Expression of functional receptors for vasoactive intestinal peptide in freshly isolated and cultured gastric muscle cells.

Chijiiwa Y, Murthy KS, Grider JR, Makhlouf GM

Regulatory peptides1 Sept 1993Cited by 18 (Europe PMC)

PMID8234906DOI10.1016/0167-0115(93)90389-pPepBankPUB-08234906

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Summary

The text of this summary is not shown here, because the article license does not allow reuse. Read it at the source (opens in a new tab)

Text rights: Publisher copyright. Results still quote short attributed sentences.

Results from this study3

2 results from studies that gave the peptide. No result from this study comes from people.

01LabIn vitro (cells)Activity measurement
In freshly isolated and cultured guinea pig gastric muscle cells, 125I-VIP binding was rapid, specific, saturable and temperature-dependent.
In plain wordsIn cells from guinea pig stomach, VIP bound quickly and specifically to the cells, and the binding could be saturated.
Peptide
Vasoactive intestinal peptide (VIP, aviptadil)
Outcome
Not applicable125I-VIP binding to gastric muscle cells
Study
guinea pign: not reported in source
freshly isolated and cultured guinea pig gastric smooth muscle cells
Outcome group
Receptor activity and mechanism · Gastrointestinal
Studied dose
Dose not stated
Source sentences
Summary
125I-VIP bound to both freshly isolated and cultured muscle cells: binding was rapid, specific, saturable and temperature-dependent
1 context sentence(design, population, supporting numbers)
Summary · context
Vasoactive intestinal peptide (VIP) receptors were characterized in freshly isolated and cultured smooth muscle cells from guinea pig stomach by radioligand binding and by measurement of relaxation in single isolated and cultured cells.
Report a problemFND-0823490601
02LabIn vitro (cells)Activity measurement
In guinea pig gastric muscle cells, VIP competition curves showed high- and low-affinity components with similar binding constants in freshly isolated and cultured cells.
In plain wordsIn cells from guinea pig stomach, VIP bound to two kinds of sites, with similar values in fresh and cultured cells.
Peptide
Vasoactive intestinal peptide (VIP, aviptadil)
Outcome
Not applicableVIP binding affinity and binding site number
Study
guinea pign: not reported in source
freshly isolated and cultured guinea pig gastric smooth muscle cells
Outcome group
Receptor activity and mechanism · Gastrointestinal
Studied dose
Dose not stated
Measurements
high-affinity Kd
0.11 nM
freshly isolated cells
high-affinity Kd
0.22 nM
cultured cells
low-affinity Kd
59 nM
freshly isolated cells
low-affinity Kd
37 nM
cultured cells
high-affinity binding sites
1183 per cell
freshly isolated cells
high-affinity binding sites
1021 per cell
cultured cells
Source sentences
Summary
Competition curves for VIP could be resolved into high- and low-affinity components, yielding similar binding constants in freshly isolated and cultured cells (high-affinity Kd 0.11 and 0.22 nM; low-affinity Kd 59 and 37 nM; high-affinity binding sites: 1183 and 1021 per cell, representing about 1%…
1 context sentence(design, population, supporting numbers)
Summary · context
Vasoactive intestinal peptide (VIP) receptors were characterized in freshly isolated and cultured smooth muscle cells from guinea pig stomach by radioligand binding and by measurement of relaxation in single isolated and cultured cells.
Report a problemFND-0823490602

Studies that did not test the peptide alone1

In this study, Vasoactive intestinal peptide (VIP, aviptadil) was a lab tool or a carrier, part of a mix, or the subject of a lab method. These results are not evidence about taking Vasoactive intestinal peptide (VIP, aviptadil).

03LabNot a test of Vasoactive intestinal peptide (VIP, aviptadil) aloneIn vitro (cells)Activity measurement
In guinea pig gastric muscle cells, VIP10-28 completely inhibited 125I-VIP binding and acted as a competitive antagonist of VIP-induced relaxation.
In plain wordsIn cells from guinea pig stomach, the fragment VIP10-28 blocked VIP binding and VIP-driven relaxation.
Peptide
Vasoactive intestinal peptide (VIP, aviptadil)
Outcome
Not applicableVIP10-28 antagonism of 125I-VIP binding and VIP-induced relaxation
Study
guinea pign: not reported in source
freshly isolated and cultured guinea pig gastric smooth muscle cells
Outcome group
Receptor activity and mechanism · Gastrointestinal
Measurements
Ki
0.5 nM
VIP-induced relaxation
Source sentences
Summary
VIP10-28 inhibited 125I-VIP binding completely and acted as potent competitive antagonist of VIP-induced relaxation (Ki 0.5 nM).
1 context sentence(design, population, supporting numbers)
Summary · context
Vasoactive intestinal peptide (VIP) receptors were characterized in freshly isolated and cultured smooth muscle cells from guinea pig stomach by radioligand binding and by measurement of relaxation in single isolated and cultured cells.
In this study, Vasoactive intestinal peptide (VIP, aviptadil) was a lab tool or a carrier, part of a mix, or the subject of a lab method. This result is not evidence about taking Vasoactive intestinal peptide (VIP, aviptadil).
Report a problemFND-0823490603

AI wrote and checked these results. AI can make mistakes, so check important results against the source.

Version history

PepBank keeps every version of the data on this study. Status changes from Retraction Watch, Europe PMC and Crossref are listed with their source.

Version 2Current
12 Oct 2026, 12:58 UTC
Study results updated
Version 1
9 Oct 2026, 12:41 UTC
First added · source update from Europe PMC

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