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Study · Not a test of the peptide alone · 1984

2 results

HPLC shadowing: artifacts in peptide characterization monitored by RIA.

Fischman AJ, Kastin AJ, Graf MV

Peptides1 Sept 1984Cited by 18 (Europe PMC)

PMID6548808 (opens in a new tab)DOI10.1016/0196-9781(84)90128-1 (opens in a new tab)PepBankPUB-06548808
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Summary

The text of this summary is not shown here, because the article license does not allow reuse. Read it at the source (opens in a new tab)

Text rights: Publisher copyright. Results still quote short attributed sentences.

Results from this study2

No result from this study comes from a test of the peptide.

Studies that did not test the peptide alone2

In this study, Delta sleep-inducing peptide (DSIP) was a lab tool or a carrier, part of a mix, or the subject of a lab method. These results are not evidence about taking Delta sleep-inducing peptide (DSIP).

  1. 01LabNot a test of Delta sleep-inducing peptide (DSIP) aloneIn vitro (cell-free)Other

    In HPLC-RIA of DSIP, carry-over (shadowing) from the previous standard was as high as 10%, which can cause false positive results.

    In plain wordsIn lab tests, up to 10% of DSIP carried over between HPLC runs, which can give false positive results.

    Peptide
    Delta sleep-inducing peptide (DSIP)
    Outcome
    Not applicableHPLC shadowing of DSIP
    Study

    n: not reported in source

    HPLC with radioimmunoassay
    Outcome group
    Analytical methods and detection · Pharmacology

    Measurements

    HPLC shadowing
    ≤ 10%
    DSIP

    Source sentences

    Summary
    With delta sleep-inducing peptide (DSIP), we found the shadowing to be as high as 10%, although it was only 1% with 125I-Tyr-DSIP.
    1 context sentence
    Summary · context
    The shadow phenomenon, representing carry-over of the peptide from previous application of the standard, can appear to result in the presence of endogenous peptide in the test sample when none is actually there.

    In this study, Delta sleep-inducing peptide (DSIP) was a lab tool or a carrier, part of a mix, or the subject of a lab method. This result is not evidence about taking Delta sleep-inducing peptide (DSIP).

  2. 02LabNot a test of Delta sleep-inducing peptide (DSIP) aloneIn vitro (cell-free)Other

    In HPLC-RIA, shadowing of 125I-Tyr-DSIP was only 1%.

    In plain wordsIn lab tests, only 1% of labeled DSIP carried over between HPLC runs.

    Peptide
    Delta sleep-inducing peptide (DSIP)
    Outcome
    Not applicableHPLC shadowing of 125I-Tyr-DSIP
    Study

    n: not reported in source

    HPLC with radioimmunoassay
    Outcome group
    Analytical methods and detection · Pharmacology

    Measurements

    HPLC shadowing
    1%
    125I-Tyr-DSIP

    Source sentences

    Summary
    With delta sleep-inducing peptide (DSIP), we found the shadowing to be as high as 10%, although it was only 1% with 125I-Tyr-DSIP.

    In this study, Delta sleep-inducing peptide (DSIP) was a lab tool or a carrier, part of a mix, or the subject of a lab method. This result is not evidence about taking Delta sleep-inducing peptide (DSIP).

AI wrote and checked these results. AI can make mistakes, so check important results against the source.

Version history

PepBank keeps every version of the data on this study. Status changes from Retraction Watch, Europe PMC and Crossref are listed with their source.

  1. Version 2Current

    10 Oct 2026, 09:43 UTC

    Study results updated

  2. Version 1

    9 Oct 2026, 03:04 UTC

    First added · source update from Europe PMC

Subjects

Terms as the source lists them.

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