources.
In plain wordsIn cells carrying the oxytocin receptor, oxytocin raised calcium levels inside the cells.
Peptide
Oxytocin
Outcome
IncreaseCytosolic calcium concentration
Study
n: not reported in source
CHO cells stably transfected with the RINm5F rat oxytocin receptor (CHO-OTR)
Outcome group
Receptor activity and mechanism · Pharmacology
Studied dose
Dose not stated
Source sentences
Summary
Stimulation of CHO-OTR cells with OT caused an increase in cytosolic calcium concentration originating from both intracellular and extracellular sources
1 context sentence(design, population, supporting numbers)
Summary · context
After the RIN cell OTR cDNA was stably transfected into CHO cells (CHO-OTR), the cell membranes bound iodinated oxytocin antagonist with an apparent Kd comparable to that of RIN cell membranes and those from other OT target cells.
Report a problemFND-0900425501
02LabIn vitro (cells)Mechanism
In CHO cells expressing the cloned rat pancreatic cell line oxytocin receptor, oxytocin caused a dose-dependent increase in inositol phosphate levels.
In plain wordsIn cells carrying the oxytocin receptor, oxytocin raised inositol phosphate levels as the dose rose.
Peptide
Oxytocin
Outcome
IncreaseInositol phosphate levels
Study
n: not reported in source
CHO cells stably transfected with the RINm5F rat oxytocin receptor (CHO-OTR)
Outcome group
Receptor activity and mechanism · Pharmacology
Studied dose
Dose not stated
Source sentences
Summary
a dose-dependent increase in inositol phosphate levels.
1 context sentence(design, population, supporting numbers)
Summary · context
After the RIN cell OTR cDNA was stably transfected into CHO cells (CHO-OTR), the cell membranes bound iodinated oxytocin antagonist with an apparent Kd comparable to that of RIN cell membranes and those from other OT target cells.
Report a problemFND-0900425502
03LabIn vitro (cells)Mechanism
In CHO cells expressing the cloned rat pancreatic cell line oxytocin receptor, oxytocin stimulated arachidonic acid release.
In plain wordsIn cells carrying the oxytocin receptor, oxytocin triggered release of arachidonic acid.
Peptide
Oxytocin
Outcome
IncreaseArachidonic acid release
Study
n: not reported in source
CHO cells stably transfected with the RINm5F rat oxytocin receptor (CHO-OTR)
Outcome group
Receptor activity and mechanism · Pharmacology
Studied dose
Dose not stated
Source sentences
Summary
Arachidonic acid release and PGE2 synthesis were also stimulated by OT.
1 context sentence(design, population, supporting numbers)
Summary · context
After the RIN cell OTR cDNA was stably transfected into CHO cells (CHO-OTR), the cell membranes bound iodinated oxytocin antagonist with an apparent Kd comparable to that of RIN cell membranes and those from other OT target cells.
Report a problemFND-0900425503
04LabIn vitro (cells)Mechanism
In CHO cells expressing the cloned rat pancreatic cell line oxytocin receptor, oxytocin stimulated PGE2 synthesis.
In plain wordsIn cells carrying the oxytocin receptor, oxytocin triggered production of PGE2.
Peptide
Oxytocin
Outcome
IncreasePGE2 synthesis
Study
n: not reported in source
CHO cells stably transfected with the RINm5F rat oxytocin receptor (CHO-OTR)
Outcome group
Receptor activity and mechanism · Pharmacology
Studied dose
Dose not stated
Source sentences
Summary
Arachidonic acid release and PGE2 synthesis were also stimulated by OT.
1 context sentence(design, population, supporting numbers)
Summary · context
After the RIN cell OTR cDNA was stably transfected into CHO cells (CHO-OTR), the cell membranes bound iodinated oxytocin antagonist with an apparent Kd comparable to that of RIN cell membranes and those from other OT target cells.
Report a problemFND-0900425504

AI wrote and checked these results. AI can make mistakes, so check important results against the source.

Version history

PepBank keeps every version of the data on this study. Status changes from Retraction Watch, Europe PMC and Crossref are listed with their source.

Version 2Current
11 Oct 2026, 20:03 UTC
Study results updated
Version 1
9 Oct 2026, 02:52 UTC
First added · source update from Europe PMC

Subjects

Terms as the source lists them.

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