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Study · In vitro (cells) · rat · 1995

5 results

Glycyl-histidyl-lysine interacts with the angiotensin II AT1 receptor.

García-Sáinz JA, Olivares-Reyes JA

Peptides1 Jan 1995Cited by 5 (Europe PMC)

PMID8545239 (opens in a new tab)DOI10.1016/0196-9781(95)02015-o (opens in a new tab)PepBankPUB-08545239
Publisher pagePubMedAsk about this study
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Summary

The text of this summary is not shown here, because the article license does not allow reuse. Read it at the source (opens in a new tab)

Text rights: Publisher copyright. Results still quote short attributed sentences.

Results from this study5

4 results from studies that gave the peptide. No result from this study comes from people.

  1. 01LabIn vitro (cells)Mechanism

    In isolated rat hepatocytes, Gly-His-Lys dose-dependently stimulated phosphorylase a activity.

    In plain wordsIn cells from rat liver, Gly-His-Lys increased phosphorylase a activity in a dose-dependent manner.

    Peptide
    GHK (glycyl-L-histidyl-L-lysine)
    Outcome
    IncreasePhosphorylase a activity
    Study

    ratn: not reported in source

    isolated rat hepatocytes
    Outcome group
    Receptor activity and mechanism · Liver
    Studied dose
    Dose not stated

    Source sentences

    Summary
    Gly-His-Lys, a tripeptide isolated from human plasma that increases the growth rate of many cells, stimulated in dose-dependent fashion the activity of phosphorylase a in isolated rat hepatocytes.
  2. 02LabIn vitro (cells)Mechanism

    In isolated rat hepatocytes, Gly-His-Lys stimulation was associated with increased IP3 production.

    In plain wordsIn cells from rat liver, Gly-His-Lys stimulation was associated with increased IP3 production.

    Peptide
    GHK (glycyl-L-histidyl-L-lysine)
    Outcome
    IncreaseIP3 production
    Study

    ratn: not reported in source

    isolated rat hepatocytes
    Outcome group
    Receptor activity and mechanism · Liver
    Studied dose
    Dose not stated

    Source sentences

    Summary
    Such effect was associated to increases in both IP3 production and [Ca++]i.
    1 context sentence
    Summary · context
    Gly-His-Lys, a tripeptide isolated from human plasma that increases the growth rate of many cells, stimulated in dose-dependent fashion the activity of phosphorylase a in isolated rat hepatocytes.
  3. 03LabIn vitro (cells)Mechanism

    In isolated rat hepatocytes, Gly-His-Lys stimulation was associated with increased [Ca++]i.

    In plain wordsIn cells from rat liver, Gly-His-Lys stimulation was associated with increased intracellular calcium.

    Peptide
    GHK (glycyl-L-histidyl-L-lysine)
    Outcome
    IncreaseIntracellular calcium
    Study

    ratn: not reported in source

    isolated rat hepatocytes
    Outcome group
    Receptor activity and mechanism · Liver
    Studied dose
    Dose not stated

    Source sentences

    Summary
    Such effect was associated to increases in both IP3 production and [Ca++]i.
    1 context sentence
    Summary · context
    Gly-His-Lys, a tripeptide isolated from human plasma that increases the growth rate of many cells, stimulated in dose-dependent fashion the activity of phosphorylase a in isolated rat hepatocytes.
  4. 04LabIn vitro (cell-free)Mechanism

    In radioligand competition experiments, Gly-His-Lys interacted with AT1 receptors.

    In plain wordsIn lab tests, Gly-His-Lys interacted with AT1 receptors.

    Peptide
    GHK (glycyl-L-histidyl-L-lysine)
    Outcome
    Not applicableAT1 receptor interaction
    Study

    n: not reported in source

    radioligand binding competition assay
    Outcome group
    Receptor activity and mechanism · Pharmacology
    Studied dose
    Dose not stated

    Source sentences

    Summary
    Binding competition experiments using the radioligand [125I][Sar1-Ile8]angiotensin II clearly indicated that Gly-His-Lys interacts with AT1 receptors.

Studies that did not test the peptide alone1

In this study, GHK (glycyl-L-histidyl-L-lysine) was a lab tool or a carrier, part of a mix, or the subject of a lab method. These results are not evidence about taking GHK (glycyl-L-histidyl-L-lysine).

  1. 05LabNot a test of GHK (glycyl-L-histidyl-L-lysine) aloneIn vitro (cells)Mechanism

    In isolated rat hepatocytes, losartan antagonized Gly-His-Lys effects, suggesting AT1 receptor involvement.

    In plain wordsIn cells from rat liver, losartan opposed Gly-His-Lys effects, suggesting AT1 receptor involvement.

    Peptide
    GHK (glycyl-L-histidyl-L-lysine)
    Outcome
    DecreaseLosartan antagonism
    Study

    ratn: not reported in source

    isolated rat hepatocytes
    Outcome group
    Receptor activity and mechanism · Liver

    Source sentences

    Summary
    Interestingly, these effects of Gly-His-Lys were antagonized by losartan, a nonpeptide angiotensin II receptor antagonist (AT1 selective), which suggested that these receptors were involved in its effect.

    In this study, GHK (glycyl-L-histidyl-L-lysine) was a lab tool or a carrier, part of a mix, or the subject of a lab method. This result is not evidence about taking GHK (glycyl-L-histidyl-L-lysine).

AI wrote and checked these results. AI can make mistakes, so check important results against the source.

Version history

PepBank keeps every version of the data on this study. Status changes from Retraction Watch, Europe PMC and Crossref are listed with their source.

  1. Version 2Current

    14 Oct 2026, 05:02 UTC

    Study results updated

  2. Version 1

    14 Oct 2026, 01:46 UTC

    First added · source update from Europe PMC

Subjects

Terms as the source lists them.

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