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Study · In vitro (cells) · 1994

8 results

Prolactin-releasing activity of neurohypophysial hormones: structure-function relationship.

Liu JW, Ben-Jonathan N

Endocrinology1 Jan 1994Cited by 22 (Europe PMC)

PMID8275925 (opens in a new tab)DOI10.1210/endo.134.1.8275925 (opens in a new tab)PepBankPUB-08275925
Publisher pagePubMedAsk about this study
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Summary

The text of this summary is not shown here, because the article license does not allow reuse. Read it at the source (opens in a new tab)

Text rights: Publisher copyright. Results still quote short attributed sentences.

Results from this study8

7 results from studies that gave the peptide. No result from this study comes from people.

  1. 01LabIn vitro (cells)Activity measurement

    In cultured anterior pituitary cells, oxytocin at 25 nM increased prolactin release by 110%.

    In plain wordsIn cells, oxytocin at 25 nM raised prolactin release by 110%.

    Peptide
    Oxytocin
    Outcome
    IncreaseProlactin release at 25 nM
    Study

    n: not reported in source

    cultured anterior pituitary cells
    Outcome group
    Pituitary hormones · Endocrine and hormones
    Studied dose
    25 nM · 15 minAs given in this study.

    Measurements

    PRL release increase
    110%
    25 nM oxytocin

    Source sentences

    Summary
    OT at 25, 100, and 400 nM increased PRL release by 110%, 175%, and 270%, respectively; higher concentrations (1600 and 6400 nM) did not cause a further increase in PRL release.
    1 context sentence
    Summary · context
    AP cells were incubated with peptides for 15 min, and medium PRL concentrations were determined by enzyme-linked immunosorbent assay.
  2. 02LabIn vitro (cells)Activity measurement

    In cultured anterior pituitary cells, oxytocin at 100 nM increased prolactin release by 175%.

    In plain wordsIn cells, oxytocin at 100 nM raised prolactin release by 175%.

    Peptide
    Oxytocin
    Outcome
    IncreaseProlactin release at 100 nM
    Study

    n: not reported in source

    cultured anterior pituitary cells
    Outcome group
    Pituitary hormones · Endocrine and hormones
    Studied dose
    100 nM · 15 minAs given in this study.

    Measurements

    PRL release increase
    175%
    100 nM oxytocin

    Source sentences

    Summary
    OT at 25, 100, and 400 nM increased PRL release by 110%, 175%, and 270%, respectively; higher concentrations (1600 and 6400 nM) did not cause a further increase in PRL release.
    1 context sentence
    Summary · context
    AP cells were incubated with peptides for 15 min, and medium PRL concentrations were determined by enzyme-linked immunosorbent assay.
  3. 03LabIn vitro (cells)Activity measurement

    In cultured anterior pituitary cells, oxytocin at 400 nM increased prolactin release by 270%.

    In plain wordsIn cells, oxytocin at 400 nM raised prolactin release by 270%.

    Peptide
    Oxytocin
    Outcome
    IncreaseProlactin release at 400 nM
    Study

    n: not reported in source

    cultured anterior pituitary cells
    Outcome group
    Pituitary hormones · Endocrine and hormones
    Studied dose
    400 nM · 15 minAs given in this study.

    Measurements

    PRL release increase
    270%
    400 nM oxytocin

    Source sentences

    Summary
    OT at 25, 100, and 400 nM increased PRL release by 110%, 175%, and 270%, respectively; higher concentrations (1600 and 6400 nM) did not cause a further increase in PRL release.
    1 context sentence
    Summary · context
    AP cells were incubated with peptides for 15 min, and medium PRL concentrations were determined by enzyme-linked immunosorbent assay.
  4. 04LabIn vitro (cells)Activity measurement

    In cultured anterior pituitary cells, oxytocin at 1600 and 6400 nM caused no further increase in prolactin release.

    In plain wordsIn cells, oxytocin at very high doses did not raise prolactin release further.

    Peptide
    Oxytocin
    Outcome
    No significant changeProlactin release at 1600 and 6400 nM
    Study

    n: not reported in source

    cultured anterior pituitary cells
    Outcome group
    Pituitary hormones · Endocrine and hormones
    Studied dose
    1600 and 6400 nM · 15 minAs given in this study.

    Measurements

    concentration
    1600 to 6400 nM
    no further PRL increase

    Source sentences

    Summary
    higher concentrations (1600 and 6400 nM) did not cause a further increase in PRL release.
    1 context sentence
    Summary · context
    AP cells were incubated with peptides for 15 min, and medium PRL concentrations were determined by enzyme-linked immunosorbent assay.
  5. 05LabIn vitro (cells)Activity measurement

    In cultured anterior pituitary cells, TRH was 5-10 times more potent than oxytocin at releasing prolactin on a molar basis.

    In plain wordsIn cells, TRH released prolactin at 5-10 times lower doses than oxytocin needed.

    Peptide
    Oxytocin
    Outcome
    Not applicableProlactin-releasing potency relative to TRH
    Study

    n: not reported in source

    cultured anterior pituitary cells
    Comparator
    TRH
    Outcome group
    Pituitary hormones · Endocrine and hormones
    Studied dose
    Dose not stated · 15 minAs given in this study.

    Measurements

    potency ratio TRH to OT
    5 to 10 fold
    molar basis

    Source sentences

    Summary
    TRH was 5-10 times more potent than OT on a molar basis.
    1 context sentence
    Summary · context
    AP cells were incubated with peptides for 15 min, and medium PRL concentrations were determined by enzyme-linked immunosorbent assay.
  6. 06LabIn vitro (cells)Activity measurement

    GH3 tumour cells did not respond to oxytocin.

    In plain wordsIn lab-grown tumour cells of the GH3 line, oxytocin caused no prolactin response.

    Peptide
    Oxytocin
    Outcome
    No significant changeProlactin release in GH3 cells
    Study

    n: not reported in source

    GH3 somatommamotroph tumour cell line
    Outcome group
    Pituitary hormones · Endocrine and hormones
    Studied dose
    Dose not stated · 15 minAs given in this study.

    Source sentences

    Summary
    GH3 cells, a somatommamotroph tumor cell line, did not respond to OT and related compounds, but showed a similar responsiveness to TRH as AP cells.
    1 context sentence
    Summary · context
    AP cells were incubated with peptides for 15 min, and medium PRL concentrations were determined by enzyme-linked immunosorbent assay.
  7. 07LabIn vitro (cells)Activity measurement

    In cultured anterior pituitary cells, oxytocin and arginine vasotocin had the highest prolactin-releasing activity among twelve neurohypophysial peptides and analogs.

    In plain wordsIn cells, oxytocin and arginine vasotocin released more prolactin than the other related peptides and analogs tested.

    Peptide
    Oxytocin
    Outcome
    IncreaseRelative prolactin-releasing activity among 12 neurohypophysial peptides
    Study

    n: not reported in source

    cultured anterior pituitary cells
    Comparator
    Eleven other neurohypophysial peptides and analogs
    Outcome group
    Pituitary hormones · Endocrine and hormones
    Studied dose
    Dose not stated · 15 minAs given in this study.

    Source sentences

    Summary
    OT and arginine vasotocin (AVT) showed the highest PRL-releasing activity.
    2 context sentences
    Summary · context
    Twelve neurohypophysial peptides and selected analogs were incubated with AP cells, and their relative PRL-releasing activities were compared.
    Summary · context
    AP cells were incubated with peptides for 15 min, and medium PRL concentrations were determined by enzyme-linked immunosorbent assay.

Studies that did not test the peptide alone1

In this study, Oxytocin was a lab tool or a carrier, part of a mix, or the subject of a lab method. These results are not evidence about taking Oxytocin.

  1. 08LabNot a test of Oxytocin aloneIn vitro (cells)Mechanism

    In cultured anterior pituitary cells, coincubation with a specific oxytocin receptor antagonist abolished the prolactin-releasing activity of oxytocin.

    In plain wordsIn cells, an oxytocin receptor blocker stopped oxytocin from releasing prolactin.

    Peptide
    Oxytocin
    Outcome
    DecreaseOxytocin-induced prolactin release with an oxytocin receptor antagonist
    Study

    n: not reported in source

    cultured anterior pituitary cells
    Comparator
    Oxytocin alone
    Outcome group
    Pituitary hormones · Endocrine and hormones

    Source sentences

    Summary
    Coincubation of OT, AVT, or AVP with a specific OT receptor antagonist abolished their PRL-releasing activity.
    1 context sentence
    Summary · context
    AP cells were incubated with peptides for 15 min, and medium PRL concentrations were determined by enzyme-linked immunosorbent assay.

    In this study, Oxytocin was a lab tool or a carrier, part of a mix, or the subject of a lab method. This result is not evidence about taking Oxytocin.

AI wrote and checked these results. AI can make mistakes, so check important results against the source.

Version history

PepBank keeps every version of the data on this study. Status changes from Retraction Watch, Europe PMC and Crossref are listed with their source.

  1. Version 2Current

    11 Oct 2026, 20:03 UTC

    Study results updated

  2. Version 1

    9 Oct 2026, 02:52 UTC

    First added · source update from Europe PMC

Subjects

Terms as the source lists them.

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