Oxytocin resistance in Brattleboro rat adipocytes and comparative studies on insulin or oxytocin responsiveness in normal rat adipocytes.

Hanif K, Goren HJ, Geonzon RM, Lederis K, Hollenberg MD

Canadian journal of physiology and pharmacology1 Nov 1983Cited by 2 (Europe PMC)

PMID6362806DOI10.1139/y83-202PepBankPUB-06362806

Publisher page PubMedAsk about this study

Summary

The text of this summary is not shown here, because the article license does not allow reuse. Read it at the source (opens in a new tab)

Text rights: Publisher copyright. Results still quote short attributed sentences.

Results from this study18

2 results from studies that gave the peptide. No result from this study comes from people.

01LabEx vivo tissueEfficacy outcome
In estrogenized Brattleboro rat uteri, oxytocin contractile responses were indistinguishable from those in estrogenized normal rats.
In plain wordsIn lab tests of estrogenized Brattleboro rat uterine tissue, oxytocin responses were similar to those in estrogenized normal rat tissue.
Peptide
Oxytocin
Outcome
No significant changeOxytocin-induced uterine contractile response
Study
ratn: not reported in source
estrogenized Brattleboro rat uterus
Comparator
Estrogenized normal rat uterus
Outcome group
Sex hormones and fertility · Reproductive and sexual health
Studied dose
Dose not stated
Source sentences
Summary
In contrast, the contractile responses of estrogenized HoDI rat uteri were indistinguishable from those of estrogenized normal rats.
1 context sentence(design, population, supporting numbers)
Summary · context
lack of an oxytocin-mediated insulinlike response (glucose oxidation; lipogenesis) in adipocytes from Brattleboro rats, homozygous for the diabetes insipidus trait (HoDI rats).
Report a problemFND-0636280602
02LabIn vitro (cells)Efficacy outcome
In Long Evans or Sprague-Dawley rat adipocytes, maximum oxytocin responsiveness was less than 20% of the maximal insulin response.
In plain wordsIn lab tests of rat fat cells, the largest oxytocin response was less than 20% of the largest insulin response.
Peptide
Oxytocin
Outcome
DecreaseMaximum oxytocin responsiveness compared with insulin
Study
ratn: not reported in source
Long Evans or Sprague-Dawley rat adipocytes
Comparator
Maximum insulin response
Outcome group
Glycemic control · Metabolic and weight
Studied dose
Dose not stated
Measurements
Maximum oxytocin response
< 20%
of maximal insulin response
Source sentences
Summary
Nonetheless, the maximum oxytocin responsiveness of adipocytes from Long Evans or Sprague-Dawley rats, under all conditions of assay, was still only a fraction (less than 20%) of the maximal response to insulin.
Report a problemFND-0636280604

Studies that did not test the peptide alone16

In this study, Oxytocin was a lab tool or a carrier, part of a mix, or the subject of a lab method. These results are not evidence about taking Oxytocin.

03LabNot a test of Oxytocin aloneIn vitro (cells)Mechanism
In HoDI Brattleboro rat adipocytes, increased glucose in the fat-pad digestion medium failed to restore oxytocin activation of glucose oxidation.
In plain wordsIn lab tests of HoDI rat fat cells, increased glucose did not restore oxytocin-related glucose oxidation.
Peptide
Oxytocin
Outcome
No significant changeOxytocin-mediated glucose oxidation after increased glucose in the fat-pad digestion medium
Study
ratn: not reported in source
HoDI Brattleboro rat adipocytes
Outcome group
Glycemic control · Metabolic and weight
Source sentences
Summary
increased glucose in the fat pad digestion medium; increased calcium concentration in the oxidation assay; estrogen treatment; use of [1-14C]glucose as substrate; inclusion of adenosine in the assay medium; vasopressin replacement therapy) uniformly failed to result in oxytocin activation of HoDI adipocytes.
1 context sentence(design, population, supporting numbers)
Summary · context
We have evaluated factors, other than genetic, which might be related to the lack of an oxytocin-mediated insulinlike response (glucose oxidation; lipogenesis) in adipocytes from Brattleboro rats, homozygous for the diabetes insipidus trait (HoDI rats).
In this study, Oxytocin was a lab tool or a carrier, part of a mix, or the subject of a lab method. This result is not evidence about taking Oxytocin.
Report a problemFND-0636280601
04LabNot a test of Oxytocin aloneIn vitro (cells)Mechanism
In normal Sprague-Dawley or Long Evans rat adipocytes, calcium (10 mM) in the assay medium augmented oxytocin responsiveness.
In plain wordsIn lab tests of normal rat fat cells, calcium in the test medium increased responsiveness to oxytocin.
Peptide
Oxytocin
Outcome
IncreaseOxytocin responsiveness after calcium in assay medium
Study
ratn: not reported in source
normal rat adipocytes
Outcome group
Glycemic control · Metabolic and weight
Source sentences
Summary
On the other hand, in normal fat cells (from Sprague-Dawley and Long Evans rats), oxytocin responsiveness was augmented by a number of the manoeuvres mentioned above, most notably by the inclusion of either calcium (10 mM) or adenosine (10 microM) in the assay medium.
In this study, Oxytocin was a lab tool or a carrier, part of a mix, or the subject of a lab method. This result is not evidence about taking Oxytocin.
Report a problemFND-0636280603
05LabNot a test of Oxytocin aloneIn vitro (cells)Mechanism
In normal rat adipocytes, adenosine increased sensitivity to oxytocin.
In plain wordsIn lab tests of normal rat fat cells, adenosine increased sensitivity to oxytocin.
Peptide
Oxytocin
Outcome
IncreaseOxytocin sensitivity after adenosine
Study
ratn: not reported in source
normal rat adipocytes
Comparator
Without adenosine
Outcome group
Glycemic control · Metabolic and weight
Source sentences
Summary
The effect of adenosine on oxytocin action (increased sensitivity, without an effect on the maximum response) is in keeping with the previously observed effects of this nucleoside on the action of insulin; our results thus pointed to a new parallel in the action of insulin and oxytocin.
1 context sentence(design, population, supporting numbers)
Summary · context
On the other hand, in normal fat cells (from Sprague-Dawley and Long Evans rats), oxytocin responsiveness was augmented by a number of the manoeuvres mentioned above, most notably by the inclusion of either calcium (10 mM) or adenosine (10 microM) in the assay medium.
In this study, Oxytocin was a lab tool or a carrier, part of a mix, or the subject of a lab method. This result is not evidence about taking Oxytocin.
Report a problemFND-0636280605
06LabNot a test of Oxytocin aloneIn vitro (cells)Mechanism
In normal rat adipocytes, adenosine had no effect on the maximum oxytocin response.
In plain wordsIn lab tests of normal rat fat cells, adenosine did not change the largest response to oxytocin.
Peptide
Oxytocin
Outcome
No significant changeMaximum oxytocin response after adenosine
Study
ratn: not reported in source
normal rat adipocytes
Comparator
Without adenosine
Outcome group
Glycemic control · Metabolic and weight
Source sentences
Summary
The effect of adenosine on oxytocin action (increased sensitivity, without an effect on the maximum response) is in keeping with the previously observed effects of this nucleoside on the action of insulin; our results thus pointed to a new parallel in the action of insulin and oxytocin.
1 context sentence(design, population, supporting numbers)
Summary · context
On the other hand, in normal fat cells (from Sprague-Dawley and Long Evans rats), oxytocin responsiveness was augmented by a number of the manoeuvres mentioned above, most notably by the inclusion of either calcium (10 mM) or adenosine (10 microM) in the assay medium.
In this study, Oxytocin was a lab tool or a carrier, part of a mix, or the subject of a lab method. This result is not evidence about taking Oxytocin.
Report a problemFND-0636280606
07LabNot a test of Oxytocin aloneIn vitro (cells)Mechanism
In normal Sprague-Dawley or Long Evans rat adipocytes, adenosine (10 microM) in the assay medium augmented oxytocin responsiveness.
In plain wordsIn lab tests of normal rat fat cells, adenosine in the test medium increased responsiveness to oxytocin.
Peptide
Oxytocin
Outcome
IncreaseOxytocin responsiveness after adenosine in assay medium
Study
ratn: not reported in source
normal rat adipocytes
Outcome group
Glycemic control · Metabolic and weight
Source sentences
Summary
On the other hand, in normal fat cells (from Sprague-Dawley and Long Evans rats), oxytocin responsiveness was augmented by a number of the manoeuvres mentioned above, most notably by the inclusion of either calcium (10 mM) or adenosine (10 microM) in the assay medium.
In this study, Oxytocin was a lab tool or a carrier, part of a mix, or the subject of a lab method. This result is not evidence about taking Oxytocin.
Report a problemFND-0636280607
08LabNot a test of Oxytocin aloneIn vitro (cells)Mechanism
In HoDI Brattleboro rat adipocytes, increased glucose in the fat-pad digestion medium failed to restore oxytocin activation of lipogenesis.
In plain wordsIn lab tests of HoDI rat fat cells, increased glucose did not restore oxytocin-related lipogenesis.
Peptide
Oxytocin
Outcome
No significant changeOxytocin-mediated lipogenesis after increased glucose in the fat-pad digestion medium
Study
ratn: not reported in source
HoDI Brattleboro rat adipocytes
Outcome group
Glycemic control · Metabolic and weight
Source sentences
Summary
increased glucose in the fat pad digestion medium; increased calcium concentration in the oxidation assay; estrogen treatment; use of [1-14C]glucose as substrate; inclusion of adenosine in the assay medium; vasopressin replacement therapy) uniformly failed to result in oxytocin activation of HoDI adipocytes.
1 context sentence(design, population, supporting numbers)
Summary · context
We have evaluated factors, other than genetic, which might be related to the lack of an oxytocin-mediated insulinlike response (glucose oxidation; lipogenesis) in adipocytes from Brattleboro rats, homozygous for the diabetes insipidus trait (HoDI rats).
In this study, Oxytocin was a lab tool or a carrier, part of a mix, or the subject of a lab method. This result is not evidence about taking Oxytocin.
Report a problemFND-0636280608
09LabNot a test of Oxytocin aloneIn vitro (cells)Mechanism
In HoDI Brattleboro rat adipocytes, increased calcium concentration in the oxidation assay failed to restore oxytocin activation of glucose oxidation.
In plain wordsIn lab tests of HoDI rat fat cells, increased calcium did not restore oxytocin-related glucose oxidation.
Peptide
Oxytocin
Outcome
No significant changeOxytocin-mediated glucose oxidation after increased calcium in the oxidation assay
Study
ratn: not reported in source
HoDI Brattleboro rat adipocytes
Outcome group
Glycemic control · Metabolic and weight
Source sentences
Summary
increased calcium concentration in the oxidation assay; estrogen treatment; use of [1-14C]glucose as substrate; inclusion of adenosine in the assay medium; vasopressin replacement therapy) uniformly failed to result in oxytocin activation of HoDI adipocytes.
1 context sentence(design, population, supporting numbers)
Summary · context
We have evaluated factors, other than genetic, which might be related to the lack of an oxytocin-mediated insulinlike response (glucose oxidation; lipogenesis) in adipocytes from Brattleboro rats, homozygous for the diabetes insipidus trait (HoDI rats).
In this study, Oxytocin was a lab tool or a carrier, part of a mix, or the subject of a lab method. This result is not evidence about taking Oxytocin.
Report a problemFND-0636280609
10LabNot a test of Oxytocin aloneIn vitro (cells)Mechanism
In HoDI Brattleboro rat adipocytes, estrogen treatment failed to restore oxytocin activation of glucose oxidation.
In plain wordsIn lab tests of HoDI rat fat cells, estrogen treatment did not restore oxytocin-related glucose oxidation.
Peptide
Oxytocin
Outcome
No significant changeOxytocin-mediated glucose oxidation after estrogen treatment
Study
ratn: not reported in source
HoDI Brattleboro rat adipocytes
Outcome group
Glycemic control · Metabolic and weight
Source sentences
Summary
estrogen treatment; use of [1-14C]glucose as substrate; inclusion of adenosine in the assay medium; vasopressin replacement therapy) uniformly failed to result in oxytocin activation of HoDI adipocytes.
1 context sentence(design, population, supporting numbers)
Summary · context
We have evaluated factors, other than genetic, which might be related to the lack of an oxytocin-mediated insulinlike response (glucose oxidation; lipogenesis) in adipocytes from Brattleboro rats, homozygous for the diabetes insipidus trait (HoDI rats).
In this study, Oxytocin was a lab tool or a carrier, part of a mix, or the subject of a lab method. This result is not evidence about taking Oxytocin.
Report a problemFND-0636280610
11LabNot a test of Oxytocin aloneIn vitro (cells)Mechanism
In HoDI Brattleboro rat adipocytes, use of [1-14C]glucose as substrate failed to restore oxytocin activation of glucose oxidation.
In plain wordsIn lab tests of HoDI rat fat cells, this glucose substrate did not restore oxytocin-related glucose oxidation.
Peptide
Oxytocin
Outcome
No significant changeOxytocin-mediated glucose oxidation with [1-14C]glucose substrate
Study
ratn: not reported in source
HoDI Brattleboro rat adipocytes
Outcome group
Glycemic control · Metabolic and weight
Source sentences
Summary
use of [1-14C]glucose as substrate; inclusion of adenosine in the assay medium; vasopressin replacement therapy) uniformly failed to result in oxytocin activation of HoDI adipocytes.
1 context sentence(design, population, supporting numbers)
Summary · context
We have evaluated factors, other than genetic, which might be related to the lack of an oxytocin-mediated insulinlike response (glucose oxidation; lipogenesis) in adipocytes from Brattleboro rats, homozygous for the diabetes insipidus trait (HoDI rats).
In this study, Oxytocin was a lab tool or a carrier, part of a mix, or the subject of a lab method. This result is not evidence about taking Oxytocin.
Report a problemFND-0636280611
12LabNot a test of Oxytocin aloneIn vitro (cells)Mechanism
In HoDI Brattleboro rat adipocytes, adenosine in the assay medium failed to restore oxytocin activation of glucose oxidation.
In plain wordsIn lab tests of HoDI rat fat cells, adenosine did not restore oxytocin-related glucose oxidation.
Peptide
Oxytocin
Outcome
No significant changeOxytocin-mediated glucose oxidation after adenosine in the assay medium
Study
ratn: not reported in source
HoDI Brattleboro rat adipocytes
Outcome group
Glycemic control · Metabolic and weight
Source sentences
Summary
inclusion of adenosine in the assay medium; vasopressin replacement therapy) uniformly failed to result in oxytocin activation of HoDI adipocytes.
1 context sentence(design, population, supporting numbers)
Summary · context
We have evaluated factors, other than genetic, which might be related to the lack of an oxytocin-mediated insulinlike response (glucose oxidation; lipogenesis) in adipocytes from Brattleboro rats, homozygous for the diabetes insipidus trait (HoDI rats).
In this study, Oxytocin was a lab tool or a carrier, part of a mix, or the subject of a lab method. This result is not evidence about taking Oxytocin.
Report a problemFND-0636280612
13LabNot a test of Oxytocin aloneIn vitro (cells)Mechanism
In HoDI Brattleboro rat adipocytes, vasopressin replacement therapy failed to restore oxytocin activation of glucose oxidation.
In plain wordsIn lab tests of HoDI rat fat cells, vasopressin replacement did not restore oxytocin-related glucose oxidation.
Peptide
Oxytocin
Outcome
No significant changeOxytocin-mediated glucose oxidation after vasopressin replacement therapy
Study
ratn: not reported in source
HoDI Brattleboro rat adipocytes
Outcome group
Glycemic control · Metabolic and weight
Source sentences
Summary
vasopressin replacement therapy) uniformly failed to result in oxytocin activation of HoDI adipocytes.
1 context sentence(design, population, supporting numbers)
Summary · context
We have evaluated factors, other than genetic, which might be related to the lack of an oxytocin-mediated insulinlike response (glucose oxidation; lipogenesis) in adipocytes from Brattleboro rats, homozygous for the diabetes insipidus trait (HoDI rats).
In this study, Oxytocin was a lab tool or a carrier, part of a mix, or the subject of a lab method. This result is not evidence about taking Oxytocin.
Report a problemFND-0636280613
14LabNot a test of Oxytocin aloneIn vitro (cells)Mechanism
In HoDI Brattleboro rat adipocytes, increased calcium concentration in the oxidation assay failed to restore oxytocin activation of lipogenesis.
In plain wordsIn lab tests of HoDI rat fat cells, increased calcium did not restore oxytocin-related lipogenesis.
Peptide
Oxytocin
Outcome
No significant changeOxytocin-mediated lipogenesis after increased calcium in the oxidation assay
Study
ratn: not reported in source
HoDI Brattleboro rat adipocytes
Outcome group
Glycemic control · Metabolic and weight
Source sentences
Summary
increased calcium concentration in the oxidation assay; estrogen treatment; use of [1-14C]glucose as substrate; inclusion of adenosine in the assay medium; vasopressin replacement therapy) uniformly failed to result in oxytocin activation of HoDI adipocytes.
1 context sentence(design, population, supporting numbers)
Summary · context
We have evaluated factors, other than genetic, which might be related to the lack of an oxytocin-mediated insulinlike response (glucose oxidation; lipogenesis) in adipocytes from Brattleboro rats, homozygous for the diabetes insipidus trait (HoDI rats).
In this study, Oxytocin was a lab tool or a carrier, part of a mix, or the subject of a lab method. This result is not evidence about taking Oxytocin.
Report a problemFND-0636280614
15LabNot a test of Oxytocin aloneIn vitro (cells)Mechanism
In HoDI Brattleboro rat adipocytes, estrogen treatment failed to restore oxytocin activation of lipogenesis.
In plain wordsIn lab tests of HoDI rat fat cells, estrogen treatment did not restore oxytocin-related lipogenesis.
Peptide
Oxytocin
Outcome
No significant changeOxytocin-mediated lipogenesis after estrogen treatment
Study
ratn: not reported in source
HoDI Brattleboro rat adipocytes
Outcome group
Glycemic control · Metabolic and weight
Source sentences
Summary
estrogen treatment; use of [1-14C]glucose as substrate; inclusion of adenosine in the assay medium; vasopressin replacement therapy) uniformly failed to result in oxytocin activation of HoDI adipocytes.
1 context sentence(design, population, supporting numbers)
Summary · context
We have evaluated factors, other than genetic, which might be related to the lack of an oxytocin-mediated insulinlike response (glucose oxidation; lipogenesis) in adipocytes from Brattleboro rats, homozygous for the diabetes insipidus trait (HoDI rats).
In this study, Oxytocin was a lab tool or a carrier, part of a mix, or the subject of a lab method. This result is not evidence about taking Oxytocin.
Report a problemFND-0636280615
16LabNot a test of Oxytocin aloneIn vitro (cells)Mechanism
In HoDI Brattleboro rat adipocytes, use of [1-14C]glucose as substrate failed to restore oxytocin activation of lipogenesis.
In plain wordsIn lab tests of HoDI rat fat cells, this glucose substrate did not restore oxytocin-related lipogenesis.
Peptide
Oxytocin
Outcome
No significant changeOxytocin-mediated lipogenesis with [1-14C]glucose substrate
Study
ratn: not reported in source
HoDI Brattleboro rat adipocytes
Outcome group
Glycemic control · Metabolic and weight
Source sentences
Summary
use of [1-14C]glucose as substrate; inclusion of adenosine in the assay medium; vasopressin replacement therapy) uniformly failed to result in oxytocin activation of HoDI adipocytes.
1 context sentence(design, population, supporting numbers)
Summary · context
We have evaluated factors, other than genetic, which might be related to the lack of an oxytocin-mediated insulinlike response (glucose oxidation; lipogenesis) in adipocytes from Brattleboro rats, homozygous for the diabetes insipidus trait (HoDI rats).
In this study, Oxytocin was a lab tool or a carrier, part of a mix, or the subject of a lab method. This result is not evidence about taking Oxytocin.
Report a problemFND-0636280616
17LabNot a test of Oxytocin aloneIn vitro (cells)Mechanism
In HoDI Brattleboro rat adipocytes, adenosine in the assay medium failed to restore oxytocin activation of lipogenesis.
In plain wordsIn lab tests of HoDI rat fat cells, adenosine did not restore oxytocin-related lipogenesis.
Peptide
Oxytocin
Outcome
No significant changeOxytocin-mediated lipogenesis after adenosine in the assay medium
Study
ratn: not reported in source
HoDI Brattleboro rat adipocytes
Outcome group
Glycemic control · Metabolic and weight
Source sentences
Summary
inclusion of adenosine in the assay medium; vasopressin replacement therapy) uniformly failed to result in oxytocin activation of HoDI adipocytes.
1 context sentence(design, population, supporting numbers)
Summary · context
We have evaluated factors, other than genetic, which might be related to the lack of an oxytocin-mediated insulinlike response (glucose oxidation; lipogenesis) in adipocytes from Brattleboro rats, homozygous for the diabetes insipidus trait (HoDI rats).
In this study, Oxytocin was a lab tool or a carrier, part of a mix, or the subject of a lab method. This result is not evidence about taking Oxytocin.
Report a problemFND-0636280617
18LabNot a test of Oxytocin aloneIn vitro (cells)Mechanism
In HoDI Brattleboro rat adipocytes, vasopressin replacement therapy failed to restore oxytocin activation of lipogenesis.
In plain wordsIn lab tests of HoDI rat fat cells, vasopressin replacement did not restore oxytocin-related lipogenesis.
Peptide
Oxytocin
Outcome
No significant changeOxytocin-mediated lipogenesis after vasopressin replacement therapy
Study
ratn: not reported in source
HoDI Brattleboro rat adipocytes
Outcome group
Glycemic control · Metabolic and weight
Source sentences
Summary
vasopressin replacement therapy) uniformly failed to result in oxytocin activation of HoDI adipocytes.
1 context sentence(design, population, supporting numbers)
Summary · context
We have evaluated factors, other than genetic, which might be related to the lack of an oxytocin-mediated insulinlike response (glucose oxidation; lipogenesis) in adipocytes from Brattleboro rats, homozygous for the diabetes insipidus trait (HoDI rats).
In this study, Oxytocin was a lab tool or a carrier, part of a mix, or the subject of a lab method. This result is not evidence about taking Oxytocin.
Report a problemFND-0636280618

AI wrote and checked these results. AI can make mistakes, so check important results against the source.

Version history

PepBank keeps every version of the data on this study. Status changes from Retraction Watch, Europe PMC and Crossref are listed with their source.

Version 2Current
11 Oct 2026, 20:03 UTC
Study results updated
Version 1
9 Oct 2026, 02:52 UTC
First added · source update from Europe PMC

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