Cellular mechanisms mediating the stimulation of ovine endometrial secretion of prostaglandin F2 alpha in response to oxytocin: role of phospholipase A2.

Lee JS, Silvia WJ

The Journal of endocrinology1 Jun 1994Cited by 30 (Europe PMC)

PMID8071648DOI10.1677/joe.0.1410491PepBankPUB-08071648

Publisher page PubMedAsk about this study

Summary

The text of this summary is not shown here, because the article license does not allow reuse. Read it at the source (opens in a new tab)

Text rights: Publisher copyright. Results still quote short attributed sentences.

Results from this study5

2 results from studies that gave the peptide. No result from this study comes from people.

01LabEx vivo tissueMechanism
In ovine endometrial explants, oxytocin stimulated the release of PGF2 alpha.
In plain wordsIn lab tests on sheep womb tissue, oxytocin raised the release of prostaglandin F2 alpha.
Peptide
Oxytocin
Outcome
IncreasePGF2 alpha release after oxytocin (experiment 1)
Study
sheepn: not reported in source
ovine caruncular endometrial explants
Outcome group
Receptor activity and mechanism · Reproductive and sexual health
Studied dose
10(-7) M · in vitro (explant incubation)As given in this study (sheep).
Source sentences
Summary
In experiment 1, oxytocin (10(-7) M) and melittin (1.76 x 10(-6) M; a stimulator of PLA2) stimulated release of PGF2 alpha from tissue explants (P < 0.05).
1 context sentence(design, population, supporting numbers)
Summary · context
Four experiments were conducted to determine whether phospholipase (PL) A2 mediates the stimulatory effect of oxytocin on the release of prostaglandin (PG) F2 alpha from ovine endometrial tissue. Caruncular endometrial tissue was collected from ovariectomized ewes on the day after a steroid replacem…
Report a problemFND-0807164801
02LabEx vivo tissueMechanism
In ovine endometrial explants, oxytocin stimulated the release of PGF2 alpha, as did AlF4-, melittin and arachidonic acid.
In plain wordsIn lab tests on sheep womb tissue, oxytocin raised prostaglandin F2 alpha release, as did three other stimulators.
Peptide
Oxytocin
Outcome
IncreasePGF2 alpha release after oxytocin (experiment 3)
Study
sheepn: not reported in source
ovine caruncular endometrial explants
Outcome group
Receptor activity and mechanism · Reproductive and sexual health
Studied dose
10(-7) M · in vitro (explant incubation)As given in this study (sheep).
Source sentences
Summary
Oxytocin (10(-7) M), AlF4- (5 x 10(-2) M NaF, 10(-5) M AlCl3), melittin (1.76 x 10(-6) M) and arachidonic acid (AA; 20 micrograms/ml) stimulated release of PGF2 alpha (P < 0.05).
1 context sentence(design, population, supporting numbers)
Summary · context
Four experiments were conducted to determine whether phospholipase (PL) A2 mediates the stimulatory effect of oxytocin on the release of prostaglandin (PG) F2 alpha from ovine endometrial tissue. Caruncular endometrial tissue was collected from ovariectomized ewes on the day after a steroid replacem…
Report a problemFND-0807164802

Studies that did not test the peptide alone3

In this study, Oxytocin was a lab tool or a carrier, part of a mix, or the subject of a lab method. These results are not evidence about taking Oxytocin.

03LabNot a test of Oxytocin aloneEx vivo tissueMechanism
In ovine endometrial explants, the PLA2 inhibitor aristolochic acid decreased oxytocin-induced PGF2 alpha release by 77%.
In plain wordsIn lab tests on sheep womb tissue, a phospholipase A2 blocker cut oxytocin-induced prostaglandin F2 alpha release.
Peptide
Oxytocin
Outcome
DecreaseAristolochic acid effect on oxytocin-induced PGF2 alpha release
Study
sheepn: not reported in source
ovine caruncular endometrial explants
Outcome group
Receptor activity and mechanism · Reproductive and sexual health
Measurements
Reduction of oxytocin-induced PGF2 alpha release
77%
aristolochic acid 10(-4) M
Source sentences
Summary
Aristolochic acid (10(-4) M; an inhibitor of PLA2) decreased oxytocin- and melittin-induced release of PGF2 alpha by 77% and 71% respectively (P < 0.05).
1 context sentence(design, population, supporting numbers)
Summary · context
Four experiments were conducted to determine whether phospholipase (PL) A2 mediates the stimulatory effect of oxytocin on the release of prostaglandin (PG) F2 alpha from ovine endometrial tissue. Caruncular endometrial tissue was collected from ovariectomized ewes on the day after a steroid replacem…
In this study, Oxytocin was a lab tool or a carrier, part of a mix, or the subject of a lab method. This result is not evidence about taking Oxytocin.
Report a problemFND-0807164803
04LabNot a test of Oxytocin aloneEx vivo tissueMechanism
In ovine endometrial explants, 10(-5) M aristolochic acid inhibited basal PGF2 alpha release, but 10(-4) M was needed to block the oxytocin stimulation.
In plain wordsIn lab tests on sheep womb tissue, blocking the oxytocin effect on prostaglandin release needed a higher dose of the blocker than basal release did.
Peptide
Oxytocin
Outcome
DecreaseAristolochic acid dose needed to block oxytocin effect
Study
sheepn: not reported in source
ovine caruncular endometrial explants
Outcome group
Receptor activity and mechanism · Reproductive and sexual health
Source sentences
Summary
Experiment 2 was conducted to establish the minimum inhibitory dose of aristolochic acid. Basal release of PGF2 alpha was inhibited at 10(-5) M aristolochic acid, but 10(-4) M was required to block the stimulatory effect of oxytocin.
1 context sentence(design, population, supporting numbers)
Summary · context
Four experiments were conducted to determine whether phospholipase (PL) A2 mediates the stimulatory effect of oxytocin on the release of prostaglandin (PG) F2 alpha from ovine endometrial tissue. Caruncular endometrial tissue was collected from ovariectomized ewes on the day after a steroid replacem…
In this study, Oxytocin was a lab tool or a carrier, part of a mix, or the subject of a lab method. This result is not evidence about taking Oxytocin.
Report a problemFND-0807164804
05LabNot a test of Oxytocin aloneEx vivo tissueMechanism
In ovine endometrial explants, aristolochic acid blocked PGF2 alpha release stimulated by oxytocin, AlF4- or melittin by more than 80%.
In plain wordsIn lab tests on sheep womb tissue, a phospholipase A2 blocker stopped most prostaglandin F2 alpha release caused by oxytocin and two other stimulators.
Peptide
Oxytocin
Outcome
DecreaseAristolochic acid effect on PGF2 alpha release stimulated by oxytocin, AlF4- or melittin
Study
sheepn: not reported in source
ovine caruncular endometrial explants
Outcome group
Receptor activity and mechanism · Reproductive and sexual health
Source sentences
Summary
Aristolochic acid (10(-4) M) blocked the release of PGF2 alpha stimulated by oxytocin, AlF4- or melittin by > 80% (P < 0.05).(ABSTRACT TRUNCATED AT 250 WORDS)
1 context sentence(design, population, supporting numbers)
Summary · context
Four experiments were conducted to determine whether phospholipase (PL) A2 mediates the stimulatory effect of oxytocin on the release of prostaglandin (PG) F2 alpha from ovine endometrial tissue. Caruncular endometrial tissue was collected from ovariectomized ewes on the day after a steroid replacem…
In this study, Oxytocin was a lab tool or a carrier, part of a mix, or the subject of a lab method. This result is not evidence about taking Oxytocin.
Report a problemFND-0807164805

AI wrote and checked these results. AI can make mistakes, so check important results against the source.

Version history

PepBank keeps every version of the data on this study. Status changes from Retraction Watch, Europe PMC and Crossref are listed with their source.

Version 2Current
11 Oct 2026, 20:03 UTC
Study results updated
Version 1
9 Oct 2026, 02:51 UTC
First added · source update from Europe PMC

Subjects

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