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Study · In vitro (cells) · chick · 1984

5 results

Elevation of intracellular cyclic AMP and stimulation of adenylate cyclase activity by vasoactive intestinal peptide and glucagon in the retinal pigment epithelium.

Koh SW, Chader GJ

Journal of neurochemistry1 Dec 1984Cited by 23 (Europe PMC)

PMID6092540 (opens in a new tab)DOI10.1111/j.1471-4159.1984.tb06072.x (opens in a new tab)PepBankPUB-06092540
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Summary

The text of this summary is not shown here, because the article license does not allow reuse. Read it at the source (opens in a new tab)

Text rights: Publisher copyright. Results still quote short attributed sentences.

Results from this study5

5 results from studies that gave the peptide. No result from this study comes from people.

  1. 01LabIn vitro (cells)Activity measurement

    VIP rapidly raised cyclic AMP levels in embryonic chick retinal pigment epithelium, in culture and in freshly dissected tissue.

    In plain wordsIn chick eye tissue and cells, VIP quickly raised cyclic AMP levels.

    Peptide
    Vasoactive intestinal peptide (VIP, aviptadil)
    Outcome
    IncreaseIntracellular cyclic AMP level
    Study

    chickn: not reported in source

    embryonic chick retinal pigment epithelium
    Outcome group
    Receptor activity and mechanism · Eye
    Studied dose
    Dose not stated

    Source sentences

    Summary
    Both vasoactive intestinal peptide (VIP) and glucagon rapidly elevated cyclic AMP levels in embryonic chick retinal pigment epithelium (RPE), in culture as well as in freshly dissected tissue.
  2. 02LabIn vitro (cells)Activity measurement

    In cultured chick retinal pigment epithelium cells, VIP had a half-maximal activity of 5 X 10(-8) M.

    In plain wordsIn cultured chick eye cells, VIP worked at a half-maximal level of 5 X 10(-8) M.

    Peptide
    Vasoactive intestinal peptide (VIP, aviptadil)
    Outcome
    IncreaseHalf-maximal activity of VIP on cyclic AMP in cultured cells
    Study

    chickn: not reported in source

    embryonic chick retinal pigment epithelium
    Outcome group
    Receptor activity and mechanism · Eye
    Studied dose
    Dose not stated

    Source sentences

    Summary
    In cultured cells, the half-maximal activities of VIP and glucagon were 5 X 10(-8) M and 3 X 10(-8) M, respectively.
  3. 03LabIn vitro (cells)Activity measurement

    After 3 min of reaction, VIP raised intracellular cyclic AMP by 100-fold in chick retinal pigment epithelium.

    In plain wordsIn chick eye cells, VIP raised cyclic AMP 100-fold within 3 minutes.

    Peptide
    Vasoactive intestinal peptide (VIP, aviptadil)
    Outcome
    IncreaseCyclic AMP elevation after 3 min
    Study

    chickn: not reported in source

    embryonic chick retinal pigment epithelium
    Outcome group
    Receptor activity and mechanism · Eye
    Studied dose
    Dose not stated

    Measurements

    cyclic AMP elevation
    100-fold
    VIP, 3 min

    Source sentences

    Summary
    After 3 min of reaction, VIP elevated intracellular cyclic AMP by 100-fold; elevation with glucagon was up to 10-fold.
  4. 04LabIn vitro (cells)Activity measurement

    VIP stimulated adenylate cyclase activity in chick retinal pigment epithelium membranes.

    In plain wordsIn chick eye cell membranes, VIP stimulated adenylate cyclase activity.

    Peptide
    Vasoactive intestinal peptide (VIP, aviptadil)
    Outcome
    IncreaseAdenylate cyclase activity in RPE membranes
    Study

    chickn: not reported in source

    RPE membranes
    Outcome group
    Receptor activity and mechanism · Eye
    Studied dose
    Dose not stated

    Source sentences

    Summary
    Both neuropeptides stimulated adenylate cyclase activity in RPE membranes.
  5. 05LabIn vitro (cells)Activity measurement

    VIP was more effective than glucagon in stimulating adenylate cyclase activity in chick retinal pigment epithelium membranes, with a dose-response curve shifted to higher concentrations.

    In plain wordsIn chick eye cell membranes, VIP stimulated adenylate cyclase more than glucagon did.

    Peptide
    Vasoactive intestinal peptide (VIP, aviptadil)
    Outcome
    IncreaseAdenylate cyclase stimulation, VIP versus glucagon
    Study

    chickn: not reported in source

    RPE membranes
    Comparator
    Glucagon
    Outcome group
    Receptor activity and mechanism · Eye
    Studied dose
    Dose not stated

    Source sentences

    Summary
    VIP remained more effective than glucagon in stimulating adenylate cyclase activity, but the dose-response curve was shifted to a higher concentration range when compared to that of the VIP-elevated intracellular cyclic AMP.

AI wrote and checked these results. AI can make mistakes, so check important results against the source.

Version history

PepBank keeps every version of the data on this study. Status changes from Retraction Watch, Europe PMC and Crossref are listed with their source.

  1. Version 2Current

    12 Oct 2026, 12:58 UTC

    Study results updated

  2. Version 1

    9 Oct 2026, 12:41 UTC

    First added · source update from Europe PMC

Subjects

Terms as the source lists them.

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