Skip to content

Study · Not a test of the peptide alone · 1995

1 result

Cloning, expression, and purification of rat brain protein L-isoaspartyl methyltransferase.

David CL, Aswad DW

Protein expression and purification1 Jun 1995Cited by 16 (Europe PMC)

PMID7663167 (opens in a new tab)DOI10.1006/prep.1995.1041 (opens in a new tab)PepBankPUB-07663167
Publisher pagePubMedAsk about this study
Jump to 1 result

Summary

The text of this summary is not shown here, because the article license does not allow reuse. Read it at the source (opens in a new tab)

Text rights: Publisher copyright. Results still quote short attributed sentences.

Results from this study1

No result from this study comes from a test of the peptide.

Studies that did not test the peptide alone1

In this study, Delta sleep-inducing peptide (DSIP) was a lab tool or a carrier, part of a mix, or the subject of a lab method. These results are not evidence about taking Delta sleep-inducing peptide (DSIP).

  1. 01LabNot a test of Delta sleep-inducing peptide (DSIP) aloneIn vitro (cell-free)Activity measurement

    With isoaspartyl DSIP as methyl acceptor, recombinant rat brain PIMT showed normal Michaelis-Menten kinetics with Km for the peptide of 16 microM.

    In plain wordsIn lab tests, an enzyme methylated an altered form of DSIP with normal enzyme kinetics.

    Peptide
    Delta sleep-inducing peptide (DSIP)
    Outcome
    Not applicableMichaelis-Menten kinetics of rrPIMT with isoaspartyl DSIP as methyl acceptor
    Study

    n: not reported in source

    Recombinant rat brain PIMT enzyme
    Outcome group
    Receptor activity and mechanism · Pharmacology

    Measurements

    Km (S-adenosyl-L-methionine)
    1.1 microM
    isoaspartyl DSIP as methyl acceptor
    Km (peptide)
    16 microM
    isoaspartyl DSIP as methyl acceptor

    Source sentences

    Summary
    Using isoaspartyl delta sleep-inducing peptide as the methyl acceptor, rrPIMT exhibited normal Michaelis-Menten kinetics that yielded the following constants: Km (S-adenosyl-L-methionine) = 1.1 microM, Km (peptide) = 16 microM, Vmax = 60,000 pmol/min/mg.

    In this study, Delta sleep-inducing peptide (DSIP) was a lab tool or a carrier, part of a mix, or the subject of a lab method. This result is not evidence about taking Delta sleep-inducing peptide (DSIP).

AI wrote and checked these results. AI can make mistakes, so check important results against the source.

Version history

PepBank keeps every version of the data on this study. Status changes from Retraction Watch, Europe PMC and Crossref are listed with their source.

  1. Version 2Current

    10 Oct 2026, 08:55 UTC

    Study results updated

  2. Version 1

    9 Oct 2026, 03:04 UTC

    First added · source update from Europe PMC

Subjects

Terms as the source lists them.

PepBank is a research reference. Talk to a doctor before you use any peptide.