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Study · In vitro (cells) · sheep · 1995

7 results

Effects of growth hormone-releasing peptide-2 (GHRP-2) on membrane Ca2+ permeability in cultured ovine somatotrophs.

Chen C, Clarke IJ

Journal of neuroendocrinology1 Mar 1995Cited by 21 (Europe PMC)

PMID7606243 (opens in a new tab)DOI10.1111/j.1365-2826.1995.tb00745.x (opens in a new tab)PepBankPUB-07606243
Publisher pagePubMedAsk about this study
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Summary

The text of this summary is not shown here, because the article license does not allow reuse. Read it at the source (opens in a new tab)

Text rights: Publisher copyright. Results still quote short attributed sentences.

Results from this study7

7 results from studies that gave the peptide. No result from this study comes from people.

  1. 01LabIn vitro (cells)Mechanism

    In cultured ovine somatotrophs, GHRP‑2 (10 nM) depolarized the membrane potential and triggered a burst of action potentials.

    In plain wordsIn cultured sheep pituitary cells, GHRP‑2 depolarized the cell membrane and triggered a burst of electrical signals.

    Peptide
    GHRP-2
    Outcome
    IncreaseMembrane potential and action potentials
    Study

    sheepn: not reported in source

    Primary culture of ovine somatotroph-enriched pituitary cells
    Outcome group
    Receptor activity and mechanism · Endocrine and hormones
    Studied dose
    10 nMAs given in this study (sheep).

    Source sentences

    Summary
    Using a standard bath solution (containing Na+, Ca2+) and an electrode solution containing K+ in current-clamp recordings, GHRP-2 (10 nM) depolarized the membrane potential of the cells triggering a burst of action potentials.
    2 context sentences
    Summary · context
    The newly synthesized GH-releasing peptide, GHRP-2 (D-Ala-D-beta Nal-Ala-Trp-D-Phe-Lys-NH2), was studied in somatotroph-enriched populations of ovine pituitary cells in primary culture.
    Summary · context
    Nystatin-perforated whole-cell recordings were made on identified somatotrophs after 4-14 days of culture.
  2. 02LabIn vitro (cells)Mechanism

    In cultured ovine somatotrophs, GHRP‑2 produced a slowly inactivated inward current with a slight reduction in outward current.

    In plain wordsIn cultured sheep pituitary cells, GHRP‑2 produced an inward electrical current and slightly reduced the outward current.

    Peptide
    GHRP-2
    Outcome
    IncreaseInward current
    Study

    sheepn: not reported in source

    Primary culture of ovine somatotroph-enriched pituitary cells
    Outcome group
    Receptor activity and mechanism · Endocrine and hormones
    Studied dose
    Dose not stated

    Source sentences

    Summary
    Voltage-clamp recordings indicated that GHRP-2 produced a slowly inactivated inward current with a slight reduction in outward current.
    2 context sentences
    Summary · context
    The newly synthesized GH-releasing peptide, GHRP-2 (D-Ala-D-beta Nal-Ala-Trp-D-Phe-Lys-NH2), was studied in somatotroph-enriched populations of ovine pituitary cells in primary culture.
    Summary · context
    Nystatin-perforated whole-cell recordings were made on identified somatotrophs after 4-14 days of culture.
  3. 03LabIn vitro (cells)Mechanism

    In cultured ovine somatotrophs, the GHRP‑2-induced inward current was blocked by the Ca2+ channel blocker Co2+ (0.5 mM).

    In plain wordsIn cultured sheep pituitary cells, the electrical current caused by GHRP‑2 was blocked by a calcium channel blocker.

    Peptide
    GHRP-2
    Outcome
    Not applicableGHRP-2-induced inward current blocked by Co2+
    Study

    sheepn: not reported in source

    Primary culture of ovine somatotroph-enriched pituitary cells
    Comparator
    Co2+ 0.5 mM
    Outcome group
    Receptor activity and mechanism · Endocrine and hormones
    Studied dose
    Dose not stated

    Source sentences

    Summary
    The inward current was blocked by the Ca2+ channel blocker, Co2+ (0.5 mM).
    2 context sentences
    Summary · context
    The newly synthesized GH-releasing peptide, GHRP-2 (D-Ala-D-beta Nal-Ala-Trp-D-Phe-Lys-NH2), was studied in somatotroph-enriched populations of ovine pituitary cells in primary culture.
    Summary · context
    Nystatin-perforated whole-cell recordings were made on identified somatotrophs after 4-14 days of culture.
  4. 04LabIn vitro (cells)Mechanism

    In cultured ovine somatotrophs, GHRP‑2 increased both T type and L type Ca2+ currents.

    In plain wordsIn cultured sheep pituitary cells, GHRP‑2 increased both types of calcium current.

    Peptide
    GHRP-2
    Outcome
    IncreaseT type and L type Ca2+ currents
    Study

    sheepn: not reported in source

    Primary culture of ovine somatotroph-enriched pituitary cells
    Outcome group
    Receptor activity and mechanism · Endocrine and hormones
    Studied dose
    Dose not stated

    Source sentences

    Summary
    Current-voltage relationships indicated an increase in both T and L type Ca2+ currents in response to GHRP-2.
    2 context sentences
    Summary · context
    The newly synthesized GH-releasing peptide, GHRP-2 (D-Ala-D-beta Nal-Ala-Trp-D-Phe-Lys-NH2), was studied in somatotroph-enriched populations of ovine pituitary cells in primary culture.
    Summary · context
    Nystatin-perforated whole-cell recordings were made on identified somatotrophs after 4-14 days of culture.
  5. 05LabIn vitro (cells)Mechanism

    In cultured ovine somatotrophs, GHRP‑2 shifted the voltage-dependent inactivation curve for T type Ca2+ current towards a less negative level.

    In plain wordsIn cultured sheep pituitary cells, GHRP‑2 shifted the voltage at which one type of calcium current switches off.

    Peptide
    GHRP-2
    Outcome
    Not applicableVoltage-dependent inactivation of T type Ca2+ current
    Study

    sheepn: not reported in source

    Primary culture of ovine somatotroph-enriched pituitary cells
    Outcome group
    Receptor activity and mechanism · Endocrine and hormones
    Studied dose
    Dose not stated

    Source sentences

    Summary
    The voltage-dependent inactivation curve for T type Ca2+ current was shifted towards a less negative level by the peptide.
    2 context sentences
    Summary · context
    The newly synthesized GH-releasing peptide, GHRP-2 (D-Ala-D-beta Nal-Ala-Trp-D-Phe-Lys-NH2), was studied in somatotroph-enriched populations of ovine pituitary cells in primary culture.
    Summary · context
    Nystatin-perforated whole-cell recordings were made on identified somatotrophs after 4-14 days of culture.
  6. 06LabIn vitro (cells)Mechanism

    In ovine somatotroph-enriched cell populations, GHRP‑2 specifically increased intracellular free Ca2+ concentration.

    In plain wordsIn sheep pituitary cell cultures, GHRP‑2 raised the calcium level inside the cells.

    Peptide
    GHRP-2
    Outcome
    IncreaseIntracellular free Ca2+ concentration
    Study

    sheepn: not reported in source

    Primary culture of ovine somatotroph-enriched pituitary cells
    Outcome group
    Receptor activity and mechanism · Endocrine and hormones
    Studied dose
    Dose not stated

    Source sentences

    Summary
    Intracellular free Ca2+ concentration ([Ca2+]i) in somatotroph-enriched populations was specifically increased by GHRP-2
    2 context sentences
    Summary · context
    The newly synthesized GH-releasing peptide, GHRP-2 (D-Ala-D-beta Nal-Ala-Trp-D-Phe-Lys-NH2), was studied in somatotroph-enriched populations of ovine pituitary cells in primary culture.
    Summary · context
    Nystatin-perforated whole-cell recordings were made on identified somatotrophs after 4-14 days of culture.
  7. 07LabIn vitro (cells)Mechanism

    In ovine somatotroph-enriched cell populations, blockade of membrane Ca2+ channels totally abolished the GHRP‑2-induced rise in intracellular Ca2+.

    In plain wordsIn sheep pituitary cell cultures, blocking calcium channels completely stopped the calcium rise caused by GHRP‑2.

    Peptide
    GHRP-2
    Outcome
    Not applicableGHRP-2-induced rise in intracellular Ca2+ after Ca2+ channel blockade
    Study

    sheepn: not reported in source

    Primary culture of ovine somatotroph-enriched pituitary cells
    Comparator
    Blockade of membrane Ca2+ channels
    Outcome group
    Receptor activity and mechanism · Endocrine and hormones
    Studied dose
    Dose not stated

    Source sentences

    Summary
    but this effect was totally abolished by blockade of membrane Ca2+ channels.
    2 context sentences
    Summary · context
    The newly synthesized GH-releasing peptide, GHRP-2 (D-Ala-D-beta Nal-Ala-Trp-D-Phe-Lys-NH2), was studied in somatotroph-enriched populations of ovine pituitary cells in primary culture.
    Summary · context
    Nystatin-perforated whole-cell recordings were made on identified somatotrophs after 4-14 days of culture.

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Version history

PepBank keeps every version of the data on this study. Status changes from Retraction Watch, Europe PMC and Crossref are listed with their source.

  1. Version 2Current

    13 Oct 2026, 21:44 UTC

    Study results updated

  2. Version 1

    13 Oct 2026, 16:31 UTC

    First added · source update from Europe PMC

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