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Study · In vitro (cells) · bovine · 1995

3 results

Peptidyl dipeptidase A-catalyzed metabolism of delta sleep-inducing peptide in bovine brain microvessel endothelial cells: a cell culture model of the blood brain barrier.

Augustijns PF, Ng KY, Williams TM, Borchardt RT

Biochemical and biophysical research communications1 May 1995Cited by 4 (Europe PMC)

PMID7763273 (opens in a new tab)DOI10.1006/bbrc.1995.1754 (opens in a new tab)PepBankPUB-07763273
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Summary

The text of this summary is not shown here, because the article license does not allow reuse. Read it at the source (opens in a new tab)

Text rights: Publisher copyright. Results still quote short attributed sentences.

Results from this study3

1 result from studies that gave the peptide. No result from this study comes from people.

  1. 01LabIn vitro (cells)Pharmacokinetics

    In cultured bovine brain microvessel endothelial cells, peptidyl dipeptidase A metabolized DSIP by sequential hydrolysis of dipeptides or tripeptides from the carboxyl terminus.

    In plain wordsIn cells modelling the blood-brain barrier, an enzyme broke down DSIP by cutting two or three amino acids at a time from its end.

    Peptide
    Delta sleep-inducing peptide (DSIP)
    Outcome
    Not applicableMetabolism of DSIP by peptidyl dipeptidase A in BBMEC
    Study

    bovinen: not reported in source

    Cultured bovine brain microvessel endothelial cells (BBMEC), blood-brain barrier model
    Outcome group
    Pharmacokinetics · Pharmacology
    Studied dose
    Dose not stated

    Source sentences

    Summary
    peptidyl dipeptidase A in cultured bovine brain microvessel endothelial cells (BBMEC), a model of the BBB, and a purified form of this enzyme can also metabolize DSIP by sequential hydrolyses of dipeptides or tripeptides from the carboxyl terminus of this nonapeptide
    1 context sentence
    Summary · context
    In this study, we have shown that peptidyl dipeptidase A in cultured bovine brain microvessel endothelial cells (BBMEC), a model of the BBB, and a purified form of this enzyme can also metabolize DSIP by sequential hydrolyses of dipeptides or tripeptides from the carboxyl terminus of this nonapeptide.

Studies that did not test the peptide alone2

In this study, Delta sleep-inducing peptide (DSIP) was a lab tool or a carrier, part of a mix, or the subject of a lab method. These results are not evidence about taking Delta sleep-inducing peptide (DSIP).

  1. 02LabNot a test of Delta sleep-inducing peptide (DSIP) aloneIn vitro (cells)Activity measurement

    Captopril inhibited both the dipeptidase and tripeptidase activity of peptidyl dipeptidase A on DSIP.

    In plain wordsIn lab tests, captopril blocked the enzyme activity that breaks down DSIP.

    Peptide
    Delta sleep-inducing peptide (DSIP)
    Outcome
    DecreaseCaptopril inhibition of peptidyl dipeptidase A dipeptidase and tripeptidase activity
    Study

    bovinen: not reported in source

    Cultured bovine brain microvessel endothelial cells (BBMEC), blood-brain barrier model
    Outcome group
    Receptor activity and mechanism · Pharmacology

    Source sentences

    Summary
    Both the dipeptidase and tripeptidase activity associated with peptidyl dipeptidase A can be inhibited by captopril.
    1 context sentence
    Summary · context
    In this study, we have shown that peptidyl dipeptidase A in cultured bovine brain microvessel endothelial cells (BBMEC), a model of the BBB, and a purified form of this enzyme can also metabolize DSIP by sequential hydrolyses of dipeptides or tripeptides from the carboxyl terminus of this nonapeptide.

    In this study, Delta sleep-inducing peptide (DSIP) was a lab tool or a carrier, part of a mix, or the subject of a lab method. This result is not evidence about taking Delta sleep-inducing peptide (DSIP).

  2. 03LabNot a test of Delta sleep-inducing peptide (DSIP) aloneIn vitro (cells)Pharmacokinetics

    Inclusion of captopril and bestatin achieved total stabilization of DSIP to metabolism in BBMEC.

    In plain wordsIn cells modelling the blood-brain barrier, adding two enzyme blockers fully protected DSIP from being broken down.

    Peptide
    Delta sleep-inducing peptide (DSIP)
    Outcome
    IncreaseStabilization of DSIP with captopril and bestatin in BBMEC
    Study

    bovinen: not reported in source

    Cultured bovine brain microvessel endothelial cells (BBMEC), blood-brain barrier model
    Outcome group
    Pharmacokinetics · Pharmacology

    Source sentences

    Summary
    Total stabilization of DSIP to metabolism in BBMEC could be achieved by inclusion of an inhibitor of peptidyl dipeptidase A (e.g., captopril) and an inhibitor of aminopeptidases (e.g., bestatin).
    1 context sentence
    Summary · context
    In this study, we have shown that peptidyl dipeptidase A in cultured bovine brain microvessel endothelial cells (BBMEC), a model of the BBB, and a purified form of this enzyme can also metabolize DSIP by sequential hydrolyses of dipeptides or tripeptides from the carboxyl terminus of this nonapeptide.

    In this study, Delta sleep-inducing peptide (DSIP) was a lab tool or a carrier, part of a mix, or the subject of a lab method. This result is not evidence about taking Delta sleep-inducing peptide (DSIP).

AI wrote and checked these results. AI can make mistakes, so check important results against the source.

Version history

PepBank keeps every version of the data on this study. Status changes from Retraction Watch, Europe PMC and Crossref are listed with their source.

  1. Version 2Current

    10 Oct 2026, 08:55 UTC

    Study results updated

  2. Version 1

    9 Oct 2026, 03:04 UTC

    First added · source update from Europe PMC

Subjects

Terms as the source lists them.

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