Growth hormone releasing hexapeptide (GHRP-6) activates the inositol (1,4,5)-trisphosphate/diacylglycerol pathway in rat anterior pituitary cells.

Mau SE, Witt MR, Bjerrum OJ, Saermark T, Vilhardt H

Journal of receptor and signal transduction research1 Jan 1995Cited by 14 (Europe PMC)

PMID8903947DOI10.3109/10799899509045223PepBankPUB-08903947

Publisher page PubMedAsk about this study

Summary

The text of this summary is not shown here, because the article license does not allow reuse. Read it at the source (opens in a new tab)

Text rights: Publisher copyright. Results still quote short attributed sentences.

Results from this study7

5 results from studies that gave the peptide. No result from this study comes from people.

01LabIn vitro (cells)Mechanism
In cultured rat pituitary cells, GHRP‑6 caused dose-dependent GH release.
In plain wordsIn cells from rat pituitaries, GHRP‑6 increased growth hormone release as the dose increased.
Peptide
GHRP-6
Outcome
IncreaseGH release
Study
ratn: not reported in source
Cultured rat anterior pituitary cells
Outcome group
Growth hormone and IGF-1 · Endocrine and hormones
Studied dose
Dose not stated
Source sentences
Summary
Cultured pituitary cells responded upon challenge with GHRP-6 with a dose-dependent release of GH.
2 context sentences(design, population, supporting numbers)
Title · context
Growth hormone releasing hexapeptide (GHRP-6) activates the inositol (1,4,5)-trisphosphate/diacylglycerol pathway in rat anterior pituitary cells.
Summary · context
We have tested the influence of GHRP-6 on the inositol phospholipid second messenger system in cultured anterior pituitary cells.
Report a problemFND-0890394701
02LabIn vitro (cells)Mechanism
In cultured rat pituitary cells, GHRP‑6 increased labelled inositol phosphates in a dose-dependent manner.
In plain wordsIn cells from rat pituitaries, GHRP‑6 increased labelled inositol phosphates as the dose increased.
Peptide
GHRP-6
Outcome
IncreaseInositol phosphate accumulation
Study
ratn: not reported in source
Cultured rat anterior pituitary cells
Outcome group
Receptor activity and mechanism · Endocrine and hormones
Studied dose
Dose not stated
Source sentences
Summary
Moreover, incubation of GHRP-6 with pituitary cell cultures labelled with myo-[3H]inositol resulted in a dose-dependent rise in [3H]inositol phosphates.
2 context sentences(design, population, supporting numbers)
Title · context
Growth hormone releasing hexapeptide (GHRP-6) activates the inositol (1,4,5)-trisphosphate/diacylglycerol pathway in rat anterior pituitary cells.
Summary · context
We have tested the influence of GHRP-6 on the inositol phospholipid second messenger system in cultured anterior pituitary cells.
Report a problemFND-0890394702
03LabIn vitro (cells)Mechanism
In rat pituitary cells, GHRP‑6 increased MBP4-14 phosphorylation measured with cytosolic or plasma membrane fractions.
In plain wordsIn lab tests, GHRP‑6 increased phosphorylation of a protein kinase C substrate in rat pituitary cell fractions.
Peptide
GHRP-6
Outcome
IncreaseMBP4-14 phosphorylation in cell fractions
Study
ratn: not reported in source
Cultured rat anterior pituitary cells
Outcome group
Receptor activity and mechanism · Endocrine and hormones
Studied dose
Dose not stated
Source sentences
Summary
Brief stimulation of pituitary cells with GHRP-6 increased phosphorylation of MBP4-14, a specific protein kinase C substrate, when incubated with the cytosol- or plasma membrane fraction from the stimulated cells.
2 context sentences(design, population, supporting numbers)
Title · context
Growth hormone releasing hexapeptide (GHRP-6) activates the inositol (1,4,5)-trisphosphate/diacylglycerol pathway in rat anterior pituitary cells.
Summary · context
We have tested the influence of GHRP-6 on the inositol phospholipid second messenger system in cultured anterior pituitary cells.
Report a problemFND-0890394703
04LabIn vitro (cells)Mechanism
In GHRP‑6-stimulated rat pituitary cells, introduced MBP4-14 showed increased phosphorylation in permeabilized cells.
In plain wordsIn lab tests, a protein kinase C substrate showed increased phosphorylation inside permeabilized rat pituitary cells stimulated with GHRP‑6.
Peptide
GHRP-6
Outcome
IncreaseMBP4-14 phosphorylation in permeabilized cells
Study
ratn: not reported in source
Cultured rat anterior pituitary cells
Outcome group
Receptor activity and mechanism · Endocrine and hormones
Studied dose
Dose not stated
Source sentences
Summary
Furthermore, introduction of MBP4-14 into the cytosol in digitonin permeabilized pituitary cells caused increased phosphorylation of this substrate.
3 context sentences(design, population, supporting numbers)
Summary · context
Brief stimulation of pituitary cells with GHRP-6 increased phosphorylation of MBP4-14, a specific protein kinase C substrate, when incubated with the cytosol- or plasma membrane fraction from the stimulated cells.
Title · context
Growth hormone releasing hexapeptide (GHRP-6) activates the inositol (1,4,5)-trisphosphate/diacylglycerol pathway in rat anterior pituitary cells.
Summary · context
We have tested the influence of GHRP-6 on the inositol phospholipid second messenger system in cultured anterior pituitary cells.
Report a problemFND-0890394704
05LabIn vitro (cells)Mechanism
In individual rat somatotrophs, GHRP‑6 increased intracellular calcium.
In plain wordsIn cells from rat pituitaries, GHRP‑6 increased calcium inside individual growth hormone-producing cells.
Peptide
GHRP-6
Outcome
IncreaseIntracellular calcium
Study
ratn: not reported in source
Cultured rat anterior pituitary cells
Outcome group
Receptor activity and mechanism · Endocrine and hormones
Studied dose
Dose not stated
Source sentences
Summary
GHRP-6 induced a rise in intracellular Ca2+ in individual somatotrophs loaded with the Ca2+ indicator, Fura-2.
2 context sentences(design, population, supporting numbers)
Title · context
Growth hormone releasing hexapeptide (GHRP-6) activates the inositol (1,4,5)-trisphosphate/diacylglycerol pathway in rat anterior pituitary cells.
Summary · context
We have tested the influence of GHRP-6 on the inositol phospholipid second messenger system in cultured anterior pituitary cells.
Report a problemFND-0890394705

Studies that did not test the peptide alone2

In this study, GHRP-6 was a lab tool or a carrier, part of a mix, or the subject of a lab method. These results are not evidence about taking GHRP-6.

06LabNot a test of GHRP-6 aloneIn vitro (cells)Mechanism
In rat pituitary cells, somatostatin pretreatment diminished the calcium spike elicited by GHRP‑6.
In plain wordsIn cells from rat pituitaries, somatostatin pretreatment reduced the calcium spike compared with GHRP‑6 without pretreatment.
Peptide
GHRP-6
Outcome
DecreaseCalcium spike after somatostatin pretreatment
Study
ratn: not reported in source
Cultured rat anterior pituitary cells
Comparator
GHRP-6 without somatostatin pretreatment
Outcome group
Receptor activity and mechanism · Endocrine and hormones
Source sentences
Summary
Preincubation (3 min) with somatostatin (SRIF) diminished the Ca2+ spike elicited by GHRP-6, while no effect of SRIF was observed when added simultaneously with GHRP-6.
2 context sentences(design, population, supporting numbers)
Title · context
Growth hormone releasing hexapeptide (GHRP-6) activates the inositol (1,4,5)-trisphosphate/diacylglycerol pathway in rat anterior pituitary cells.
Summary · context
We have tested the influence of GHRP-6 on the inositol phospholipid second messenger system in cultured anterior pituitary cells.
In this study, GHRP-6 was a lab tool or a carrier, part of a mix, or the subject of a lab method. This result is not evidence about taking GHRP-6.
Report a problemFND-0890394706
07LabNot a test of GHRP-6 aloneIn vitro (cells)Mechanism
In rat pituitary cells, simultaneous somatostatin exposure did not affect the calcium response elicited by GHRP‑6.
In plain wordsIn cells from rat pituitaries, simultaneous somatostatin did not change the calcium response compared with GHRP‑6 without somatostatin.
Peptide
GHRP-6
Outcome
No significant changeCalcium response during simultaneous somatostatin exposure
Study
ratn: not reported in source
Cultured rat anterior pituitary cells
Comparator
GHRP-6 without simultaneous somatostatin
Outcome group
Receptor activity and mechanism · Endocrine and hormones
Source sentences
Summary
Preincubation (3 min) with somatostatin (SRIF) diminished the Ca2+ spike elicited by GHRP-6, while no effect of SRIF was observed when added simultaneously with GHRP-6.
2 context sentences(design, population, supporting numbers)
Title · context
Growth hormone releasing hexapeptide (GHRP-6) activates the inositol (1,4,5)-trisphosphate/diacylglycerol pathway in rat anterior pituitary cells.
Summary · context
We have tested the influence of GHRP-6 on the inositol phospholipid second messenger system in cultured anterior pituitary cells.
In this study, GHRP-6 was a lab tool or a carrier, part of a mix, or the subject of a lab method. This result is not evidence about taking GHRP-6.
Report a problemFND-0890394707

AI wrote and checked these results. AI can make mistakes, so check important results against the source.

Version history

PepBank keeps every version of the data on this study. Status changes from Retraction Watch, Europe PMC and Crossref are listed with their source.

Version 2Current
21 Oct 2026, 15:58 UTC
Study results updated
Version 1
9 Oct 2026, 03:13 UTC
First added · source update from Europe PMC

Subjects

Terms as the source lists them.

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