Effects of enclomiphene and zuclomiphene on basal and gonadotrophin-stimulated progesterone secretion by isolated subpopulations of small and large ovine luteal cells.

Opsahl MS, Fitz TA, Rexroad CE Jr, Fritz MA

Human reproduction (Oxford, England)1 Jun 1996Cited by 7 (Europe PMC)

PMID8671435DOI10.1093/oxfordjournals.humrep.a019367PepBankPUB-08671435

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Summary

The text of this summary is not shown here, because the article license does not allow reuse. Read it at the source (opens in a new tab)

Text rights: Publisher copyright. Results still quote short attributed sentences.

Results from this study6

4 results from studies that gave the peptide. No result from this study comes from people.

01LabIn vitro (cells)Efficacy outcome
In ovine small luteal cells, enclomiphene dose-dependently inhibited basal progesterone secretion under all substrate conditions.
In plain wordsIn sheep cells, enclomiphene reduced basal progesterone secretion from small luteal cells as the dose increased.
Peptide
Enclomiphene
Outcome
DecreaseBasal progesterone secretion in small luteal cells
Study
ovinen: not reported in source
isolated small and large luteal cells
Outcome group
Sex hormones and fertility · Reproductive and sexual health
Studied dose
3-100 microM · 48-120 hAs given in this study (ovine).
Source sentences
Summary
Enclomiphene, zuclomiphene, and oestradiol exhibited equivalent dose-dependent inhibitory effects on basal and gonadotrophin-stimulated small and large ovine luteal cell progesterone secretion under all substrate conditions.
2 context sentences(design, population, supporting numbers)
Summary · context
Isolated large and small luteal cells derived from intact, enucleated ovine corpora lutea were incubated for 48-120 h with or without 22R-hydroxycholesterol or pregnenolone (2.5 microM) and a range of enclomiphene, zuclomiphene, and/or oestradiol concentrations (3-100 microM), both with and without…
Summary · context
We examined the effects of enclomiphene and zuclomiphene, alone and in combination with oestradiol, on basal and gonadotrophin-stimulated progesterone secretion by isolated subpopulations of both large (granulosa-lutein) and small (theca-lutein) ovine luteal cells.
Report a problemFND-0867143501
02LabIn vitro (cells)Efficacy outcome
In ovine small luteal cells, enclomiphene dose-dependently inhibited gonadotrophin-stimulated progesterone secretion under all substrate conditions.
In plain wordsIn sheep cells, enclomiphene reduced stimulated progesterone secretion from small luteal cells as the dose increased.
Peptide
Enclomiphene
Outcome
DecreaseStimulated progesterone secretion in small luteal cells
Study
ovinen: not reported in source
isolated small and large luteal cells
Outcome group
Sex hormones and fertility · Reproductive and sexual health
Studied dose
3-100 microM · 48-120 hAs given in this study (ovine).
Source sentences
Summary
Enclomiphene, zuclomiphene, and oestradiol exhibited equivalent dose-dependent inhibitory effects on basal and gonadotrophin-stimulated small and large ovine luteal cell progesterone secretion under all substrate conditions.
2 context sentences(design, population, supporting numbers)
Summary · context
Isolated large and small luteal cells derived from intact, enucleated ovine corpora lutea were incubated for 48-120 h with or without 22R-hydroxycholesterol or pregnenolone (2.5 microM) and a range of enclomiphene, zuclomiphene, and/or oestradiol concentrations (3-100 microM), both with and without…
Summary · context
We examined the effects of enclomiphene and zuclomiphene, alone and in combination with oestradiol, on basal and gonadotrophin-stimulated progesterone secretion by isolated subpopulations of both large (granulosa-lutein) and small (theca-lutein) ovine luteal cells.
Report a problemFND-0867143502
03LabIn vitro (cells)Efficacy outcome
In ovine large luteal cells, enclomiphene dose-dependently inhibited basal progesterone secretion under all substrate conditions.
In plain wordsIn sheep cells, enclomiphene reduced basal progesterone secretion from large luteal cells as the dose increased.
Peptide
Enclomiphene
Outcome
DecreaseBasal progesterone secretion in large luteal cells
Study
ovinen: not reported in source
isolated small and large luteal cells
Outcome group
Sex hormones and fertility · Reproductive and sexual health
Studied dose
3-100 microM · 48-120 hAs given in this study (ovine).
Source sentences
Summary
Enclomiphene, zuclomiphene, and oestradiol exhibited equivalent dose-dependent inhibitory effects on basal and gonadotrophin-stimulated small and large ovine luteal cell progesterone secretion under all substrate conditions.
2 context sentences(design, population, supporting numbers)
Summary · context
Isolated large and small luteal cells derived from intact, enucleated ovine corpora lutea were incubated for 48-120 h with or without 22R-hydroxycholesterol or pregnenolone (2.5 microM) and a range of enclomiphene, zuclomiphene, and/or oestradiol concentrations (3-100 microM), both with and without…
Summary · context
We examined the effects of enclomiphene and zuclomiphene, alone and in combination with oestradiol, on basal and gonadotrophin-stimulated progesterone secretion by isolated subpopulations of both large (granulosa-lutein) and small (theca-lutein) ovine luteal cells.
Report a problemFND-0867143503
04LabIn vitro (cells)Efficacy outcome
In ovine large luteal cells, enclomiphene dose-dependently inhibited gonadotrophin-stimulated progesterone secretion under all substrate conditions.
In plain wordsIn sheep cells, enclomiphene reduced stimulated progesterone secretion from large luteal cells as the dose increased.
Peptide
Enclomiphene
Outcome
DecreaseStimulated progesterone secretion in large luteal cells
Study
ovinen: not reported in source
isolated small and large luteal cells
Outcome group
Sex hormones and fertility · Reproductive and sexual health
Studied dose
3-100 microM · 48-120 hAs given in this study (ovine).
Source sentences
Summary
Enclomiphene, zuclomiphene, and oestradiol exhibited equivalent dose-dependent inhibitory effects on basal and gonadotrophin-stimulated small and large ovine luteal cell progesterone secretion under all substrate conditions.
2 context sentences(design, population, supporting numbers)
Summary · context
Isolated large and small luteal cells derived from intact, enucleated ovine corpora lutea were incubated for 48-120 h with or without 22R-hydroxycholesterol or pregnenolone (2.5 microM) and a range of enclomiphene, zuclomiphene, and/or oestradiol concentrations (3-100 microM), both with and without…
Summary · context
We examined the effects of enclomiphene and zuclomiphene, alone and in combination with oestradiol, on basal and gonadotrophin-stimulated progesterone secretion by isolated subpopulations of both large (granulosa-lutein) and small (theca-lutein) ovine luteal cells.
Report a problemFND-0867143504

Studies that did not test the peptide alone2

In this study, Enclomiphene was a lab tool or a carrier, part of a mix, or the subject of a lab method. These results are not evidence about taking Enclomiphene.

05LabNot a test of Enclomiphene aloneIn vitro (cells)Activity measurement
In ovine small and large luteal cells, sensitivity to clomiphene inhibition, covering the tested enclomiphene and zuclomiphene isomers, increased with culture time.
In plain wordsIn sheep cells, sensitivity to inhibition by clomiphene isomers, including enclomiphene, increased with culture time.
Peptide
Enclomiphene
Outcome
IncreaseTime-dependent sensitivity to inhibition
Study
ovinen: not reported in source
isolated small and large luteal cells
Outcome group
Sex hormones and fertility · Reproductive and sexual health
Source sentences
Summary
Both cell types became more sensitive to clomiphene inhibition with increasing time in culture.
1 context sentence(design, population, supporting numbers)
Summary · context
We examined the effects of enclomiphene and zuclomiphene, alone and in combination with oestradiol, on basal and gonadotrophin-stimulated progesterone secretion by isolated subpopulations of both large (granulosa-lutein) and small (theca-lutein) ovine luteal cells.
In this study, Enclomiphene was a lab tool or a carrier, part of a mix, or the subject of a lab method. This result is not evidence about taking Enclomiphene.
Report a problemFND-0867143505
06LabNot a test of Enclomiphene aloneIn vitro (cells)Activity measurement
In ovine luteal cells, oestradiol with enclomiphene additively inhibited progesterone secretion in longer-term cultures and never showed antagonism.
In plain wordsIn sheep cells, oestradiol with enclomiphene additively inhibited progesterone secretion in longer cultures and never showed opposing effects.
Peptide
Enclomiphene
Outcome
IncreaseAdditive inhibition of progesterone secretion with oestradiol
Study
ovinen: not reported in source
isolated small and large luteal cells
Outcome group
Sex hormones and fertility · Reproductive and sexual health
Source sentences
Summary
In combined treatments, the effects of oestradiol and either enclomiphene or zuclomiphene became additive in longer-term cultures and were never antagonistic.
1 context sentence(design, population, supporting numbers)
Summary · context
We examined the effects of enclomiphene and zuclomiphene, alone and in combination with oestradiol, on basal and gonadotrophin-stimulated progesterone secretion by isolated subpopulations of both large (granulosa-lutein) and small (theca-lutein) ovine luteal cells.
In this study, Enclomiphene was a lab tool or a carrier, part of a mix, or the subject of a lab method. This result is not evidence about taking Enclomiphene.
Report a problemFND-0867143506

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PepBank keeps every version of the data on this study. Status changes from Retraction Watch, Europe PMC and Crossref are listed with their source.

Version 2Current
21 Oct 2026, 15:58 UTC
Study results updated
Version 1
21 Oct 2026, 12:50 UTC
First added · source update from Europe PMC

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