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Study · In vitro (cells) · guinea pig · 1981

11 results

Importance of the vasoactive intestinal peptide receptor in the stimulation of cyclic adenosine 3',5'-monophosphate in gallbladder epithelial cells of man. Comparison with the guinea pig.

Dupont C, Broyart JP, Broer Y, Chenut B, Laburthe M, Rosselin G

The Journal of clinical investigation1 Mar 1981Cited by 58 (Europe PMC)

PMID6259209 (opens in a new tab)PMCIDPMC370625 (opens in a new tab)DOI10.1172/jci110091 (opens in a new tab)PepBankPUB-06259209
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Summary

The text of this summary is not shown here, because the article license does not allow reuse. Read it at the source (opens in a new tab)

Text rights: Publisher copyright. Results still quote short attributed sentences.

Results from this study11

11 results from studies that gave the peptide. No result from this study comes from people.

  1. 01LabIn vitro (cells)Activity measurement

    In isolated human gallbladder epithelial cells, Scatchard analysis showed two classes of VIP receptor sites: high affinity (Kd 1.3 nM) and low affinity (Kd 16.2 nM).

    In plain wordsIn cells from human gallbladder, VIP bound to two kinds of receptor, one with high and one with low affinity.

    Peptide
    Vasoactive intestinal peptide (VIP, aviptadil)
    Outcome
    Not applicableTwo classes of VIP binding sites
    Study

    humann: not reported in source

    isolated gallbladder epithelial cells
    Outcome group
    Receptor activity and mechanism · Gastrointestinal
    Studied dose
    Dose not stated

    Measurements

    Kd
    1.3 nM
    high affinity sites
    Kd
    16.2 nM
    low affinity sites

    Source sentences

    Summary
    Scatchard analysis indicated two functionally independent classes of receptor sites: 62,000 high affinity sites/cell with a dissociation constant (K(d)) of 1.3 nM, and 510,000 low affinity sites/cell with a K(d) of 16.2 nM.
  2. 02LabIn vitro (cells)Activity measurement

    In isolated human gallbladder epithelial cells, native VIP inhibited binding of labeled VIP with half-maximal inhibition at 2 nM.

    In plain wordsIn cells from human gallbladder, unlabeled VIP blocked binding of labeled VIP, with half the maximum block at 2 nM.

    Peptide
    Vasoactive intestinal peptide (VIP, aviptadil)
    Outcome
    Not applicableHalf-maximal inhibition of tracer binding by native VIP
    Study

    humann: not reported in source

    isolated gallbladder epithelial cells
    Outcome group
    Receptor activity and mechanism · Gastrointestinal
    Studied dose
    Dose not stated

    Measurements

    Half-maximal inhibition
    2 nM
    native VIP, 125I-VIP binding

    Source sentences

    Summary
    Binding of the labeled peptide was inhibited by concentrations of native VIP of 30 pM-0.1 muM. Half-maximal inhibition was observed at 2 nM.
    1 context sentence
    Summary · context
    An EDTA procedure was used to prepare isolated epithelial cells of human gallbladder devoid of endogenous vasoactive intestinal peptide (VIP) as measured by radioimmunoassay.
  3. 03LabIn vitro (cells)Activity measurement

    In isolated human gallbladder epithelial cells, secretin inhibited VIP tracer binding with 1,000 times lower potency than native VIP.

    In plain wordsIn cells from human gallbladder, secretin blocked VIP binding about 1,000 times less strongly than VIP itself.

    Peptide
    Vasoactive intestinal peptide (VIP, aviptadil)
    Outcome
    Not applicableSecretin potency for inhibiting VIP binding
    Study

    humann: not reported in source

    isolated gallbladder epithelial cells
    Comparator
    Secretin
    Outcome group
    Receptor activity and mechanism · Gastrointestinal
    Studied dose
    Dose not stated

    Source sentences

    Summary
    Secretin inhibited tracer binding but with a 1,000 times lower potency than native VIP.
    1 context sentence
    Summary · context
    An EDTA procedure was used to prepare isolated epithelial cells of human gallbladder devoid of endogenous vasoactive intestinal peptide (VIP) as measured by radioimmunoassay.
  4. 04LabIn vitro (cells)Efficacy outcome

    In isolated human gallbladder epithelial cells at 37 degrees C, 0.1 nM VIP raised cyclic AMP 44 times above the basal level.

    In plain wordsIn cells from human gallbladder, 0.1 nM VIP raised cyclic AMP 44 times above the basal level.

    Peptide
    Vasoactive intestinal peptide (VIP, aviptadil)
    Outcome
    IncreaseCyclic AMP production with 0.1 nM VIP
    Study

    humann: not reported in source

    isolated gallbladder epithelial cells
    Comparator
    Basal level
    Outcome group
    Receptor activity and mechanism · Gastrointestinal
    Studied dose
    0.1 nMAs given in this study (human).

    Measurements

    Cyclic AMP fold increase
    44 x basal
    0.1 nM VIP, 37 degrees C

    Source sentences

    Summary
    At 37 degrees C, 0.1 nM and 10 nM VIP raised cyclic AMP levels 44 and 100 times above the basal level, respectively.
    1 context sentence
    Summary · context
    An EDTA procedure was used to prepare isolated epithelial cells of human gallbladder devoid of endogenous vasoactive intestinal peptide (VIP) as measured by radioimmunoassay.
  5. 05LabIn vitro (cells)Efficacy outcome

    In isolated human gallbladder epithelial cells at 37 degrees C, 10 nM VIP raised cyclic AMP 100 times above the basal level.

    In plain wordsIn cells from human gallbladder, 10 nM VIP raised cyclic AMP 100 times above the basal level.

    Peptide
    Vasoactive intestinal peptide (VIP, aviptadil)
    Outcome
    IncreaseCyclic AMP production with 10 nM VIP
    Study

    humann: not reported in source

    isolated gallbladder epithelial cells
    Comparator
    Basal level
    Outcome group
    Receptor activity and mechanism · Gastrointestinal
    Studied dose
    10 nMAs given in this study (human).

    Measurements

    Cyclic AMP fold increase
    100 x basal
    10 nM VIP, 37 degrees C

    Source sentences

    Summary
    At 37 degrees C, 0.1 nM and 10 nM VIP raised cyclic AMP levels 44 and 100 times above the basal level, respectively.
    1 context sentence
    Summary · context
    An EDTA procedure was used to prepare isolated epithelial cells of human gallbladder devoid of endogenous vasoactive intestinal peptide (VIP) as measured by radioimmunoassay.
  6. 06LabIn vitro (cells)Efficacy outcome

    In isolated human gallbladder epithelial cells, cyclic AMP production was noted at VIP concentrations as low as 3 pM during 60-minute incubation at 15 degrees C.

    In plain wordsIn cells from human gallbladder, VIP raised cyclic AMP even at a very low concentration of 3 pM.

    Peptide
    Vasoactive intestinal peptide (VIP, aviptadil)
    Outcome
    IncreaseLowest VIP concentration stimulating cyclic AMP
    Study

    humann: not reported in source

    isolated gallbladder epithelial cells
    Outcome group
    Receptor activity and mechanism · Gastrointestinal
    Studied dose
    3 pMAs given in this study (human).

    Measurements

    Lowest effective VIP concentration
    3 pM
    15 degrees C, 60 min

    Source sentences

    Summary
    At equilibrium, during a 60-min incubation at 15 degrees C, cyclic AMP production was noted at concentrations of VIP as low as 3 pM.
    1 context sentence
    Summary · context
    An EDTA procedure was used to prepare isolated epithelial cells of human gallbladder devoid of endogenous vasoactive intestinal peptide (VIP) as measured by radioimmunoassay.
  7. 07LabIn vitro (cells)Efficacy outcome

    In isolated human gallbladder epithelial cells, VIP caused maximal cyclic AMP stimulation at 10 nM and half-maximal stimulation at 0.2 nM.

    In plain wordsIn cells from human gallbladder, VIP gave its maximum cyclic AMP rise at 10 nM and half of it at 0.2 nM.

    Peptide
    Vasoactive intestinal peptide (VIP, aviptadil)
    Outcome
    IncreaseMaximal and half-maximal cyclic AMP stimulation by VIP
    Study

    humann: not reported in source

    isolated gallbladder epithelial cells
    Outcome group
    Receptor activity and mechanism · Gastrointestinal
    Studied dose
    Dose not stated

    Measurements

    Maximal stimulation concentration
    10 nM
    VIP
    Half-maximal stimulation concentration
    0.2 nM
    VIP

    Source sentences

    Summary
    Maximal and half-maximal stimulations were observed at 10 nM and 0.2 nM VIP, respectively.
    1 context sentence
    Summary · context
    An EDTA procedure was used to prepare isolated epithelial cells of human gallbladder devoid of endogenous vasoactive intestinal peptide (VIP) as measured by radioimmunoassay.
  8. 08LabIn vitro (cells)Mechanism

    In isolated human gallbladder epithelial cells, the VIP-induced cyclic AMP rise was related, at least in part, to low phosphodiesterase activity.

    In plain wordsIn cells from human gallbladder, VIP raised cyclic AMP strongly, partly because the cells break cyclic AMP down slowly.

    Peptide
    Vasoactive intestinal peptide (VIP, aviptadil)
    Outcome
    Not applicableLow phosphodiesterase activity and VIP-induced cyclic AMP rise
    Study

    humann: not reported in source

    isolated gallbladder epithelial cells
    Outcome group
    Receptor activity and mechanism · Gastrointestinal
    Studied dose
    Dose not stated

    Source sentences

    Summary
    The importance of the VIP-induced cyclic AMP rise was related, at least in part, to a low phosphodiesterase activity in human gallbladder epithelial cells.
    1 context sentence
    Summary · context
    An EDTA procedure was used to prepare isolated epithelial cells of human gallbladder devoid of endogenous vasoactive intestinal peptide (VIP) as measured by radioimmunoassay.
  9. 09LabIn vitro (cells)Efficacy outcome

    In isolated human gallbladder epithelial cells, secretin also stimulated cyclic AMP production but with 10,000 times lower potency than VIP.

    In plain wordsIn cells from human gallbladder, secretin raised cyclic AMP about 10,000 times less strongly than VIP.

    Peptide
    Vasoactive intestinal peptide (VIP, aviptadil)
    Outcome
    Not applicableSecretin potency for stimulating cyclic AMP
    Study

    humann: not reported in source

    isolated gallbladder epithelial cells
    Comparator
    Secretin
    Outcome group
    Receptor activity and mechanism · Gastrointestinal
    Studied dose
    Dose not stated

    Source sentences

    Summary
    Secretin also stimulated cyclic AMP production but with a 10,000 lower potency than VIP.
    1 context sentence
    Summary · context
    An EDTA procedure was used to prepare isolated epithelial cells of human gallbladder devoid of endogenous vasoactive intestinal peptide (VIP) as measured by radioimmunoassay.
  10. 10LabIn vitro (cells)Efficacy outcome

    In guinea pig gallbladder epithelial cells, VIP and secretin were equipotent stimulators of cyclic AMP.

    In plain wordsIn guinea pig gallbladder cells, VIP and secretin raised cyclic AMP equally strongly.

    Peptide
    Vasoactive intestinal peptide (VIP, aviptadil)
    Outcome
    Not applicableVIP versus secretin on cyclic AMP in guinea pig gallbladder cells
    Study

    guinea pign: not reported in source

    gallbladder epithelial cells
    Comparator
    Secretin
    Outcome group
    Receptor activity and mechanism · Gastrointestinal
    Studied dose
    Dose not stated

    Source sentences

    Summary
    In the guinea pig, VIP and secretin were equipotent stimulators of cyclic AMP in gallbladder epithelial cells.
  11. 11LabIn vitro (cells)Mechanism

    In guinea pig gallbladder epithelial cells, equal VIP and secretin potency was due to separate receptors specific for each peptide.

    In plain wordsIn guinea pig gallbladder cells, VIP and secretin acted equally strongly because each peptide has its own receptor.

    Peptide
    Vasoactive intestinal peptide (VIP, aviptadil)
    Outcome
    Not applicablePeptide-specific receptors in guinea pig gallbladder cells
    Study

    guinea pign: not reported in source

    gallbladder epithelial cells
    Outcome group
    Receptor activity and mechanism · Gastrointestinal
    Studied dose
    Dose not stated

    Source sentences

    Summary
    In the guinea pig, VIP and secretin were equipotent stimulators of cyclic AMP in gallbladder epithelial cells. This particular feature was shown to be due to receptors specific for each peptide that were present in these cells.

AI wrote and checked these results. AI can make mistakes, so check important results against the source.

Version history

PepBank keeps every version of the data on this study. Status changes from Retraction Watch, Europe PMC and Crossref are listed with their source.

  1. Version 2Current

    12 Oct 2026, 12:58 UTC

    Study results updated

  2. Version 1

    9 Oct 2026, 12:41 UTC

    First added · source update from Europe PMC

Subjects

Terms as the source lists them.

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