Differences in the association of insulin-like growth factor-I (IGF-I) and IGF-I variants with rat, sheep, pig, human and chicken plasma-binding proteins.

Lord AP, Bastian SE, Read LC, Walton PE, Ballard FJ

The Journal of endocrinology1 Mar 1994Cited by 12 (Europe PMC)

PMID7514204DOI10.1677/joe.0.1400475PepBankPUB-07514204

Publisher page PubMedAsk about this study

Summary

The text of this summary is not shown here, because the article license does not allow reuse. Read it at the source (opens in a new tab)

Text rights: Publisher copyright. Results still quote short attributed sentences.

Results from this study8

3 results from studies that gave the peptide. No result from this study comes from people.

01LabEx vivo tissueActivity measurement
IGF-I variants including LR3IGF-I bound very weakly in rat plasma but bound significantly in sheep, human, pig and chicken plasma.
In plain wordsIn lab tests, the LR3 IGF-I variant stuck to plasma proteins from sheep, human, pig and chicken, but hardly at all to rat plasma.
Peptide
IGF-1 LR3
Outcome
Not applicableBinding of IGF-I variants in plasma across species
Study
rat, sheep, human, pig, chickenn: not reported in source
plasma and purified IGF-binding proteins
Comparator
rat plasma
Outcome group
Receptor activity and mechanism · Pharmacology
Studied dose
Dose not stated
Source sentences
Summary
Marked species differences were demonstrated, notably that the IGF-I variants which exhibited extremely weak binding in rat plasma bound significantly in plasma from the other species.
3 context sentences(design, population, supporting numbers)
Summary · context
This result was shown both by size-exclusion chromatography of labelled IGFs added to plasma, in which the extent of variant IGF-I binding decreased in the order sheep > human > pig = chicken > rat, and by competition for labelled IGF-I binding in vitro, in which the order was pig = chicken > sheep > human > rat.
Summary · context
Associations between labelled insulin-like growth factors (IGFs) and IGF-binding proteins in plasma have been compared in the rat, sheep, human, pig and chicken.
Summary · context
The IGFs tested were recombinant human IGF-I, the truncated variant, des(1-3)IGF-I, and LR3IGF-I, an extended form that had been engineered so as to minimize interactions with IGF-binding proteins.
Report a problemFND-0751420401
02LabEx vivo tissueActivity measurement
With purified rat, sheep and human IGF-binding protein-3, the two IGF-I variants including LR3IGF-I showed only slight differences between species.
In plain wordsIn lab tests, the LR3 IGF-I variant bound purified binding protein-3 from rat, sheep and human with only slight differences.
Peptide
IGF-1 LR3
Outcome
Not applicableBinding to purified IGF-binding protein-3 across species
Study
rat, sheep, human, pig, chickenn: not reported in source
plasma and purified IGF-binding proteins
Outcome group
Receptor activity and mechanism · Pharmacology
Studied dose
Dose not stated
Source sentences
Summary
Notwithstanding these differences, the two IGF-I variants showed only slight between-species binding differences when tested with purified rat, sheep and human IGF-binding protein-3.
2 context sentences(design, population, supporting numbers)
Summary · context
Associations between labelled insulin-like growth factors (IGFs) and IGF-binding proteins in plasma have been compared in the rat, sheep, human, pig and chicken.
Summary · context
The IGFs tested were recombinant human IGF-I, the truncated variant, des(1-3)IGF-I, and LR3IGF-I, an extended form that had been engineered so as to minimize interactions with IGF-binding proteins.
Report a problemFND-0751420402
03LabEx vivo tissueActivity measurement
In ligand blotting of plasma from five species, LR3IGF-I bound less than des(1-3)IGF-I, which bound less than IGF-I.
In plain wordsIn lab tests with plasma from five species, the LR3 IGF-I variant bound binding proteins less than IGF-I or the des(1-3) variant.
Peptide
IGF-1 LR3
Outcome
DecreaseLigand-blotting binding of LR3IGF-I versus IGF-I and des(1-3)IGF-I
Study
rat, sheep, human, pig, chickenn: not reported in source
plasma and purified IGF-binding proteins
Comparator
IGF-I and des(1-3)IGF-I
Outcome group
Receptor activity and mechanism · Pharmacology
Studied dose
Dose not stated
Source sentences
Summary
Ligand blotting experiments with plasma from the five species similarly showed a consistent pattern in that IGF-I binding was much greater than des(1-3)IGF-I binding, which in turn was greater than LR3IGF-I binding.(ABSTRACT TRUNCATED AT 250 WORDS)
2 context sentences(design, population, supporting numbers)
Summary · context
Associations between labelled insulin-like growth factors (IGFs) and IGF-binding proteins in plasma have been compared in the rat, sheep, human, pig and chicken.
Summary · context
The IGFs tested were recombinant human IGF-I, the truncated variant, des(1-3)IGF-I, and LR3IGF-I, an extended form that had been engineered so as to minimize interactions with IGF-binding proteins.
Report a problemFND-0751420403

Studies that did not test the peptide alone5

In this study, IGF-1 DES (des(1-3)IGF-I) was a lab tool or a carrier, part of a mix, or the subject of a lab method. These results are not evidence about taking IGF-1 DES (des(1-3)IGF-I).

04LabNot a test of IGF-1 DES (des(1-3)IGF-I) aloneIn vitro (cell-free)Activity measurement
In plasma assays, IGF variants including des(1‑3)IGF-I showed binding in the order sheep, human, pig and chicken equally, then rat.
In plain wordsIn lab tests, pooled IGF variants bound most in sheep plasma and least in rat plasma.
Peptide
IGF-1 DES (des(1-3)IGF-I)
Outcome
MixedPlasma binding by size-exclusion chromatography
Study
rat, sheep, pig, human and chickenn: not reported in source
Plasma and purified IGFBP-3 binding assays
Comparator
Plasma from different species
Outcome group
Receptor activity and mechanism · Pharmacology
Source sentences
Summary
This result was shown both by size-exclusion chromatography of labelled IGFs added to plasma, in which the extent of variant IGF-I binding decreased in the order sheep > human > pig = chicken > rat
2 context sentences(design, population, supporting numbers)
Summary · context
Associations between labelled insulin-like growth factors (IGFs) and IGF-binding proteins in plasma have been compared in the rat, sheep, human, pig and chicken.
Summary · context
The IGFs tested were recombinant human IGF-I, the truncated variant, des(1-3)IGF-I, and LR3IGF-I, an extended form that had been engineered so as to minimize interactions with IGF-binding proteins.
In this study, IGF-1 DES (des(1-3)IGF-I) was a lab tool or a carrier, part of a mix, or the subject of a lab method. This result is not evidence about taking IGF-1 DES (des(1-3)IGF-I).
Report a problemFND-0751420404
05LabNot a test of IGF-1 DES (des(1-3)IGF-I) aloneIn vitro (cell-free)Activity measurement
In competition assays, IGF variants including des(1‑3)IGF-I showed binding in the order pig and chicken equally, sheep, human, then rat.
In plain wordsIn lab tests, pooled IGF variants competed most strongly in pig and chicken plasma and least strongly in rat plasma.
Peptide
IGF-1 DES (des(1-3)IGF-I)
Outcome
MixedCompetitive plasma binding
Study
rat, sheep, pig, human and chickenn: not reported in source
Plasma and purified IGFBP-3 binding assays
Comparator
Plasma from different species
Outcome group
Receptor activity and mechanism · Pharmacology
Source sentences
Summary
and by competition for labelled IGF-I binding in vitro, in which the order was pig = chicken > sheep > human > rat.
2 context sentences(design, population, supporting numbers)
Summary · context
Associations between labelled insulin-like growth factors (IGFs) and IGF-binding proteins in plasma have been compared in the rat, sheep, human, pig and chicken.
Summary · context
The IGFs tested were recombinant human IGF-I, the truncated variant, des(1-3)IGF-I, and LR3IGF-I, an extended form that had been engineered so as to minimize interactions with IGF-binding proteins.
In this study, IGF-1 DES (des(1-3)IGF-I) was a lab tool or a carrier, part of a mix, or the subject of a lab method. This result is not evidence about taking IGF-1 DES (des(1-3)IGF-I).
Report a problemFND-0751420405
06LabNot a test of IGF-1 DES (des(1-3)IGF-I) aloneIn vitro (cell-free)Activity measurement
In purified IGFBP-3 assays, the two IGF-I variants including des(1‑3)IGF-I showed only slight binding differences across rat, sheep and human proteins.
In plain wordsIn lab tests, pooled IGF variants showed only small differences in binding to purified IGFBP-3 across the tested species.
Peptide
IGF-1 DES (des(1-3)IGF-I)
Outcome
MixedPurified IGFBP-3 binding across species
Study
rat, sheep and humann: not reported in source
Purified rat, sheep and human IGFBP-3 binding assays
Comparator
Rat, sheep and human IGFBP-3
Outcome group
Receptor activity and mechanism · Pharmacology
Source sentences
Summary
Notwithstanding these differences, the two IGF-I variants showed only slight between-species binding differences when tested with purified rat, sheep and human IGF-binding protein-3.
2 context sentences(design, population, supporting numbers)
Summary · context
Associations between labelled insulin-like growth factors (IGFs) and IGF-binding proteins in plasma have been compared in the rat, sheep, human, pig and chicken.
Summary · context
The IGFs tested were recombinant human IGF-I, the truncated variant, des(1-3)IGF-I, and LR3IGF-I, an extended form that had been engineered so as to minimize interactions with IGF-binding proteins.
In this study, IGF-1 DES (des(1-3)IGF-I) was a lab tool or a carrier, part of a mix, or the subject of a lab method. This result is not evidence about taking IGF-1 DES (des(1-3)IGF-I).
Report a problemFND-0751420406
07LabNot a test of IGF-1 DES (des(1-3)IGF-I) aloneIn vitro (cell-free)Activity measurement
In plasma ligand blots across five species, des(1‑3)IGF-I bound less than IGF-I.
In plain wordsIn lab tests, des(1‑3)IGF-I bound plasma proteins less than IGF-I.
Peptide
IGF-1 DES (des(1-3)IGF-I)
Outcome
DecreasePlasma binding versus IGF-I
Study
rat, sheep, pig, human and chickenn: not reported in source
Plasma and purified IGFBP-3 binding assays
Comparator
IGF-I
Outcome group
Receptor activity and mechanism · Pharmacology
Source sentences
Summary
Ligand blotting experiments with plasma from the five species similarly showed a consistent pattern in that IGF-I binding was much greater than des(1-3)IGF-I binding, which in turn was greater than LR3IGF-I binding.(ABSTRACT TRUNCATED AT 250 WORDS)
2 context sentences(design, population, supporting numbers)
Summary · context
Associations between labelled insulin-like growth factors (IGFs) and IGF-binding proteins in plasma have been compared in the rat, sheep, human, pig and chicken.
Summary · context
The IGFs tested were recombinant human IGF-I, the truncated variant, des(1-3)IGF-I, and LR3IGF-I, an extended form that had been engineered so as to minimize interactions with IGF-binding proteins.
In this study, IGF-1 DES (des(1-3)IGF-I) was a lab tool or a carrier, part of a mix, or the subject of a lab method. This result is not evidence about taking IGF-1 DES (des(1-3)IGF-I).
Report a problemFND-0751420407
08LabNot a test of IGF-1 DES (des(1-3)IGF-I) aloneIn vitro (cell-free)Activity measurement
In plasma ligand blots across five species, des(1‑3)IGF-I bound more than LR3IGF-I.
In plain wordsIn lab tests, des(1‑3)IGF-I bound plasma proteins more than LR3IGF-I.
Peptide
IGF-1 DES (des(1-3)IGF-I)
Outcome
IncreasePlasma binding versus LR3IGF-I
Study
rat, sheep, pig, human and chickenn: not reported in source
Plasma and purified IGFBP-3 binding assays
Comparator
LR3IGF-I
Outcome group
Receptor activity and mechanism · Pharmacology
Source sentences
Summary
Ligand blotting experiments with plasma from the five species similarly showed a consistent pattern in that IGF-I binding was much greater than des(1-3)IGF-I binding, which in turn was greater than LR3IGF-I binding.(ABSTRACT TRUNCATED AT 250 WORDS)
2 context sentences(design, population, supporting numbers)
Summary · context
Associations between labelled insulin-like growth factors (IGFs) and IGF-binding proteins in plasma have been compared in the rat, sheep, human, pig and chicken.
Summary · context
The IGFs tested were recombinant human IGF-I, the truncated variant, des(1-3)IGF-I, and LR3IGF-I, an extended form that had been engineered so as to minimize interactions with IGF-binding proteins.
In this study, IGF-1 DES (des(1-3)IGF-I) was a lab tool or a carrier, part of a mix, or the subject of a lab method. This result is not evidence about taking IGF-1 DES (des(1-3)IGF-I).
Report a problemFND-0751420408

AI wrote and checked these results. AI can make mistakes, so check important results against the source.

Version history

PepBank keeps every version of the data on this study. Status changes from Retraction Watch, Europe PMC and Crossref are listed with their source.

Versions 3 to 4Current
21 Oct 2026, 13:52 UTC
The same notice was added 2 times, first on 21 Oct 2026, 12:50 UTC. The status did not change between them.
Study data updated · source update from Europe PMC
Version 2
13 Oct 2026, 21:44 UTC
Study results updated
Version 1
13 Oct 2026, 16:31 UTC
First added · source update from Europe PMC

Differences in the association of insulin-like growth factor-I (IGF-I) and IGF-I variants with rat, sheep, pig, human and chicken plasma-binding proteins.

Summary

Results from this study8

Studies that did not test the peptide alone5

Version history

Subjects

Publication types

MeSH terms