In bovine granulosa cells grown without anchorage, the IGF-I analogue (LR3IGF-I) at 100 ng ml-1 significantly increased DNA synthesis.
In plain wordsIn cow granulosa cells in lab culture, the IGF-I analogue LR3IGF-I increased DNA synthesis.
- Peptide
- IGF-1 LR3
- Outcome
- IncreaseDNA synthesis ([3H]thymidine incorporation)
- Study
bovinen = 5
bovine granulosa cells in soft agar-methylcellulose culture- Outcome group
- Cell proliferation and differentiation · Reproductive and sexual health
- Studied dose
- 100 ng ml-1As given in this study (bovine).
Source sentences
Both IGF-I (100 ng ml-1) and IGF-II (100 ng ml-1) and their respective analogues, as well as insulin, all at the same concentration, significantly increased DNA synthesis as determined by [3H]thymidine incorporation (n = 5).
2 context sentences(design, population, supporting numbers)
but not that stimulated by the analogue, LR3IGF-I, which has low affinity for IGFBPs.
The effects of insulin-like growth factors (IGFs) and insulin-like growth factor binding protein 1 (IGFBP-1) on the replication of such cells was investigated by isolating granulosa cells from follicles (3-5 mm diameter) from cyclic cows and culturing them in soft agar-methylcellulose solution.