Cellular mechanisms mediating the stimulation of ovine endometrial secretion of prostaglandin F2 alpha in response to oxytocin: role of phospholipase C and diacylglycerol.

Silvia WJ, Lee JS, Trammell DS, Hayes SH, Lowberger LL, Brockman JA

The Journal of endocrinology1 Jun 1994Cited by 17 (Europe PMC)

PMID8071647DOI10.1677/joe.0.1410481PepBankPUB-08071647

Publisher page PubMedAsk about this study

Summary

The text of this summary is not shown here, because the article license does not allow reuse. Read it at the source (opens in a new tab)

Text rights: Publisher copyright. Results still quote short attributed sentences.

Results from this study10

8 results from studies that gave the peptide. No result from this study comes from people.

01LabEx vivo tissueMechanism
In ovine endometrial explants, oxytocin increased phospholipase C activity, first detected at 3 min.
In plain wordsIn lab tests on sheep womb tissue, oxytocin raised phospholipase C activity within 3 minutes.
Peptide
Oxytocin
Outcome
IncreasePLC activity time-course after oxytocin
Study
sheepn: not reported in source
ovine caruncular endometrial explants
Outcome group
Receptor activity and mechanism · Reproductive and sexual health
Studied dose
10(-6) M · in vitro (explant incubation) · 0-100 minAs given in this study (sheep).
Measurements
Time to detectable PLC increase
3 min
oxytocin 10(-6) M
Source sentences
Summary
In experiment 1, explants were incubated either in the presence (10(-6) M) or absence of oxytocin for 0, 1, 3, 10, 30 or 100 min to examine the time-course for activation of PLC and release of PGF2 alpha in response to oxytocin. An increase in the activity of PLC was detected at 3 min while an incre…
1 context sentence(design, population, supporting numbers)
Summary · context
Caruncular endometrial tissue was collected from ovariectomized ewes after completion of an 11-day steroid replacement protocol.
Report a problemFND-0807164701
02LabEx vivo tissueMechanism
In ovine endometrial explants, oxytocin increased PGF2 alpha release, first detected at 10 min, later than the rise in PLC activity.
In plain wordsIn lab tests on sheep womb tissue, oxytocin raised prostaglandin F2 alpha release after 10 minutes, later than the enzyme rise.
Peptide
Oxytocin
Outcome
IncreasePGF2 alpha release time-course after oxytocin
Study
sheepn: not reported in source
ovine caruncular endometrial explants
Outcome group
Receptor activity and mechanism · Reproductive and sexual health
Studied dose
10(-6) M · in vitro (explant incubation) · 0-100 minAs given in this study (sheep).
Measurements
Time to detectable PGF2 alpha increase
10 min
oxytocin 10(-6) M
Source sentences
Summary
In experiment 1, explants were incubated either in the presence (10(-6) M) or absence of oxytocin for 0, 1, 3, 10, 30 or 100 min to examine the time-course for activation of PLC and release of PGF2 alpha in response to oxytocin. An increase in the activity of PLC was detected at 3 min while an incre…
1 context sentence(design, population, supporting numbers)
Summary · context
Caruncular endometrial tissue was collected from ovariectomized ewes after completion of an 11-day steroid replacement protocol.
Report a problemFND-0807164702
03LabEx vivo tissueMechanism
In ovine endometrial explants, oxytocin stimulated phospholipase C activity.
In plain wordsIn lab tests on sheep womb tissue, oxytocin raised phospholipase C activity.
Peptide
Oxytocin
Outcome
IncreasePLC activity after oxytocin (experiment 2)
Study
sheepn: not reported in source
ovine caruncular endometrial explants
Outcome group
Receptor activity and mechanism · Reproductive and sexual health
Studied dose
10(-6) M · in vitro (explant incubation)As given in this study (sheep).
Source sentences
Summary
In experiment 2, explants were incubated in the presence of various oxytocin analogues (10(-6) M) to compare their abilities to activate PLC and release PGF2 alpha. Oxytocin and three receptor agonists stimulated the activity of PLC and the release of PGF2 alpha (P < 0.05) while two oxytocin recepto…
1 context sentence(design, population, supporting numbers)
Summary · context
Caruncular endometrial tissue was collected from ovariectomized ewes after completion of an 11-day steroid replacement protocol.
Report a problemFND-0807164703
04LabEx vivo tissueMechanism
In ovine endometrial explants, oxytocin stimulated the release of PGF2 alpha.
In plain wordsIn lab tests on sheep womb tissue, oxytocin raised the release of prostaglandin F2 alpha.
Peptide
Oxytocin
Outcome
IncreasePGF2 alpha release after oxytocin (experiment 2)
Study
sheepn: not reported in source
ovine caruncular endometrial explants
Outcome group
Receptor activity and mechanism · Reproductive and sexual health
Studied dose
10(-6) M · in vitro (explant incubation)As given in this study (sheep).
Source sentences
Summary
In experiment 2, explants were incubated in the presence of various oxytocin analogues (10(-6) M) to compare their abilities to activate PLC and release PGF2 alpha. Oxytocin and three receptor agonists stimulated the activity of PLC and the release of PGF2 alpha (P < 0.05) while two oxytocin recepto…
1 context sentence(design, population, supporting numbers)
Summary · context
Caruncular endometrial tissue was collected from ovariectomized ewes after completion of an 11-day steroid replacement protocol.
Report a problemFND-0807164704
05LabEx vivo tissueMechanism
In ovine endometrial explants, oxytocin significantly increased PGF2 alpha release from 10(-8) M.
In plain wordsIn lab tests on sheep womb tissue, oxytocin raised prostaglandin F2 alpha release from a dose of 10(-8) M.
Peptide
Oxytocin
Outcome
IncreasePGF2 alpha release dose-response to oxytocin
Study
sheepn: not reported in source
ovine caruncular endometrial explants
Outcome group
Receptor activity and mechanism · Reproductive and sexual health
Studied dose
10(-9) to 10(-6) M · in vitro (explant incubation)As given in this study (sheep).
Source sentences
Summary
In experiment 3, explants were incubated in the presence of oxytocin or arginine vasopressin at 10(-9) to 10(-6) M to establish dose-response curves for the activation of PLC and release of PGF2 alpha. For both hormones, significant increases (P < 0.05) in the release of PGF2 alpha were observed at…
1 context sentence(design, population, supporting numbers)
Summary · context
Caruncular endometrial tissue was collected from ovariectomized ewes after completion of an 11-day steroid replacement protocol.
Report a problemFND-0807164705
06LabEx vivo tissueMechanism
In ovine endometrial explants, oxytocin increased PLC activity only from 10(-7) M, a higher dose than needed for PGF2 alpha release.
In plain wordsIn lab tests on sheep womb tissue, oxytocin raised phospholipase C activity only at a higher dose than for prostaglandin release.
Peptide
Oxytocin
Outcome
IncreasePLC activity dose-response to oxytocin
Study
sheepn: not reported in source
ovine caruncular endometrial explants
Outcome group
Receptor activity and mechanism · Reproductive and sexual health
Studied dose
10(-9) to 10(-6) M · in vitro (explant incubation)As given in this study (sheep).
Source sentences
Summary
In experiment 3, explants were incubated in the presence of oxytocin or arginine vasopressin at 10(-9) to 10(-6) M to establish dose-response curves for the activation of PLC and release of PGF2 alpha. For both hormones, significant increases (P < 0.05) in the release of PGF2 alpha were observed at…
1 context sentence(design, population, supporting numbers)
Summary · context
Caruncular endometrial tissue was collected from ovariectomized ewes after completion of an 11-day steroid replacement protocol.
Report a problemFND-0807164706
07LabEx vivo tissueMechanism
In ovine endometrial explants, oxytocin stimulated PLC activity, as did AlF4-.
In plain wordsIn lab tests on sheep womb tissue, oxytocin raised phospholipase C activity.
Peptide
Oxytocin
Outcome
IncreasePLC activity after oxytocin (experiment 4)
Study
sheepn: not reported in source
ovine caruncular endometrial explants
Outcome group
Receptor activity and mechanism · Reproductive and sexual health
Studied dose
Dose not stated · in vitro (explant incubation)As given in this study (sheep).
Source sentences
Summary
In experiment 4, explants were pretreated with either U-73122 (an inhibitor of PLC activity) or U-73343 (an inactive analogue of U-73122). Explants were then treated with control medium, oxytocin or AlF4-. Both oxytocin and AlF4-stimulated the activity of PLC and the release of PGF2 alpha (P < 0.05).
1 context sentence(design, population, supporting numbers)
Summary · context
Caruncular endometrial tissue was collected from ovariectomized ewes after completion of an 11-day steroid replacement protocol.
Report a problemFND-0807164707
08LabEx vivo tissueMechanism
In ovine endometrial explants, oxytocin stimulated the release of PGF2 alpha, as did AlF4-.
In plain wordsIn lab tests on sheep womb tissue, oxytocin raised prostaglandin F2 alpha release.
Peptide
Oxytocin
Outcome
IncreasePGF2 alpha release after oxytocin (experiment 4)
Study
sheepn: not reported in source
ovine caruncular endometrial explants
Outcome group
Receptor activity and mechanism · Reproductive and sexual health
Studied dose
Dose not stated · in vitro (explant incubation)As given in this study (sheep).
Source sentences
Summary
In experiment 4, explants were pretreated with either U-73122 (an inhibitor of PLC activity) or U-73343 (an inactive analogue of U-73122). Explants were then treated with control medium, oxytocin or AlF4-. Both oxytocin and AlF4-stimulated the activity of PLC and the release of PGF2 alpha (P < 0.05).
1 context sentence(design, population, supporting numbers)
Summary · context
Caruncular endometrial tissue was collected from ovariectomized ewes after completion of an 11-day steroid replacement protocol.
Report a problemFND-0807164708

Studies that did not test the peptide alone2

In this study, Oxytocin was a lab tool or a carrier, part of a mix, or the subject of a lab method. These results are not evidence about taking Oxytocin.

09LabNot a test of Oxytocin aloneEx vivo tissueMechanism
In ovine endometrial explants, the PLC inhibitor U-73122 blocked the oxytocin-stimulated release of PGF2 alpha.
In plain wordsIn lab tests on sheep womb tissue, a phospholipase C blocker stopped oxytocin from raising prostaglandin F2 alpha release.
Peptide
Oxytocin
Outcome
DecreaseEffect of U-73122 on oxytocin-induced PGF2 alpha release
Study
sheepn: not reported in source
ovine caruncular endometrial explants
Outcome group
Receptor activity and mechanism · Reproductive and sexual health
Source sentences
Summary
U-73122 blocked the ability of oxytocin to stimulate the release of PGF2 alpha (P 0.1).
2 context sentences(design, population, supporting numbers)
Summary · context
In experiment 4, explants were pretreated with either U-73122 (an inhibitor of PLC activity) or U-73343 (an inactive analogue of U-73122). Explants were then treated with control medium, oxytocin or AlF4-.
Summary · context
Caruncular endometrial tissue was collected from ovariectomized ewes after completion of an 11-day steroid replacement protocol.
In this study, Oxytocin was a lab tool or a carrier, part of a mix, or the subject of a lab method. This result is not evidence about taking Oxytocin.
Report a problemFND-0807164709
10LabNot a test of Oxytocin aloneEx vivo tissueMechanism
In ovine endometrial explants, U-73122 had no effect on the ability of oxytocin to stimulate PLC activity.
In plain wordsIn lab tests on sheep womb tissue, the phospholipase C blocker did not change oxytocin's rise in phospholipase C activity.
Peptide
Oxytocin
Outcome
No significant changeEffect of U-73122 on oxytocin-induced PLC activity
Study
sheepn: not reported in source
ovine caruncular endometrial explants
Outcome group
Receptor activity and mechanism · Reproductive and sexual health
Source sentences
Summary
U-73122 blocked the ability of oxytocin to stimulate the release of PGF2 alpha (P 0.1).
2 context sentences(design, population, supporting numbers)
Summary · context
In experiment 4, explants were pretreated with either U-73122 (an inhibitor of PLC activity) or U-73343 (an inactive analogue of U-73122). Explants were then treated with control medium, oxytocin or AlF4-.
Summary · context
Caruncular endometrial tissue was collected from ovariectomized ewes after completion of an 11-day steroid replacement protocol.
In this study, Oxytocin was a lab tool or a carrier, part of a mix, or the subject of a lab method. This result is not evidence about taking Oxytocin.
Report a problemFND-0807164710

AI wrote and checked these results. AI can make mistakes, so check important results against the source.

Version history

PepBank keeps every version of the data on this study. Status changes from Retraction Watch, Europe PMC and Crossref are listed with their source.

Version 2Current
11 Oct 2026, 20:03 UTC
Study results updated
Version 1
9 Oct 2026, 02:51 UTC
First added · source update from Europe PMC

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Cellular mechanisms mediating the stimulation of ovine endometrial secretion of prostaglandin F2 alpha in response to oxytocin: role of phospholipase C and diacylglycerol.

Summary

Results from this study10

Studies that did not test the peptide alone2

Version history

Subjects

Publication types

MeSH terms