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Study · In vitro (cells) · 1984

3 results

Activation of the cAMP-generating system by vasoactive intestinal polypeptide (VIP) in the human laryngeal malignant cell line HEp-2.

Prost A, Emami S, Rosselin G, Gespach C

Bioscience reports1 Dec 1984Cited by 5 (Europe PMC)

PMID6085015 (opens in a new tab)DOI10.1007/bf01116697 (opens in a new tab)PepBankPUB-06085015
Publisher pagePubMedAsk about this study
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Summary

The text of this summary is not shown here, because the article license does not allow reuse. Read it at the source (opens in a new tab)

Text rights: Publisher copyright. Results still quote short attributed sentences.

Results from this study3

3 results from studies that gave the peptide. No result from this study comes from people.

  1. 01LabIn vitro (cells)Activity measurement

    In human laryngeal HEp-2 cells, VIP produced a dose-related, 8-fold increase in cAMP production.

    In plain wordsIn human throat tumour cells in culture, VIP raised cAMP about 8-fold, with larger doses giving larger rises.

    Peptide
    Vasoactive intestinal peptide (VIP, aviptadil)
    Outcome
    IncreasecAMP production in HEp-2 cells
    Study

    humann: not reported in source

    HEp-2 human laryngeal malignant cell line
    Outcome group
    Receptor activity and mechanism · Respiratory
    Studied dose
    3 X 10(-9)-10(-7) MAs given in this study (human).

    Measurements

    cAMP production increase
    8 fold
    HEp-2 cells

    Source sentences

    Summary
    In the presence of 3-isobutyl-l-methylxanthine, VIP produced a dose-related (3 X 10(-9)-10(-7) M) increase (8-fold) in cAMP production in isolated HEp-2 cells incubated at 15 degrees C in KRP buffer.
  2. 02LabIn vitro (cells)Activity measurement

    In human laryngeal HEp-2 cells, among peptides related to VIP only PHI activated the cAMP-generating system, at much lower potency than VIP.

    In plain wordsIn human throat tumour cells in culture, VIP activated cAMP production far more strongly than related peptides; only PHI worked at all.

    Peptide
    Vasoactive intestinal peptide (VIP, aviptadil)
    Outcome
    Not applicableVIP versus structurally related peptides for cAMP activation
    Study

    humann: not reported in source

    HEp-2 human laryngeal malignant cell line
    Comparator
    Secretin, glucagon, PHI, somatostatin-14, hpGRF, GIP
    Outcome group
    Receptor activity and mechanism · Respiratory
    Studied dose
    Dose not stated

    Source sentences

    Summary
    Among the peptides structurally related to VIP, including secretin (10(-7) M), pancreatic glucagon (10(-6) M), PHI, somatostatin-14 (10(-6) M), hpGRF (10(-8)-4 X 10(-6) M), GIP (2 X 10(-7) M), only PHI (3 X 10(-7) M and above) is able to activate the cAMP-generating system in HEp-2 cells, but at 10(…
    1 context sentence
    Summary · context
    In the presence of 3-isobutyl-l-methylxanthine, VIP produced a dose-related (3 X 10(-9)-10(-7) M) increase (8-fold) in cAMP production in isolated HEp-2 cells incubated at 15 degrees C in KRP buffer.
  3. 03LabIn vitro (cells)Mechanism

    In human laryngeal HEp-2 cells, the VIP effect relates to VIP recognition sites (125I-VIP binding) coupled to membrane-bound adenylate cyclase.

    In plain wordsIn human throat tumour cells in culture, VIP acted through binding sites linked to the enzyme that makes cAMP.

    Peptide
    Vasoactive intestinal peptide (VIP, aviptadil)
    Outcome
    Not applicableVIP recognition sites coupled to adenylate cyclase
    Study

    humann: not reported in source

    HEp-2 human laryngeal malignant cell line
    Outcome group
    Receptor activity and mechanism · Respiratory
    Studied dose
    Dose not stated

    Source sentences

    Summary
    The VIP effect is related to the interaction of the peptide on VIP recognition sites (125I-VIP-binding capacity), coupled to the membrane-bound adenylate cyclase.
    1 context sentence
    Summary · context
    In the presence of 3-isobutyl-l-methylxanthine, VIP produced a dose-related (3 X 10(-9)-10(-7) M) increase (8-fold) in cAMP production in isolated HEp-2 cells incubated at 15 degrees C in KRP buffer.

AI wrote and checked these results. AI can make mistakes, so check important results against the source.

Version history

PepBank keeps every version of the data on this study. Status changes from Retraction Watch, Europe PMC and Crossref are listed with their source.

  1. Version 2Current

    12 Oct 2026, 12:58 UTC

    Study results updated

  2. Version 1

    9 Oct 2026, 12:41 UTC

    First added · source update from Europe PMC

Subjects

Terms as the source lists them.

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