Purification of [125I]-vasoactive intestinal peptide by reverse-phase HPLC.

Paul S, Wood K, Said SI

Peptides1 Nov 1984Cited by 19 (Europe PMC)

PMID6099559DOI10.1016/0196-9781(84)90175-xPepBankPUB-06099559

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Summary

The text of this summary is not shown here, because the article license does not allow reuse. Read it at the source (opens in a new tab)

Text rights: Publisher copyright. Results still quote short attributed sentences.

Results from this study2

No result from this study comes from a test of the peptide.

Studies that did not test the peptide alone2

In this study, Vasoactive intestinal peptide (VIP, aviptadil) was a lab tool or a carrier, part of a mix, or the subject of a lab method. These results are not evidence about taking Vasoactive intestinal peptide (VIP, aviptadil).

01LabNot a test of Vasoactive intestinal peptide (VIP, aviptadil) aloneIn vitro (cell-free)Other
Reverse-phase HPLC gave 125I-VIP with a specific activity of 1.99 +/- 0.21 Ci/mumole, close to the maximum for monoiodinated VIP.
In plain wordsIn lab work, purified radiolabelled VIP reached a specific activity close to the maximum possible.
Peptide
Vasoactive intestinal peptide (VIP, aviptadil)
Outcome
Not applicableSpecific activity of HPLC-purified 125I-VIP
Study
n: not reported in source
125I-VIP preparation
Outcome group
Analytical methods and detection · Pharmacology
Measurements
specific activity
1.99 Ci/mumole
125I-VIP
Source sentences
Summary
The specific activity of the 125I-VIP was 1.99 +/- 0.21 Ci/mumole, approaching the maximum specific activity of monoiodinated VIP (2.26 Ci/mumole).
In this study, Vasoactive intestinal peptide (VIP, aviptadil) was a lab tool or a carrier, part of a mix, or the subject of a lab method. This result is not evidence about taking Vasoactive intestinal peptide (VIP, aviptadil).
Report a problemFND-0609955901
02LabNot a test of Vasoactive intestinal peptide (VIP, aviptadil) aloneIn vitro (cell-free)Other
Radioimmunoassay and radioreceptorassay for VIP were 2.6-fold and 2.5-fold more sensitive with HPLC-purified 125I-VIP than with 125I-VIP from an open-end cellulose column.
In plain wordsIn lab tests, VIP assays were more sensitive with HPLC-purified radiolabelled VIP than with VIP from a cellulose column.
Peptide
Vasoactive intestinal peptide (VIP, aviptadil)
Outcome
IncreaseSensitivity of radioimmunoassay and radioreceptorassay for VIP
Study
n: not reported in source
125I-VIP preparation
Comparator
125I-VIP from an open-end cellulose column
Outcome group
Analytical methods and detection · Pharmacology
Measurements
radioimmunoassay sensitivity gain
2.6-fold
HPLC-purified vs cellulose column
radioreceptorassay sensitivity gain
2.5-fold
HPLC-purified vs cellulose column
Source sentences
Summary
Radioimmunoassay and radioreceptorassay for VIP were more sensitive (2.6-fold, and 2.5-fold, respectively) using 125I-VIP purified by HPLC compared to 125I-VIP obtained from an open-end cellulose column.
In this study, Vasoactive intestinal peptide (VIP, aviptadil) was a lab tool or a carrier, part of a mix, or the subject of a lab method. This result is not evidence about taking Vasoactive intestinal peptide (VIP, aviptadil).
Report a problemFND-0609955902

AI wrote and checked these results. AI can make mistakes, so check important results against the source.

Version history

PepBank keeps every version of the data on this study. Status changes from Retraction Watch, Europe PMC and Crossref are listed with their source.

Version 2Current
12 Oct 2026, 12:58 UTC
Study results updated
Version 1
9 Oct 2026, 12:41 UTC
First added · source update from Europe PMC

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