Effect of growth hormone (GH)-releasing peptide (GHRP) on the release of GH from cultured anterior pituitary cells in cattle.

Soliman EB, Hashizume T, Kanematsu S

Endocrine journal1 Oct 1994Cited by 6 (Europe PMC)

PMID7889121DOI10.1507/endocrj.41.585PepBankPUB-07889121

Publisher page PubMedAsk about this study

Summary

The text of this summary is not shown here, because the article license does not allow reuse. Read it at the source (opens in a new tab)

Text rights: Publisher copyright. Results still quote short attributed sentences.

Results from this study11

7 results from studies that gave the peptide. No result from this study comes from people.

01LabIn vitro (cells)Mechanism
In bovine pituitary cells, GHRP increased GH release versus controls at the higher concentration range.
In plain wordsIn cells from cattle, GHRP increased growth hormone release versus controls at higher concentrations.
Peptide
GHRP-6
Outcome
IncreaseGH release at higher concentrations
Study
cattlen: not reported in source
cultured anterior pituitary cells
Comparator
controls
Outcome group
Growth hormone and IGF-1 · Endocrine and hormones
Studied dose
10(-11) to 10(-7) M · 2 hAs given in this study (cattle).
Source sentences
Summary
At doses from 10(-11) to 10(-7) M GHRP, the amount of GH released was significantly greater than the controls (P < 0.05 to P < 0.001).
2 context sentences(design, population, supporting numbers)
Summary · context
The effect of His-D-Trp-Ala-Trp-D-Phe-Lys-NH2 (GHRP) on growth hormone (GH) release from cultured bovine anterior pituitary (AP) cells was studied in vitro with the interactive effects of GH-releasing factor (GRF: hpGRF (1-29)-NH2) and somatostatin (SRIF).
Summary · context
After 3.5 days in culture, cells were incubated for 2 h with the peptides.
Report a problemFND-0788912101
02LabIn vitro (cells)Mechanism
In bovine pituitary cells, lower GHRP concentrations did not significantly change GH release versus controls.
In plain wordsIn cells from cattle, lower GHRP concentrations did not significantly change growth hormone release versus controls.
Peptide
GHRP-6
Outcome
No significant changeGH release at lower concentrations
Study
cattlen: not reported in source
cultured anterior pituitary cells
Comparator
controls
Outcome group
Growth hormone and IGF-1 · Endocrine and hormones
Studied dose
10(-14) to 10(-12) M · 2 hAs given in this study (cattle).
Source sentences
Summary
The amounts of GH released at lower doses of GHRP (10(-14) to 10(-12) M) were not significantly different from the controls.
2 context sentences(design, population, supporting numbers)
Summary · context
The effect of His-D-Trp-Ala-Trp-D-Phe-Lys-NH2 (GHRP) on growth hormone (GH) release from cultured bovine anterior pituitary (AP) cells was studied in vitro with the interactive effects of GH-releasing factor (GRF: hpGRF (1-29)-NH2) and somatostatin (SRIF).
Summary · context
After 3.5 days in culture, cells were incubated for 2 h with the peptides.
Report a problemFND-0788912102
03LabIn vitro (cells)Mechanism
In bovine pituitary cells, GHRP produced a GH concentration of 3.98 +/- 0.27 ng/ml.
In plain wordsIn cells from cattle, GHRP produced a growth hormone concentration of 3.98 ng/ml at 10(-11) M.
Peptide
GHRP-6
Outcome
IncreaseGH concentration
Study
cattlen: not reported in source
cultured anterior pituitary cells
Outcome group
Growth hormone and IGF-1 · Endocrine and hormones
Studied dose
10(-11) M · 2 hAs given in this study (cattle).
Measurements
GH
3.98 ng/ml
10(-11) M GHRP
Source sentences
Summary
GH concentrations after treatment with 10(-11) and 10(-7) M GHRP were 3.98 +/- 0.27 and 4.81 +/- 0.16 ng/ml, respectively.
3 context sentences(design, population, supporting numbers)
Summary · context
At doses from 10(-11) to 10(-7) M GHRP, the amount of GH released was significantly greater than the controls (P < 0.05 to P < 0.001).
Summary · context
The effect of His-D-Trp-Ala-Trp-D-Phe-Lys-NH2 (GHRP) on growth hormone (GH) release from cultured bovine anterior pituitary (AP) cells was studied in vitro with the interactive effects of GH-releasing factor (GRF: hpGRF (1-29)-NH2) and somatostatin (SRIF).
Summary · context
After 3.5 days in culture, cells were incubated for 2 h with the peptides.
Report a problemFND-0788912103
04LabIn vitro (cells)Mechanism
In bovine pituitary cells, GHRP produced a GH concentration of 4.81 +/- 0.16 ng/ml.
In plain wordsIn cells from cattle, GHRP produced a growth hormone concentration of 4.81 ng/ml at 10(-7) M.
Peptide
GHRP-6
Outcome
IncreaseGH concentration
Study
cattlen: not reported in source
cultured anterior pituitary cells
Outcome group
Growth hormone and IGF-1 · Endocrine and hormones
Studied dose
10(-7) M · 2 hAs given in this study (cattle).
Measurements
GH
4.81 ng/ml
10(-7) M GHRP
Source sentences
Summary
GH concentrations after treatment with 10(-11) and 10(-7) M GHRP were 3.98 +/- 0.27 and 4.81 +/- 0.16 ng/ml, respectively.
3 context sentences(design, population, supporting numbers)
Summary · context
At doses from 10(-11) to 10(-7) M GHRP, the amount of GH released was significantly greater than the controls (P < 0.05 to P < 0.001).
Summary · context
The effect of His-D-Trp-Ala-Trp-D-Phe-Lys-NH2 (GHRP) on growth hormone (GH) release from cultured bovine anterior pituitary (AP) cells was studied in vitro with the interactive effects of GH-releasing factor (GRF: hpGRF (1-29)-NH2) and somatostatin (SRIF).
Summary · context
After 3.5 days in culture, cells were incubated for 2 h with the peptides.
Report a problemFND-0788912104
05LabIn vitro (cells)Mechanism
In bovine pituitary cells, GHRP increased GH by 57%.
In plain wordsIn cells from cattle, GHRP increased growth hormone release.
Peptide
GHRP-6
Outcome
IncreaseGH increase
Study
cattlen: not reported in source
cultured anterior pituitary cells
Outcome group
Growth hormone and IGF-1 · Endocrine and hormones
Studied dose
10(-7) M · 2 hAs given in this study (cattle).
Source sentences
Summary
In experiments performed similarly, the 10(-7) M GHRH, GHRP, and combined treatment with GHRP plus GHRH increased GH 126, 57, and 139%, respectively (P < 0.001).
2 context sentences(design, population, supporting numbers)
Summary · context
The effect of His-D-Trp-Ala-Trp-D-Phe-Lys-NH2 (GHRP) on growth hormone (GH) release from cultured bovine anterior pituitary (AP) cells was studied in vitro with the interactive effects of GH-releasing factor (GRF: hpGRF (1-29)-NH2) and somatostatin (SRIF).
Summary · context
After 3.5 days in culture, cells were incubated for 2 h with the peptides.
Report a problemFND-0788912105
06LabIn vitro (cells)Mechanism
In bovine pituitary cells, GHRP alone stimulated less GH release than GHRH alone.
In plain wordsIn cells from cattle, GHRP released less growth hormone than GHRH alone.
Peptide
GHRP-6
Outcome
DecreaseGH release versus GHRH
Study
cattlen: not reported in source
cultured anterior pituitary cells
Comparator
GHRH alone
Outcome group
Growth hormone and IGF-1 · Endocrine and hormones
Studied dose
Dose not stated · 2 hAs given in this study (cattle).
Source sentences
Summary
The GH releasing effects of either GHRH alone or GHRP plus GHRH were significantly more potent than that of GHRP alone (P < 0.001).
2 context sentences(design, population, supporting numbers)
Summary · context
The effect of His-D-Trp-Ala-Trp-D-Phe-Lys-NH2 (GHRP) on growth hormone (GH) release from cultured bovine anterior pituitary (AP) cells was studied in vitro with the interactive effects of GH-releasing factor (GRF: hpGRF (1-29)-NH2) and somatostatin (SRIF).
Summary · context
After 3.5 days in culture, cells were incubated for 2 h with the peptides.
Report a problemFND-0788912106
07LabIn vitro (cells)Mechanism
In bovine pituitary cells, adding GHRP to GHRH did not significantly increase GH release versus GHRH alone.
In plain wordsIn cells from cattle, adding GHRP to GHRH did not significantly increase growth hormone release versus GHRH alone.
Peptide
GHRP-6
Outcome
No significant changeAddition to GHRH
Study
cattlen: not reported in source
cultured anterior pituitary cells
Comparator
GHRH alone
Outcome group
Growth hormone and IGF-1 · Endocrine and hormones
Studied dose
Dose not stated · 2 hAs given in this study (cattle).
Source sentences
Summary
The additive effect was not significant when compared with GHRH alone.
3 context sentences(design, population, supporting numbers)
Summary · context
In experiments performed similarly, the 10(-7) M GHRH, GHRP, and combined treatment with GHRP plus GHRH increased GH 126, 57, and 139%, respectively (P < 0.001).
Summary · context
The effect of His-D-Trp-Ala-Trp-D-Phe-Lys-NH2 (GHRP) on growth hormone (GH) release from cultured bovine anterior pituitary (AP) cells was studied in vitro with the interactive effects of GH-releasing factor (GRF: hpGRF (1-29)-NH2) and somatostatin (SRIF).
Summary · context
After 3.5 days in culture, cells were incubated for 2 h with the peptides.
Report a problemFND-0788912109

Studies that did not test the peptide alone4

In this study, GHRP-6 was a lab tool or a carrier, part of a mix, or the subject of a lab method. These results are not evidence about taking GHRP-6.

08LabNot a test of GHRP-6 aloneIn vitro (cells)Mechanism
In bovine pituitary cells, combined GHRP and GHRH increased GH by 139%.
In plain wordsIn cells from cattle, combined GHRP and GHRH increased growth hormone release.
Peptide
GHRP-6
Outcome
IncreaseCombined GH increase
Study
cattlen: not reported in source
cultured anterior pituitary cells
Outcome group
Growth hormone and IGF-1 · Endocrine and hormones
Source sentences
Summary
In experiments performed similarly, the 10(-7) M GHRH, GHRP, and combined treatment with GHRP plus GHRH increased GH 126, 57, and 139%, respectively (P < 0.001).
2 context sentences(design, population, supporting numbers)
Summary · context
The effect of His-D-Trp-Ala-Trp-D-Phe-Lys-NH2 (GHRP) on growth hormone (GH) release from cultured bovine anterior pituitary (AP) cells was studied in vitro with the interactive effects of GH-releasing factor (GRF: hpGRF (1-29)-NH2) and somatostatin (SRIF).
Summary · context
After 3.5 days in culture, cells were incubated for 2 h with the peptides.
In this study, GHRP-6 was a lab tool or a carrier, part of a mix, or the subject of a lab method. This result is not evidence about taking GHRP-6.
Report a problemFND-0788912107
09LabNot a test of GHRP-6 aloneIn vitro (cells)Mechanism
In bovine pituitary cells, combined GHRP and GHRH released more GH than GHRP alone.
In plain wordsIn cells from cattle, combined GHRP and GHRH released more growth hormone than GHRP alone.
Peptide
GHRP-6
Outcome
IncreaseCombination versus GHRP
Study
cattlen: not reported in source
cultured anterior pituitary cells
Comparator
GHRP alone
Outcome group
Growth hormone and IGF-1 · Endocrine and hormones
Source sentences
Summary
The GH releasing effects of either GHRH alone or GHRP plus GHRH were significantly more potent than that of GHRP alone (P < 0.001).
2 context sentences(design, population, supporting numbers)
Summary · context
The effect of His-D-Trp-Ala-Trp-D-Phe-Lys-NH2 (GHRP) on growth hormone (GH) release from cultured bovine anterior pituitary (AP) cells was studied in vitro with the interactive effects of GH-releasing factor (GRF: hpGRF (1-29)-NH2) and somatostatin (SRIF).
Summary · context
After 3.5 days in culture, cells were incubated for 2 h with the peptides.
In this study, GHRP-6 was a lab tool or a carrier, part of a mix, or the subject of a lab method. This result is not evidence about taking GHRP-6.
Report a problemFND-0788912108
10LabNot a test of GHRP-6 aloneIn vitro (cells)Mechanism
In bovine pituitary cells, SRIF inhibited GHRP-induced GH release versus untreated controls.
In plain wordsIn cells from cattle, SRIF reduced GHRP-induced growth hormone release versus untreated controls.
Peptide
GHRP-6
Outcome
DecreaseSRIF inhibition of GH release
Study
cattlen: not reported in source
cultured anterior pituitary cells
Comparator
untreated control
Outcome group
Growth hormone and IGF-1 · Endocrine and hormones
Source sentences
Summary
GH release induced by either GHRH or GHRP was significantly inhibited by SRIF (P < 0.01) compared with the untreated control.
2 context sentences(design, population, supporting numbers)
Summary · context
The effect of His-D-Trp-Ala-Trp-D-Phe-Lys-NH2 (GHRP) on growth hormone (GH) release from cultured bovine anterior pituitary (AP) cells was studied in vitro with the interactive effects of GH-releasing factor (GRF: hpGRF (1-29)-NH2) and somatostatin (SRIF).
Summary · context
After 3.5 days in culture, cells were incubated for 2 h with the peptides.
In this study, GHRP-6 was a lab tool or a carrier, part of a mix, or the subject of a lab method. This result is not evidence about taking GHRP-6.
Report a problemFND-0788912110
11LabNot a test of GHRP-6 aloneIn vitro (cells)Mechanism
In bovine pituitary cells, SRIF inhibited GH release less with combined GHRP and GHRH than with either peptide alone.
In plain wordsIn cells from cattle, SRIF reduced growth hormone release less with combined GHRP and GHRH than with either peptide alone.
Peptide
GHRP-6
Outcome
DecreaseSRIF inhibition in combination
Study
cattlen: not reported in source
cultured anterior pituitary cells
Comparator
SRIF with GHRH or GHRP alone
Outcome group
Growth hormone and IGF-1 · Endocrine and hormones
Source sentences
Summary
The inhibitory effect of SRIF in combined treatment with GHRP plus GHRH was significantly less than that of SRIF with GHRH or with GHRP (P < 0.01).
2 context sentences(design, population, supporting numbers)
Summary · context
The effect of His-D-Trp-Ala-Trp-D-Phe-Lys-NH2 (GHRP) on growth hormone (GH) release from cultured bovine anterior pituitary (AP) cells was studied in vitro with the interactive effects of GH-releasing factor (GRF: hpGRF (1-29)-NH2) and somatostatin (SRIF).
Summary · context
After 3.5 days in culture, cells were incubated for 2 h with the peptides.
In this study, GHRP-6 was a lab tool or a carrier, part of a mix, or the subject of a lab method. This result is not evidence about taking GHRP-6.
Report a problemFND-0788912111

AI wrote and checked these results. AI can make mistakes, so check important results against the source.

Version history

PepBank keeps every version of the data on this study. Status changes from Retraction Watch, Europe PMC and Crossref are listed with their source.

Version 2Current
21 Oct 2026, 15:58 UTC
Study results updated
Version 1
9 Oct 2026, 03:13 UTC
First added · source update from Europe PMC

Subjects

Terms as the source lists them.

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