Skip to content

Study · In vitro (cells) · 1993

2 results

Cloning and functional expression of a human neuroendocrine vasoactive intestinal peptide receptor.

Sreedharan SP, Patel DR, Huang JX, Goetzl EJ

Biochemical and biophysical research communications1 Jun 1993Cited by 131 (Europe PMC)

PMID8390245 (opens in a new tab)DOI10.1006/bbrc.1993.1658 (opens in a new tab)PepBankPUB-08390245
Publisher pagePubMedAsk about this study
Jump to 2 results

Summary

The text of this summary is not shown here, because the article license does not allow reuse. Read it at the source (opens in a new tab)

Text rights: Publisher copyright. Results still quote short attributed sentences.

Results from this study2

2 results from studies that gave the peptide. No result from this study comes from people.

  1. 01LabIn vitro (cells)Activity measurement

    In COS-7 and 293 cells expressing a human VIP receptor, VIP showed specific saturable binding with a Kd of 0.8 nM.

    In plain wordsIn lab cells carrying the human VIP receptor, VIP bound to the receptor specifically and strongly.

    Peptide
    Vasoactive intestinal peptide (VIP, aviptadil)
    Outcome
    Not applicableVIP binding to the cloned receptor
    Study

    humann: not reported in source

    COS-7 and 293 cells transfected with a human VIP receptor cDNA
    Outcome group
    Receptor activity and mechanism · Pharmacology
    Studied dose
    Dose not stated

    Measurements

    Kd
    0.8 nM
    transfected COS-7 and 293 cells

    Source sentences

    Summary
    Transfection of the cDNA into COS-7 cells and 293 cells resulted in expression of specific saturable binding of VIP with a Kd of 0.8 nM
    1 context sentence
    Summary · context
    A cDNA was obtained from human HT29 intestinal epithelial cells and found to encode a 457 amino acid, 52 kDa VIP receptor.
  2. 02LabIn vitro (cells)Activity measurement

    In COS-7 and 293 cells expressing a human VIP receptor, VIP increased intracellular cAMP with an EC50 of 1 nM.

    In plain wordsIn lab cells carrying the human VIP receptor, VIP raised cAMP levels inside the cells.

    Peptide
    Vasoactive intestinal peptide (VIP, aviptadil)
    Outcome
    IncreaseVIP-induced intracellular cAMP increase
    Study

    humann: not reported in source

    COS-7 and 293 cells transfected with a human VIP receptor cDNA
    Outcome group
    Receptor activity and mechanism · Pharmacology
    Studied dose
    Dose not stated

    Measurements

    EC50
    1 nM
    cAMP increase in transfected cells

    Source sentences

    Summary
    and induction of increases in intracellular cAMP by VIP with an EC50 of 1 nM
    1 context sentence
    Summary · context
    A cDNA was obtained from human HT29 intestinal epithelial cells and found to encode a 457 amino acid, 52 kDa VIP receptor.

AI wrote and checked these results. AI can make mistakes, so check important results against the source.

Version history

PepBank keeps every version of the data on this study. Status changes from Retraction Watch, Europe PMC and Crossref are listed with their source.

  1. Version 2Current

    12 Oct 2026, 12:58 UTC

    Study results updated

  2. Version 1

    9 Oct 2026, 12:41 UTC

    First added · source update from Europe PMC

Subjects

Terms as the source lists them.

PepBank is a research reference. Talk to a doctor before you use any peptide.