Growth hormone (GH)-releasing peptide and GH releasing hormone stimulate GH release from subpopulations of somatotrophs in rats.

Mitani M, Kaji H, Abe H, Chihara K

Journal of neuroendocrinology1 Nov 1996Cited by 5 (Europe PMC)

PMID8933359DOI10.1046/j.1365-2826.1996.05283.xPepBankPUB-08933359

Publisher page PubMedAsk about this study

Summary

The text of this summary is not shown here, because the article license does not allow reuse. Read it at the source (opens in a new tab)

Text rights: Publisher copyright. Results still quote short attributed sentences.

Results from this study11

4 results from studies that gave the peptide. No result from this study comes from people.

01LabIn vitro (cells)Mechanism
In rat pituitary cells, GHRP‑6 increased plaque forming cells versus vehicle.
In plain wordsIn cells from rats, GHRP‑6 increased plaque forming cells compared with vehicle.
Peptide
GHRP-6
Outcome
IncreasePlaque forming cells
Study
ratn: not reported in source
dispersed anterior pituitary cells
Comparator
vehicle
Outcome group
Growth hormone and IGF-1 · Endocrine and hormones
Studied dose
100 nM · 30 minAs given in this study (rat).
Measurements
Plaque forming cells
29.4%
GHRP-6
Plaque forming cells
24.3%
vehicle
Source sentences
Summary
10 nM GHRH and 100 nM GHRP-6 each caused a significant increase in % PFC (%) (GHRH 39.15, GHRP-6 29.4, vs vehicle 24.3, P < 0.01), MPA (x 10(-2) microns2) (GHRH 124.04, GHRP-6 94.80, vs vehicle 44.57, P < 0.01) and TSI (x 10(-2)) (GHRP-6 32.87, vs vehicle 10.84, P < 0.01).
2 context sentences(design, population, supporting numbers)
Summary · context
Using reverse hemolytic plaque assay (RHPA), we have compared the effects of human GHRH and GHRP-6 on GH release from the dispersed individual cells of rat anterior pituitary.
Summary · context
In a single RHPA, we quantified the percentage of plaque forming cells (% PFC) and their mean plaque area (MPA) after 30 min-incubation, and calculated a total secretion index (TSI) by multiplying % PFC and MPA.
Report a problemFND-0893335901
02LabIn vitro (cells)Mechanism
In rat pituitary cells, GHRP‑6 increased mean plaque area versus vehicle.
In plain wordsIn cells from rats, GHRP‑6 increased mean plaque area compared with vehicle.
Peptide
GHRP-6
Outcome
IncreaseMean plaque area
Study
ratn: not reported in source
dispersed anterior pituitary cells
Comparator
vehicle
Outcome group
Growth hormone and IGF-1 · Endocrine and hormones
Studied dose
100 nM · 30 minAs given in this study (rat).
Source sentences
Summary
10 nM GHRH and 100 nM GHRP-6 each caused a significant increase in % PFC (%) (GHRH 39.15, GHRP-6 29.4, vs vehicle 24.3, P < 0.01), MPA (x 10(-2) microns2) (GHRH 124.04, GHRP-6 94.80, vs vehicle 44.57, P < 0.01) and TSI (x 10(-2)) (GHRP-6 32.87, vs vehicle 10.84, P < 0.01).
2 context sentences(design, population, supporting numbers)
Summary · context
Using reverse hemolytic plaque assay (RHPA), we have compared the effects of human GHRH and GHRP-6 on GH release from the dispersed individual cells of rat anterior pituitary.
Summary · context
In a single RHPA, we quantified the percentage of plaque forming cells (% PFC) and their mean plaque area (MPA) after 30 min-incubation, and calculated a total secretion index (TSI) by multiplying % PFC and MPA.
Report a problemFND-0893335902
03LabIn vitro (cells)Mechanism
In rat pituitary cells, GHRP‑6 increased total secretion index versus vehicle.
In plain wordsIn cells from rats, GHRP‑6 increased total secretion index compared with vehicle.
Peptide
GHRP-6
Outcome
IncreaseTotal secretion index
Study
ratn: not reported in source
dispersed anterior pituitary cells
Comparator
vehicle
Outcome group
Growth hormone and IGF-1 · Endocrine and hormones
Studied dose
100 nM · 30 minAs given in this study (rat).
Source sentences
Summary
10 nM GHRH and 100 nM GHRP-6 each caused a significant increase in % PFC (%) (GHRH 39.15, GHRP-6 29.4, vs vehicle 24.3, P < 0.01), MPA (x 10(-2) microns2) (GHRH 124.04, GHRP-6 94.80, vs vehicle 44.57, P < 0.01) and TSI (x 10(-2)) (GHRP-6 32.87, vs vehicle 10.84, P < 0.01).
2 context sentences(design, population, supporting numbers)
Summary · context
Using reverse hemolytic plaque assay (RHPA), we have compared the effects of human GHRH and GHRP-6 on GH release from the dispersed individual cells of rat anterior pituitary.
Summary · context
In a single RHPA, we quantified the percentage of plaque forming cells (% PFC) and their mean plaque area (MPA) after 30 min-incubation, and calculated a total secretion index (TSI) by multiplying % PFC and MPA.
Report a problemFND-0893335903
04LabIn vitro (cells)Mechanism
In rat pituitary cells, cells responsive only to GHRP‑6 comprised 11.9% versus 6.1% in controls.
In plain wordsIn cells from rats, cells responsive only to GHRP‑6 comprised 11.9% compared with 6.1% in controls.
Peptide
GHRP-6
Outcome
IncreaseCells responsive only to GHRP-6
Study
ratn: not reported in source
dispersed anterior pituitary cells
Comparator
control
Outcome group
Growth hormone and IGF-1 · Endocrine and hormones
Studied dose
Dose not stated
Source sentences
Summary
There were somatotrophs responsive to only GHRH (23.3% vs control 6.2%, P < 0.01), those responsive to only GHRP-6 (11.9% vs control 6.1%, P < 0.01), and those responsive to both GHRH and GHRP-6 (7.8% vs control 0.2%, P < 0.01).
2 context sentences(design, population, supporting numbers)
Summary · context
Using reverse hemolytic plaque assay (RHPA), we have compared the effects of human GHRH and GHRP-6 on GH release from the dispersed individual cells of rat anterior pituitary.
Summary · context
In a single RHPA, we quantified the percentage of plaque forming cells (% PFC) and their mean plaque area (MPA) after 30 min-incubation, and calculated a total secretion index (TSI) by multiplying % PFC and MPA.
Report a problemFND-0893335910

Studies that did not test the peptide alone7

In this study, GHRP-6 was a lab tool or a carrier, part of a mix, or the subject of a lab method. These results are not evidence about taking GHRP-6.

05LabNot a test of GHRP-6 aloneIn vitro (cells)Mechanism
In rat pituitary cells, GHRP‑6 with GHRH increased plaque forming cells versus vehicle.
In plain wordsIn cells from rats, GHRP‑6 with GHRH increased plaque forming cells compared with vehicle.
Peptide
GHRP-6
Outcome
IncreaseCombined treatment plaque forming cells
Study
ratn: not reported in source
dispersed anterior pituitary cells
Comparator
vehicle
Outcome group
Growth hormone and IGF-1 · Endocrine and hormones
Source sentences
Summary
Simultaneous addition of both secretagogues caused a further increase in GH release (%PFC 46.4, MPA 142.55, TSI 69.82, P < 0.01 vs vehicle), although the effect was additive but not synergistic.
2 context sentences(design, population, supporting numbers)
Summary · context
Using reverse hemolytic plaque assay (RHPA), we have compared the effects of human GHRH and GHRP-6 on GH release from the dispersed individual cells of rat anterior pituitary.
Summary · context
In a single RHPA, we quantified the percentage of plaque forming cells (% PFC) and their mean plaque area (MPA) after 30 min-incubation, and calculated a total secretion index (TSI) by multiplying % PFC and MPA.
In this study, GHRP-6 was a lab tool or a carrier, part of a mix, or the subject of a lab method. This result is not evidence about taking GHRP-6.
Report a problemFND-0893335904
06LabNot a test of GHRP-6 aloneIn vitro (cells)Mechanism
In rat pituitary cells, GHRP‑6 with GHRH increased mean plaque area versus vehicle.
In plain wordsIn cells from rats, GHRP‑6 with GHRH increased mean plaque area compared with vehicle.
Peptide
GHRP-6
Outcome
IncreaseCombined treatment mean plaque area
Study
ratn: not reported in source
dispersed anterior pituitary cells
Comparator
vehicle
Outcome group
Growth hormone and IGF-1 · Endocrine and hormones
Source sentences
Summary
Simultaneous addition of both secretagogues caused a further increase in GH release (%PFC 46.4, MPA 142.55, TSI 69.82, P < 0.01 vs vehicle), although the effect was additive but not synergistic.
2 context sentences(design, population, supporting numbers)
Summary · context
Using reverse hemolytic plaque assay (RHPA), we have compared the effects of human GHRH and GHRP-6 on GH release from the dispersed individual cells of rat anterior pituitary.
Summary · context
In a single RHPA, we quantified the percentage of plaque forming cells (% PFC) and their mean plaque area (MPA) after 30 min-incubation, and calculated a total secretion index (TSI) by multiplying % PFC and MPA.
In this study, GHRP-6 was a lab tool or a carrier, part of a mix, or the subject of a lab method. This result is not evidence about taking GHRP-6.
Report a problemFND-0893335905
07LabNot a test of GHRP-6 aloneIn vitro (cells)Mechanism
In rat pituitary cells, GHRP‑6 with GHRH increased total secretion index versus vehicle, with additive rather than synergistic effects.
In plain wordsIn cells from rats, GHRP‑6 with GHRH increased total secretion index compared with vehicle. The effect was additive.
Peptide
GHRP-6
Outcome
IncreaseCombined treatment total secretion index
Study
ratn: not reported in source
dispersed anterior pituitary cells
Comparator
vehicle
Outcome group
Growth hormone and IGF-1 · Endocrine and hormones
Source sentences
Summary
Simultaneous addition of both secretagogues caused a further increase in GH release (%PFC 46.4, MPA 142.55, TSI 69.82, P < 0.01 vs vehicle), although the effect was additive but not synergistic.
2 context sentences(design, population, supporting numbers)
Summary · context
Using reverse hemolytic plaque assay (RHPA), we have compared the effects of human GHRH and GHRP-6 on GH release from the dispersed individual cells of rat anterior pituitary.
Summary · context
In a single RHPA, we quantified the percentage of plaque forming cells (% PFC) and their mean plaque area (MPA) after 30 min-incubation, and calculated a total secretion index (TSI) by multiplying % PFC and MPA.
In this study, GHRP-6 was a lab tool or a carrier, part of a mix, or the subject of a lab method. This result is not evidence about taking GHRP-6.
Report a problemFND-0893335906
08LabNot a test of GHRP-6 aloneIn vitro (cells)Mechanism
In rat pituitary cells, SMS partially suppressed plaque forming cells after GHRP‑6 and/or GHRH stimulation.
In plain wordsIn cells from rats, SMS partially suppressed plaque forming cells after GHRP‑6 and/or GHRH stimulation.
Peptide
GHRP-6
Outcome
DecreasePlaque forming cells with SMS
Study
ratn: not reported in source
dispersed anterior pituitary cells
Outcome group
Growth hormone and IGF-1 · Endocrine and hormones
Source sentences
Summary
Somatostatin analog, SMS201-995 (SMS) partially suppressed all parameters in GH secretion after stimulation by GHRH and/or GHRP-6.
2 context sentences(design, population, supporting numbers)
Summary · context
In a single RHPA, we quantified the percentage of plaque forming cells (% PFC) and their mean plaque area (MPA) after 30 min-incubation, and calculated a total secretion index (TSI) by multiplying % PFC and MPA.
Summary · context
Using reverse hemolytic plaque assay (RHPA), we have compared the effects of human GHRH and GHRP-6 on GH release from the dispersed individual cells of rat anterior pituitary.
In this study, GHRP-6 was a lab tool or a carrier, part of a mix, or the subject of a lab method. This result is not evidence about taking GHRP-6.
Report a problemFND-0893335907
09LabNot a test of GHRP-6 aloneIn vitro (cells)Mechanism
In rat pituitary cells, SMS partially suppressed mean plaque area after GHRP‑6 and/or GHRH stimulation.
In plain wordsIn cells from rats, SMS partially suppressed mean plaque area after GHRP‑6 and/or GHRH stimulation.
Peptide
GHRP-6
Outcome
DecreaseMean plaque area with SMS
Study
ratn: not reported in source
dispersed anterior pituitary cells
Outcome group
Growth hormone and IGF-1 · Endocrine and hormones
Source sentences
Summary
Somatostatin analog, SMS201-995 (SMS) partially suppressed all parameters in GH secretion after stimulation by GHRH and/or GHRP-6.
2 context sentences(design, population, supporting numbers)
Summary · context
In a single RHPA, we quantified the percentage of plaque forming cells (% PFC) and their mean plaque area (MPA) after 30 min-incubation, and calculated a total secretion index (TSI) by multiplying % PFC and MPA.
Summary · context
Using reverse hemolytic plaque assay (RHPA), we have compared the effects of human GHRH and GHRP-6 on GH release from the dispersed individual cells of rat anterior pituitary.
In this study, GHRP-6 was a lab tool or a carrier, part of a mix, or the subject of a lab method. This result is not evidence about taking GHRP-6.
Report a problemFND-0893335908
10LabNot a test of GHRP-6 aloneIn vitro (cells)Mechanism
In rat pituitary cells, SMS partially suppressed total secretion index after GHRP‑6 and/or GHRH stimulation.
In plain wordsIn cells from rats, SMS partially suppressed total secretion index after GHRP‑6 and/or GHRH stimulation.
Peptide
GHRP-6
Outcome
DecreaseTotal secretion index with SMS
Study
ratn: not reported in source
dispersed anterior pituitary cells
Outcome group
Growth hormone and IGF-1 · Endocrine and hormones
Source sentences
Summary
Somatostatin analog, SMS201-995 (SMS) partially suppressed all parameters in GH secretion after stimulation by GHRH and/or GHRP-6.
2 context sentences(design, population, supporting numbers)
Summary · context
In a single RHPA, we quantified the percentage of plaque forming cells (% PFC) and their mean plaque area (MPA) after 30 min-incubation, and calculated a total secretion index (TSI) by multiplying % PFC and MPA.
Summary · context
Using reverse hemolytic plaque assay (RHPA), we have compared the effects of human GHRH and GHRP-6 on GH release from the dispersed individual cells of rat anterior pituitary.
In this study, GHRP-6 was a lab tool or a carrier, part of a mix, or the subject of a lab method. This result is not evidence about taking GHRP-6.
Report a problemFND-0893335909
11LabNot a test of GHRP-6 aloneIn vitro (cells)Mechanism
In rat pituitary cells, cells responsive to both GHRH and GHRP‑6 comprised 7.8% versus 0.2% in controls.
In plain wordsIn cells from rats, cells responsive to both peptides comprised 7.8% compared with 0.2% in controls.
Peptide
GHRP-6
Outcome
IncreaseCells responsive to both secretagogues
Study
ratn: not reported in source
dispersed anterior pituitary cells
Comparator
control
Outcome group
Growth hormone and IGF-1 · Endocrine and hormones
Source sentences
Summary
There were somatotrophs responsive to only GHRH (23.3% vs control 6.2%, P < 0.01), those responsive to only GHRP-6 (11.9% vs control 6.1%, P < 0.01), and those responsive to both GHRH and GHRP-6 (7.8% vs control 0.2%, P < 0.01).
2 context sentences(design, population, supporting numbers)
Summary · context
Using reverse hemolytic plaque assay (RHPA), we have compared the effects of human GHRH and GHRP-6 on GH release from the dispersed individual cells of rat anterior pituitary.
Summary · context
In a single RHPA, we quantified the percentage of plaque forming cells (% PFC) and their mean plaque area (MPA) after 30 min-incubation, and calculated a total secretion index (TSI) by multiplying % PFC and MPA.
In this study, GHRP-6 was a lab tool or a carrier, part of a mix, or the subject of a lab method. This result is not evidence about taking GHRP-6.
Report a problemFND-0893335911

AI wrote and checked these results. AI can make mistakes, so check important results against the source.

Version history

PepBank keeps every version of the data on this study. Status changes from Retraction Watch, Europe PMC and Crossref are listed with their source.

Version 2Current
21 Oct 2026, 15:58 UTC
Study results updated
Version 1
9 Oct 2026, 03:13 UTC
First added · source update from Europe PMC

Subjects

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