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Study · Other design · 1980

3 results

Production of biologically active N alpha-desacetylthymosin alpha 1 in Escherichia coli through expression of a chemically synthesized gene.

Wetzel R, Heyneker HL, Goeddel DV, Jhurani P, Shapiro J, Crea R, Low TL, McClure JE, Thurman GB, Goldstein AL

Biochemistry1 Dec 1980Cited by 43 (Europe PMC)

PMID7008828 (opens in a new tab)DOI10.1021/bi00567a023 (opens in a new tab)PepBankPUB-07008828
Publisher pagePubMedAsk about this study
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Summary

The text of this summary is not shown here, because the article license does not allow reuse. Read it at the source (opens in a new tab)

Text rights: Publisher copyright. Results still quote short attributed sentences.

Results from this study3

1 result from studies that gave the peptide. No result from this study comes from people.

  1. 01LabOther designActivity measurement

    In guinea pig MIF, TdT and radioimmunoassay tests, N alpha-desacetylthymosin alpha 1 made by DNA cloning had biological activity equivalent to the native hormone.

    In plain wordsIn lab tests, the bacterially made desacetyl thymosin alpha‑1 was as biologically active as the native hormone.

    Peptide
    Thymosin alpha-1
    Outcome
    No significant changeBiological activity in MIF, TdT and radioimmunoassay versus native hormone
    Study

    n: not reported in source

    E. coli recombinant product
    Comparator
    Native thymosin alpha 1
    Outcome group
    Immune response · Immune and inflammation
    Studied dose
    Dose not stated

    Source sentences

    Summary
    Results of a guinea pig migration inhibition factor (MIF) assay, a terminal deoxyribonucleotidyl transferase (TdT) assay, and radioimmunoassay indicate that the N alpha-desacetylthymosin alpha 1 produced by deoxyribonucleic acid (DNA) cloning techniques has biological activity equivalent to that of the native hormone.

Studies that did not test the peptide alone2

In this study, Thymosin alpha-1 was a lab tool or a carrier, part of a mix, or the subject of a lab method. These results are not evidence about taking Thymosin alpha-1.

  1. 02LabNot a test of Thymosin alpha-1 aloneIn vitro (cell-free)Other

    Cyanogen bromide cleavage of the E. coli fusion protein gave a polypeptide mixture in which thymosin alpha‑1 activity was detected by radioimmunoassay.

    In plain wordsIn lab tests, thymosin alpha‑1 activity was detected by radioimmunoassay in the cleaved bacterial fusion protein.

    Peptide
    Thymosin alpha-1
    Outcome
    Not applicableDetection of thymosin alpha 1 activity after cleavage of the fusion protein
    Study

    n: not reported in source

    E. coli recombinant product
    Outcome group
    Peptide structure and chemistry · Pharmacology

    Source sentences

    Summary
    Cyanogen bromide cleavage of this protein gave a mixture of polypeptides, among which thymosin alpha 1 activity was detected by radioimmunoassay (RIA).

    In this study, Thymosin alpha-1 was a lab tool or a carrier, part of a mix, or the subject of a lab method. This result is not evidence about taking Thymosin alpha-1.

  2. 03LabNot a test of Thymosin alpha-1 aloneIn vitro (cell-free)Other

    The E. coli product matched native thymosin alpha‑1 in amino acid sequence but lacked the N-terminal acetyl group.

    In plain wordsIn lab tests, the bacterial product had the same sequence as native thymosin alpha‑1 but no N-terminal acetyl group.

    Peptide
    Thymosin alpha-1
    Outcome
    No significant changeAmino acid sequence of the E. coli product versus native thymosin alpha 1
    Study

    n: not reported in source

    E. coli recombinant product
    Comparator
    Native thymosin alpha 1 from calf thymus
    Outcome group
    Peptide structure and chemistry · Pharmacology

    Source sentences

    Summary
    The E. coli product is identical with native thymosin alpha 1 isolated from calf thymus in the amino acid sequence but lacks the N-terminal acetyl group.

    In this study, Thymosin alpha-1 was a lab tool or a carrier, part of a mix, or the subject of a lab method. This result is not evidence about taking Thymosin alpha-1.

AI wrote and checked these results. AI can make mistakes, so check important results against the source.

Version history

PepBank keeps every version of the data on this study. Status changes from Retraction Watch, Europe PMC and Crossref are listed with their source.

  1. Version 3Current

    10 Oct 2026, 08:55 UTC

    Study results updated

  2. Version 2

    9 Oct 2026, 03:03 UTC

    Study data updated · source update from Europe PMC

  3. Version 1

    9 Oct 2026, 03:02 UTC

    First added · source update from Europe PMC

Subjects

Terms as the source lists them.

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