The effects of GH-releasing peptide-6 (GHRP-6) and GHRP-2 on intracellular adenosine 3',5'-monophosphate (cAMP) levels and GH secretion in ovine and rat somatotrophs.

Wu D, Chen C, Zhang J, Bowers CY, Clarke IJ

The Journal of endocrinology1 Feb 1996Cited by 34 (Europe PMC)

PMID8699133DOI10.1677/joe.0.1480197PepBankPUB-08699133

Publisher page PubMedAsk about this study

Summary

The text of this summary is not shown here, because the article license does not allow reuse. Read it at the source (opens in a new tab)

Text rights: Publisher copyright. Results still quote short attributed sentences.

Results from this study26

13 results from studies that gave the peptide. No result from this study comes from people.

01LabIn vitro (cells)Activity measurement
In ovine somatotrophs, GHRP‑6 caused GH release without an increase in intracellular cAMP.
In plain wordsIn lab tests on sheep pituitary cells, GHRP‑6 caused growth hormone release.
Peptide
GHRP-6
Outcome
IncreaseGH release
Study
sheepn: not reported in source
partially purified ovine somatotrophs
Outcome group
Receptor activity and mechanism · Endocrine and hormones
Studied dose
Dose not stated
Source sentences
Summary
GHRP-6 caused GH release without an increase in intracellular cAMP levels.
1 context sentence(design, population, supporting numbers)
Summary · context
The mechanism of action of GH-releasing peptide-6 (GHRP-6) and GHRP-2 on GH release was investigated in ovine and rat pituitary cells in vitro.
Report a problemFND-0869913301
02LabIn vitro (cells)Mechanism
In ovine somatotrophs, GHRP‑6 did not increase intracellular cAMP concentrations.
In plain wordsIn lab tests on sheep pituitary cells, GHRP‑6 did not raise cAMP, a cell signal.
Peptide
GHRP-6
Outcome
No significant changeIntracellular cAMP
Study
sheepn: not reported in source
partially purified ovine somatotrophs
Outcome group
Receptor activity and mechanism · Endocrine and hormones
Studied dose
Dose not stated
Source sentences
Summary
GHRP-6 did not increase cAMP levels.
1 context sentence(design, population, supporting numbers)
Summary · context
The mechanism of action of GH-releasing peptide-6 (GHRP-6) and GHRP-2 on GH release was investigated in ovine and rat pituitary cells in vitro.
Report a problemFND-0869913302
03LabIn vitro (cells)Mechanism
In ovine somatotrophs, the cAMP antagonist Rp-cAMP did not prevent the GH release caused by GHRP‑6.
In plain wordsIn lab tests on sheep pituitary cells, blocking cAMP did not stop growth hormone release caused by GHRP‑6.
Peptide
GHRP-6
Outcome
No significant changeGH release with cAMP antagonist Rp-cAMP
Study
sheepn: not reported in source
partially purified ovine somatotrophs
Outcome group
Receptor activity and mechanism · Endocrine and hormones
Studied dose
Dose not stated
Source sentences
Summary
The cAMP antagonist did not prevent the effect of GHRP-6 on GH secretion
1 context sentence(design, population, supporting numbers)
Summary · context
The mechanism of action of GH-releasing peptide-6 (GHRP-6) and GHRP-2 on GH release was investigated in ovine and rat pituitary cells in vitro.
Report a problemFND-0869913304
04LabIn vitro (cells)Mechanism
In ovine somatotrophs, the adenylyl cyclase inhibitor MDL 12,330A partially reduced the GH release caused by GHRP‑6.
In plain wordsIn lab tests on sheep pituitary cells, an enzyme blocker partly reduced growth hormone release caused by GHRP‑6.
Peptide
GHRP-6
Outcome
DecreaseGH release with adenylyl cyclase inhibitor MDL 12,330A
Study
sheepn: not reported in source
partially purified ovine somatotrophs
Outcome group
Receptor activity and mechanism · Endocrine and hormones
Studied dose
Dose not stated
Source sentences
Summary
MDL 12,330A partially reduced the effect.
1 context sentence(design, population, supporting numbers)
Summary · context
The mechanism of action of GH-releasing peptide-6 (GHRP-6) and GHRP-2 on GH release was investigated in ovine and rat pituitary cells in vitro.
Report a problemFND-0869913305
05LabIn vitro (cells)Mechanism
In ovine somatotrophs, a GRF receptor antagonist did not affect GH release induced by GHRP‑6.
In plain wordsIn lab tests on sheep pituitary cells, blocking the GRF receptor did not change growth hormone release caused by GHRP‑6.
Peptide
GHRP-6
Outcome
No significant changeGH release with GRF receptor antagonist
Study
sheepn: not reported in source
partially purified ovine somatotrophs
Outcome group
Receptor activity and mechanism · Endocrine and hormones
Studied dose
Dose not stated
Source sentences
Summary
An antagonist for the GRF receptor, [Ac-Tyr1,D-Arg2]-GRF 1-29, significantly diminished the effect of GHRP-2 and GRF on cAMP accumulation and GH release, but did not affect GH release induced by GHRP-6.
1 context sentence(design, population, supporting numbers)
Summary · context
The mechanism of action of GH-releasing peptide-6 (GHRP-6) and GHRP-2 on GH release was investigated in ovine and rat pituitary cells in vitro.
Report a problemFND-0869913306
06LabIn vitro (cells)Mechanism
In ovine somatotrophs, somatostatin prevented the GH release response to GHRP‑6.
In plain wordsIn lab tests on sheep pituitary cells, somatostatin stopped growth hormone release caused by GHRP‑6.
Peptide
GHRP-6
Outcome
DecreaseGH release with somatostatin
Study
sheepn: not reported in source
partially purified ovine somatotrophs
Outcome group
Receptor activity and mechanism · Endocrine and hormones
Studied dose
Dose not stated
Source sentences
Summary
Somatostatin prevented cAMP accumulation and GH release responses to GHRP-2, GRF and GHRP-6.
1 context sentence(design, population, supporting numbers)
Summary · context
The mechanism of action of GH-releasing peptide-6 (GHRP-6) and GHRP-2 on GH release was investigated in ovine and rat pituitary cells in vitro.
Report a problemFND-0869913307
07LabIn vitro (cells)Mechanism
In ovine somatotrophs, Ca2+ channel blockade totally prevented GH release in response to GHRP‑6.
In plain wordsIn lab tests on sheep pituitary cells, blocking calcium channels fully stopped growth hormone release caused by GHRP‑6.
Peptide
GHRP-6
Outcome
DecreaseGH release with Ca2+ channel blockade
Study
sheepn: not reported in source
partially purified ovine somatotrophs
Outcome group
Receptor activity and mechanism · Endocrine and hormones
Studied dose
Dose not stated
Source sentences
Summary
but totally prevented GH release in response to GHRP-2, GRF and GHRP-6.
1 context sentence(design, population, supporting numbers)
Summary · context
The mechanism of action of GH-releasing peptide-6 (GHRP-6) and GHRP-2 on GH release was investigated in ovine and rat pituitary cells in vitro.
Report a problemFND-0869913308
08LabIn vitro (cells)Mechanism
In rat pituitary cell cultures, GHRP‑6 alone had no effect on cAMP levels.
In plain wordsIn lab tests on rat pituitary cells, GHRP‑6 alone did not change cAMP, a cell signal.
Peptide
GHRP-6
Outcome
No significant changecAMP level in rat pituitary cells
Study
ratn: not reported in source
rat pituitary cell cultures
Outcome group
Receptor activity and mechanism · Endocrine and hormones
Studied dose
Dose not stated
Source sentences
Summary
GHRP-6 had no effect on cAMP levels
1 context sentence(design, population, supporting numbers)
Summary · context
The mechanism of action of GH-releasing peptide-6 (GHRP-6) and GHRP-2 on GH release was investigated in ovine and rat pituitary cells in vitro.
Report a problemFND-0869913309
09LabIn vitro (cells)Mechanism
In rat pituitary cell cultures, GHRP‑6 potentiated the effect of GRF on cAMP accumulation compared with GRF alone.
In plain wordsIn lab tests on rat pituitary cells, GHRP‑6 boosted the cAMP rise caused by GRF.
Peptide
GHRP-6
Outcome
IncreaseGRF-induced cAMP accumulation in rat cells
Study
ratn: not reported in source
rat pituitary cell cultures
Comparator
GRF alone
Outcome group
Receptor activity and mechanism · Endocrine and hormones
Studied dose
Dose not stated
Source sentences
Summary
but potentiated the effect of GRF on cAMP accumulation.
1 context sentence(design, population, supporting numbers)
Summary · context
The mechanism of action of GH-releasing peptide-6 (GHRP-6) and GHRP-2 on GH release was investigated in ovine and rat pituitary cells in vitro.
Report a problemFND-0869913310
10LabIn vitro (cells)Mechanism
In sheep somatotrophs, the synergistic effect of GRF and GHRP‑6 on cAMP accumulation did not occur.
In plain wordsIn lab tests on sheep pituitary cells, GHRP‑6 did not boost the cAMP rise caused by GRF, unlike in rat cells.
Peptide
GHRP-6
Outcome
No significant changeGRF plus GHRP-6 synergy on cAMP in sheep cells
Study
sheepn: not reported in source
partially purified ovine somatotrophs
Comparator
GRF alone
Outcome group
Receptor activity and mechanism · Endocrine and hormones
Studied dose
Dose not stated
Source sentences
Summary
The synergistic effect of GRF and GHRP-6 on cAMP accumulation did not occur in sheep somatotrophs.
1 context sentence(design, population, supporting numbers)
Summary · context
The mechanism of action of GH-releasing peptide-6 (GHRP-6) and GHRP-2 on GH release was investigated in ovine and rat pituitary cells in vitro.
Report a problemFND-0869913311
11LabIn vitro (cells)Activity measurement
In partially purified sheep somatotrophs, GHRP‑2 increased intracellular cAMP concentrations in a dose-dependent manner.
In plain wordsIn lab tests with sheep pituitary cells, GHRP‑2 raised cAMP, a cell signal, as the dose went up.
Peptide
GHRP-2
Outcome
IncreaseIntracellular cAMP in sheep somatotrophs
Study
sheepn: not reported in source
partially purified ovine pituitary somatotrophs
Outcome group
Growth hormone and IGF-1 · Endocrine and hormones
Studied dose
Dose not stated
Source sentences
Summary
In partially purified sheep somatotrophs, GHRP-2 and GH-releasing factor (GRF) increased intracellular cyclic AMP (cAMP) concentrations and caused GH release in a dose-dependent manner
1 context sentence(design, population, supporting numbers)
Summary · context
The mechanism of action of GH-releasing peptide-6 (GHRP-6) and GHRP-2 on GH release was investigated in ovine and rat pituitary cells in vitro.
Report a problemFND-0869913312
12LabIn vitro (cells)Activity measurement
In partially purified sheep somatotrophs, GHRP‑2 caused GH release in a dose-dependent manner.
In plain wordsIn lab tests with sheep pituitary cells, GHRP‑2 made the cells release growth hormone as the dose went up.
Peptide
GHRP-2
Outcome
IncreaseGH release from sheep somatotrophs
Study
sheepn: not reported in source
partially purified ovine pituitary somatotrophs
Outcome group
Growth hormone and IGF-1 · Endocrine and hormones
Studied dose
Dose not stated
Source sentences
Summary
In partially purified sheep somatotrophs, GHRP-2 and GH-releasing factor (GRF) increased intracellular cyclic AMP (cAMP) concentrations and caused GH release in a dose-dependent manner
1 context sentence(design, population, supporting numbers)
Summary · context
The mechanism of action of GH-releasing peptide-6 (GHRP-6) and GHRP-2 on GH release was investigated in ovine and rat pituitary cells in vitro.
Report a problemFND-0869913313
13LabIn vitro (cells)Activity measurement
In rat pituitary cells, GHRP‑2 did not cause cAMP accumulation, unlike in sheep somatotrophs.
In plain wordsIn lab tests with rat pituitary cells, GHRP‑2 did not raise cAMP, although it did in sheep cells.
Peptide
GHRP-2
Outcome
No significant changecAMP accumulation in rat pituitary cells
Study
ratn: not reported in source
rat pituitary cell cultures
Outcome group
Growth hormone and IGF-1 · Endocrine and hormones
Studied dose
Dose not stated
Source sentences
Summary
Whereas GHRP-2 caused cAMP accumulation in sheep somatotrophs, it did not do so in rat pituitary cells.
1 context sentence(design, population, supporting numbers)
Summary · context
The mechanism of action of GH-releasing peptide-6 (GHRP-6) and GHRP-2 on GH release was investigated in ovine and rat pituitary cells in vitro.
Report a problemFND-0869913326

Studies that did not test the peptide alone13

In this study, the peptide was a lab tool or a carrier, part of a mix, or the subject of a lab method. These results are not evidence about taking the peptide.

14LabNot a test of GHRP-6 aloneIn vitro (cells)Activity measurement
In ovine somatotrophs, combined maximal doses of GHRP‑6 and GHRP-2 produced an additive effect on GH release only, not on cAMP.
In plain wordsIn lab tests on sheep pituitary cells, GHRP‑6 and GHRP-2 together had an additive effect on growth hormone release.
Peptide
GHRP-6
Outcome
IncreaseGH release with GHRP-6 plus GHRP-2
Study
sheepn: not reported in source
partially purified ovine somatotrophs
Outcome group
Receptor activity and mechanism · Endocrine and hormones
Source sentences
Summary
combined GHRP-6 and GHRP-2 at maximal doses produced an additive effect on GH release only.
1 context sentence(design, population, supporting numbers)
Summary · context
The mechanism of action of GH-releasing peptide-6 (GHRP-6) and GHRP-2 on GH release was investigated in ovine and rat pituitary cells in vitro.
In this study, GHRP-6 was a lab tool or a carrier, part of a mix, or the subject of a lab method. This result is not evidence about taking GHRP-6.
Report a problemFND-0869913303
15LabNot a test of GHRP-2 aloneIn vitro (cells)Activity measurement
In sheep somatotrophs, maximal doses of GRF and GHRP‑2 together had an additive effect on cAMP levels.
In plain wordsIn lab tests with sheep pituitary cells, GRF and GHRP‑2 given together raised cAMP more than either alone.
Peptide
GHRP-2
Outcome
IncreasecAMP with GRF plus GHRP-2 at maximal doses
Study
sheepn: not reported in source
partially purified ovine pituitary somatotrophs
Outcome group
Growth hormone and IGF-1 · Endocrine and hormones
Source sentences
Summary
An additive effect of maximal doses of GRF and GHRP-2 was observed in both cAMP and GH levels
1 context sentence(design, population, supporting numbers)
Summary · context
The mechanism of action of GH-releasing peptide-6 (GHRP-6) and GHRP-2 on GH release was investigated in ovine and rat pituitary cells in vitro.
In this study, GHRP-2 was a lab tool or a carrier, part of a mix, or the subject of a lab method. This result is not evidence about taking GHRP-2.
Report a problemFND-0869913314
16LabNot a test of GHRP-2 aloneIn vitro (cells)Activity measurement
In sheep somatotrophs, maximal doses of GRF and GHRP‑2 together had an additive effect on GH levels.
In plain wordsIn lab tests with sheep pituitary cells, GRF and GHRP‑2 given together released more growth hormone than either alone.
Peptide
GHRP-2
Outcome
IncreaseGH release with GRF plus GHRP-2 at maximal doses
Study
sheepn: not reported in source
partially purified ovine pituitary somatotrophs
Outcome group
Growth hormone and IGF-1 · Endocrine and hormones
Source sentences
Summary
An additive effect of maximal doses of GRF and GHRP-2 was observed in both cAMP and GH levels
1 context sentence(design, population, supporting numbers)
Summary · context
The mechanism of action of GH-releasing peptide-6 (GHRP-6) and GHRP-2 on GH release was investigated in ovine and rat pituitary cells in vitro.
In this study, GHRP-2 was a lab tool or a carrier, part of a mix, or the subject of a lab method. This result is not evidence about taking GHRP-2.
Report a problemFND-0869913315
17LabNot a test of GHRP-2 aloneIn vitro (cells)Activity measurement
In sheep somatotrophs, combined GHRP-6 and GHRP‑2 at maximal doses produced an additive effect on GH release only, not on cAMP.
In plain wordsIn lab tests with sheep pituitary cells, GHRP-6 and GHRP‑2 together released more growth hormone than either alone, but cAMP did not add up.
Peptide
GHRP-2
Outcome
IncreaseGH release with GHRP-6 plus GHRP-2 at maximal doses
Study
sheepn: not reported in source
partially purified ovine pituitary somatotrophs
Outcome group
Growth hormone and IGF-1 · Endocrine and hormones
Source sentences
Summary
combined GHRP-6 and GHRP-2 at maximal doses produced an additive effect on GH release only.
1 context sentence(design, population, supporting numbers)
Summary · context
The mechanism of action of GH-releasing peptide-6 (GHRP-6) and GHRP-2 on GH release was investigated in ovine and rat pituitary cells in vitro.
In this study, GHRP-2 was a lab tool or a carrier, part of a mix, or the subject of a lab method. This result is not evidence about taking GHRP-2.
Report a problemFND-0869913316
18LabNot a test of GHRP-2 aloneIn vitro (cells)Mechanism
In sheep somatotrophs, the adenylyl cyclase inhibitor MDL 12,330A prevented the cAMP accumulation caused by GHRP‑2 or GRF.
In plain wordsIn lab tests with sheep pituitary cells, a drug that blocks cAMP production stopped the cAMP rise caused by GHRP‑2.
Peptide
GHRP-2
Outcome
DecreasecAMP accumulation after adenylyl cyclase inhibitor MDL 12,330A
Study
sheepn: not reported in source
partially purified ovine pituitary somatotrophs
Outcome group
Growth hormone and IGF-1 · Endocrine and hormones
Source sentences
Summary
Pretreatment of the cells with MDL 12,330A, an adenylyl cyclase inhibitor, prevented cAMP accumulation and the subsequent release of GH that was caused by either GHRP-2 or GRF.
1 context sentence(design, population, supporting numbers)
Summary · context
The mechanism of action of GH-releasing peptide-6 (GHRP-6) and GHRP-2 on GH release was investigated in ovine and rat pituitary cells in vitro.
In this study, GHRP-2 was a lab tool or a carrier, part of a mix, or the subject of a lab method. This result is not evidence about taking GHRP-2.
Report a problemFND-0869913317
19LabNot a test of GHRP-2 aloneIn vitro (cells)Mechanism
In sheep somatotrophs, the adenylyl cyclase inhibitor MDL 12,330A prevented the GH release caused by GHRP‑2 or GRF.
In plain wordsIn lab tests with sheep pituitary cells, a drug that blocks cAMP production stopped GHRP‑2 from releasing growth hormone.
Peptide
GHRP-2
Outcome
DecreaseGH release after adenylyl cyclase inhibitor MDL 12,330A
Study
sheepn: not reported in source
partially purified ovine pituitary somatotrophs
Outcome group
Growth hormone and IGF-1 · Endocrine and hormones
Source sentences
Summary
Pretreatment of the cells with MDL 12,330A, an adenylyl cyclase inhibitor, prevented cAMP accumulation and the subsequent release of GH that was caused by either GHRP-2 or GRF.
1 context sentence(design, population, supporting numbers)
Summary · context
The mechanism of action of GH-releasing peptide-6 (GHRP-6) and GHRP-2 on GH release was investigated in ovine and rat pituitary cells in vitro.
In this study, GHRP-2 was a lab tool or a carrier, part of a mix, or the subject of a lab method. This result is not evidence about taking GHRP-2.
Report a problemFND-0869913318
20LabNot a test of GHRP-2 aloneIn vitro (cells)Mechanism
In sheep somatotrophs, the cAMP antagonist Rp-cAMP blocked GH release in response to GHRP‑2 and GRF.
In plain wordsIn lab tests with sheep pituitary cells, a cAMP blocker stopped GHRP‑2 from releasing growth hormone.
Peptide
GHRP-2
Outcome
DecreaseGH release after cAMP antagonist Rp-cAMP
Study
sheepn: not reported in source
partially purified ovine pituitary somatotrophs
Outcome group
Growth hormone and IGF-1 · Endocrine and hormones
Source sentences
Summary
The cAMP antagonist, Rp-cAMP also blocked GH release in response to GHRP-2 and GRF.
1 context sentence(design, population, supporting numbers)
Summary · context
The mechanism of action of GH-releasing peptide-6 (GHRP-6) and GHRP-2 on GH release was investigated in ovine and rat pituitary cells in vitro.
In this study, GHRP-2 was a lab tool or a carrier, part of a mix, or the subject of a lab method. This result is not evidence about taking GHRP-2.
Report a problemFND-0869913319
21LabNot a test of GHRP-2 aloneIn vitro (cells)Mechanism
In sheep somatotrophs, the GRF receptor antagonist [Ac-Tyr1,D-Arg2]-GRF 1-29 significantly diminished the cAMP accumulation caused by GHRP‑2 and GRF.
In plain wordsIn lab tests with sheep pituitary cells, a GRF receptor blocker reduced the cAMP rise caused by GHRP‑2.
Peptide
GHRP-2
Outcome
DecreasecAMP accumulation after GRF receptor antagonist
Study
sheepn: not reported in source
partially purified ovine pituitary somatotrophs
Outcome group
Growth hormone and IGF-1 · Endocrine and hormones
Source sentences
Summary
An antagonist for the GRF receptor, [Ac-Tyr1,D-Arg2]-GRF 1-29, significantly diminished the effect of GHRP-2 and GRF on cAMP accumulation and GH release, but did not affect GH release induced by GHRP-6.
1 context sentence(design, population, supporting numbers)
Summary · context
The mechanism of action of GH-releasing peptide-6 (GHRP-6) and GHRP-2 on GH release was investigated in ovine and rat pituitary cells in vitro.
In this study, GHRP-2 was a lab tool or a carrier, part of a mix, or the subject of a lab method. This result is not evidence about taking GHRP-2.
Report a problemFND-0869913320
22LabNot a test of GHRP-2 aloneIn vitro (cells)Mechanism
In sheep somatotrophs, the GRF receptor antagonist [Ac-Tyr1,D-Arg2]-GRF 1-29 significantly diminished the GH release caused by GHRP‑2 and GRF.
In plain wordsIn lab tests with sheep pituitary cells, a GRF receptor blocker reduced growth hormone release caused by GHRP‑2.
Peptide
GHRP-2
Outcome
DecreaseGH release after GRF receptor antagonist
Study
sheepn: not reported in source
partially purified ovine pituitary somatotrophs
Outcome group
Growth hormone and IGF-1 · Endocrine and hormones
Source sentences
Summary
An antagonist for the GRF receptor, [Ac-Tyr1,D-Arg2]-GRF 1-29, significantly diminished the effect of GHRP-2 and GRF on cAMP accumulation and GH release, but did not affect GH release induced by GHRP-6.
1 context sentence(design, population, supporting numbers)
Summary · context
The mechanism of action of GH-releasing peptide-6 (GHRP-6) and GHRP-2 on GH release was investigated in ovine and rat pituitary cells in vitro.
In this study, GHRP-2 was a lab tool or a carrier, part of a mix, or the subject of a lab method. This result is not evidence about taking GHRP-2.
Report a problemFND-0869913321
23LabNot a test of GHRP-2 aloneIn vitro (cells)Mechanism
In sheep somatotrophs, somatostatin prevented the cAMP accumulation caused by GHRP‑2, GRF and GHRP-6.
In plain wordsIn lab tests with sheep pituitary cells, somatostatin stopped the cAMP rise caused by GHRP‑2.
Peptide
GHRP-2
Outcome
DecreasecAMP accumulation after somatostatin
Study
sheepn: not reported in source
partially purified ovine pituitary somatotrophs
Outcome group
Growth hormone and IGF-1 · Endocrine and hormones
Source sentences
Summary
Somatostatin prevented cAMP accumulation and GH release responses to GHRP-2, GRF and GHRP-6.
1 context sentence(design, population, supporting numbers)
Summary · context
The mechanism of action of GH-releasing peptide-6 (GHRP-6) and GHRP-2 on GH release was investigated in ovine and rat pituitary cells in vitro.
In this study, GHRP-2 was a lab tool or a carrier, part of a mix, or the subject of a lab method. This result is not evidence about taking GHRP-2.
Report a problemFND-0869913322
24LabNot a test of GHRP-2 aloneIn vitro (cells)Mechanism
In sheep somatotrophs, somatostatin prevented the GH release caused by GHRP‑2, GRF and GHRP-6.
In plain wordsIn lab tests with sheep pituitary cells, somatostatin stopped GHRP‑2 from releasing growth hormone.
Peptide
GHRP-2
Outcome
DecreaseGH release after somatostatin
Study
sheepn: not reported in source
partially purified ovine pituitary somatotrophs
Outcome group
Growth hormone and IGF-1 · Endocrine and hormones
Source sentences
Summary
Somatostatin prevented cAMP accumulation and GH release responses to GHRP-2, GRF and GHRP-6.
1 context sentence(design, population, supporting numbers)
Summary · context
The mechanism of action of GH-releasing peptide-6 (GHRP-6) and GHRP-2 on GH release was investigated in ovine and rat pituitary cells in vitro.
In this study, GHRP-2 was a lab tool or a carrier, part of a mix, or the subject of a lab method. This result is not evidence about taking GHRP-2.
Report a problemFND-0869913323
25LabNot a test of GHRP-2 aloneIn vitro (cells)Mechanism
In sheep somatotrophs, Ca2+ channel blockade did not affect the cAMP increase caused by GHRP‑2 or GRF.
In plain wordsIn lab tests with sheep pituitary cells, blocking calcium channels did not change the cAMP rise caused by GHRP‑2.
Peptide
GHRP-2
Outcome
No significant changecAMP increase after Ca2+ channel blockade
Study
sheepn: not reported in source
partially purified ovine pituitary somatotrophs
Outcome group
Growth hormone and IGF-1 · Endocrine and hormones
Source sentences
Summary
Ca2+ channel blockade did not affect the cAMP increase in response to GHRP-2 or GRF but totally prevented GH release in response to GHRP-2, GRF and GHRP-6.
1 context sentence(design, population, supporting numbers)
Summary · context
The mechanism of action of GH-releasing peptide-6 (GHRP-6) and GHRP-2 on GH release was investigated in ovine and rat pituitary cells in vitro.
In this study, GHRP-2 was a lab tool or a carrier, part of a mix, or the subject of a lab method. This result is not evidence about taking GHRP-2.
Report a problemFND-0869913324
26LabNot a test of GHRP-2 aloneIn vitro (cells)Mechanism
In sheep somatotrophs, Ca2+ channel blockade totally prevented the GH release caused by GHRP‑2, GRF and GHRP-6.
In plain wordsIn lab tests with sheep pituitary cells, blocking calcium channels fully stopped GHRP‑2 from releasing growth hormone.
Peptide
GHRP-2
Outcome
DecreaseGH release after Ca2+ channel blockade
Study
sheepn: not reported in source
partially purified ovine pituitary somatotrophs
Outcome group
Growth hormone and IGF-1 · Endocrine and hormones
Source sentences
Summary
Ca2+ channel blockade did not affect the cAMP increase in response to GHRP-2 or GRF but totally prevented GH release in response to GHRP-2, GRF and GHRP-6.
1 context sentence(design, population, supporting numbers)
Summary · context
The mechanism of action of GH-releasing peptide-6 (GHRP-6) and GHRP-2 on GH release was investigated in ovine and rat pituitary cells in vitro.
In this study, GHRP-2 was a lab tool or a carrier, part of a mix, or the subject of a lab method. This result is not evidence about taking GHRP-2.
Report a problemFND-0869913325

AI wrote and checked these results. AI can make mistakes, so check important results against the source.

Version history

PepBank keeps every version of the data on this study. Status changes from Retraction Watch, Europe PMC and Crossref are listed with their source.

Versions 4 to 18Current
28 Oct 2026, 02:02 UTC
The same notice was added 15 times, first on 13 Oct 2026, 21:44 UTC. The status did not change between them.
Study results updated
Version 3
13 Oct 2026, 16:31 UTC
Study data updated · source update from Europe PMC
Version 2
12 Oct 2026, 12:58 UTC
Study results updated
Version 1
9 Oct 2026, 03:13 UTC
First added · source update from Europe PMC

Subjects

Terms as the source lists them.

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