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Study · In vitro (cell-free) · 1984

4 results

Evidence for peptide aggregation.

Kastin AJ, Castellanos PF, Fischman AJ, Proffitt JK, Graf MV

Pharmacology, biochemistry, and behavior1 Dec 1984Cited by 19 (Europe PMC)

PMID6151671 (opens in a new tab)DOI10.1016/s0091-3057(84)80082-9 (opens in a new tab)PepBankPUB-06151671
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Summary

The text of this summary is not shown here, because the article license does not allow reuse. Read it at the source (opens in a new tab)

Text rights: Publisher copyright. Results still quote short attributed sentences.

Results from this study4

4 results from studies that gave the peptide. No result from this study comes from people.

  1. 01LabIn vitro (cell-free)Mechanism

    In serum, labeled DSIP produced a first peak (A) that eluted at void volume, a position expected for peptide bound to protein.

    In plain wordsIn lab tests, labeled DSIP added to serum partly eluted where protein-bound peptide is expected.

    Peptide
    Delta sleep-inducing peptide (DSIP)
    Outcome
    Not applicableBinding of labeled DSIP to serum protein (peak A)
    Study

    n: not reported in source

    Serum with 125I-Tyr-DSIP, Sephadex G-25 gel filtration
    Outcome group
    Receptor activity and mechanism · Pharmacology
    Studied dose
    Dose not stated

    Source sentences

    Summary
    The first peak (A) eluted at void volume, a position expected for labeled peptide bound to protein.
    1 context sentence
    Summary · context
    Addition of 125I-Tyr-DSIP to serum resulted in four peaks after gel filtration chromatography on a column of Sephadex G-25.
  2. 02LabIn vitro (cell-free)Mechanism

    In serum, glacial acetic acid or the chelating agent 1,10-phenanthroline greatly reduced the smaller-size peaks B and D of labeled DSIP.

    In plain wordsIn lab tests, an acid or a metal-binding agent greatly reduced the smaller-size DSIP peaks seen in serum.

    Peptide
    Delta sleep-inducing peptide (DSIP)
    Outcome
    DecreaseEffect of acetic acid or 1,10-phenanthroline on DSIP serum peaks B and D
    Study

    n: not reported in source

    Serum with 125I-Tyr-DSIP, Sephadex G-25 gel filtration
    Outcome group
    Receptor activity and mechanism · Pharmacology
    Studied dose
    Dose not stated

    Source sentences

    Summary
    The other two peaks (B and D), corresponding to smaller molecular size material, were greatly reduced by addition of glacial acetic acid or the chelating agent 1,10-phenanthroline before or even after mixing of the peptide with serum.
    1 context sentence
    Summary · context
    Addition of 125I-Tyr-DSIP to serum resulted in four peaks after gel filtration chromatography on a column of Sephadex G-25.
  3. 03LabIn vitro (cell-free)Mechanism

    Iron interacted with labeled DSIP, and a mixture of ferric chloride and peptide without serum formed the additional peak B.

    In plain wordsIn lab tests, iron interacted with DSIP and, without serum, produced one of the extra peaks.

    Peptide
    Delta sleep-inducing peptide (DSIP)
    Outcome
    Not applicableInteraction of iron with labeled DSIP
    Study

    n: not reported in source

    Serum with 125I-Tyr-DSIP, Sephadex G-25 gel filtration
    Outcome group
    Receptor activity and mechanism · Pharmacology
    Studied dose
    Dose not stated

    Source sentences

    Summary
    Iron was one of the ions found to interact with 125I-Tyr-DSIP, and chromatography of a mixture of ferric chloride and peptide without serum resulted in the additional formation of peak B.
  4. 04LabIn vitro (cell-free)Mechanism

    A substantial portion of peaks A, B and C, but not D, reacted with a specific DSIP antibody, indicating intact peptide.

    In plain wordsIn lab tests, most DSIP peaks except one reacted with a DSIP antibody, which points to intact peptide.

    Peptide
    Delta sleep-inducing peptide (DSIP)
    Outcome
    Not applicableImmunoreactivity of DSIP serum peaks A, B, C and D
    Study

    n: not reported in source

    Serum with 125I-Tyr-DSIP, Sephadex G-25 gel filtration
    Outcome group
    Receptor activity and mechanism · Pharmacology
    Studied dose
    Dose not stated

    Source sentences

    Summary
    A substantial portion of peaks A, B, and C (but not D) reacted with a specific DSIP antibody, indicating the presence of intact peptide.
    1 context sentence
    Summary · context
    Addition of 125I-Tyr-DSIP to serum resulted in four peaks after gel filtration chromatography on a column of Sephadex G-25.

AI wrote and checked these results. AI can make mistakes, so check important results against the source.

Version history

PepBank keeps every version of the data on this study. Status changes from Retraction Watch, Europe PMC and Crossref are listed with their source.

  1. Version 2Current

    10 Oct 2026, 09:43 UTC

    Study results updated

  2. Version 1

    9 Oct 2026, 03:04 UTC

    First added · source update from Europe PMC

Subjects

Terms as the source lists them.

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