Skip to content

Study · In vitro (cells) · rat · 1985

6 results

Characterization of an osteoblast-like clonal cell line which responds to both parathyroid hormone and calcitonin.

Forrest SM, Ng KW, Findlay DM, Michelangeli VP, Livesey SA, Partridge NC, Zajac JD, Martin TJ

Calcified tissue international1 Jan 1985Cited by 123 (Europe PMC)

PMID3922597 (opens in a new tab)DOI10.1007/bf02557679 (opens in a new tab)PepBankPUB-03922597
Publisher pagePubMedAsk about this study
Jump to 6 results

Summary

The text of this summary is not shown here, because the article license does not allow reuse. Read it at the source (opens in a new tab)

Text rights: Publisher copyright. Results still quote short attributed sentences.

Results from this study6

6 results from studies that gave the peptide. No result from this study comes from people.

  1. 01LabIn vitro (cells)Mechanism

    In rat-derived UMR 106-06 cells, 3 X 10(-8)M salmon calcitonin stimulated adenylate cyclase sevenfold.

    In plain wordsIn lab tests, salmon calcitonin stimulated adenylate cyclase sevenfold in rat-derived UMR 106-06 cells.

    Peptide
    Calcitonin-salmon
    Outcome
    IncreaseAdenylate cyclase stimulation
    Study

    ratn: not reported in source

    UMR 106-06 osteoblast-like cells
    Outcome group
    Receptor activity and mechanism · Pharmacology
    Studied dose
    3 X 10(-8)MAs given in this study (rat).

    Source sentences

    Summary
    At 3 X 10(-8)M SCT, there was a sevenfold stimulation of adenylate cyclase, 84% activation of isoenzyme I, and 44% activation of isoenzyme II.
    2 context sentences
    Summary · context
    The clonal cell line UMR 106, which was originally derived from a rat transplantable osteogenic sarcoma with an osteoblastic phenotype, was subcloned after the emergence of a calcitonin-responsive adenylate cyclase was noted in late passages.
    Summary · context
    Detailed studies on the stimulation of adenylate cyclase and activation profile of the cyclic AMP-dependent protein kinase isoenzymes in response to parathyroid hormone (PTH) and salmon calcitonin (SCT) were conducted on two subclones (UMR 106-01 and UMR 106-06).
  2. 02LabIn vitro (cells)Mechanism

    In rat-derived UMR 106-06 cells, 3 X 10(-8)M salmon calcitonin produced 84% activation of protein kinase isoenzyme I.

    In plain wordsIn lab tests, salmon calcitonin produced 84% activation of protein kinase isoenzyme I in rat-derived UMR 106-06 cells.

    Peptide
    Calcitonin-salmon
    Outcome
    IncreaseProtein kinase isoenzyme I activation
    Study

    ratn: not reported in source

    UMR 106-06 osteoblast-like cells
    Outcome group
    Receptor activity and mechanism · Pharmacology
    Studied dose
    3 X 10(-8)MAs given in this study (rat).

    Source sentences

    Summary
    At 3 X 10(-8)M SCT, there was a sevenfold stimulation of adenylate cyclase, 84% activation of isoenzyme I, and 44% activation of isoenzyme II.
    2 context sentences
    Summary · context
    Detailed studies on the stimulation of adenylate cyclase and activation profile of the cyclic AMP-dependent protein kinase isoenzymes in response to parathyroid hormone (PTH) and salmon calcitonin (SCT) were conducted on two subclones (UMR 106-01 and UMR 106-06).
    Summary · context
    The clonal cell line UMR 106, which was originally derived from a rat transplantable osteogenic sarcoma with an osteoblastic phenotype, was subcloned after the emergence of a calcitonin-responsive adenylate cyclase was noted in late passages.
  3. 03LabIn vitro (cells)Mechanism

    In rat-derived UMR 106-06 cells, 3 X 10(-8)M salmon calcitonin produced 44% activation of protein kinase isoenzyme II.

    In plain wordsIn lab tests, salmon calcitonin produced 44% activation of protein kinase isoenzyme II in rat-derived UMR 106-06 cells.

    Peptide
    Calcitonin-salmon
    Outcome
    IncreaseProtein kinase isoenzyme II activation
    Study

    ratn: not reported in source

    UMR 106-06 osteoblast-like cells
    Outcome group
    Receptor activity and mechanism · Pharmacology
    Studied dose
    3 X 10(-8)MAs given in this study (rat).

    Source sentences

    Summary
    At 3 X 10(-8)M SCT, there was a sevenfold stimulation of adenylate cyclase, 84% activation of isoenzyme I, and 44% activation of isoenzyme II.
    2 context sentences
    Summary · context
    Detailed studies on the stimulation of adenylate cyclase and activation profile of the cyclic AMP-dependent protein kinase isoenzymes in response to parathyroid hormone (PTH) and salmon calcitonin (SCT) were conducted on two subclones (UMR 106-01 and UMR 106-06).
    Summary · context
    The clonal cell line UMR 106, which was originally derived from a rat transplantable osteogenic sarcoma with an osteoblastic phenotype, was subcloned after the emergence of a calcitonin-responsive adenylate cyclase was noted in late passages.
  4. 04LabIn vitro (cells)Mechanism

    In rat-derived osteoblast-like cells, only UMR 106-06, rather than UMR 106-01, responded to calcitonin.

    In plain wordsIn lab tests, only rat-derived UMR 106-06 cells responded to calcitonin, while UMR 106-01 cells did not.

    Peptide
    Calcitonin-salmon
    Outcome
    MixedSubclone calcitonin responsiveness
    Study

    ratn: not reported in source

    UMR 106-01 and UMR 106-06
    Comparator
    UMR 106-01
    Outcome group
    Receptor activity and mechanism · Pharmacology
    Studied dose
    Dose not stated

    Source sentences

    Summary
    In contrast, only UMR 106-06 cells responded to calcitonin.
    2 context sentences
    Summary · context
    Detailed studies on the stimulation of adenylate cyclase and activation profile of the cyclic AMP-dependent protein kinase isoenzymes in response to parathyroid hormone (PTH) and salmon calcitonin (SCT) were conducted on two subclones (UMR 106-01 and UMR 106-06).
    Summary · context
    The clonal cell line UMR 106, which was originally derived from a rat transplantable osteogenic sarcoma with an osteoblastic phenotype, was subcloned after the emergence of a calcitonin-responsive adenylate cyclase was noted in late passages.
  5. 05LabIn vitro (cells)Mechanism

    In rat-derived UMR 106-06 cells, protein kinase isoenzyme activation profiles were similar with salmon calcitonin and PTH.

    In plain wordsIn lab tests, salmon calcitonin and PTH produced similar protein kinase activation profiles in rat-derived UMR 106-06 cells.

    Peptide
    Calcitonin-salmon
    Outcome
    No significant changeProtein kinase activation profiles
    Study

    ratn: not reported in source

    UMR 106-06
    Comparator
    PTH
    Outcome group
    Receptor activity and mechanism · Pharmacology
    Studied dose
    Dose not stated

    Source sentences

    Summary
    The activation profiles of the isoenzymes to PTH and SCT in UMR 106-06 were similar.
    2 context sentences
    Summary · context
    Detailed studies on the stimulation of adenylate cyclase and activation profile of the cyclic AMP-dependent protein kinase isoenzymes in response to parathyroid hormone (PTH) and salmon calcitonin (SCT) were conducted on two subclones (UMR 106-01 and UMR 106-06).
    Summary · context
    The clonal cell line UMR 106, which was originally derived from a rat transplantable osteogenic sarcoma with an osteoblastic phenotype, was subcloned after the emergence of a calcitonin-responsive adenylate cyclase was noted in late passages.
  6. 06LabIn vitro (cells)Activity measurement

    In rat-derived UMR 106-06 cells, specific saturable salmon calcitonin binding had apparent Kd 0.8 nM and 6,000 receptors/cell.

    In plain wordsIn lab tests, rat-derived UMR 106-06 cells showed specific, saturable salmon calcitonin binding with apparent Kd 0.8 nM and 6,000 receptors/cell.

    Peptide
    Calcitonin-salmon
    Outcome
    Not applicableSalmon calcitonin receptor binding
    Study

    ratn: not reported in source

    UMR 106-06
    Outcome group
    Receptor activity and mechanism · Pharmacology
    Studied dose
    Dose not stated

    Source sentences

    Summary
    Binding parameters indicate apparent Kd = 0.8 nM and 6,000 receptors/cell.
    2 context sentences
    Summary · context
    Furthermore, their response to SCT correlates with the presence of specific, saturable binding of 125I-labeled SCT.
    Summary · context
    The clonal cell line UMR 106, which was originally derived from a rat transplantable osteogenic sarcoma with an osteoblastic phenotype, was subcloned after the emergence of a calcitonin-responsive adenylate cyclase was noted in late passages.

AI wrote and checked these results. AI can make mistakes, so check important results against the source.

Version history

PepBank keeps every version of the data on this study. Status changes from Retraction Watch, Europe PMC and Crossref are listed with their source.

  1. Version 2Current

    28 Oct 2026, 02:03 UTC

    Study results updated

  2. Version 1

    27 Oct 2026, 16:35 UTC

    First added · source update from Europe PMC

Subjects

Terms as the source lists them.

PepBank is a research reference. Talk to a doctor before you use any peptide.