GHRP-6 induces a biphasic calcium response in rat pituitary somatotrophs.

Bresson-Bépoldin L, Dufy-Barbe L

Cell calcium1 Mar 1994Cited by 27 (Europe PMC)

PMID8194104DOI10.1016/0143-4160(94)90064-7PepBankPUB-08194104

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Summary

The text of this summary is not shown here, because the article license does not allow reuse. Read it at the source (opens in a new tab)

Text rights: Publisher copyright. Results still quote short attributed sentences.

Results from this study9

2 results from studies that gave the peptide. No result from this study comes from people.

01LabIn vitro (cells)Mechanism
In rat somatotrophs, brief GHRP‑6 application caused a biphasic calcium response in 44% of cells.
In plain wordsIn cells from rats, GHRP‑6 caused a two-phase calcium increase in 44% of cells.
Peptide
GHRP-6
Outcome
IncreaseBiphasic calcium response
Study
ratn: not reported in source
primary somatotroph cultures
Outcome group
Receptor activity and mechanism · Endocrine and hormones
Studied dose
10(-5) M · 10 sAs given in this study (rat).
Measurements
Responding cells
44%
biphasic response
Source sentences
Summary
A short application of GHRP-6 (10(-5) M, 10 s) induced a biphasic Ca2+ response in most cells (44%), which consisted in a rapid and large rise in [Ca2+]i followed by sustained oscillations.
1 context sentence(design, population, supporting numbers)
Summary · context
We have studied the effects of GHRP-6 on the cytosolic free calcium concentration ([Ca2+]i) of somatotrophs in primary culture.
Report a problemFND-0819410401
02LabIn vitro (cells)Mechanism
In rat somatotrophs, the GHRP‑6 calcium response was dose dependent.
In plain wordsIn cells from rats, the GHRP‑6 calcium response depended on concentration.
Peptide
GHRP-6
Outcome
IncreaseCalcium dose response
Study
ratn: not reported in source
primary somatotroph cultures
Outcome group
Receptor activity and mechanism · Endocrine and hormones
Studied dose
10(-10) to 10(-5) MAs given in this study (rat).
Source sentences
Summary
This response is dose dependent in a range of concentrations from 10(-10) to 10(-5) M.
2 context sentences(design, population, supporting numbers)
Summary · context
A short application of GHRP-6 (10(-5) M, 10 s) induced a biphasic Ca2+ response in most cells (44%), which consisted in a rapid and large rise in [Ca2+]i followed by sustained oscillations.
Summary · context
We have studied the effects of GHRP-6 on the cytosolic free calcium concentration ([Ca2+]i) of somatotrophs in primary culture.
Report a problemFND-0819410402

Studies that did not test the peptide alone7

In this study, GHRP-6 was a lab tool or a carrier, part of a mix, or the subject of a lab method. These results are not evidence about taking GHRP-6.

03LabNot a test of GHRP-6 aloneIn vitro (cells)Mechanism
In rat somatotrophs, extracellular calcium removal preserved the first GHRP‑6 calcium response phase.
In plain wordsIn cells from rats, removing external calcium preserved the first GHRP‑6 calcium response phase.
Peptide
GHRP-6
Outcome
No significant changeFirst calcium phase without extracellular calcium
Study
ratn: not reported in source
primary somatotroph cultures
Outcome group
Receptor activity and mechanism · Endocrine and hormones
Source sentences
Summary
The first phase of the GHRP-6 response persisted in the absence of Ca2+ in the extracellular medium, whereas the second phase was inhibited.
1 context sentence(design, population, supporting numbers)
Summary · context
We have studied the effects of GHRP-6 on the cytosolic free calcium concentration ([Ca2+]i) of somatotrophs in primary culture.
In this study, GHRP-6 was a lab tool or a carrier, part of a mix, or the subject of a lab method. This result is not evidence about taking GHRP-6.
Report a problemFND-0819410403
04LabNot a test of GHRP-6 aloneIn vitro (cells)Mechanism
In rat somatotrophs, extracellular calcium removal inhibited the second GHRP‑6 calcium response phase.
In plain wordsIn cells from rats, removing external calcium reduced the second GHRP‑6 calcium response phase.
Peptide
GHRP-6
Outcome
DecreaseSecond calcium phase without extracellular calcium
Study
ratn: not reported in source
primary somatotroph cultures
Outcome group
Receptor activity and mechanism · Endocrine and hormones
Source sentences
Summary
The first phase of the GHRP-6 response persisted in the absence of Ca2+ in the extracellular medium, whereas the second phase was inhibited.
1 context sentence(design, population, supporting numbers)
Summary · context
We have studied the effects of GHRP-6 on the cytosolic free calcium concentration ([Ca2+]i) of somatotrophs in primary culture.
In this study, GHRP-6 was a lab tool or a carrier, part of a mix, or the subject of a lab method. This result is not evidence about taking GHRP-6.
Report a problemFND-0819410404
05LabNot a test of GHRP-6 aloneIn vitro (cells)Mechanism
In rat somatotrophs, calcium channel blockers prevented the second GHRP‑6 calcium response phase.
In plain wordsIn cells from rats, calcium channel blockers prevented the second GHRP‑6 calcium response phase.
Peptide
GHRP-6
Outcome
DecreaseSecond calcium phase under channel blockade
Study
ratn: not reported in source
primary somatotroph cultures
Outcome group
Receptor activity and mechanism · Endocrine and hormones
Source sentences
Summary
The application of Ca2+ channel blockers like cadmium chloride (200 microM) or PN-200-110 (200 nM) also prevented the second phase.
1 context sentence(design, population, supporting numbers)
Summary · context
We have studied the effects of GHRP-6 on the cytosolic free calcium concentration ([Ca2+]i) of somatotrophs in primary culture.
In this study, GHRP-6 was a lab tool or a carrier, part of a mix, or the subject of a lab method. This result is not evidence about taking GHRP-6.
Report a problemFND-0819410405
06LabNot a test of GHRP-6 aloneIn vitro (cells)Mechanism
In rat somatotrophs, thapsigargin abolished the first GHRP‑6 calcium response phase.
In plain wordsIn cells from rats, thapsigargin stopped the first GHRP‑6 calcium response phase.
Peptide
GHRP-6
Outcome
DecreaseFirst calcium phase after thapsigargin
Study
ratn: not reported in source
primary somatotroph cultures
Outcome group
Receptor activity and mechanism · Endocrine and hormones
Source sentences
Summary
Conversely, when the cells were pretreated with thapsigargin (TG) (100 nM), the first phase of the GHRP-6 Ca2+ response was abolished, whereas the second phase alone was preserved.
1 context sentence(design, population, supporting numbers)
Summary · context
We have studied the effects of GHRP-6 on the cytosolic free calcium concentration ([Ca2+]i) of somatotrophs in primary culture.
In this study, GHRP-6 was a lab tool or a carrier, part of a mix, or the subject of a lab method. This result is not evidence about taking GHRP-6.
Report a problemFND-0819410406
07LabNot a test of GHRP-6 aloneIn vitro (cells)Mechanism
In rat somatotrophs, thapsigargin preserved the second GHRP‑6 calcium response phase.
In plain wordsIn cells from rats, thapsigargin preserved the second GHRP‑6 calcium response phase.
Peptide
GHRP-6
Outcome
No significant changeSecond calcium phase after thapsigargin
Study
ratn: not reported in source
primary somatotroph cultures
Outcome group
Receptor activity and mechanism · Endocrine and hormones
Source sentences
Summary
Conversely, when the cells were pretreated with thapsigargin (TG) (100 nM), the first phase of the GHRP-6 Ca2+ response was abolished, whereas the second phase alone was preserved.
1 context sentence(design, population, supporting numbers)
Summary · context
We have studied the effects of GHRP-6 on the cytosolic free calcium concentration ([Ca2+]i) of somatotrophs in primary culture.
In this study, GHRP-6 was a lab tool or a carrier, part of a mix, or the subject of a lab method. This result is not evidence about taking GHRP-6.
Report a problemFND-0819410407
08LabNot a test of GHRP-6 aloneIn vitro (cells)Mechanism
In rat somatotrophs, PKC depletion inhibited the second GHRP‑6 calcium response phase; phloretin corroborated this result.
In plain wordsIn cells from rats, reducing PKC activity inhibited the second GHRP‑6 calcium response phase.
Peptide
GHRP-6
Outcome
DecreaseSecond calcium phase after PKC depletion
Study
ratn: not reported in source
primary somatotroph cultures
Outcome group
Receptor activity and mechanism · Endocrine and hormones
Source sentences
Summary
When the cells were depleted in PKC by incubation with 10(-6) M PMA for 24 h, the second phase of the GHRP-6 response was inhibited, and only the first phase was maintained.
2 context sentences(design, population, supporting numbers)
Summary · context
These results were corroborated by using phloretin, a PKC inhibitor.
Summary · context
We have studied the effects of GHRP-6 on the cytosolic free calcium concentration ([Ca2+]i) of somatotrophs in primary culture.
In this study, GHRP-6 was a lab tool or a carrier, part of a mix, or the subject of a lab method. This result is not evidence about taking GHRP-6.
Report a problemFND-0819410408
09LabNot a test of GHRP-6 aloneIn vitro (cells)Mechanism
In rat somatotrophs, PKC depletion preserved the first GHRP‑6 calcium response phase.
In plain wordsIn cells from rats, reducing PKC activity preserved the first GHRP‑6 calcium response phase.
Peptide
GHRP-6
Outcome
No significant changeFirst calcium phase after PKC depletion
Study
ratn: not reported in source
primary somatotroph cultures
Outcome group
Receptor activity and mechanism · Endocrine and hormones
Source sentences
Summary
When the cells were depleted in PKC by incubation with 10(-6) M PMA for 24 h, the second phase of the GHRP-6 response was inhibited, and only the first phase was maintained.
1 context sentence(design, population, supporting numbers)
Summary · context
We have studied the effects of GHRP-6 on the cytosolic free calcium concentration ([Ca2+]i) of somatotrophs in primary culture.
In this study, GHRP-6 was a lab tool or a carrier, part of a mix, or the subject of a lab method. This result is not evidence about taking GHRP-6.
Report a problemFND-0819410409

AI wrote and checked these results. AI can make mistakes, so check important results against the source.

Version history

PepBank keeps every version of the data on this study. Status changes from Retraction Watch, Europe PMC and Crossref are listed with their source.

Version 2Current
21 Oct 2026, 15:58 UTC
Study results updated
Version 1
9 Oct 2026, 03:13 UTC
First added · source update from Europe PMC

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