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Study · In vitro (cell-free) · 1982

7 results

High affinity binding sites on plasma membrane obtained from the lymphoblastoid cultured 1301 cell line for highly radioactive serum thymic factor.

Gastinel LN, Pleau JM, Dardenne M, Sasaki A, Bricas E, Morgat JL, Bach JF

Biochimica et biophysica acta1 Jan 1982Cited by 10 (Europe PMC)

PMID6976796 (opens in a new tab)DOI10.1016/0005-2736(82)90055-4 (opens in a new tab)PepBankPUB-06976796
Publisher pagePubMedAsk about this study
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Summary

The text of this summary is not shown here, because the article license does not allow reuse. Read it at the source (opens in a new tab)

Text rights: Publisher copyright. Results still quote short attributed sentences.

Results from this study7

7 results from studies that gave the peptide. No result from this study comes from people.

  1. 01LabIn vitro (cell-free)Mechanism

    In human T-cell membrane preparations, FTS binding depended on temperature and concentrations of labelled FTS and membrane proteins.

    In plain wordsIn lab tests, FTS binding depended on temperature and the amounts of FTS and membrane proteins.

    Peptide
    Thymulin (serum thymic factor)
    Outcome
    Not applicableTemperature and concentration dependence of binding
    Study

    humann: not reported in source

    Plasma membranes from lymphoblastoid T cell line 1301
    Outcome group
    Receptor activity and mechanism · Pharmacology
    Studied dose
    Dose not stated

    Source sentences

    Summary
    The binding is temperature dependent and function of the concentration of both 3H-labelled FTS and membrane proteins.
    1 context sentence
    Summary · context
    The interaction of the synthetic serum thymic factor (FTS, facteur thymique sérique) with a plasma membrane preparation of human T lymphocytes from the lymphoblastoid T cell line 1301 was studied using 3H-labelled FTS (specific activity 120 Ci/mmol).
  2. 02LabIn vitro (cell-free)Mechanism

    In human T-cell membrane preparations at 37 degrees C, labelled FTS binding reached steady state within 80 min.

    In plain wordsIn lab tests at 37 degrees C, FTS binding reached a steady state within 80 min.

    Peptide
    Thymulin (serum thymic factor)
    Outcome
    Not applicableTime to binding steady state
    Study

    humann: not reported in source

    Plasma membranes from lymphoblastoid T cell line 1301
    Outcome group
    Receptor activity and mechanism · Pharmacology
    Studied dose
    1 nMAs given in this study (human).

    Measurements

    Steady-state time
    80 min
    37 degrees C; 1 nM labelled FTS

    Source sentences

    Summary
    At 37 degrees C, using 1 nM of 3H-labelled FTS as steady state is observed within 80 min.
    1 context sentence
    Summary · context
    The interaction of the synthetic serum thymic factor (FTS, facteur thymique sérique) with a plasma membrane preparation of human T lymphocytes from the lymphoblastoid T cell line 1301 was studied using 3H-labelled FTS (specific activity 120 Ci/mmol).
  3. 03LabIn vitro (cell-free)Mechanism

    In human T-cell membrane preparations, FTS binding was reversible, specific and saturable.

    In plain wordsIn lab tests, FTS binding was reversible, specific and saturable.

    Peptide
    Thymulin (serum thymic factor)
    Outcome
    Not applicableBinding reversibility, specificity and saturation
    Study

    humann: not reported in source

    Plasma membranes from lymphoblastoid T cell line 1301
    Outcome group
    Receptor activity and mechanism · Pharmacology
    Studied dose
    Dose not stated

    Source sentences

    Summary
    The binding is reversible, specific and saturable.
    1 context sentence
    Summary · context
    The interaction of the synthetic serum thymic factor (FTS, facteur thymique sérique) with a plasma membrane preparation of human T lymphocytes from the lymphoblastoid T cell line 1301 was studied using 3H-labelled FTS (specific activity 120 Ci/mmol).
  4. 04LabIn vitro (cell-free)Mechanism

    In human T-cell membrane preparations, the higher-affinity FTS binding site had a Kd of approximately 0.516 nM.

    In plain wordsIn lab tests, one FTS binding site had a binding constant of approximately 0.516 nM.

    Peptide
    Thymulin (serum thymic factor)
    Outcome
    Not applicableHigher-affinity binding site Kd
    Study

    humann: not reported in source

    Plasma membranes from lymphoblastoid T cell line 1301
    Outcome group
    Receptor activity and mechanism · Pharmacology
    Studied dose
    Dose not stated

    Measurements

    Kd
    ≈ 0.516 nM
    higher-affinity binding site

    Source sentences

    Summary
    Scatchard analysis reveals the existence of at least two binding sites with respective Kd of the order of 0.516 +/- 0.2 nM and 110 +/- 27.8 nM with concentration of 0.186 +/- 0.045 pmol and 2.026 +/- 0.367 pmol per mg of membrane protein.
    1 context sentence
    Summary · context
    The interaction of the synthetic serum thymic factor (FTS, facteur thymique sérique) with a plasma membrane preparation of human T lymphocytes from the lymphoblastoid T cell line 1301 was studied using 3H-labelled FTS (specific activity 120 Ci/mmol).
  5. 05LabIn vitro (cell-free)Mechanism

    In human T-cell membrane preparations, the lower-affinity FTS binding site had a Kd of approximately 110 nM.

    In plain wordsIn lab tests, the other FTS binding site had a binding constant of approximately 110 nM.

    Peptide
    Thymulin (serum thymic factor)
    Outcome
    Not applicableLower-affinity binding site Kd
    Study

    humann: not reported in source

    Plasma membranes from lymphoblastoid T cell line 1301
    Outcome group
    Receptor activity and mechanism · Pharmacology
    Studied dose
    Dose not stated

    Measurements

    Kd
    ≈ 110 nM
    lower-affinity binding site

    Source sentences

    Summary
    Scatchard analysis reveals the existence of at least two binding sites with respective Kd of the order of 0.516 +/- 0.2 nM and 110 +/- 27.8 nM with concentration of 0.186 +/- 0.045 pmol and 2.026 +/- 0.367 pmol per mg of membrane protein.
    1 context sentence
    Summary · context
    The interaction of the synthetic serum thymic factor (FTS, facteur thymique sérique) with a plasma membrane preparation of human T lymphocytes from the lymphoblastoid T cell line 1301 was studied using 3H-labelled FTS (specific activity 120 Ci/mmol).
  6. 06LabIn vitro (cell-free)Mechanism

    In human T-cell membrane preparations, the higher-affinity FTS binding site concentration was 0.186 pmol per mg membrane protein.

    In plain wordsIn lab tests, the higher-affinity FTS binding site concentration was 0.186 pmol per mg membrane protein.

    Peptide
    Thymulin (serum thymic factor)
    Outcome
    Not applicableHigher-affinity binding site concentration
    Study

    humann: not reported in source

    Plasma membranes from lymphoblastoid T cell line 1301
    Outcome group
    Receptor activity and mechanism · Pharmacology
    Studied dose
    Dose not stated

    Measurements

    Binding site concentration
    0.186 pmol per mg
    higher-affinity binding site

    Source sentences

    Summary
    Scatchard analysis reveals the existence of at least two binding sites with respective Kd of the order of 0.516 +/- 0.2 nM and 110 +/- 27.8 nM with concentration of 0.186 +/- 0.045 pmol and 2.026 +/- 0.367 pmol per mg of membrane protein.
    1 context sentence
    Summary · context
    The interaction of the synthetic serum thymic factor (FTS, facteur thymique sérique) with a plasma membrane preparation of human T lymphocytes from the lymphoblastoid T cell line 1301 was studied using 3H-labelled FTS (specific activity 120 Ci/mmol).
  7. 07LabIn vitro (cell-free)Mechanism

    In human T-cell membrane preparations, the lower-affinity FTS binding site concentration was 2.026 pmol per mg membrane protein.

    In plain wordsIn lab tests, the lower-affinity FTS binding site concentration was 2.026 pmol per mg membrane protein.

    Peptide
    Thymulin (serum thymic factor)
    Outcome
    Not applicableLower-affinity binding site concentration
    Study

    humann: not reported in source

    Plasma membranes from lymphoblastoid T cell line 1301
    Outcome group
    Receptor activity and mechanism · Pharmacology
    Studied dose
    Dose not stated

    Measurements

    Binding site concentration
    2.026 pmol per mg
    lower-affinity binding site

    Source sentences

    Summary
    Scatchard analysis reveals the existence of at least two binding sites with respective Kd of the order of 0.516 +/- 0.2 nM and 110 +/- 27.8 nM with concentration of 0.186 +/- 0.045 pmol and 2.026 +/- 0.367 pmol per mg of membrane protein.
    1 context sentence
    Summary · context
    The interaction of the synthetic serum thymic factor (FTS, facteur thymique sérique) with a plasma membrane preparation of human T lymphocytes from the lymphoblastoid T cell line 1301 was studied using 3H-labelled FTS (specific activity 120 Ci/mmol).

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Version history

PepBank keeps every version of the data on this study. Status changes from Retraction Watch, Europe PMC and Crossref are listed with their source.

  1. Version 2Current

    21 Oct 2026, 15:58 UTC

    Study results updated

  2. Version 1

    21 Oct 2026, 12:50 UTC

    First added · source update from Europe PMC

Subjects

Terms as the source lists them.

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