Parallel studies of His-DTrp-Ala-Trp-DPhe-Lys-NH2 and human pancreatic growth hormone-releasing factor-44-NH2 in rat primary pituitary cell monolayer culture.

Sartor O, Bowers CY, Chang D

Endocrinology1 Mar 1985Cited by 54 (Europe PMC)

PMID3918849DOI10.1210/endo-116-3-952PepBankPUB-03918849

Publisher page PubMedAsk about this study

Summary

The text of this summary is not shown here, because the article license does not allow reuse. Read it at the source (opens in a new tab)

Text rights: Publisher copyright. Results still quote short attributed sentences.

Results from this study9

4 results from studies that gave the peptide. No result from this study comes from people.

01LabIn vitro (cells)Efficacy outcome
In rat pituitary cultures, GH-RP-6 released GH in a dose- and time-dependent manner.
In plain wordsIn cells from rats, GH-RP-6 stimulated growth hormone release according to dose and exposure time.
Peptide
GHRP-6
Outcome
IncreaseGH release
Study
ratn: not reported in source
primary pituitary cell monolayer culture
Outcome group
Growth hormone and IGF-1 · Endocrine and hormones
Studied dose
Dose not stated
Source sentences
Summary
Both peptides released GH in a dose- and time-dependent manner.
3 context sentences(design, population, supporting numbers)
Title · context
Parallel studies of His-DTrp-Ala-Trp-DPhe-Lys-NH2 and human pancreatic growth hormone-releasing factor-44-NH2 in rat primary pituitary cell monolayer culture.
Summary · context
In this study, for the first time, GH-RP-6 was studied in primary pituitary cell monolayer culture.
Summary · context
Parallel studies were performed with human pancreatic GH-releasing factor-44 (hpGRF-44).
Report a problemFND-0391884902
02LabIn vitro (cells)Activity measurement
In rat pituitary cultures, the ED50 for GH-RP-6 was 9 nM.
In plain wordsIn cells from rats, GH-RP-6 had a half-maximal effective concentration of 9 nM.
Peptide
GHRP-6
Outcome
Not applicableGH release ED50
Study
ratn: not reported in source
primary pituitary cell monolayer culture
Outcome group
Growth hormone and IGF-1 · Endocrine and hormones
Studied dose
Dose not stated
Measurements
ED50
9 nM
GH-RP-6
Source sentences
Summary
In this assay system, the ED50 for GH-RP-6 was 9 nM, and the ED50 for hp-GRF-44 was 1.6 nM.
3 context sentences(design, population, supporting numbers)
Title · context
Parallel studies of His-DTrp-Ala-Trp-DPhe-Lys-NH2 and human pancreatic growth hormone-releasing factor-44-NH2 in rat primary pituitary cell monolayer culture.
Summary · context
In this study, for the first time, GH-RP-6 was studied in primary pituitary cell monolayer culture.
Summary · context
Parallel studies were performed with human pancreatic GH-releasing factor-44 (hpGRF-44).
Report a problemFND-0391884903
03LabIn vitro (cells)Efficacy outcome
In rat pituitary cultures, GH-RP-6 pretreatment decreased the subsequent response to GH-RP-6.
In plain wordsIn cells from rats, prior GH-RP-6 exposure reduced the response to later GH-RP-6 exposure.
Peptide
GHRP-6
Outcome
DecreaseSubsequent GH-RP-6 response
Study
ratn: not reported in source
primary pituitary cell monolayer culture
Outcome group
Growth hormone and IGF-1 · Endocrine and hormones
Studied dose
Dose not stated
Source sentences
Summary
Pretreatment with GH-RP-6 decreased the subsequent response to both GH-RP-6 and hpGRF-44.
3 context sentences(design, population, supporting numbers)
Title · context
Parallel studies of His-DTrp-Ala-Trp-DPhe-Lys-NH2 and human pancreatic growth hormone-releasing factor-44-NH2 in rat primary pituitary cell monolayer culture.
Summary · context
In this study, for the first time, GH-RP-6 was studied in primary pituitary cell monolayer culture.
Summary · context
Parallel studies were performed with human pancreatic GH-releasing factor-44 (hpGRF-44).
Report a problemFND-0391884905
04LabIn vitro (cells)Efficacy outcome
In rat pituitary cultures, GH-RP-6 pretreatment decreased the subsequent response to hpGRF-44.
In plain wordsIn cells from rats, prior GH-RP-6 exposure reduced the later growth hormone response to hpGRF-44.
Peptide
GHRP-6
Outcome
DecreaseSubsequent hpGRF-44 response
Study
ratn: not reported in source
primary pituitary cell monolayer culture
Outcome group
Growth hormone and IGF-1 · Endocrine and hormones
Studied dose
Dose not stated
Source sentences
Summary
Pretreatment with GH-RP-6 decreased the subsequent response to both GH-RP-6 and hpGRF-44.
3 context sentences(design, population, supporting numbers)
Title · context
Parallel studies of His-DTrp-Ala-Trp-DPhe-Lys-NH2 and human pancreatic growth hormone-releasing factor-44-NH2 in rat primary pituitary cell monolayer culture.
Summary · context
In this study, for the first time, GH-RP-6 was studied in primary pituitary cell monolayer culture.
Summary · context
Parallel studies were performed with human pancreatic GH-releasing factor-44 (hpGRF-44).
Report a problemFND-0391884906

Studies that did not test the peptide alone5

In this study, GHRP-6 was a lab tool or a carrier, part of a mix, or the subject of a lab method. These results are not evidence about taking GHRP-6.

05LabNot a test of GHRP-6 aloneIn vitro (cells)Efficacy outcome
In rat pituitary cultures, conditions optimal for GH-RP-6 were not optimal for hpGRF-44.
In plain wordsIn cells from rats, optimal culture conditions differed between GH-RP-6 and hpGRF-44.
Peptide
GHRP-6
Outcome
Not applicableOptimal culture conditions
Study
ratn: not reported in source
primary pituitary cell monolayer culture
Outcome group
Growth hormone and IGF-1 · Endocrine and hormones
Source sentences
Summary
Culture conditions optimal for GH-RP-6 were not optimal for hpGRF-44.
3 context sentences(design, population, supporting numbers)
Title · context
Parallel studies of His-DTrp-Ala-Trp-DPhe-Lys-NH2 and human pancreatic growth hormone-releasing factor-44-NH2 in rat primary pituitary cell monolayer culture.
Summary · context
In this study, for the first time, GH-RP-6 was studied in primary pituitary cell monolayer culture.
Summary · context
Parallel studies were performed with human pancreatic GH-releasing factor-44 (hpGRF-44).
In this study, GHRP-6 was a lab tool or a carrier, part of a mix, or the subject of a lab method. This result is not evidence about taking GHRP-6.
Report a problemFND-0391884901
06LabNot a test of GHRP-6 aloneIn vitro (cells)Mechanism
In rat pituitary cultures, calcium-blocking agents inhibited the GH response to GH-RP-6.
In plain wordsIn cells from rats, calcium blockers reduced the growth hormone response to GH-RP-6.
Peptide
GHRP-6
Outcome
DecreaseCalcium blocker effect on GH response
Study
ratn: not reported in source
primary pituitary cell monolayer culture
Outcome group
Growth hormone and IGF-1 · Endocrine and hormones
Source sentences
Summary
Calcium-blocking agents inhibited the GH responses of both peptides as well as basal GH release.
3 context sentences(design, population, supporting numbers)
Title · context
Parallel studies of His-DTrp-Ala-Trp-DPhe-Lys-NH2 and human pancreatic growth hormone-releasing factor-44-NH2 in rat primary pituitary cell monolayer culture.
Summary · context
In this study, for the first time, GH-RP-6 was studied in primary pituitary cell monolayer culture.
Summary · context
Parallel studies were performed with human pancreatic GH-releasing factor-44 (hpGRF-44).
In this study, GHRP-6 was a lab tool or a carrier, part of a mix, or the subject of a lab method. This result is not evidence about taking GHRP-6.
Report a problemFND-0391884904
07LabNot a test of GHRP-6 aloneIn vitro (cells)Mechanism
In rat pituitary cultures, hpGRF-44 pretreatment did not downregulate the GH-RP-6 response.
In plain wordsIn cells from rats, prior hpGRF-44 exposure did not reduce the GH-RP-6 response.
Peptide
GHRP-6
Outcome
No significant changehpGRF-44 pretreatment effect on GH-RP-6 response
Study
ratn: not reported in source
primary pituitary cell monolayer culture
Outcome group
Growth hormone and IGF-1 · Endocrine and hormones
Source sentences
Summary
hpGRF-44 down regulated itself but not GH-RP-6.
3 context sentences(design, population, supporting numbers)
Title · context
Parallel studies of His-DTrp-Ala-Trp-DPhe-Lys-NH2 and human pancreatic growth hormone-releasing factor-44-NH2 in rat primary pituitary cell monolayer culture.
Summary · context
In this study, for the first time, GH-RP-6 was studied in primary pituitary cell monolayer culture.
Summary · context
Parallel studies were performed with human pancreatic GH-releasing factor-44 (hpGRF-44).
In this study, GHRP-6 was a lab tool or a carrier, part of a mix, or the subject of a lab method. This result is not evidence about taking GHRP-6.
Report a problemFND-0391884907
08LabNot a test of GHRP-6 aloneIn vitro (cells)Mechanism
In rat pituitary cultures, rat sera did not potentiate the GH response to GH-RP-6.
In plain wordsIn cells from rats, rat sera did not strengthen the growth hormone response to GH-RP-6.
Peptide
GHRP-6
Outcome
No significant changeRat serum effect on GH-RP-6 response
Study
ratn: not reported in source
primary pituitary cell monolayer culture
Outcome group
Growth hormone and IGF-1 · Endocrine and hormones
Source sentences
Summary
Rat sera potentiated the GH response of hpGRF-44 but not that of GH-RP-6.
3 context sentences(design, population, supporting numbers)
Title · context
Parallel studies of His-DTrp-Ala-Trp-DPhe-Lys-NH2 and human pancreatic growth hormone-releasing factor-44-NH2 in rat primary pituitary cell monolayer culture.
Summary · context
In this study, for the first time, GH-RP-6 was studied in primary pituitary cell monolayer culture.
Summary · context
Parallel studies were performed with human pancreatic GH-releasing factor-44 (hpGRF-44).
In this study, GHRP-6 was a lab tool or a carrier, part of a mix, or the subject of a lab method. This result is not evidence about taking GHRP-6.
Report a problemFND-0391884908
09LabNot a test of GHRP-6 aloneIn vitro (cells)Mechanism
In rat pituitary cultures, GH-RP-6 and hpGRF-44 produced additive GH responses.
In plain wordsIn cells from rats, GH-RP-6 and hpGRF-44 produced additive growth hormone responses.
Peptide
GHRP-6
Outcome
IncreaseCombined GH response
Study
ratn: not reported in source
primary pituitary cell monolayer culture
Outcome group
Growth hormone and IGF-1 · Endocrine and hormones
Source sentences
Summary
GH-RP-6 and hpGRF-44 GH responses were additive.
3 context sentences(design, population, supporting numbers)
Title · context
Parallel studies of His-DTrp-Ala-Trp-DPhe-Lys-NH2 and human pancreatic growth hormone-releasing factor-44-NH2 in rat primary pituitary cell monolayer culture.
Summary · context
In this study, for the first time, GH-RP-6 was studied in primary pituitary cell monolayer culture.
Summary · context
Parallel studies were performed with human pancreatic GH-releasing factor-44 (hpGRF-44).
In this study, GHRP-6 was a lab tool or a carrier, part of a mix, or the subject of a lab method. This result is not evidence about taking GHRP-6.
Report a problemFND-0391884909

AI wrote and checked these results. AI can make mistakes, so check important results against the source.

Version history

PepBank keeps every version of the data on this study. Status changes from Retraction Watch, Europe PMC and Crossref are listed with their source.

Version 2Current
21 Oct 2026, 15:59 UTC
Study results updated
Version 1
9 Oct 2026, 03:13 UTC
First added · source update from Europe PMC

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