Direct in vivo demonstration by radioautography of specific binding sites for calcitonin in skeletal and renal tissues of the rat.

Warshawsky H, Goltzman D, Rouleau MF, Bergeron JJ

The Journal of cell biology1 Jun 1980Cited by 129 (Europe PMC)

PMID7391137PMCIDPMC2111446DOI10.1083/jcb.85.3.682PepBankPUB-07391137

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Summary

The text of this summary is not shown here, because the article license does not allow reuse. Read it at the source (opens in a new tab)

Text rights: CC BY-NC. Results still quote short attributed sentences.

Results from this study4

No result from this study comes from a test of the peptide.

Studies that did not test the peptide alone4

In this study, Calcitonin-salmon was a lab tool or a carrier, part of a mix, or the subject of a lab method. These results are not evidence about taking Calcitonin-salmon.

01AnimalNot a test of Calcitonin-salmon aloneAnimal study (in vivo)Activity measurement
In rats, labeled salmon calcitonin localized mainly to vesicles below the proximal tubule brush border, persisting with excess unlabeled calcitonin.
In plain wordsIn rats, labeled salmon calcitonin remained in proximal kidney tubule vesicles despite excess unlabeled calcitonin.
Peptide
Calcitonin-salmon
Outcome
Not applicableProximal renal tubule localization
Study
ratn: not reported in source
Rat tissues after in-vivo calcitonin injection
Outcome group
Receptor activity and mechanism · Kidney and urinary
Source sentences
Summary
In kidney, most silver grains were located over vesicles below the brush border of cells of theproximal convoluted tubules.
4 context sentences(design, population, supporting numbers)
Summary · context
These grains were still present after simultaneous injection of excess unlabeled hormone and most likely represented binding to sites involved with ingestion and degradation of hormone from the urinary filtrate.
Summary · context
An in vivo binding assay using radioautography was employed to visualize calcitonin receptors in rat tissues.
Summary · context
At 2 min after intravenous injection of biologically active 125I-salmon calcitonin, free hormone was separated from bound hormone by intracardiac perfusion with lactated Ringer's followed by fixation with 2.5% glutaraldehyde.
Summary · context
These were compared to tissues removed from animals that received identical amounts of labeled hormone with a large excess of unlabeled calcitonin.
In this study, Calcitonin-salmon was a lab tool or a carrier, part of a mix, or the subject of a lab method. This result is not evidence about taking Calcitonin-salmon.
Report a problemFND-0739113701
02AnimalNot a test of Calcitonin-salmon aloneAnimal study (in vivo)Activity measurement
In rats, excess unlabeled calcitonin significantly reduced distal tubule basal labeling, indicating saturable specific binding of labeled salmon calcitonin.
In plain wordsIn rats, excess unlabeled calcitonin reduced labeled salmon calcitonin binding at distal kidney tubule surfaces.
Peptide
Calcitonin-salmon
Outcome
Not applicableSpecific distal renal tubule binding
Study
ratn: not reported in source
Rat tissues after in-vivo calcitonin injection
Comparator
Excess unlabeled calcitonin co-injection
Outcome group
Receptor activity and mechanism · Kidney and urinary
Source sentences
Summary
In contrast, grains localized to the basal surfaces of distal convoluted tubule cells were significantly reduced in number in control animals and represented sites of saturable, specific hormone binding.
3 context sentences(design, population, supporting numbers)
Summary · context
An in vivo binding assay using radioautography was employed to visualize calcitonin receptors in rat tissues.
Summary · context
At 2 min after intravenous injection of biologically active 125I-salmon calcitonin, free hormone was separated from bound hormone by intracardiac perfusion with lactated Ringer's followed by fixation with 2.5% glutaraldehyde.
Summary · context
These were compared to tissues removed from animals that received identical amounts of labeled hormone with a large excess of unlabeled calcitonin.
In this study, Calcitonin-salmon was a lab tool or a carrier, part of a mix, or the subject of a lab method. This result is not evidence about taking Calcitonin-salmon.
Report a problemFND-0739113702
03AnimalNot a test of Calcitonin-salmon aloneAnimal study (in vivo)Activity measurement
In rats, specific salmon calcitonin binding sites in bone occurred only at osteoclast peripheries.
In plain wordsIn rats, specific salmon calcitonin binding in bone occurred only at osteoclast edges.
Peptide
Calcitonin-salmon
Outcome
Not applicableOsteoclast-specific binding
Study
ratn: not reported in source
Rat tissues after in-vivo calcitonin injection
Outcome group
Receptor activity and mechanism · Musculoskeletal
Source sentences
Summary
In bone, specific binding sites were found only at the periphery of osteoclasts.
3 context sentences(design, population, supporting numbers)
Summary · context
An in vivo binding assay using radioautography was employed to visualize calcitonin receptors in rat tissues.
Summary · context
At 2 min after intravenous injection of biologically active 125I-salmon calcitonin, free hormone was separated from bound hormone by intracardiac perfusion with lactated Ringer's followed by fixation with 2.5% glutaraldehyde.
Summary · context
These were compared to tissues removed from animals that received identical amounts of labeled hormone with a large excess of unlabeled calcitonin.
In this study, Calcitonin-salmon was a lab tool or a carrier, part of a mix, or the subject of a lab method. This result is not evidence about taking Calcitonin-salmon.
Report a problemFND-0739113703
04AnimalNot a test of Calcitonin-salmon aloneAnimal study (in vivo)Activity measurement
In rats, salmon calcitonin-labeled osteoclasts occurred at resorption sites in tibia, humerus, and alveolar bone.
In plain wordsIn rats, salmon calcitonin-labeled cells occurred at bone resorption sites in tibia, humerus, and alveolar bone.
Peptide
Calcitonin-salmon
Outcome
Not applicableSkeletal localization at resorption sites
Study
ratn: not reported in source
Rat tissues after in-vivo calcitonin injection
Outcome group
Receptor activity and mechanism · Musculoskeletal
Source sentences
Summary
These labeled cells were located at resorption sites examined in tibia, humerus, and alveolar bone.
4 context sentences(design, population, supporting numbers)
Summary · context
In bone, specific binding sites were found only at the periphery of osteoclasts.
Summary · context
An in vivo binding assay using radioautography was employed to visualize calcitonin receptors in rat tissues.
Summary · context
At 2 min after intravenous injection of biologically active 125I-salmon calcitonin, free hormone was separated from bound hormone by intracardiac perfusion with lactated Ringer's followed by fixation with 2.5% glutaraldehyde.
Summary · context
These were compared to tissues removed from animals that received identical amounts of labeled hormone with a large excess of unlabeled calcitonin.
In this study, Calcitonin-salmon was a lab tool or a carrier, part of a mix, or the subject of a lab method. This result is not evidence about taking Calcitonin-salmon.
Report a problemFND-0739113704

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Version history

PepBank keeps every version of the data on this study. Status changes from Retraction Watch, Europe PMC and Crossref are listed with their source.

Version 2Current
28 Oct 2026, 02:03 UTC
Study results updated
Version 1
27 Oct 2026, 16:35 UTC
First added · source update from Europe PMC

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