The entire vasoactive intestinal polypeptide molecule is required for the activation of the vasoactive intestinal polypeptide receptor: functional and binding studies on opossum internal anal sphincter smooth muscle.

Chakder S, Rattan S

The Journal of pharmacology and experimental therapeutics1 Jul 1993Cited by 24 (Europe PMC)

PMID8392560DOI10.1016/s0022-3565(25)38347-3PepBankPUB-08392560

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Summary

The text of this summary is not shown here, because the article license does not allow reuse. Read it at the source (opens in a new tab)

Text rights: Publisher copyright. Results still quote short attributed sentences.

Results from this study10

4 results from studies that gave the peptide. No result from this study comes from people.

01LabEx vivo tissueActivity measurement
In opossum internal anal sphincter membranes, binding of radiolabeled VIP to its receptor was specific, saturable and time- and temperature-dependent.
In plain wordsIn lab tests on opossum muscle membranes, VIP bound to its receptor in a specific, saturable way.
Peptide
Vasoactive intestinal peptide (VIP, aviptadil)
Outcome
Not applicableSpecific saturable binding of radiolabeled VIP to its receptor
Study
opossumn: not reported in source
Opossum internal anal sphincter smooth muscle strips and membranes
Outcome group
Receptor activity and mechanism · Gastrointestinal
Studied dose
Dose not stated
Source sentences
Summary
Binding of radiolabeled VIP to its receptor was specific, saturable and time- and temperature-dependent.
Report a problemFND-0839256001
02LabEx vivo tissueActivity measurement
In opossum internal anal sphincter strips, VIP was the most potent substance tested at lowering resting tension.
In plain wordsIn lab tests on opossum muscle strips, VIP lowered resting muscle tension more strongly than the fragments and analogs tested.
Peptide
Vasoactive intestinal peptide (VIP, aviptadil)
Outcome
DecreaseFall in resting tension of internal anal sphincter
Study
opossumn: not reported in source
Opossum internal anal sphincter smooth muscle strips and membranes
Comparator
VIP fragments and analogs
Outcome group
Receptor activity and mechanism · Gastrointestinal
Studied dose
Dose not stated
Source sentences
Summary
VIP was the most potent in causing a fall in the resting tension of the IAS
Report a problemFND-0839256002
03LabEx vivo tissueActivity measurement
In opossum internal anal sphincter membranes, VIP was the most potent substance tested at inhibiting [125I]VIP binding.
In plain wordsIn lab tests on opossum muscle membranes, VIP blocked radiolabeled VIP binding more strongly than the fragments and analogs tested.
Peptide
Vasoactive intestinal peptide (VIP, aviptadil)
Outcome
DecreaseInhibition of [125I]VIP binding
Study
opossumn: not reported in source
Opossum internal anal sphincter smooth muscle strips and membranes
Comparator
VIP fragments and analogs
Outcome group
Receptor activity and mechanism · Gastrointestinal
Studied dose
Dose not stated
Source sentences
Summary
VIP was the most potent in causing a fall in the resting tension of the IAS and inhibiting [125I]VIP binding.
Report a problemFND-0839256003
04LabEx vivo tissueActivity measurement
In opossum internal anal sphincter membranes, the IC50 of VIP for inhibiting [125I]VIP binding was 9.6 x 10(-9) M.
In plain wordsIn lab tests on opossum muscle membranes, VIP blocked radiolabeled VIP binding at a very low concentration.
Peptide
Vasoactive intestinal peptide (VIP, aviptadil)
Outcome
Not applicableIC50 for inhibition of [125I]VIP binding
Study
opossumn: not reported in source
Opossum internal anal sphincter smooth muscle strips and membranes
Outcome group
Receptor activity and mechanism · Gastrointestinal
Studied dose
Dose not stated
Measurements
IC50
9.6 x 10(-9) M
inhibition of [125I]VIP binding, opossum IAS membranes
Source sentences
Summary
The IC50 values for VIP, VIP analog, VIP 10-28, VIP 2-28, GRF analog and peptide histidine isoleucine were 9.6 x 10(-9), 1.6 x 10(-7), 5.5 x 10(-7), 6.2 x 10(-7), 1.2 x 10(-6) and 1.2 x 10(-5) M, respectively.
Report a problemFND-0839256004

Studies that did not test the peptide alone6

In this study, Vasoactive intestinal peptide (VIP, aviptadil) was a lab tool or a carrier, part of a mix, or the subject of a lab method. These results are not evidence about taking Vasoactive intestinal peptide (VIP, aviptadil).

05LabNot a test of Vasoactive intestinal peptide (VIP, aviptadil) aloneEx vivo tissueActivity measurement
In opossum internal anal sphincter membranes, the VIP fragment VIP 10-28 inhibited [125I]VIP binding with an IC50 of 5.5 x 10(-7) M.
In plain wordsIn lab tests on opossum muscle membranes, the VIP piece VIP 10-28 blocked VIP binding only at a much higher concentration than VIP.
Peptide
Vasoactive intestinal peptide (VIP, aviptadil)
Outcome
Not applicableIC50 of VIP 10-28 for inhibition of [125I]VIP binding
Study
opossumn: not reported in source
Opossum internal anal sphincter smooth muscle strips and membranes
Outcome group
Receptor activity and mechanism · Gastrointestinal
Measurements
IC50
5.5 x 10(-7) M
VIP 10-28, inhibition of [125I]VIP binding
Source sentences
Summary
The IC50 values for VIP, VIP analog, VIP 10-28, VIP 2-28, GRF analog and peptide histidine isoleucine were 9.6 x 10(-9), 1.6 x 10(-7), 5.5 x 10(-7), 6.2 x 10(-7), 1.2 x 10(-6) and 1.2 x 10(-5) M, respectively.
In this study, Vasoactive intestinal peptide (VIP, aviptadil) was a lab tool or a carrier, part of a mix, or the subject of a lab method. This result is not evidence about taking Vasoactive intestinal peptide (VIP, aviptadil).
Report a problemFND-0839256005
06LabNot a test of Vasoactive intestinal peptide (VIP, aviptadil) aloneEx vivo tissueActivity measurement
In opossum internal anal sphincter membranes, the VIP fragment VIP 2-28 inhibited [125I]VIP binding with an IC50 of 6.2 x 10(-7) M.
In plain wordsIn lab tests on opossum muscle membranes, the VIP piece VIP 2-28 blocked VIP binding only at a much higher concentration than VIP.
Peptide
Vasoactive intestinal peptide (VIP, aviptadil)
Outcome
Not applicableIC50 of VIP 2-28 for inhibition of [125I]VIP binding
Study
opossumn: not reported in source
Opossum internal anal sphincter smooth muscle strips and membranes
Outcome group
Receptor activity and mechanism · Gastrointestinal
Measurements
IC50
6.2 x 10(-7) M
VIP 2-28, inhibition of [125I]VIP binding
Source sentences
Summary
The IC50 values for VIP, VIP analog, VIP 10-28, VIP 2-28, GRF analog and peptide histidine isoleucine were 9.6 x 10(-9), 1.6 x 10(-7), 5.5 x 10(-7), 6.2 x 10(-7), 1.2 x 10(-6) and 1.2 x 10(-5) M, respectively.
In this study, Vasoactive intestinal peptide (VIP, aviptadil) was a lab tool or a carrier, part of a mix, or the subject of a lab method. This result is not evidence about taking Vasoactive intestinal peptide (VIP, aviptadil).
Report a problemFND-0839256006
07LabNot a test of Vasoactive intestinal peptide (VIP, aviptadil) aloneEx vivo tissueActivity measurement
In opossum internal anal sphincter membranes, the VIP fragments VIP 2-28 and VIP 10-28 significantly inhibited [125I]VIP binding.
In plain wordsIn lab tests on opossum muscle membranes, the VIP pieces VIP 2-28 and VIP 10-28 blocked VIP binding.
Peptide
Vasoactive intestinal peptide (VIP, aviptadil)
Outcome
DecreaseInhibition of [125I]VIP binding by VIP 2-28 and VIP 10-28
Study
opossumn: not reported in source
Opossum internal anal sphincter smooth muscle strips and membranes
Outcome group
Receptor activity and mechanism · Gastrointestinal
Source sentences
Summary
VIP 2-28, VIP 10-28 and the putative VIP antagonists (1 x 10(-6) M) [4Cl-D-Phe6,Leu17]VIP (VIP analog) and (N-Ac-Tyr1, D-Phe2)-growth hormone-releasing factor [GRF] (1-29)-NH2 (GRF analog) caused significant inhibition of [125I]VIP binding
In this study, Vasoactive intestinal peptide (VIP, aviptadil) was a lab tool or a carrier, part of a mix, or the subject of a lab method. This result is not evidence about taking Vasoactive intestinal peptide (VIP, aviptadil).
Report a problemFND-0839256007
08LabNot a test of Vasoactive intestinal peptide (VIP, aviptadil) aloneEx vivo tissueActivity measurement
In opossum internal anal sphincter strips, the VIP fragments VIP 2-28 and VIP 10-28 had only a minimal effect on resting tension.
In plain wordsIn lab tests on opossum muscle strips, the VIP pieces VIP 2-28 and VIP 10-28 barely changed resting muscle tension.
Peptide
Vasoactive intestinal peptide (VIP, aviptadil)
Outcome
No significant changeResting tension with VIP 2-28 and VIP 10-28
Study
opossumn: not reported in source
Opossum internal anal sphincter smooth muscle strips and membranes
Outcome group
Receptor activity and mechanism · Gastrointestinal
Source sentences
Summary
but had only minimal effect on the resting tension of the IAS.
In this study, Vasoactive intestinal peptide (VIP, aviptadil) was a lab tool or a carrier, part of a mix, or the subject of a lab method. This result is not evidence about taking Vasoactive intestinal peptide (VIP, aviptadil).
Report a problemFND-0839256008
09LabNot a test of Vasoactive intestinal peptide (VIP, aviptadil) aloneEx vivo tissueActivity measurement
In opossum internal anal sphincter strips, the VIP fragments VIP 9-18 and VIP 1-12 had no significant effect.
In plain wordsIn lab tests on opossum muscle strips, the VIP pieces VIP 9-18 and VIP 1-12 had no clear effect.
Peptide
Vasoactive intestinal peptide (VIP, aviptadil)
Outcome
No significant changeResting tension with VIP 9-18 and VIP 1-12
Study
opossumn: not reported in source
Opossum internal anal sphincter smooth muscle strips and membranes
Outcome group
Receptor activity and mechanism · Gastrointestinal
Source sentences
Summary
VIP 9-18 and VIP 1-12 had neither any significant effect nor inhibition of receptor binding.
In this study, Vasoactive intestinal peptide (VIP, aviptadil) was a lab tool or a carrier, part of a mix, or the subject of a lab method. This result is not evidence about taking Vasoactive intestinal peptide (VIP, aviptadil).
Report a problemFND-0839256009
10LabNot a test of Vasoactive intestinal peptide (VIP, aviptadil) aloneEx vivo tissueActivity measurement
In opossum internal anal sphincter membranes, the VIP fragments VIP 9-18 and VIP 1-12 did not significantly inhibit receptor binding.
In plain wordsIn lab tests on opossum muscle membranes, the VIP pieces VIP 9-18 and VIP 1-12 did not block VIP receptor binding.
Peptide
Vasoactive intestinal peptide (VIP, aviptadil)
Outcome
No significant changeInhibition of VIP receptor binding by VIP 9-18 and VIP 1-12
Study
opossumn: not reported in source
Opossum internal anal sphincter smooth muscle strips and membranes
Outcome group
Receptor activity and mechanism · Gastrointestinal
Source sentences
Summary
VIP 9-18 and VIP 1-12 had neither any significant effect nor inhibition of receptor binding.
In this study, Vasoactive intestinal peptide (VIP, aviptadil) was a lab tool or a carrier, part of a mix, or the subject of a lab method. This result is not evidence about taking Vasoactive intestinal peptide (VIP, aviptadil).
Report a problemFND-0839256010

AI wrote and checked these results. AI can make mistakes, so check important results against the source.

Version history

PepBank keeps every version of the data on this study. Status changes from Retraction Watch, Europe PMC and Crossref are listed with their source.

Versions 3 to 38Current
28 Oct 2026, 02:04 UTC
The same notice was added 36 times, first on 9 Oct 2026, 19:08 UTC. The status did not change between them.
Study results updated
Version 2
9 Oct 2026, 12:41 UTC
Study data updated · source update from Europe PMC
Version 1
9 Oct 2026, 03:13 UTC
First added · source update from Europe PMC